光量子化处理前后的新鲜库血LPO、SOD含量变化的探讨

光量子化处理前后的新鲜库血ＬＰＯ、ＳＯＤ含量变化的探讨０３２２００山西省汾阳医院高志华，牛建华，张俊英，贾春生，昌燕宗光量子化后的新鲜库血治疗某些疾患，已显示出独特的疗效。但对处理前后新鲜库血成分变化的研究尚未见报道。为探求光量子后新鲜库血的治疗机理...

Effect of Polyamine Priming on Chilling Tolerance of Lolium perenne during Seed Imbibition

[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.

Enzyme responses and lipid peroxidation in gills and hepatopancreas of clam Mactra vereformis, following cadmium exposure

To assess the toxicity of heavy metal pollution to marine intertidal shellfish, enzymatic responses and lipid peroxidation were investigated in the clam Mactra vereformis exposed to cadmium under laboratory conditions. Three antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx), two immune defense enzymes (acid phosphatase, ACP; alkaline phosphatase, ALP), and one lipid peroxidation product (malondialdehyde, MDA) were measured in the gills and the hepatopancreas of the clam exposed to 0, 25, 75, and 125 μg/L cadmium for 0, 1, 3, 5, and 7 d. The results show that the concentrations of antioxidant enzymes in the organs soared to a peak value on the first day and then decreased afterwards in most cases. CAT and GPx activities in the hepatopancreas were higher than in the gills, but the SOD activity was lower in the hepatopancreas. ACP activity was unchanged until Day 3 in the hepatopancreas and until Day 5 in gills, when it began to increase. ALP activity showed no significant relationship with Cd treatment. MDA concentrations increased in the two tissues after Cd exposure, peaked on Day 3 in gills, and on Day 5 in hepatopancreas. These observations show that changes in the activities of antioxidant enzymes and ACP reflect the time course of oxidative stress in the clam caused by Cd, and could be used as potential biomarkers for ecotoxicological bioassays of heavy metals.

Promotive Effects of Alginate-Derived Oligosaccharides on the Inducing Drought Resistance of Tomato

In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO (0.05%, 0.10%, 0.20%, 0.30% and 0.50%) after drought stress was simulated by exposing the roots to 0.6 molL-1 PEG-6000 solution for 6 h. Changes in biomass, electrolyte leakage and malondialdehyde (MDA), free proline, total soluble sugars (TSS) and abscisic acid (ABA), the enzyme activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.

Oxidative stress responses of submerged macrophyte Vallisneria asiatica to different concentrations of cyanobacteria

In a 10-day aquarium experiment, this investigation examines macrophyte restoration in eutrophic Lake Taihu, the physiological effects of different plant biomass levels and of increasing natural cyanobacterial concentrations on a submerged macrophyte, Vallisneria asiatica. Cyanobacterial stress suppressed the superoxide dismutase （SOD） activity of the plant＇s leaves and induced the catalase （CAT） and peroxidase （POD） activities of its roots. The soluble protein content in V. asiatica decreased with an increase in natural cyanobacterial concentrations, whereas the malonaldehyde （MDA） increased significantly at chlorophyll a （Chl a） concentrations of 222 and 262 μg/L in water. V. asiatica adapted to the stress caused by cyanobacterial concentrations by adjusting its antioxidant defense system to remove the excessive reactive oxygen species when the algal Chl a concentration was 〉109 μg/L. Additionally, high biomass of V. asiatica （2 222 g FW/m^2） can inhibit the reproduction of cyanobacteria more significantly than low biomass （1 111 g FW/m^2）. High biomass of V. asiatica increased the oxidative stress in an individual plant when the initial Chl a concentration in the water reached 222 and 262 μg/L, as expressed by the increased MDA in leaves, compared with low biomass of K asiatica. This provides a basis for controlling cyanobacterial concentrations and V. asiatica biomass for the recovery of V. asiatica in eutrophic Lake Taihu.

Oxidants and Anti-Oxidants in Turbot Seminal Plasma and Their Effects on Sperm Quality

In this research, the concentration and activity of oxidants and anti-oxidants in turbot semen, and their effects on sperm quality were studied. The results showed that superoxide dismutase(SOD), catalase, glutathione reductase(GR), uric acid, vitamin E(VE) and vitamin C(VC) were more abundant in seminal plasma than in spermatozoa. The variation for each of them was specific. In seminal plasma, the activity of SOD and GR increased from November 15, November 30 to December 15, and then decreased on December 30. The concentrations of both VC and uric acid decreased during the first 3 sampling times and increased on December 30. The oxidants in seminal plasma accumulated to the highest on December 30. Lactic acid(LA) and ATP levels decreased to the lowest on December 30. The correlation analysis showed that GR had the significant positive relevance to sperm motility and VSL/VCL, while ·OH had negative relevance to them.

Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis

AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time points.In a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs group.Serum AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into rats.In both studies,the protective effect of MSCs was evaluated.RESULTS:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time point.In vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP group.Serum SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 h.Intestinal mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting MSCs.CONCLUSION:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic inflammation in rats with SAP.

Impact of agricultural activities-motor vehicles and e-waste on sediment characteristics of the Serin River, Malaysia

Sediment is a sink for organic materials, nutrients and heavy metals and sediment condition affects the overlying water Though Serin River is a source of drinking water, agricultural and waste disposal activities in the watershed may impact the sediment of the river. Therefore, the objective of this study was to investigate the organic matter, nutrients and heavy metals in the sediment of the Serin River. Five stations were selected for sediment sample collection. Results of the study show that organic matter （OM） ranged from 0.7% to 5.9%, TP was 100 -366 （mg/kg）, TAN was 16-141 （mg/kg）, TON was 550-3019（mg/kg）, and TKN was 566-3160 （mg/kg）. Sediment oxygen demand （SOD20） ranged from 5.6 to 14.2 （g O2/m^2/d）. Among the five stations OM, TKN, and SOD of the sediment were second highest at the two stations downstream of animal （fish, chicken and pig） farming. TP and TAN were the second highest at the station downstream of fish farming and third highest at the station downstream of pig farming. Zn, Cu and Cd ranged from 132-357 （mg/kg）, 26.7-96.7 （mg/kg） and 4.0-19.3（mg/kg）. According to USEPA guideline, sediments at four stations were heavily polluted with Zn, Cu and Cd whereas the station that was farthest upstream, near a village, was slightly polluted with Zn and Cu. This study indicates that animal farming has an impact on the organic matter content, nutrients （N and P）, oxygen demand and heavy metals of the sediment. Motor vehicles and discarded e-waste also contributed to the heavy metals in the sediment. Farm effluents and storm water should be treated and e-waste recycled to protect the water quality of the river for its designated use.

Targeting of AUF1 to vascular endothelial cells as a novel anti-aging therapy

Background Inhibition of aging of vascular endothelial cells （VECs） may delay aging and prolong life. The goal of this study was to prepare anti-CD31 monoclonal antibody conjugated PEG-modified liposomes containing the AU-rich region connecting factor 1 （AUF1） gene （CD31-PILs-AUF1） and to explore the effects of targeting CD31-PILs-AUF1 to aging VECs. Methods The mean particle sizes of various PEGylated immunoliposomes （PILs） were measured using a Zetasizer Nano ZS. Gel retardation assay was used to confirm whether PILs had encapsulated the AUF1 plasmid successfully. Fluorescence microscopy and flow cytometry were used to quantify binding of CD31-PILs-AUF1 to target cells. Flow cytometry was also used to analyze the cell cycles of aging bEnd3 cells treated with CD31-PILs-AUF1. We also developed an aging mouse model by treating mice with D-galactose. Enzyme-linked immunosorbent assay （ELISA） was used to evaluate the levels of interleuldn-6 （IL-6） and tumor necrosis factor-or （TNF-ct）. The malondialdehyde （MDA） and the superoxide dismutase （SOD） levels were detected by commercial kits. Hematoxylin-eosin （HE） staining was used to determine whether treatment with CD31-PILs-AUF 1 was toxic to the mice. Results CD31-PILs-AUF 1 specifically could targeted bEnd3 VECs and increased the percentage of cells in the S and G2/M phases of aging bEnd3 cells. ELISA showed that content of the IL-6 and TNF-ct decreased in CD31-PILs-AUF1 group. The level of SOD increased, whereas MDA decreased in the CD31-PILs-AUF1 group. Additionally, CD31-PILs-AUF 1 was not toxic to the mice. Conclusion CD31-PILs-AUF 1 targets VECs and may delay their senescence.

Antioxidant System of the Intertidal Mollusk Littorina kurila in Its Natural Habitat

Based on the theory of oxidative stress, it is believed that the induction of antioxidant components of the cellular defense system reflects the adaptive compensatory response of an organism aimed at overcoming stressful conditions. Therefore, this work concentrated on investigating the effect of the low and high tides on the antioxidant system of L. （Littorina） kurila and also compared the antioxidant parameters of two species of gastropods： intertidal--L, kurila and subtidal--L, squalida. The following parameters were evaluated in the hepatopancreas of mollusks： the activity of the antioxidant enzymes SOD （superoxide dismutase）, CAT （catalase）, and GR （glutathione reductase）, the production of ROS （reactive oxygen species）, and the levels of GSH （glutathione） and the lipid peroxidation product-MDA （malondialdehyde）. The results of this work indicate that the state of the antioxidant system of L. kurila reflected the adaptive capacity of the species in the changing environmental conditions.

Physiological Response of Hydrilla verticillata （I.f.） Royle Exposed to Cadmium Stress

Hydrilla verticillata （I.f.） Royle twigs were exposed to CdCI2 （50, 100, 200 and 500 BM） under continuous light for 48 hrs and the physiological parameters like photosynthetic pigment （chlorophylls a, b and carotenoids） contents, activities of antioxidative enzymes like superoxide dismutase （SOD）, catalase （CAT） and peroxidase （POX） along with lipid peroxidation level were determined. With respect to increase in Cd concentration in the medium and exposure duration, decrease in pigment contents, and decrease in the activities of SOD, CAT and POX were found. The increased levels of lipid peroxidation indicated the prevalence of oxidative stress situation in the tissues which might be one of the reasons behind Cd induced toxicity in Hydrilla verticillata. Since there was significant decrease in the activities of key antioxidative ezymes, the study suggests that Hydrilla verticillata may not be effective for phytoremediation of cadmium in these concentration ranges.

Simulation for Water Quality Management in Inohana Lake Estuary, Japan

The Inohana Lake is a branch lake of the Hamana Lake. The Inohana Lake is an estuary rather than a brackish lake, and has suffered environmental problems such as eutrophication and bottom hypoxic water. In this study, the coupled hydrodynamic and ecological models （eco-hydrodynamic model） were used to construct the strategy for preventing the bottom hypoxic water and improving or recovering the water quality in the lake. Using the model input obtained from the summertime data over 1998-2002, the summer-average flow field and oxygen concentration and budget of the standard run were calculated. Remedial measures used in this study are divided into two parts： the biogeochemical and physical changes in the present situation. For the remedial measures including the biogeochemical changes in the present situation, the simulations considering the reductions of the nutrient inputs from the river, main lake （land） and bottom sediment, and the sediment oxygen demand （SOD） were carried out. For the remedial measures including the physical changes, the 50 and 100 m extensions of the inlet width were considered in the model runs. These simulated results were compared in terms of changes in the dissolved oxygen （DO） concentration and oxygen budget in the bottom layer in the Inohana Lake. There was no significant change in the DO concentration and oxygen stock in the simulations for the reduction of the nutrient inputs from the land and bottom sediment, however increases in those in the simulations for the reduction of SOD. When SOD was reduced by 50%, the bottom DO concentration increased by approximately 2 mg/L and the oxygen stock in the bottom layer increased by 47% comparing the present situation （the standard run） of the lake. The simulation results for inlet width extension showed that the extension of width makes the DO concentration and oxygen stock lower. The remedial measures for the sediment control were proposed to prevent the bottom hypoxia and manage the water quality.

Physiological traits of rice seedlings in response to inorganic arsenic

The aim of our study was to better understand the different responses of rice seedling to different species of inorganic arsenic As203 （As（Ill）） and Na2HAsO4 （As（V））. Our results indicate that the biomass of rice seedling decreased as arsenic concentration increased, with the decrease being more significant at higher arsenic concentrations. In addition, the analysis of superoxide dimutase （SOD）, peroxidase （POD）, and catalase （CAT） in rice roots and leaves showed that the activity of these three enzymes significantly decreased in rice tissues, especially in rice roots, as arsenic concentration was increased,. Further, the uptake and utilization efficiencies of N, P, and K were found to decrease as arsenic concentration was increased. However, the uptake and utilization efficiencies of P and K were mainly affected by As（IlI）, whereas those of N were mainly affected by As（V）. Inductively coupled plasma-mass spectrometry （ICP-MS） was used to assay arsenic accumulation in rice tissues; the results indicate that the arsenic content in rice tissues was enhanced when arsenic concentration was increased, especially in rice roots after arsenic treatment.