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肾母细胞瘤过度表达基因对肾小管转分化机制的影响
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作者 赵燕 尹爱平 《现代医药卫生》 2012年第9期1327-1328,共2页
目的通过肾母细胞瘤过度表达基因(CCN3)对体外培养转化生长因子β1(TGF-β1)参与人肾小管上皮细胞株(HK-2)转分化机制的影响,探讨CCN3的作用及可能途径。方法利用体外细胞培养技术培养HK-2,并将其分为3组,正常对照组,TGF-β1组、10μg/L... 目的通过肾母细胞瘤过度表达基因(CCN3)对体外培养转化生长因子β1(TGF-β1)参与人肾小管上皮细胞株(HK-2)转分化机制的影响,探讨CCN3的作用及可能途径。方法利用体外细胞培养技术培养HK-2,并将其分为3组,正常对照组,TGF-β1组、10μg/L,CCN3组、50μg/L。利用细胞免疫技术,检测3组的上皮细胞标志性蛋白钙黏蛋白E-cadherin的表达,激光共聚焦显微镜下观察并扫描成像。利用IMS图像分析系统对扫描图像进行分析。结果 TGF-β1组E-cadherin的表达明显低于正常对照组(P<0.05),CCN3组的肾小管上皮细胞在加入CCN3及TGF-β1后,E-cadherin的表达明显高于TGF-β1组(P<0.05)。结论 TGF-β1加速肾小管转分化机制,CCN3能延缓TGF-β1所致的肾小管转分化进程。 展开更多
关键词 转化生长因子Β1 WILMS瘤 肾小管 肾母细胞瘤过度表达基因 转分化机制
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The Mechanism of the Acclimation of Nannochloropsis oceanica to Freshwater Deduced from Its Transcriptome Profiles 被引量:3
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作者 GUO Li YANG Guanpin 《Journal of Ocean University of China》 SCIE CAS 2015年第5期922-930,共9页
In this study, we compared the transcriptomes of Nannochloropsis oceanica cultured in f/2 medium prepared with seawater and freshwater, respectively, aiming to understand the acclimation mechanism of this alga to fres... In this study, we compared the transcriptomes of Nannochloropsis oceanica cultured in f/2 medium prepared with seawater and freshwater, respectively, aiming to understand the acclimation mechanism of this alga to freshwater. Differentially expressed genes were mainly assigned to the degradation of cell components, ion transportation, and ribosomal biogenesis. These find- ings indicate that the algal cells degrade its components (mainly amino acids and fatty acids) to yield excessive energy (ATP) to maintain cellular ion (mainly K+ and Ca〉) homeostasis, while the depletion of amino acids and ATP, and the reduction of ribosomes attenuate the protein translation and finally slow down the cell growth. 展开更多
关键词 Nannochloropsis oceanica ACCLIMATION ion depletion ribosome biogenesis protein translation
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