[Objective] This research aimed to explore the method to increase conversion rate of pollen-tub pathway in Chinese cabbage.[Method] Chinese cabbage varieties Yuqing and No.03 were used as materials for the selection o...[Objective] This research aimed to explore the method to increase conversion rate of pollen-tub pathway in Chinese cabbage.[Method] Chinese cabbage varieties Yuqing and No.03 were used as materials for the selection of germination buffer and parameters for ultrasonication.[Result] The optimal buffer for pollen germination of Chinese cabbage was 200 g/L sucrose ＋ 100 mg/L boric acid ＋ 200 mg/L calcium nitrate,the preferred ultrasonic processing power was 150 W,processing time was 5 s,interval time was 5 s and processing frequency was 8.Three T1-generation plants were obtained through selection with 200 mg/L kanamycin.[Conclusion] This research laid foundation for the further genetic transformation of Chinese cabbage.展开更多
基金Supported by Scientific and Technological Project in Shanxi Province(021034)Scientific Research Project of Shanxi Academy of Agri-cultural Sciences (YGX-01)~~
文摘[Objective] This research aimed to explore the method to increase conversion rate of pollen-tub pathway in Chinese cabbage.[Method] Chinese cabbage varieties Yuqing and No.03 were used as materials for the selection of germination buffer and parameters for ultrasonication.[Result] The optimal buffer for pollen germination of Chinese cabbage was 200 g/L sucrose ＋ 100 mg/L boric acid ＋ 200 mg/L calcium nitrate,the preferred ultrasonic processing power was 150 W,processing time was 5 s,interval time was 5 s and processing frequency was 8.Three T1-generation plants were obtained through selection with 200 mg/L kanamycin.[Conclusion] This research laid foundation for the further genetic transformation of Chinese cabbage.
