Aim To develop a high speed and high resolution dynamic rangefinding device for the measurement of large distances.Methods The device was comprised of an ultrasonic transmitter and a receiver,and a receiver , and a co...Aim To develop a high speed and high resolution dynamic rangefinding device for the measurement of large distances.Methods The device was comprised of an ultrasonic transmitter and a receiver,and a receiver , and a continuous ultrasonic wave amplitude-modulated by a low- frequency acoustic signal was used. The rangefinding was achieved by detecting the phase difference between the transmitted and received ultrasonic signals. The design principle. hard- ware implementation , experimental results and performance analysis of the device are included. Results and Conclusion Experiments show that the accuracy of the device are included. within 1.5m while its dynamic data update rate can be up to 40kHz.展开更多
Elucidating protein translational regulation is crucial for understanding cellular function and drug development.A key molecule in protein translation is ribosome,which is a super-molecular complex extensively studied...Elucidating protein translational regulation is crucial for understanding cellular function and drug development.A key molecule in protein translation is ribosome,which is a super-molecular complex extensively studied for more than a half century.The structure and dynamics of ribosome complexes were resolved recently thanks to the development of X-ray crystallography,Cryo-EM,and single molecule biophysics.Current studies of the ribosome have shown multiple functional states,each with a unique conformation.In this study,we analyzed the RNA-protein distances of ribosome(2.5 MDa)complexes and compared these changes among different ribosome complexes.We found that the RNA-protein distance is significantly correlated with the ribosomal functional state.Thus,the analysis of RNA-protein binding distances at important functional sites can distinguish ribosomal functional states and help understand ribosome functions.In particular,the mechanism of translational attenuation by nascent peptides and antibiotics was revealed by the conformational changes of local functional sites.展开更多
文摘Aim To develop a high speed and high resolution dynamic rangefinding device for the measurement of large distances.Methods The device was comprised of an ultrasonic transmitter and a receiver,and a receiver , and a continuous ultrasonic wave amplitude-modulated by a low- frequency acoustic signal was used. The rangefinding was achieved by detecting the phase difference between the transmitted and received ultrasonic signals. The design principle. hard- ware implementation , experimental results and performance analysis of the device are included. Results and Conclusion Experiments show that the accuracy of the device are included. within 1.5m while its dynamic data update rate can be up to 40kHz.
基金partially supported by National Institute of Health(R21/R33-GM078601 and R01-GM100701)National Science Foundation(MCB-1151343)in the US
文摘Elucidating protein translational regulation is crucial for understanding cellular function and drug development.A key molecule in protein translation is ribosome,which is a super-molecular complex extensively studied for more than a half century.The structure and dynamics of ribosome complexes were resolved recently thanks to the development of X-ray crystallography,Cryo-EM,and single molecule biophysics.Current studies of the ribosome have shown multiple functional states,each with a unique conformation.In this study,we analyzed the RNA-protein distances of ribosome(2.5 MDa)complexes and compared these changes among different ribosome complexes.We found that the RNA-protein distance is significantly correlated with the ribosomal functional state.Thus,the analysis of RNA-protein binding distances at important functional sites can distinguish ribosomal functional states and help understand ribosome functions.In particular,the mechanism of translational attenuation by nascent peptides and antibiotics was revealed by the conformational changes of local functional sites.
