足三里灸配合推拿治疗64例膝骨关节炎患者疗效观察

目的探究足三里灸配合推拿治疗膝骨关节炎患者临床疗效。方法选取我院2016年5月~2018年5月膝骨关节炎患者128例,随机数字表法分为对照组和观察组各64例。对照组给予足三里灸治疗,观察组给予足三里灸配合推拿治疗。对比两组症状体征(肿胀、僵硬、关节功能障碍)消失时间、疗效及治疗前后膝关节功能评分表(百分法)评分。结果观察组治疗总有效率为93.75%,高于对照组的76.56%,差异有统计学意义(P<0.05);治疗后观察组关节屈曲度、上下楼梯、关节稳定度、疼痛、绞锁分值高于对照组,差异有统计学意义(P<0.05);观察组肿胀、僵硬、关节功能障碍消失时间短于对照组,差异有统计学意义(P<0.05)。结论足三里灸配合推拿治疗膝骨关节炎患者,可显著改善症状、体征,恢复膝关节功能,疗效确切。

足三里灸配合推拿治疗64例膝骨关节炎患者疗效观察

目的探究足三里灸配合推拿治疗64例膝骨关节炎患者疗效观察。方法选取2018年9月-2019年11月收治的膝骨关节炎患者64例,依据认知治疗方式的不同,随机分为对照组(足三里灸治疗)与观察组(足三里灸配合推拿治疗),每组32例。比较两组临床疗效。结果观察组临床疗效总有效率(93.75%)明显高于对照组(75.00%)(P<0.05)。结论经足三里灸配合推拿治疗,可显著改善膝骨关节炎患者病症,提高临床治疗效果。

温和灸足三里对胃癌患者免疫功能的影响

目的探讨温和灸足三里对胃癌患者免疫功能的作用。方法选取2010年10月—2012年8月就诊于上海中医药大学附属龙华医院的胃癌患者64例。随机分为治疗组和对照组各32例,两组患者药物治疗基本一致,均施行"辩证"施护,治疗组在对照组基础上增加温和灸治疗。6个月后检测外周血中实验室免疫功能指标情况和中医脾气虚症状积分改善情况。结果治疗组CD3+T细胞、CD4+T细胞和NK细胞均较对照组增加(P<0.05);两组患者治疗前后自身的脾气虚症状积分均有所改善(P<0.01),两组比较差异具有统计学意义(P<0.05)。结论温和灸足三里能提高胃癌患者体内调节系统的调节品质,增强胃癌患者的机体免疫力。

足三里雷火灸联合耳穴贴压治疗原发性骨质疏松症的疗效观察

目的探讨足三里雷火灸联合耳穴贴压治疗原发性骨质疏松症的作用效果。方法将100例原发性骨质疏松症患者根据自愿参与原则随机分为治疗组和对照组,每组各50例,2组均予口服阿法骨化醇胶囊+碳酸钙D3片治疗,治疗组在用药基础上采用足三里雷火灸联合耳穴贴压治疗,对照组不予其他治疗。2组均以1个月为一个疗程,观察3个月,对比2组治疗前后患者骨质疏松主要症状、测定VAS疼痛评分和骨密度(BMD)水平。结果治疗组总疗效明显优于对照组(P<0.05)。结论足三里雷火灸联合耳穴贴压治疗原发性骨质疏松症,能有效改善患者的腰酸背痛、腰膝酸软、下肢乏力等症状,且不增加不良反应,安全性高。

足三里温和灸配合神阙静振推拿法治疗儿童脾虚气弱型功能性便秘临床观察

目的:观察足三里温和灸配合神阙静振推拿法治疗脾虚气弱型儿童功能性便秘的临床疗效。方法:选择120例脾虚气弱型功能性便秘患儿,随机分为观察组和对照组,每组60例。观察组予足三里温和灸配合神阙静振推拿法治疗,对照组予双歧杆菌乳杆菌三联活菌片口服治疗。观察两组患儿临床疗效及安全性。结果:观察组总有效率[81.03%(47/58)]与对照组[84.48%(49/58)]比较,差异无统计学意义(P>0.05);在每周排便次数、每次排便时间等方面观察组优于对照组(P<0.05);治疗结束1个月后随访,观察组总有效率[77.59%(45/58)]与对照组[65.51%(38/58)]比较,差异有统计学意义(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:足三里温和灸配合神阙静振推拿法治疗脾虚气弱型儿童功能性便秘疗效显著,其治疗效果优于双歧杆菌乳杆菌三联活菌片口服治疗,复发率低,且安全性较高。

CLINICAL APPLICATION OF NEIGUAN (内关 PC 6) IN THE TREATMENT OF DISORDERS OF THE DIGESTIVE SYSTEM——THE RELATED RESEARCH PROGRESS

In the present paper, the authors review recent situations of clinical application of Neiguan (PC 6内关), etc. in the treatment of disorders of the digestive system and its related research progresses. In clinical practice, Neiguan (PC 6) is usually selected as one of the main acupoints to treat gastrointestinal reactions induced by ① nausea and vomiting, ② hiccup, ③ gastrointestinal dyskinesia, ④ gastritis, and ⑤ intra-gastric examination, cesarean section, etc. Concerning experimental studies, the effects of Neiguan (PC 6), etc. on ① gastric electrical activities, ② gastrointestinal motion, ③ gastrointestinal hormone secretion, and ④ gastric mucosal structure were observed. Results indicated that Neiguan (PC 6) is an important and effective acupoint for treatment of gastrointestinal disorders in clinic. Stimulation of Neiguan (PC 6) induced favorable regulation of both the peripheral nervous system and central nervous system, and changes of the gastrointestinal hormone secretion may contribute to its effects in treating various disorders.

Effect of purulent moxibustion on Zúsānlǐ(足三里ST 36) point on the clinical symptoms of patients with malignant tumor

Objective To discuss the effect of purulent moxibustion on Zusanli （足三里 ST 36） point on the clinical symptoms and life quality of patients with malignant tumor. Methods Sixty patients with malignant tumor were divided into an observation group and a control group with 30 cases in each group. The observation group was treated with purulent moxibustion on Zusanli （足三里 ST 36） in combination with herbal medicine; the control group was only treated with herbal medicine. After 80 days, the changes of the clinical symptoms and KPS of the two groups before and after the treatment were observed. Results The effective rates for the improvement of lassitude, poor appetite and insomnia in the observation group were 72.4% （21/29） ,25.0% （15/20） ,71.4% （15/21）, and those in the control group were 42.9%（12/28） ,55.6% （10/18） ,42.9% （6/14）, and there was no statistical significance of difference between the two groups（P〉0.05）. KPS has statistical significance in the two groups after the treatment （P〈0.01）,and the observation group was higher than the control group. Conclusion Purulent moxibustion on the point of Zusanli （足三里 ST 36） has certain effect to improve the clinical symptoms of patients with malignant tumor, and raise KPS and upgrade the life quality of patients.

Clinical observation on the correlation between moxibustion sensation and distance of moxa stick

Objective： To explore the correlation between moxibustion sensation and distance of moxa stick and provide reference for clinical practice.Methods： A total of 16 healthy volunteers aged 18-35 years old in college were recruited and given mild moxibustion at Shousanli（LI 10）, Zusanli（ST 36）, Shenshu（BL 23） and Tianshu（ST 25） with moxa stick, and the occurrence and frequency of moxibustion sensation were recorded at distances of 5 cm, 4 cm, 3 cm and 2 cm. Mild moxibustion scale was used to count the score.Results： Warm was the main moxibustion sensation, burning pain and soreness decreased with the rise of distance; for the same acupoint, score of mild moxibustion scale increased with the decrease of distance; score ranged between 5.5 and 6.5at distance 3 cm, which was the most comfortable distance for volunteers.Conclusion： The distance of 3 cm is the most comfortable distance in mild moxibustion.

Effects of different doses of ginger-partitioned moxibustion on trefoil factor 1,mucin 5AC and epidermal growth factor receptor in rats with spleen deficiency syndrome

To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 （TFF1） and mucin 5AC （MUCSAC） levels, as well as the expression of epidermal growth factor receptor （EGFR） in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome. Methods： Seventy-five SPF grade Sprague-Dawley （SO） rats were randomly divided into a blank control group （group A）, a model group （group B）, a 3 moxa-cone ginger-partitioned moxibustion group （group C1）, a 6 moxa-cone ginger-partitioned moxibustion group （group C2） and a 9 moxa-cone ginger-partitioned moxibustion group （group C3） using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 ~C 200% concentrated Da Huang （Radix et RhizomaRhei） to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli （ST 36）and Zhongwan （CV 12） respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay （ELISA）. The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry. Results： After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased （all P〈0.01）; compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased （all P〈0.01）. Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased （all P〈0.01）, however, there was no significant difference between group C2 and C3 （all P〉0.05）. The mechanism may be related to the increase of serum TFF2 and MUC5AC levels and activation of EGFR protein. Conclusion： Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone Ringer-Dartitioned moxibustion groups.

Mechanism study of the transmission of moxibustion heat in human acupoint tissues

Objective: To discuss the topical action characteristics of the biological transmission of moxibustion heat via temperature collection and numerical modeling. Methods: Temperature of moxibustion was measured at multiple points at a distance of 3 cm to obtain the moxibustion temperature field nephograms by the high-accuracy temperature measure array. Finite element analysis was used to imitate the three-dimensional dynamic distribution of temperature in acupoint tissues. Results: Through numerical analysis, the one-dimensional, two-dimensional and three-dimensional distributions of temperature in human acupoint tissues at 5 min of moxibustion were established. The result showed that moxibustion heat mainly tran smitted from the surface of the tissue to the internal, and the in flue nee of moxibusti on heat decreased with the depth of the tissue. The analysis of the nephograms of acupoint tissue temperature at 5,10, 15 and 20 min of moxibustion showed that with the in crease of the moxibusti on time, the temperature in acupoint tissues consta ntly rose, and the transmission depth of moxibustion heat also further expanded inside acupoint. Conclusion: By establishing the three-dimensional dynamic model of heat transmission inside acupoint tissues with the biological parameters of human tissues and the temperature values obtained, this study used finite element analysis software ANSYS 14.0 and discovered the rules in the transmission of heat in body tissues during moxibustion, and the features in moxibustion heat transmission (from the proximal to the distant) and heat penetration (from the surface to the intern al). This study provides theoretical and experime ntal support for the application of moxibusti on in clinical practice.

Low-frequency fluctuation amplitude analysis of resting-state fMRI for functional brain response differences between acupuncture and moxibustion at Zusanli(ST 36) in patient with functional dyspepsia

Objective： To compare and analyze functional brain response characteristics by applying acupuncture or moxibustion to Zusanli（ST 36） in patients with functional dyspepsia（FD） and investigate the differences of central action mechanism resulting from acupuncture or moxibustion.Methods： A total of eligible 24 FD cases were divided into two blood-oxygen-level dependent（BOLD） sequences for functional magnetic resonance imaging（fMRI） scan. The amplitude of low frequency fluctuation（ALFF） analyses were conducted on the data of location phase, structure phase, resting state before acupuncture/moxibustion, working state during acupuncture/moxibustion and resting state after acupuncture/moxibustion using Data Processing Assistant for Resting-State fMRI（DPARSF） software.Results： Acupuncture and moxibustion produced significant differences in functional brain response. The working state during acupuncture/moxibustion mainly decreased ALFF values in the right supramarginal gyrus, right superior parietal lobule, right frontal gyrus, upper right occipital lobe, right precuneus and right cingulate gyrus. At the same time, it increased ALFF values in the left cerebellum, right caudate nucleus, right cerebellum and left inferior gyrus. The differences during the resting state after acupuncture/moxibustion were significantly smaller than the working state in intensity and size. It mainly resulted in decrease in ALFF values in the right postcentral gyrus and right supramarginal gyrus and increase in ALFF values in the left precuneus, orbital part of inferior frontal gyrus and right cerebellar peduncles.Conclusion： Needling and moxibustion at Zusanli（ST 36） can produce significant differences in immediate functional brain response.

Effect of herbal cake-partitioned moxibustion on MEK1/2 and ERK1/2 expressions of gastric tissues in rats with spleen deficiency syndrome

Objective： To observe the effect of herbal cake-partitioned moxibustion on the expressions of mitogen-activated protein kinase （MEK：1/2） and extracellular regulatory protein kinase （ERK：1/2） in gastric tissues of rats with spleen deficiency syndrome, and to explore the possible mechanisms of herbal cake-partitioned moxibustion in treating spleen deficiency syndrome. Methods： Sixty Sprague-Dawley （SD） rats were randomly divided into a blank control group （group A）, a model group （group B）, a ranitidine group （group C）, and a herbal cake-partitioned moxibustion group （group D） by random digit, 15 rats in each group. Rat models of spleen deficiency syndrome were made by intragastric administration of 4℃ 200% concentrated Da Huang （Radix et Rhizoma Rhei）. After successful modeling, the rats in group C were treated with 25 mg/（kg.bw） ranitidine by intragastric adminstration and rats in group D were treated with herbal cake-partitioned moxibustion at Zusanli （ST 36） and Zhongwan （CV 12）, for 8 d. Excepted for rats in group A, all the other rats were treated with indomethacin at 5 mg/（kg.bw） at 8：00 a.m. on the second day after finishing all the intervention and sacrificed 7 h later to isolate the stomach. Histopathological changes of the gastric tissues were observed under light microscope after hematoxylin-eosin （HE） staining. The protein expressions of MEK：1/2 and ERK：1/2 in the gastric tissues were detected by immunohistochemistry. Results： After intervention, the gastric mucosal injury in group B was significantly severer than that in group A, with large breakage and ablating; the damage of gastric mucosa was decreased in group C compared with group B; the gastric mucosal surface remained relatively complete, and the status of breakage and ablating was significantly improved. After intervention, compared with group A, the protein expressions of MEK：1/2 and ERK：12 in gastric tissues of the other groups were significantly higher （P〈0.01）. Compared with group B, the protein expressions of MEK：1/2 and ERK：1/2 in group C and D were significantly higher （all P〈0.01）. Compared with group C, the protein expressions of MEK：1/2 and ERK：1/2 in group D were significantly higher （P〈0.01）. Conclusion： Herbal cake-partitioned moxibustion promotes the repair of gastric mucosa in rats with spleen deficiency syndrome, via improving protein expressions of MEK：1/2 and ERK：1/2 in gastric tissues, as well as activating MEK/ERK signaling pathway.

Influence of different-distance mild moxibustion at Zusanli(ST 36) on functional brain imaging in healthy population

Objective： To explore the beneficial regulatory effect of mild moxibustion from different distances at Zusanli（ST 36） of healthy population on the functions of temperature-related brain regions. Methods： In 20 recruited healthy subjects, the change of the temperature-related brain regions induced by mild moxibustion from different distances at Zusanli（ST 36） was observed by functional magnetic resonance imaging（fM RI）. Results： In comparison of the values in amplitude of low-frequency fluctuation（fA LFF） during and before moxibustion, it has been found that in moxibustion of 2 cm distance, fA LFF value increased in the brain regions of the left anterior cingulated cortex and lateral surrounding cerebral regions, and fA LFF value decreased in the cerebral regions of the peripheral cortex of the calcarine fissure; in moxibustion of 3 cm distance, fA LFF value increased in the brain regions of the right and medial side and paracingulated gyrus, and fA LFF value decreased in the cerebral zone of the left middle temporal gyrus; in moxibustion of 4 cm distance, fA LFF value increased in the brain regions of the right and medial and paracingulated gyrus; and in moxibustion of 5 cm distance, fA LFF value increased in the brain regions of the left hippocampus. In comparison of the value of regional homogeneity（ReH o）, it has been found that in moxibustion of 2 cm distance, ReH o value increased in the cerebral zone of the posterior lobe of the right cerebellum, and ReH o value decreased in the cerebral zone of the right occipital lobe; in moxibustion of 3 cm distance, ReH o value increased in the brain regions of the left cerebellar posterior lobe and left frontal lobe, and ReH o value decreased in the cerebral zone of the right inferior temporal gyrus; in moxibustion of 4 cm distance, ReH o value increased in the brain regions of the right superior frontal gyrus and ReH o value decreased in the brain regions of the right parietal lobe and angular gyrus; in moxibustion of 5 cm distance, ReH o value increased in the cerebral zone of the right frontal lobe and ReH o value decreased in the cerebral zone of the right brainstem. Conclusion： In moxibustion of 3 cm distance, the changes in the brain regions basically conform to the transmission route of body trunk temperature.

Investigating the effects of moxibustion on serum metabolism in healthy human body based on the ~1 H NMR metabolomics technology

Objective： To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance （1H NMR） metabolomics technology, and to find the differences in metabolites, as well as to elucidate the effects of moxibustion on healthy human body from the viewpoint of global metabolism. Methods： Sixty subjects of healthy young men from the enrolled students were randomly divided into a moxibustion group and a control group using random number table, with 30 cases in each group. Subjects in the moxibustion group accepted mild moxibustion on the right Zusanli （ST 36）, once a day, 15 min for each time, and continuous treatment for 10 d; those in the control group did not receive any intervention. There were 28 cases in the moxibustion group and 23 cases in the control group after interventions. On the 2st day, 5th day and lOth day of the intervention, serum samples were collected from subjects of the two groups, and metabolic spectra were obtained by the 1H NMR technology. Results： Before and after the intervention, serum 1H NMR of the moxibustion group was significantly different, while the difference was insignificant in the control group. Metabolite changes in the moxibustion group were mainly in low density lipoprotein （LDL）/very low density lipoprotein （VLDL）, valine, isoleucine, leucine, lactic acid, glutamine, citric acid, polyunsaturated fatty acids, creatine, glycine, glycerol, glucose, tyrosine, histidine, formic acid, alanine, lysine, acetic acid, and glutamic acid. Conclusion： Moxibustion can cause changes of serum metabolic patterns in healthy human by influencing the concentrations of branched-chain amino acids, polyunsaturated fatty acids, and other metabolites to strengthen body＇s metabolisms of amino acids and fatty acid.

Instant effect of moxibustion at different points on electrogastrogram of patients with functional dyspepsia

Objective： To observe different effects of moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） on electrogastrogram （EGG） of patients with functional dyspepsia （FD）. Methods： A total of 65 cases were randomized into a Zusanli （ST 36） group （n=33） and a Yanglingquan （GB 34） group （n=32） by random serial number, to receive moxibustion therapy to the corresponding point respectively. The 8-lead EGG was used before and after treatment to collect EGG parameter of the gastric body, antrum of stomach, lesser curvature and greater curvature, including the dominant frequency, slow wave percentage, average frequency, response area and motility index. Results： After treatment, the dominant frequency and slow wave percentage were improved in nearly the whole stomach area in Zusanli （ST 36） group, showing statistical significances （both P〈0.05）; the slow wave percentage and average frequency were improved in stomach body and antrum of stomach in Yanglingquan （GB 34） group, showing statistical significances （both P〈0.05）; moxibustion at Zusanli （ST 36） had a significantly higher influence on EGG than Yanglingquan （GB 34）, in which the lifting of slow wave percentage was significantly higher than Yanglingquan （GB 34） group （P〈0.05）. Conclusion： Moxibustion at Zusanli （ST 36） and Yanglingquan （GB 34） has the effect of adjusting abnormal EGG pattern in FD patients. Compared with Yanglingquan （GB 34）, Zusanli （ST 36） has a superior effect in improving EGG in stomach area.

Effect of moxibustion stimulation on repair of injured gastric mucosa after common peroneal nerve transection

Objective： To investigate the protective effect of moxibustion in initiating the endogenous protection information on gastric mucosa, and its relationship with the pathway of common peroneal nerve. Methods： Forty-eight Sprague-Dawley（SD） rats were randomly divided into a normal group（group A）, a model group（group B）, a moxibustion model group（group C） and a moxibustion model plus surgery group（group D）, 12 in each group. Except for group A, rats in the other groups were treated with dehydrated ethanol and aspirin to prepare gastric mucosal damage model. The rats in group B were not treated with any interventions; rats in group C received moxibustion at Zusanli（ST 36）, twice a day for continuous 3 d. The rats in group D were subjected to preparing the gastric mucosal damage model after the common peroneal nerve transection, followed by moxibustion at Zusanli（ST 36）. After a 3-day intervention, ulcer index（UI） in each group was observed, and the levels of gastric mucosa-related repair cytokines of tumor necrosis factor-α（TNF-α）, interleukin-4（IL-4） and heat shock protein 70（HSP70） were detected. Results： Compared with group A, the pathological changes and UI of group B were worse（P=0.000）, but TNF-α in serum and tissue was changed significantly（P=0.000, P=0.002）, IL-4 in serum and tissue was improved significantly（P=0.000, P=0.000）. Compared with group B, TNF-α and IL-4 in group C and group D were significantly improved（TNF-α： P=0.003, P=0.016; IL-4： P=0.000, P=0.002）. Compared with group C, the changes of UI in group B and group D were poor（both P=0.000）; the levels of TNF-α and IL-4 in serum were significantly decreased（TNF-α： P=0.000, P=0.025; IL-4： P=0.000, P=0.034）; and tissue HSP70 levels were decreased significantly（P=0.000, P=0.033）. Conclusion： Zusanli（ST 36） can transmit information through the pathway of common peroneal nerve, regulate the release of gastric mucosal protective factors, and up-regulate the expression of cytothesis-related proteins, so as to achieve the effect in repairing gastric mucosa.

Effect of moxibustion at Shenque(CV 8)on myocardial remodeling and function in exercise-induced fatigue rats

Objective To explore the effect of moxibustion at Shenque(CV 8)on myocardial structure and function in exercise-induced fatigue rats.Methods A 12-week treadmill running training was performed to create an exercise-induced fatigue rat model.Sixty eligible male specific-pathogen-free grade Sprague-Dawley rats were randomly divided into a blank group,a control group,a model group,a non-meridian non-acupoint group,a Zusanli(ST 36)group and a Shenque(CV 8)group,with 10 rats in each group.Rats in the blank group did not receive treadmill running training or moxibustion.Rats in the control group did not receive treadmill running training but received mild moxibustion at Shenque(CV 8).Rats in the model group received treadmill running training but no moxibustion.Rats in the non-meridian non-acupoint group,the Zusanli(ST 36)group and the Shenque(CV 8)group received moxibustion at the non-meridian non-acupoint points,Zusanli(ST 36)or Shenque(CV 8)immediately after each treadmill running training,15 min each time,once a day for 5 consecutive days a week at a 2-day interval,60 times of moxibustion in total.Left ventricular end-diastolic diameter(LVEDd),left ventricular end-systolic diameter(LVESd),left ventricular diastolic volume(LVDv),left ventricular systolic volume(LVSv),ejection fraction(EF),stroke volume(SV),early diastolic peak flow velocity of mitral valve(E)and late diastolic peak flow velocity of mitral valve(A)of each group before and after the last treadmill running training were measured.Blood was collected 6 h after the last treadmill running training,and serum C-reactive protein(CRP),myoglobin(Mb),creatine kinase-myocardial band(CK-MB),cardiac troponin I(cTnI)and cardiac troponin T(cTnT)levels were detected.Finally,the heart was separated,the heart mass(HM)was measured,the cTnT level of the myocardial tissue was detected,the ultrastructural changes of the left ventricular myocardium were observed by transmission electron microscope,the left ventricular fraction shortening(LVFS),E/A and heart mass index(HMI)were calculated.Results Compared with the same group before treatment,the rat cardiac LVEDd,LVESd,LVDv,LVSv,SV,E and A were significantly increased(all P<0.01),and the rat LVFS,E/A and EF were significantly decreased(all P<0.01)in the model group and the non-meridian non-acupoint group after treatment;the rat cardiac SV,LVDv,LVSv,E and A were all increased(all P<0.01),while E/A and EF were decreased(all P<0.01)in the Zusanli(ST 36)group after treatment;the rat cardiac LVDv,E and A were significantly increased(P<0.01 or P<0.05),and E/A was significantly decreased(P<0.01)in the Shenque(CV 8)group after treatment.After treatment,compared with the blank group,the rat cardiac LVEDd,LVESd,SV,LVDv,LVSv,E,A,the serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and the myocardial cTnT were increased(all P<0.01),and the LVFS,E/A and EF were all reduced(all P<0.01)in the model group;compared with the model group and the non-meridian non-acupoint group,rats in the Zusanli(ST 36)group and the Shenque(CV 8)group showed decreased LVEDd,LVESd,SV,LVDv,LVSv,E,A,serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and myocardial cTnT(P<0.01 or P<0.05),along with increased LVFS,E/A and EF(all P<0.01);compared with the Zusanli(ST 36)group,Mb and A of the Shenque(CV 8)group were decreased(both P<0.01),while both E/A and EF were increased(P<0.01,P<0.05).Transmission electron microscopy examination showed that myofibrils in the blank group and the control group were neatly arranged with clear light and dark bands;the model group and the non-meridian non-acupoint group showed different degrees of myofibril disintegration and breakage,increased and aggregated mitochondria of different sizes,and increased electron density.The myofibrils in the Shenque(CV 8)group and Zusanli(ST 36)group were arranged neatly with clear light and dark bands,and compensatory hyperplasia of mitochondria.Conclusion Moxibustion at Shenque(CV 8)and Zusanli(ST 36)both can effectively improve the occurrence of myocardial remodeling in exercise-induced fatigue rats,and the effect of moxibustion at Shenque(CV 8)is better in improving cardiac function.

Effects of Acupuncture and Moxibustion on MDA, GSH-Px, and Hyp in the Skin of Senile Mice

Objective： To observe the effects of acupuncture and moxibustion on the skin histomorphology of senile mice. Methods： The subacute senile model of mouse was made by subcutaneous injection of D-galactose into the neck, then they were randomly allocated into an acupuncture group, a moxibustion group, a model group, and a blank group, and measured the content of malondialdehyde （MDA）, activity of glutathione peroxidase （GSH-Px）, and content of hydroxyproline （Hyp） in the skin tissue. Results： Acupuncture and moxibustion at Zusanli （ST 36） could decrease the content of MDA, enhance the activity of GSH-Px, and increase the content of Hyp in the skin tissue of senile mice. Conclusion： Acupuncture and moxibustion at Zusanli （ST 36） could delay the senescence progress of mice＇s skin.