目的评价阜外改良心肌保存液(fuwai mod ified solution,FWM),HTK液(h istid ine-tryptophan-ketogluratesolution)和UW液(un iversity of w isconsin solution)对离体鼠心的保存效果。方法20只雄性W istar大鼠随机分为四组(Control组、...目的评价阜外改良心肌保存液(fuwai mod ified solution,FWM),HTK液(h istid ine-tryptophan-ketogluratesolution)和UW液(un iversity of w isconsin solution)对离体鼠心的保存效果。方法20只雄性W istar大鼠随机分为四组(Control组、FWM组、HTK组和UW组),每组5只。Control组切取鼠心后立即移至Langendorff装置灌注1h,其它三组分别用不同的保存液4℃下保存6 h,然后将离体鼠心移至Langendorff装置复灌1 h,测定复灌1h后的心肌含水量变化,并用透射电镜观察复灌1 h后心肌超微结构的改变。结果UW组心肌含水量高于Control、FWM和HTK组,而Control,FWM和HTK三组相比无显著统计学差异(P>0.05)。电镜结果显示,Control组和FWM组心肌超微结构基本正常,HTK组出现心肌超微结构改变,而UW组心肌超微结构改变严重。结论FWM液的心肌保存效果优于HTK和UW液。展开更多
Objective: To study the occurrence and development ofprimary syphilis from a morphological and structural aspect.Method: The ultrastructural pathological changes of syphiliticchancre were examined by transmission elec...Objective: To study the occurrence and development ofprimary syphilis from a morphological and structural aspect.Method: The ultrastructural pathological changes of syphiliticchancre were examined by transmission electron microscopy(TEM). Results: The pathological changes of syphilitic chancreoccur mainly in the dermal layer, manifested as partialthinning or lysis of the capillary basal membrane,proliferation of capillary endothelial cells, thickening of somecollagen fibers and rupture of collagen fibers surrounding Tpallidum, structural disruption of the axons of terminal nerves,disarrangement of the sheath of the myelinated nerve fiberswith separation of laminae and the attachment of T pallidumon its outer membrane, appearance of slightly swollen Tpallidum in the plasma of the capillary endothelial cells andfibroblasts, and infiltration of neutrophils, macrophages andplasma cells containing T pallidum and its debris frequentlylined by a clear sheath. Conclusion: The pathogenesis and injury of mechanism inprimary syphilis can be explained morphologically by itscharacteristic ultrastructural pathological changes.展开更多
文摘目的评价阜外改良心肌保存液(fuwai mod ified solution,FWM),HTK液(h istid ine-tryptophan-ketogluratesolution)和UW液(un iversity of w isconsin solution)对离体鼠心的保存效果。方法20只雄性W istar大鼠随机分为四组(Control组、FWM组、HTK组和UW组),每组5只。Control组切取鼠心后立即移至Langendorff装置灌注1h,其它三组分别用不同的保存液4℃下保存6 h,然后将离体鼠心移至Langendorff装置复灌1 h,测定复灌1h后的心肌含水量变化,并用透射电镜观察复灌1 h后心肌超微结构的改变。结果UW组心肌含水量高于Control、FWM和HTK组,而Control,FWM和HTK三组相比无显著统计学差异(P>0.05)。电镜结果显示,Control组和FWM组心肌超微结构基本正常,HTK组出现心肌超微结构改变,而UW组心肌超微结构改变严重。结论FWM液的心肌保存效果优于HTK和UW液。
文摘Objective: To study the occurrence and development ofprimary syphilis from a morphological and structural aspect.Method: The ultrastructural pathological changes of syphiliticchancre were examined by transmission electron microscopy(TEM). Results: The pathological changes of syphilitic chancreoccur mainly in the dermal layer, manifested as partialthinning or lysis of the capillary basal membrane,proliferation of capillary endothelial cells, thickening of somecollagen fibers and rupture of collagen fibers surrounding Tpallidum, structural disruption of the axons of terminal nerves,disarrangement of the sheath of the myelinated nerve fiberswith separation of laminae and the attachment of T pallidumon its outer membrane, appearance of slightly swollen Tpallidum in the plasma of the capillary endothelial cells andfibroblasts, and infiltration of neutrophils, macrophages andplasma cells containing T pallidum and its debris frequentlylined by a clear sheath. Conclusion: The pathogenesis and injury of mechanism inprimary syphilis can be explained morphologically by itscharacteristic ultrastructural pathological changes.
