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超声波辅助花粉介导法获得转BtCry1Ac基因玉米的研究 被引量:3
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作者 南芝润 惠国强 +4 位作者 罗绮 宋红霞 吴家和 孙毅 张红梅 《黑龙江农业科学》 2015年第9期22-25,共4页
为了进一步分析玉米基因功能及基因转化,并为玉米抗虫提供种质资源,利用超声波辅助花粉介导法,将抗虫基因Cry1Ac导入玉米优良自交系昌7-2中,获得大量的转基因植株。结果表明:转基因植株经过草铵膦抗性筛选、PCR检测、BT蛋白试纸条检测... 为了进一步分析玉米基因功能及基因转化,并为玉米抗虫提供种质资源,利用超声波辅助花粉介导法,将抗虫基因Cry1Ac导入玉米优良自交系昌7-2中,获得大量的转基因植株。结果表明:转基因植株经过草铵膦抗性筛选、PCR检测、BT蛋白试纸条检测和室内抗虫性鉴定等分析,最后获得高抗玉米螟的转BtCry1Ac基因株系9个。培育出了高抗玉米螟的种质资源材料,同时也建立了超声波辅助花粉介导转化玉米的高效转化体系,该方法是简捷、快速有效、无基因型限制的植物转化方法。 展开更多
关键词 玉米 CRY1AC基因 超声波处理花粉介导 抗虫性
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三种全血基因组DNA提取方法的比较 被引量:15
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作者 杨泽民 罗少洪 陈耕夫 《中国实用医药》 2009年第12期13-14,共2页
目的比较高盐法、改良高盐法和超声波处理法三种方法对全血基因组DNA的提取效果。方法取200μl肝素抗凝血,分别采用上述三种方法提取基因组DNA,用紫外分光光度计和琼脂糖凝胶电泳检测其纯度和含量。结果三种方法提取的基因组DNA含量分别... 目的比较高盐法、改良高盐法和超声波处理法三种方法对全血基因组DNA的提取效果。方法取200μl肝素抗凝血,分别采用上述三种方法提取基因组DNA,用紫外分光光度计和琼脂糖凝胶电泳检测其纯度和含量。结果三种方法提取的基因组DNA含量分别为:高盐法38μg/ml全血;改良高盐法76.5μg/ml全血;超声波处理法20μg/ml全血;三种方法提取的基因组DNA纯度用A260/A280表示分别为:高盐法1.62;改良高盐法1.46;超声波处理法4.10。结论三种全血基因DNA提取方法操作简便、安全、快速,以改良高盐法提取效率最高。 展开更多
关键词 全血 基因组DNA 高盐 改良高盐 超声波处理法
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Rose Essential Oil Extraction from Fresh Petals Using Synergetic Microwave & Ultrasound Energy: Chemical Composition and Antioxidant Activity Assessment 被引量:2
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作者 Mariana Patrascu Marilena Radoiu 《Journal of Chemistry and Chemical Engineering》 2016年第3期136-142,共7页
The aim of the present work was to investigate the synergetic effect of microwave and ultrasound treatment on the production, chemical composition and antioxidant activity of rose essential oil. The rose essential oil... The aim of the present work was to investigate the synergetic effect of microwave and ultrasound treatment on the production, chemical composition and antioxidant activity of rose essential oil. The rose essential oil was extracted from fresh Rosa x damciscena Mill. petals by four methods, hydrodistillation, steam distillation, organic solvent extraction and ultrasounds followed by microwave hydrodistillation. The chemical composition of the extracts was analysed by GC-MS, and the antioxidant capacity by DPPH. It was found that both chemical composition and the antioxidant activity of the extracts depend on the extraction method. Overall it was found that microwaves coupled with ultrasonic treatment can be used effectively for the intensification of the extraction of monoterpenes and sesquiterpenes--fragrance bearing molecules--and equally, for increased antioxidant activity while using about 4 time shorter extraction time. The scale-up of the method was also evaluated. The results obtained in this research support the possible use of the US/MW method for the extraction of rose essential oil for the pharmaceutical and fragrance industry. 展开更多
关键词 2.45 GHz microwaves ULTRASOUNDS rose petals essential oil scale-up hydrodistillation.
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"Smart" ultrasonic pig design and its signal processing research
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作者 雷华明 阙沛文 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2008年第1期65-70,共6页
This paper introduces the design and development of intelligent ultrasonic "pig" used for seabed pipeline inspection. The data acquisition system (DAS) and the signal processing method are presented. The DAS was d... This paper introduces the design and development of intelligent ultrasonic "pig" used for seabed pipeline inspection. The data acquisition system (DAS) and the signal processing method are presented. The DAS was designed using a muhi-DSP based structure. The signal processing method adopted a well-enhanced split-spectrum processing (SSP) based on weighting algorithm according to statistical times, which was the statistical distribution of the discrete signals selected by the minimization algorithm. Furthermore, the data compressing method was discussed. It assures the mass data to be processed and compressed in real-time. The effectiveness of the method was proved by laboratory experiments. The research provides an excellent base for further development of the ultrasonic pig. 展开更多
关键词 intelligent ultrasonic pig seabed pipeline inspection split-spectrum processing muhi-DSP datacompressing
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