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镉污染土壤的植物修复研究进展与展望 被引量:25
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作者 苏慧 魏树和 周启星 《世界科技研究与发展》 CSCD 2013年第3期315-319,343,共6页
近年来,重金属镉(Cd)及其复合污染问题越来越受到人们的关注。Cd污染土壤的植物修复技术因其具有治理成本的低廉性、环境美学的兼容性和治理过程的原位性等优势,随之成为具有广泛应用前景的技术。本文概述了Cd超积累植物的筛选及耐性机... 近年来,重金属镉(Cd)及其复合污染问题越来越受到人们的关注。Cd污染土壤的植物修复技术因其具有治理成本的低廉性、环境美学的兼容性和治理过程的原位性等优势,随之成为具有广泛应用前景的技术。本文概述了Cd超积累植物的筛选及耐性机理与修复潜力的评价等方面的国内外进展,总结了Cd-重金属复合污染土壤、Cd-有机物复合污染土壤的植物修复相关的重要工作,着重评述了Cd复合污染土壤的化学强化、农业生态强化及其它方法的研发现状,展望了这一领域今后的重点研究内容和重要发展方向。 展开更多
关键词 污染土壤 复合污染 植物修复 超积累体
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Cellular Tolerance, Accumulation and Distribution of Cadmium in Leaves of Hyperaccumulator Picris divaricata 被引量:2
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作者 HU Peng-Jie GAN Yuan-Yuan +4 位作者 TANG Ye-Tao ZHANG Quan-Fang JIANG Dan YAO Nan QIU Rong-Liang 《Pedosphere》 SCIE CAS CSCD 2012年第4期497-507,共11页
Knowledge of cellular metal homeostasis will provide a better understanding of the mechanisms involved in metal tolerance and hyperaccumulation in metal-hyperaccumulating plants. Energy dispersive X-ray spectrometry ... Knowledge of cellular metal homeostasis will provide a better understanding of the mechanisms involved in metal tolerance and hyperaccumulation in metal-hyperaccumulating plants. Energy dispersive X-ray spectrometry (EDS) was used to determine the localization of cadmium (Cd) in leaves of the Zn/Cd hyperaccumulator Picris divaricata which had a shoot Cd concentration of 565 mg kg-1 after 2 weeks of growth in solution culture supplying 10μ tmol L^-1 CdCl2. The results indicated that Cd was distributed mainly in the trichomes, upper and lower epidermis and bundle sheath cells, with a relatively low level of Cd in mesophyll cells. Mesophyll protoplasts isolated from leaves remained viable after 24 h exposure to CdCl2 at a concentration up to 1 mmol L^-1, indicating their high tolerance to Cd. The intracellular Cd was visualized by staining with Leadmium Green dye, a cellular permeable Cd fluorescence probe. The results showed that the majority of protoplasts (〉 82%) did not accumulate Cd, with only a minority (〈 18%) showing Cd accumulation. In the Cd-accumulating protoplasts, Cd accumulation was depressed by the addition of Fe^2+, Mn^2+ and the metabolic inhibitor carbonyl cyanide m-chlorophenylhydrazone (CCCP), but not by Ca^2+ or Zn^2+. Furthermore, the entire process of Cd uptake from external solution into the cytoplasm and subsequent sequestration into vacuoles was successfully recorded by confocal images. These results suggested that reduced cellular Cd accumulation and efficient Cd vacuolar sequestration in mesophyll cells might be responsible for cellular Cd tolerance and distribution in the leaves of P. divaricata. 展开更多
关键词 confocal images fluorescence probe MESOPHYLL PROTOPLAST VACUOLAR
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