[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the ...[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...展开更多
Remarkable achievements have been made in China's agricultural development since the late 1970s. Nevertheless, deep-rooted problems are arising from inefficient farming practices. From mid- and long-term perspectives...Remarkable achievements have been made in China's agricultural development since the late 1970s. Nevertheless, deep-rooted problems are arising from inefficient farming practices. From mid- and long-term perspectives, migration of China's agricultural labor will slow and large-scale operation of farmland will accelerate. Although grain supply and demand have maintained a tight balance, new potentials still exist in improving grain production. Under the pressures of resource and environmental constraints, advancing the green and efficient transition of agriculture will become a new trend in China's agricultural development. In this context, the treatment of agricultural pollution, supply-side structural reform and innovation of agricultural subsidy policy are of strategic significance to the transition and upgrade of China's agriculture.展开更多
Applications are limited at present because the currently available ultrasonic motors (USMs) do not provide suffi-ciently high torque and power. The conventional travelling-wave USM needs the bearing to support, which...Applications are limited at present because the currently available ultrasonic motors (USMs) do not provide suffi-ciently high torque and power. The conventional travelling-wave USM needs the bearing to support, which required lubricant. To solve the above problem, a bearingless travelling-wave USM is designed. First, a novel structure of the two-sided USM consisting of a two-sided teeth stator and two disk-type rotors is designed. And the excitation principle of the two-sided travelling-wave USM is analyzed. Then, using ANSYS software, we set up the model of the stator to predict the excitation frequency and modal response of the stator. The shape of the vibration mode was obtained. Last, the load characteristics of the USM are measured using ex-perimental method. The maximum stall torque and the no-load speed were obtained. The results showed that the characteristics of the two-sided USM are better than those of the conventional one-sided USM.展开更多
We give the topology changing of the silhouette in 3D space while others study the projections in an image. Silhou- ettes play a crucial role in visualization, graphics and vision. This work focuses on the global beha...We give the topology changing of the silhouette in 3D space while others study the projections in an image. Silhou- ettes play a crucial role in visualization, graphics and vision. This work focuses on the global behaviors of silhouettes, especially their topological evolutions, such as splitting, merging, appearing and disappearing. The dynamics of silhouettes are governed by the topology, the curvature of the surface, and the view point. In this paper, we work on a more theoretical level to give enu- merative properties of the silhouette including: the integration of signed geodesic curvature along a silhouette is equal to the view cone angle; in elliptic regions, no silhouette can be contained in another one; in hyperbolic regions, if a silhouette is homotopic to a point, then it has at least 4 cusps; finally, critical events can only happen when the view point is on the aspect surfaces (ruled surface of the asymptotic lines of parabolic points with surface itself). We also introduce a method to visualize the evolution of silhouettes, especially all the critical events where the topologies of the silhouettes change. The results have broad applications in computer vision for recognition, graphics for rendering and visualization.展开更多
The genetically modified high-oleic rapeseed (Brassica napus L.) line W-4 was obtained by transforming a binary vector which harbored an inverted repeat expression cassette of fad2 gene into the rapeseed cultivar We...The genetically modified high-oleic rapeseed (Brassica napus L.) line W-4 was obtained by transforming a binary vector which harbored an inverted repeat expression cassette of fad2 gene into the rapeseed cultivar Westar.The transformation was mediated by Agrobacterium.The flanking sequences to both the left and right borders of T-DNA insertion site were amplified by thermal asymmetric interlaced PCR (TAIL-PCR) from the genomic DNA of the transgenic rapeseed line W-4.The flanking sequences to the right border was 290 bp in length and the nucleotide composition was 31.27% for G+C content while 68.73% for A+T content.The flanking sequence to the left border was 365 bp in length and the G+C content was 32.6% and the A+T content was 67.4%,indicating that the T-DNA was integrated in the A/T-rich region.Further more,sequence alignment analysis showed a deletion of 62 bp including the right border of pCNFIRnos and the integration of the whole left border except a change of G to A.That was to say,the integration of the T-DNA in the transgenic line W-4 not involved in the vector sequences.Based on both flanking sequences as well as the left and right borders of the T-DNA sequences,two pairs of specific primers TLF/TLR and TRF/TRR were designed.Using the primers the event-specific PCR detection method for transgenic rapeseed line W-4 was established.By the PCR,two fragments of 485 and 405 bp were amplified from the W-4 genomic DNA as expected,while no products were amplified from the genomic DNA of other transgenic rapeseed lines and non-transgenic rapeseed line.And by the PCR it is possible to detect the W-4 genomic DNA from a mixed sample of genomic DNA.The limit of the detection for the qualitative PCR assay was 0.1%.The method developed in this work is highly specific,sensitive and suitable for event-specific detection of the transgenic rapeseed line W-4.展开更多
The Agrobacterium mediated transgenic rice ( Oryza saliva L.) population with inserts of maize transposon Activator/Dissociation (Ac/Ds) was investigated. DNA sequences flanking the T-DNA were analyzed with inverse PC...The Agrobacterium mediated transgenic rice ( Oryza saliva L.) population with inserts of maize transposon Activator/Dissociation (Ac/Ds) was investigated. DNA sequences flanking the T-DNA were analyzed with inverse PCR. Results showed that 65.4% of the T-DNA was integrated in different locations of rice genome, and some T-DNA flanking sequences were located on certain chromosomes. A number of T-DNA was found to have inserted into protein coding regions. In order to induce transposition of the inserted Ds elements, 354 crosses of Ac x Ds and Ds x Ac were constructed. The excision frequency of Ds element trans-activated by Ac transposase was 22.7% in the F-2 populations, and the transposition was confirmed with analyses of DNA sequences flanking the Ds elements. In addition to the transposition due to 'cut-paste' mechanism, Ds can replicate itself and integrate into a new locus, and inaccurate excisions were also found. A proportion of DNA segments flanking the Ds elements showed no homologies to sequences published in GenBank, of which two were registered under the accession numbers AF355153 and AF355770. The strategy of using transposon tagging for rice genomics study was discussed.展开更多
Compared with the first two energy transitions in human history, the current third energy transition is characterized by the changing concepts of development. Considering its energy mix dominated by fossil fuels, Chin...Compared with the first two energy transitions in human history, the current third energy transition is characterized by the changing concepts of development. Considering its energy mix dominated by fossil fuels, China is faced with a daunting task of transition. This paper discusses the following policy recommendations on China's energy transition, including building a renewables-friendly electric power system, developing smart grids and electric vehicles, promoting cross-regional electric power transactions, encouraging financial innovation, and creating new energy industry investment funds to broaden financing channels and diversify investment entities.展开更多
Based on the analysis of conditions for airflow reversal within a bypass branch, the airflow reversal phenomena were successfully simulated in the laboratory experiments. Data obtained indicate that airflow reversal w...Based on the analysis of conditions for airflow reversal within a bypass branch, the airflow reversal phenomena were successfully simulated in the laboratory experiments. Data obtained indicate that airflow reversal within a bypass branch is mainly caused by fire pressure yielded in the combustion branch. In addition, the maximum reversal quantity of airflow occurs in step with the highest temperature of fire, the highest concentration of CO 2 and the lowest concentration of O 2. Moreover, the time that fire size reaches the maximum value corresponds to the time airflow reversal in the bypass branch is most possible. The results are of great significance for guiding rescue work to avoid airflow reversal.展开更多
LEF1/TCFs are high mobility group box-containing transcriptional factors mediating canonical Wnt/β-catenin signaling during early embryogenesis and tumorigenesis. β-Catenin forms a complex with LEF 1/TCFs and transa...LEF1/TCFs are high mobility group box-containing transcriptional factors mediating canonical Wnt/β-catenin signaling during early embryogenesis and tumorigenesis. β-Catenin forms a complex with LEF 1/TCFs and transactivates LEF1/TCF-mediated transcriptions during dorsalization. Although LEF-mediated transcription is also implicated in ventralization, the underlying molecular mechanism is not well understood. Using the vertebrate Xenopus laevis model system, we found that Xom, which is a ventralizing homeobox protein with dual roles of transcriptional activation and repression, forms a complex with LEF 1/TCF through its homeodomain and transactivates LEF 1/TCF-mediated transcription through its N-terminal transactivation domain (TAD). Our data show that Xom lacking the N-terminal TAD fails to transactivate ventral genes, such as BMP4 and Xom itself, but retains the ability to suppress transcriptional activation of dorsal gene promoters, such as the Goosecoid promoter, indicating that transactivation and repression are separable functions of Xom. It has been postulated that Xom forms a positive re-enforcement loop with BMP4 to promote ventral- ization and to suppress dorsal gene expression. Consistent with an essential role of Xom transactivation of LEF1/TCFs during early embryogenesis, we found that expression of the dominant-negative Xom mutant that lacks the TAD fails to re-enforce the ventral signaling of BMP4 and causes a catastrophic effect during gastrulation. Our data suggest that the functional interaction of Xom and LEF 1/TCF-factors is essential for ventral cell fate determination and that LEF 1/TCF factors may function as a point of convergence to mediate the combined signaling of Wnt/β-catenin and BMP4/Xom pathways during early embryogenesis.展开更多
Restrained distortional buckling is an important buckling mode of steel-concrete composite box beams(SCCBB)under the hogging moment.Rotational and lateral deformation restraints of the bottom plate by the webs are ess...Restrained distortional buckling is an important buckling mode of steel-concrete composite box beams(SCCBB)under the hogging moment.Rotational and lateral deformation restraints of the bottom plate by the webs are essential factors affecting SCCBB distortional buckling.Based on the stationary potential energy principle,the analytical expressions for the rotational restraint stiffness(RRS)of the web upper edge as well as the RRS and the lateral restraint stiffness(LRS)of the bottom plate were derived.Also,the SCCBB critical moment formula under the hogging moment was derived.Using twenty specimens,the theoretical calculation method is compared with the finite-element method.Results indicate that the theoretical calculation method can effectively and accurately reflect the restraint effect of the studs,top steel flange,and other factors on the bottom plate.Both the RRS and the LRS have a nonlinear coupling relationship with the external loads and the RRS of the web’s upper edge.Under the hogging moment,the RRS of the web upper edge has a certain influence on the SCCBB distortional buckling critical moment.With increasing RRS of the web upper edge,the SCCBB critical moment increases at first and then tends to be stable.展开更多
In the actual idioms teaching, most students feel that idioms are difficult to comprehend, memorize and make actual application just because the overall meanings of idioms are unpredictable. Since cognitive linguistic...In the actual idioms teaching, most students feel that idioms are difficult to comprehend, memorize and make actual application just because the overall meanings of idioms are unpredictable. Since cognitive linguistics is based on embodiment, it can change the abstract things into comparatively concrete things, which provides a shortest way to grasp these idioms. Actually, most idioms are based on conceptual metaphor and metonymy composed of conceptual mappings between the source domain and the target one. Therefore, this paper is trying to probe into the role of cognitive principles in idioms teaching, especially animal idioms from the contrastive analysis on Chinese "hu" idioms and English "lion" idioms.展开更多
基金Supported by National 863 Program of China(2006AA10A113)Natural Science foundation of Zhejiang Province(Y306097)~~
文摘[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...
文摘Remarkable achievements have been made in China's agricultural development since the late 1970s. Nevertheless, deep-rooted problems are arising from inefficient farming practices. From mid- and long-term perspectives, migration of China's agricultural labor will slow and large-scale operation of farmland will accelerate. Although grain supply and demand have maintained a tight balance, new potentials still exist in improving grain production. Under the pressures of resource and environmental constraints, advancing the green and efficient transition of agriculture will become a new trend in China's agricultural development. In this context, the treatment of agricultural pollution, supply-side structural reform and innovation of agricultural subsidy policy are of strategic significance to the transition and upgrade of China's agriculture.
基金Project (No. 50175018) supported by the National Natural ScienceFoundation of China
文摘Applications are limited at present because the currently available ultrasonic motors (USMs) do not provide suffi-ciently high torque and power. The conventional travelling-wave USM needs the bearing to support, which required lubricant. To solve the above problem, a bearingless travelling-wave USM is designed. First, a novel structure of the two-sided USM consisting of a two-sided teeth stator and two disk-type rotors is designed. And the excitation principle of the two-sided travelling-wave USM is analyzed. Then, using ANSYS software, we set up the model of the stator to predict the excitation frequency and modal response of the stator. The shape of the vibration mode was obtained. Last, the load characteristics of the USM are measured using ex-perimental method. The maximum stall torque and the no-load speed were obtained. The results showed that the characteristics of the two-sided USM are better than those of the conventional one-sided USM.
基金Project supported by the NSF CAREER Award (Nos. CCF-0448339 and DMS-0528363) of the USAthe National Natural Science Foundation of China (No. 60503067)
文摘We give the topology changing of the silhouette in 3D space while others study the projections in an image. Silhou- ettes play a crucial role in visualization, graphics and vision. This work focuses on the global behaviors of silhouettes, especially their topological evolutions, such as splitting, merging, appearing and disappearing. The dynamics of silhouettes are governed by the topology, the curvature of the surface, and the view point. In this paper, we work on a more theoretical level to give enu- merative properties of the silhouette including: the integration of signed geodesic curvature along a silhouette is equal to the view cone angle; in elliptic regions, no silhouette can be contained in another one; in hyperbolic regions, if a silhouette is homotopic to a point, then it has at least 4 cusps; finally, critical events can only happen when the view point is on the aspect surfaces (ruled surface of the asymptotic lines of parabolic points with surface itself). We also introduce a method to visualize the evolution of silhouettes, especially all the critical events where the topologies of the silhouettes change. The results have broad applications in computer vision for recognition, graphics for rendering and visualization.
基金Supported by Key Agricultural Technology Research and Development Program of Jiangsu Province(BE2009304)Fund for National Rapeseed Research System(CARS13)~~
文摘The genetically modified high-oleic rapeseed (Brassica napus L.) line W-4 was obtained by transforming a binary vector which harbored an inverted repeat expression cassette of fad2 gene into the rapeseed cultivar Westar.The transformation was mediated by Agrobacterium.The flanking sequences to both the left and right borders of T-DNA insertion site were amplified by thermal asymmetric interlaced PCR (TAIL-PCR) from the genomic DNA of the transgenic rapeseed line W-4.The flanking sequences to the right border was 290 bp in length and the nucleotide composition was 31.27% for G+C content while 68.73% for A+T content.The flanking sequence to the left border was 365 bp in length and the G+C content was 32.6% and the A+T content was 67.4%,indicating that the T-DNA was integrated in the A/T-rich region.Further more,sequence alignment analysis showed a deletion of 62 bp including the right border of pCNFIRnos and the integration of the whole left border except a change of G to A.That was to say,the integration of the T-DNA in the transgenic line W-4 not involved in the vector sequences.Based on both flanking sequences as well as the left and right borders of the T-DNA sequences,two pairs of specific primers TLF/TLR and TRF/TRR were designed.Using the primers the event-specific PCR detection method for transgenic rapeseed line W-4 was established.By the PCR,two fragments of 485 and 405 bp were amplified from the W-4 genomic DNA as expected,while no products were amplified from the genomic DNA of other transgenic rapeseed lines and non-transgenic rapeseed line.And by the PCR it is possible to detect the W-4 genomic DNA from a mixed sample of genomic DNA.The limit of the detection for the qualitative PCR assay was 0.1%.The method developed in this work is highly specific,sensitive and suitable for event-specific detection of the transgenic rapeseed line W-4.
文摘The Agrobacterium mediated transgenic rice ( Oryza saliva L.) population with inserts of maize transposon Activator/Dissociation (Ac/Ds) was investigated. DNA sequences flanking the T-DNA were analyzed with inverse PCR. Results showed that 65.4% of the T-DNA was integrated in different locations of rice genome, and some T-DNA flanking sequences were located on certain chromosomes. A number of T-DNA was found to have inserted into protein coding regions. In order to induce transposition of the inserted Ds elements, 354 crosses of Ac x Ds and Ds x Ac were constructed. The excision frequency of Ds element trans-activated by Ac transposase was 22.7% in the F-2 populations, and the transposition was confirmed with analyses of DNA sequences flanking the Ds elements. In addition to the transposition due to 'cut-paste' mechanism, Ds can replicate itself and integrate into a new locus, and inaccurate excisions were also found. A proportion of DNA segments flanking the Ds elements showed no homologies to sequences published in GenBank, of which two were registered under the accession numbers AF355153 and AF355770. The strategy of using transposon tagging for rice genomics study was discussed.
文摘Compared with the first two energy transitions in human history, the current third energy transition is characterized by the changing concepts of development. Considering its energy mix dominated by fossil fuels, China is faced with a daunting task of transition. This paper discusses the following policy recommendations on China's energy transition, including building a renewables-friendly electric power system, developing smart grids and electric vehicles, promoting cross-regional electric power transactions, encouraging financial innovation, and creating new energy industry investment funds to broaden financing channels and diversify investment entities.
基金National Natural Science Foundation of China( 5 9876 0 45 )
文摘Based on the analysis of conditions for airflow reversal within a bypass branch, the airflow reversal phenomena were successfully simulated in the laboratory experiments. Data obtained indicate that airflow reversal within a bypass branch is mainly caused by fire pressure yielded in the combustion branch. In addition, the maximum reversal quantity of airflow occurs in step with the highest temperature of fire, the highest concentration of CO 2 and the lowest concentration of O 2. Moreover, the time that fire size reaches the maximum value corresponds to the time airflow reversal in the bypass branch is most possible. The results are of great significance for guiding rescue work to avoid airflow reversal.
文摘LEF1/TCFs are high mobility group box-containing transcriptional factors mediating canonical Wnt/β-catenin signaling during early embryogenesis and tumorigenesis. β-Catenin forms a complex with LEF 1/TCFs and transactivates LEF1/TCF-mediated transcriptions during dorsalization. Although LEF-mediated transcription is also implicated in ventralization, the underlying molecular mechanism is not well understood. Using the vertebrate Xenopus laevis model system, we found that Xom, which is a ventralizing homeobox protein with dual roles of transcriptional activation and repression, forms a complex with LEF 1/TCF through its homeodomain and transactivates LEF 1/TCF-mediated transcription through its N-terminal transactivation domain (TAD). Our data show that Xom lacking the N-terminal TAD fails to transactivate ventral genes, such as BMP4 and Xom itself, but retains the ability to suppress transcriptional activation of dorsal gene promoters, such as the Goosecoid promoter, indicating that transactivation and repression are separable functions of Xom. It has been postulated that Xom forms a positive re-enforcement loop with BMP4 to promote ventral- ization and to suppress dorsal gene expression. Consistent with an essential role of Xom transactivation of LEF1/TCFs during early embryogenesis, we found that expression of the dominant-negative Xom mutant that lacks the TAD fails to re-enforce the ventral signaling of BMP4 and causes a catastrophic effect during gastrulation. Our data suggest that the functional interaction of Xom and LEF 1/TCF-factors is essential for ventral cell fate determination and that LEF 1/TCF factors may function as a point of convergence to mediate the combined signaling of Wnt/β-catenin and BMP4/Xom pathways during early embryogenesis.
基金Projects(U1934207,52078487,51778630) supported by the National Natural Science Foundations of ChinaProject(502501006) supported by the Fundamental Research Funds for the Central Universities,ChinaProject(2019RS3009) supported by the Hunan Innovative Provincial Construction Project,China。
文摘Restrained distortional buckling is an important buckling mode of steel-concrete composite box beams(SCCBB)under the hogging moment.Rotational and lateral deformation restraints of the bottom plate by the webs are essential factors affecting SCCBB distortional buckling.Based on the stationary potential energy principle,the analytical expressions for the rotational restraint stiffness(RRS)of the web upper edge as well as the RRS and the lateral restraint stiffness(LRS)of the bottom plate were derived.Also,the SCCBB critical moment formula under the hogging moment was derived.Using twenty specimens,the theoretical calculation method is compared with the finite-element method.Results indicate that the theoretical calculation method can effectively and accurately reflect the restraint effect of the studs,top steel flange,and other factors on the bottom plate.Both the RRS and the LRS have a nonlinear coupling relationship with the external loads and the RRS of the web’s upper edge.Under the hogging moment,the RRS of the web upper edge has a certain influence on the SCCBB distortional buckling critical moment.With increasing RRS of the web upper edge,the SCCBB critical moment increases at first and then tends to be stable.
文摘In the actual idioms teaching, most students feel that idioms are difficult to comprehend, memorize and make actual application just because the overall meanings of idioms are unpredictable. Since cognitive linguistics is based on embodiment, it can change the abstract things into comparatively concrete things, which provides a shortest way to grasp these idioms. Actually, most idioms are based on conceptual metaphor and metonymy composed of conceptual mappings between the source domain and the target one. Therefore, this paper is trying to probe into the role of cognitive principles in idioms teaching, especially animal idioms from the contrastive analysis on Chinese "hu" idioms and English "lion" idioms.