自体全厚皮片移植后表皮—黑色素单位超微结构动态变化

目的 探讨自体全厚皮片移植后色素沉着的成因。方法 应用超微结构细胞化学技术观察自体全厚皮片移植后表皮—黑色素单位 (EpidermalMelaninUnit,EMU)的超微结构动态变化 ,并借助电子计算机辅助图像分析技术 ,对黑色素细胞及角朊细胞内的黑色素小体形态学及分布形式进行分析。结果 术后 48h内表皮黑色素单位处于崩解状态 ;术后 72h后 ,表皮黑色素细胞 (Mel anocyte ,MC)及角朊细胞 (Kerationcyte,KC)功能及协同作用逐渐恢复并超过术前水平 ;1个月后 ,KC内 ,尤其是棘层及颗粒层KC内出现大量、呈单个分布的MS。结论 EMU内KC MC协同功能增强及从基底层到颗粒层的KC内出现大量大体积、呈单个形式分布的MS 。

THE SECRETORY STIGMAS IN POPULUS: DEVELOPMENT, CYTOCHEMISTRY, ULTRASTRUCTURE AND THEIR RELATION TO INTERSECTIONAL INCOMPATIBILITY *

The stigmas of five species, Populus euphratica Oliv., P. alba L., P. simonii Carr., P. lasiocarpa Oliv. and P. nigra L. have been studied. Scanning electron microscopy re veals that exudates are present in the intercellular spaces, in the clefts between the multicel lular papillae and on the receptive surface. Release and movement of exudates can be visual ized when the fresh stigmas are stained with sudan Ⅲ and auramine O. Paraffin and semi thin resin sections of stigmas after glutaraldehyde osmium fixation evidence the lipidic nature of the exudates. Transmission electron microscopy reveals the glandular features of the stigmatic papillae cells, such as abundance of rough and smooth endoplasmic reticulum, polyribosomes, and well developed dictyosomes with secretory vesicles. Pellicle and epicuticular lamellate layers which have been considered as typical features of the dry type stigmas are also present in the species where stigmas appear extremely wet. It is concluded that stigmas in all of the five species are secretory at the receptive stage. Well developed generative and sperm cells were observed in the pollen tubes penetrating through the deep layers of the stigmatic tissue in the reciprocal crosses between P. euphratica and P. simonii, which indicated that there is no significant barrier in the stigma.

海水型呼吸窘迫综合征兔肾皮质酶活性定位和图像分析

本文采用超微结构细胞化学和图像分析技术,对兔海水型呼吸窘迫综合征(SW-RDS)肾皮质酶活性的变化进行了观察。SW-RDS组肾皮质碱性磷酸酶、5′-核苷酸酶、对-硝基苯磷酸酶和细胞色素氧化酶活性明显下降,而胞嘧啶单核苷酸酶活性明显增强。这表明肾皮质一些酶活性的改变是兔SW-RDS发病机理之一。