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巨噬细胞趋化检测方法的建立及皮质酮对其趋化功能的影响 被引量:1
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作者 钟河江 王海燕 +5 位作者 刘庆 蒋建新 杨策 严军 黄苏娜 杨天德 《重庆医学》 CAS CSCD 北大核心 2009年第15期1876-1878,1881,共4页
目的建立改良Boyden趋化小室(Boyden chamber)法检测巨噬细胞趋化功能的方法,探讨皮质酮对大鼠腹腔巨噬细胞趋化功能的影响。方法分离成年Sprague-Dawley(SD)大鼠腹腔巨噬细胞。应用48孔Boyden chamber趋化板检测趋化功能。下室加入27μ... 目的建立改良Boyden趋化小室(Boyden chamber)法检测巨噬细胞趋化功能的方法,探讨皮质酮对大鼠腹腔巨噬细胞趋化功能的影响。方法分离成年Sprague-Dawley(SD)大鼠腹腔巨噬细胞。应用48孔Boyden chamber趋化板检测趋化功能。下室加入27μL的10nM FMLP作为趋化剂或含1%牛血清清蛋白的RPMI-1640作为阴性对照,将50μL不同细胞密度的大鼠腹腔巨噬细胞悬液(3、6、12×106/mL)置于上室以确定最适细胞密度,将趋化装置置于5%CO2、37℃细胞培养箱内孵育2、3h以确定最适孵育时间。同时,以0、10、501、000ng/mL皮质酮处理大鼠腹腔巨噬细胞,应用Boyden chamber法检测巨噬细胞趋化功能。结果巨噬细胞对10nM FMLP趋化剂呈细胞密度依赖性增加,上室巨噬细胞密度为6×106/mL时为最适细胞密度,并且最适孵育时间为3h。低浓度皮质酮(10、50ng/mL)处理大鼠腹腔巨噬细胞其趋化指数显著高于对照组(P<0.01),而高浓度皮质酮(1 000ng/mL)处理后巨噬细胞趋化指数与对照组相比差异不明显。结论成功建立了改良Boyden chamber法检测巨噬细胞趋化功能方法,皮质酮可呈剂量依赖性地影响巨噬细胞趋化功能,在无免疫刺激状态下,低浓度皮质酮能增强巨噬细胞趋化功能。 展开更多
关键词 趋化检测 巨噬细胞 皮质酮
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Change Point Detection and Trend Analysis for Time Series
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作者 Hong Zhang Stephen Jeffrey John Carter 《Chinese Journal of Chemical Physics》 SCIE EI CAS CSCD 2022年第2期399-406,I0004,共9页
Trend analysis and change point detection in a time series are frequent analysis tools.Change point detection is the identification of abrupt variation in the process behaviour due to natural or artificial changes,whe... Trend analysis and change point detection in a time series are frequent analysis tools.Change point detection is the identification of abrupt variation in the process behaviour due to natural or artificial changes,whereas trend can be defined as estimation of gradual departure from past norms.We analyze the time series data in the presence of trend,using Cox-Stuart methods together with the change point algorithms.We applied the methods to the nearsurface wind speed time series for Australia as an example.The trends in near-surface wind speeds for Australia have been investigated based upon our newly developed wind speed datasets,which were constructed by blending observational data collected at various heights using local surface roughness information.The trend in wind speed at 10 m is generally increasing while at 2 m it tends to be decreasing.Significance testing,change point analysis and manual inspection of records indicate several factors may be contributing to the discrepancy,such as systematic biases accompanying instrument changes,random data errors(e.g.accumulation day error)and data sampling issues.Homogenization technique and multiple-period trend analysis based upon change point detections have thus been employed to clarify the source of the inconsistencies in wind speed trends. 展开更多
关键词 Time series Change point detection Trend analysis Wind speed HOMOGENIZATION
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