Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of...Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of porous titanium coated with insulin-like growth factor-1（IGF-1） and transforming growth factor-β1（TGF-β1） gelatin microspheres on the function of MG63 cells were evaluated in vitro.The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity.The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg,respectively.The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg,respectively.The joint application of IGF-1 and TGF-β1 group,which promote adhesion,proliferation and differentiation of MG63 cells,is superior to a single application group.展开更多
Objective: In this research, we studied the TGF-β1 effects on connexin-43 expression in cultured human bladder smooth muscle cells. Methods: Human bladder smooth muscle cells primary cultures, with bladder tissue obt...Objective: In this research, we studied the TGF-β1 effects on connexin-43 expression in cultured human bladder smooth muscle cells. Methods: Human bladder smooth muscle cells primary cultures, with bladder tissue obtained from patients undergoing cystectomy, were intervened by recombinant human TGF-β1. Connexin-43 expression in human bladder smooth muscle cells was then examined by Western blotting and immunocytochemistry. Results: Stimulation with TGF-β1 led to significant reduction of connexin-43 immunoreactivity and coupling (P<0.0001). Connexin-43 protein expression was significantly downregulated (P<0.05). Simultaneously, low phosphorylation species of connexin-43 were particularly affected. Conclusion: Our experiments demonstrated a significant downregulation of connexin-43 by TGF-β1 in cultured human bladder smooth muscle cells. These findings support the view that TGF-β1 is involved in the pathophysiology of urinary bladder dysfunction.展开更多
目的:本研究从细胞因子角度出发探讨白介素-13(Interleukin-13,IL-13)、转化生子因子-β1(Transforming growthfactor-β1,TGF-β1)、血管内皮生子因子(Vascular endothelial growth factor,VEGF)血清浓度是否在哮喘患者的气道慢性炎症...目的:本研究从细胞因子角度出发探讨白介素-13(Interleukin-13,IL-13)、转化生子因子-β1(Transforming growthfactor-β1,TGF-β1)、血管内皮生子因子(Vascular endothelial growth factor,VEGF)血清浓度是否在哮喘患者的气道慢性炎症及重塑的发病机制中起着调控作用及其可能途径。方法:选择哮喘急性发作期患者36例(中重度发作20例、轻度发作16例)、缓解期36例、健康体检者36例。取肘静脉血,用ELISA方法测定血清中IL-13、TGF-β1、VEGF水平;所有入试者均作肺功能检查,并对结果进行统计学分析。结果:血清IL-13、TGF-β1、VEGF的表达量在哮喘患者中存在显著的正相关性;同时三者血清浓度越高,肺功能检查指标第1 s用力呼气容积(Forced expiratory volume in the 1st s,FEV1)、第1 s用力呼气容积占用力肺活量的比值(Ratio of forced expiratory volume in 1 s to forced vital capacity,FEV1/FVC%)越小,提示哮喘的病情程度越重,血清IL-13、TGF-β1、VEGF的表达量与FEV1、FEV1/FVC%呈负相关性。结论:IL-13、TGF-β1、VEGF网络失衡是哮喘发病的主要机制之一,三者在哮喘发病中相互影响,相互促进,共同参与哮喘的气道炎症与重塑的过程;联合检测血清IL-13、TGF-β1、VEGF水平对判断哮喘患者病情严重程度及预后、辅助诊断及治疗具有重要价值。展开更多
Objective To examine the effect of neuropeptide Y （NPY） on TGF-β1 production in RAW264.7 macrophages. Methods Enzyme linked immunosorbent assay （ELISA） was used to detect TGF-β1 production. Cell counting kit 8 ...Objective To examine the effect of neuropeptide Y （NPY） on TGF-β1 production in RAW264.7 macrophages. Methods Enzyme linked immunosorbent assay （ELISA） was used to detect TGF-β1 production. Cell counting kit 8 （CCK-8） was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85. Results NPY treatment could promote TGF-β1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-β 1 production induced by NPY could be abolished by wortrnannin pretreatment. Conclusion NPY may elicit TGF-β production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.展开更多
目的探讨血栓通注射液联合左卡尼汀对糖尿病肾病患者HGF、Cys-C、TGF-β1及内皮功能影响作用。方法选取2018年1月—2019年12月医院接受治疗的糖尿病肾病患者164例进行回顾性分析,根据不同治疗方式分为对照组和观察组,每组各82例;对照组...目的探讨血栓通注射液联合左卡尼汀对糖尿病肾病患者HGF、Cys-C、TGF-β1及内皮功能影响作用。方法选取2018年1月—2019年12月医院接受治疗的糖尿病肾病患者164例进行回顾性分析,根据不同治疗方式分为对照组和观察组,每组各82例;对照组给予左卡尼汀治疗,观察组在对照组基础上联合血栓通注射液进行治疗;对两组患者治疗后临床效果、治疗前后血糖、血脂水平、肾功能指标、内皮功能以及血清中HGF、Cys-C、TGF-β1水平表达进行比较与分析。结果两组治疗后,观察组有效率为90.24%(74/82),对照组有效率为73.17%(60/82),组间比较,观察组有效率显著高于对照组(P<0.05);两组患者治疗前FBG、2 h PBG、TG、TC比较差异无统计学意义(P>0.05),治疗后两组患者各指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05);两组患者治疗前肾功能指标Scr、BUN、UAER比较无统计学差异(P>0.05),治疗后两组患者各指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05);两组患者治疗前内皮能指标sICAM-1、ET-1比较差异无统计学意义(P>0.05),治疗后两组患者个指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05);两组患者治疗前血清中HGF、Cys-C、TGF-β1比较差异无统计学意义(P>0.05),治疗后两组患者个指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05)。结论血栓通注射液联合左卡尼汀对在糖尿病肾病患者的治疗中可显著改善患者肾功能相关指标和血管内皮功能,有效控制血糖,疗效确切,值得临床推广。展开更多
基金Project(2013zzts306)supported by the Fundamental Research Funds for the Central Universities of Central South University,ChinaProject(225)supported by the High Level Health Personnel in Hunan Province,China
文摘Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of porous titanium coated with insulin-like growth factor-1（IGF-1） and transforming growth factor-β1（TGF-β1） gelatin microspheres on the function of MG63 cells were evaluated in vitro.The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity.The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg,respectively.The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg,respectively.The joint application of IGF-1 and TGF-β1 group,which promote adhesion,proliferation and differentiation of MG63 cells,is superior to a single application group.
文摘Objective: In this research, we studied the TGF-β1 effects on connexin-43 expression in cultured human bladder smooth muscle cells. Methods: Human bladder smooth muscle cells primary cultures, with bladder tissue obtained from patients undergoing cystectomy, were intervened by recombinant human TGF-β1. Connexin-43 expression in human bladder smooth muscle cells was then examined by Western blotting and immunocytochemistry. Results: Stimulation with TGF-β1 led to significant reduction of connexin-43 immunoreactivity and coupling (P<0.0001). Connexin-43 protein expression was significantly downregulated (P<0.05). Simultaneously, low phosphorylation species of connexin-43 were particularly affected. Conclusion: Our experiments demonstrated a significant downregulation of connexin-43 by TGF-β1 in cultured human bladder smooth muscle cells. These findings support the view that TGF-β1 is involved in the pathophysiology of urinary bladder dysfunction.
文摘目的:本研究从细胞因子角度出发探讨白介素-13(Interleukin-13,IL-13)、转化生子因子-β1(Transforming growthfactor-β1,TGF-β1)、血管内皮生子因子(Vascular endothelial growth factor,VEGF)血清浓度是否在哮喘患者的气道慢性炎症及重塑的发病机制中起着调控作用及其可能途径。方法:选择哮喘急性发作期患者36例(中重度发作20例、轻度发作16例)、缓解期36例、健康体检者36例。取肘静脉血,用ELISA方法测定血清中IL-13、TGF-β1、VEGF水平;所有入试者均作肺功能检查,并对结果进行统计学分析。结果:血清IL-13、TGF-β1、VEGF的表达量在哮喘患者中存在显著的正相关性;同时三者血清浓度越高,肺功能检查指标第1 s用力呼气容积(Forced expiratory volume in the 1st s,FEV1)、第1 s用力呼气容积占用力肺活量的比值(Ratio of forced expiratory volume in 1 s to forced vital capacity,FEV1/FVC%)越小,提示哮喘的病情程度越重,血清IL-13、TGF-β1、VEGF的表达量与FEV1、FEV1/FVC%呈负相关性。结论:IL-13、TGF-β1、VEGF网络失衡是哮喘发病的主要机制之一,三者在哮喘发病中相互影响,相互促进,共同参与哮喘的气道炎症与重塑的过程;联合检测血清IL-13、TGF-β1、VEGF水平对判断哮喘患者病情严重程度及预后、辅助诊断及治疗具有重要价值。
文摘Objective To examine the effect of neuropeptide Y （NPY） on TGF-β1 production in RAW264.7 macrophages. Methods Enzyme linked immunosorbent assay （ELISA） was used to detect TGF-β1 production. Cell counting kit 8 （CCK-8） was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85. Results NPY treatment could promote TGF-β1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-β 1 production induced by NPY could be abolished by wortrnannin pretreatment. Conclusion NPY may elicit TGF-β production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.
文摘目的探讨血栓通注射液联合左卡尼汀对糖尿病肾病患者HGF、Cys-C、TGF-β1及内皮功能影响作用。方法选取2018年1月—2019年12月医院接受治疗的糖尿病肾病患者164例进行回顾性分析,根据不同治疗方式分为对照组和观察组,每组各82例;对照组给予左卡尼汀治疗,观察组在对照组基础上联合血栓通注射液进行治疗;对两组患者治疗后临床效果、治疗前后血糖、血脂水平、肾功能指标、内皮功能以及血清中HGF、Cys-C、TGF-β1水平表达进行比较与分析。结果两组治疗后,观察组有效率为90.24%(74/82),对照组有效率为73.17%(60/82),组间比较,观察组有效率显著高于对照组(P<0.05);两组患者治疗前FBG、2 h PBG、TG、TC比较差异无统计学意义(P>0.05),治疗后两组患者各指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05);两组患者治疗前肾功能指标Scr、BUN、UAER比较无统计学差异(P>0.05),治疗后两组患者各指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05);两组患者治疗前内皮能指标sICAM-1、ET-1比较差异无统计学意义(P>0.05),治疗后两组患者个指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05);两组患者治疗前血清中HGF、Cys-C、TGF-β1比较差异无统计学意义(P>0.05),治疗后两组患者个指标较治疗前比较均得到改善,改善情况差异有统计学意义(P<0.05),组间比较,观察组改善效果更显著(P<0.05)。结论血栓通注射液联合左卡尼汀对在糖尿病肾病患者的治疗中可显著改善患者肾功能相关指标和血管内皮功能,有效控制血糖,疗效确切,值得临床推广。
