目的:本研究从细胞因子角度出发探讨白介素-13(Interleukin-13,IL-13)、转化生子因子-β1(Transforming growthfactor-β1,TGF-β1)、血管内皮生子因子(Vascular endothelial growth factor,VEGF)血清浓度是否在哮喘患者的气道慢性炎症...目的:本研究从细胞因子角度出发探讨白介素-13(Interleukin-13,IL-13)、转化生子因子-β1(Transforming growthfactor-β1,TGF-β1)、血管内皮生子因子(Vascular endothelial growth factor,VEGF)血清浓度是否在哮喘患者的气道慢性炎症及重塑的发病机制中起着调控作用及其可能途径。方法:选择哮喘急性发作期患者36例(中重度发作20例、轻度发作16例)、缓解期36例、健康体检者36例。取肘静脉血,用ELISA方法测定血清中IL-13、TGF-β1、VEGF水平;所有入试者均作肺功能检查,并对结果进行统计学分析。结果:血清IL-13、TGF-β1、VEGF的表达量在哮喘患者中存在显著的正相关性;同时三者血清浓度越高,肺功能检查指标第1 s用力呼气容积(Forced expiratory volume in the 1st s,FEV1)、第1 s用力呼气容积占用力肺活量的比值(Ratio of forced expiratory volume in 1 s to forced vital capacity,FEV1/FVC%)越小,提示哮喘的病情程度越重,血清IL-13、TGF-β1、VEGF的表达量与FEV1、FEV1/FVC%呈负相关性。结论:IL-13、TGF-β1、VEGF网络失衡是哮喘发病的主要机制之一,三者在哮喘发病中相互影响,相互促进,共同参与哮喘的气道炎症与重塑的过程;联合检测血清IL-13、TGF-β1、VEGF水平对判断哮喘患者病情严重程度及预后、辅助诊断及治疗具有重要价值。展开更多
Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explan...Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explants from 10-day-old rats were cultured with TGFβ1 or TGFβ2 in the presence of FGF for 5 days, then were examined by light and electron microscopy, and by immunolocal- ization of smooth muscle(α-sm) actin and type I collagen. Results. In TGFβ/FGF-treated explants,extensive proliferation occured, with formation of spindle and star-shaped cells. These cells showed ultrastructure and biochemical features of fibroblast or myofibroblast. Prominent Golgi apparatus and rough endoplaic reticulum were observed in some cells. Intracellular micro- filaments with cytoplasmic dense babies and membrane associated dense bodies, features of smooth muscle cells, were also observed. Some cells showed reactivity to -sin actin antibody. TGFβ/FGF-treated ex- plants were strongly stained with type I collagen antibody. Condusion. In the presence of FGF, TGFβ1 and TGFβ2 induced lens epithelial cell (LEC ) proliferation and transformation into fibroblast or myofibroblast-like cells, with producing of abundant collagen matrix in the explants. The changes are similar to the metaplasia that occurrs in subcapsular opacification of the lens. The findings suggest that TGFβ and FGF plays a role in the pathogenesis of subcapsular opacification of the lens.展开更多
The tumor-promoting arm of transforming growth factor beta(TGF-β)receptor signaling contributes to advanced cancer progression and is considered a master regulator of breast cancer metastasis.In mammals,there are six...The tumor-promoting arm of transforming growth factor beta(TGF-β)receptor signaling contributes to advanced cancer progression and is considered a master regulator of breast cancer metastasis.In mammals,there are six distinct members in the tumor-necrosis factor receptor(TNFR)-associated factor(TRAF)family(TRAF1–TRAF6),with the function of TRAF4 not being extensively studied in the past decade.Although numerous studies have suggested that there is elevated TRAF4 expression in human cancer,it is still unknown in which oncogenic pathway TRAF4 is mainly implicated.This review highlights TGF-β-induced SMAD-dependent signaling and non-SMAD signaling as the major pathways regulated by TRAF4 involved in breast cancer metastasis.展开更多
目的探讨人参多糖注射液联合表柔比星治疗乳腺癌的临床疗效。方法选取2016年2月—2017年2月在临沂市肿瘤医院治疗的乳腺癌患者78例,根据治疗用药的差别分为对照组(39例)和治疗组(39例)。对照组静脉滴注注射用盐酸表柔比星,每个疗程第1...目的探讨人参多糖注射液联合表柔比星治疗乳腺癌的临床疗效。方法选取2016年2月—2017年2月在临沂市肿瘤医院治疗的乳腺癌患者78例,根据治疗用药的差别分为对照组(39例)和治疗组(39例)。对照组静脉滴注注射用盐酸表柔比星,每个疗程第1天给予90 mg/m2,1次/3周;治疗组在对照组基础上静脉滴注人参多糖注射液,4 m L/次,2次/d。两组均治疗6周。观察两组患者临床疗效,同时比较治疗前后两组患者肿瘤标志物水平、血清细胞因子水平和不良反应情况。结果治疗后,对照组客观缓解率(ORR)和临床获益率(CBR)分别为35.90%和69.23%,均分别明显低于治疗组的58.97%和87.18%,两组比较差异具有统计学意义(P<0.05)。治疗后,两组血清糖抗原125(CA125)、糖抗原153(CA153)、癌胚抗原(CEA)、肿瘤坏死因子-α(TNF-α)、可溶性CD105(S-CD105)和转化生子因子-β1(TGF-β1)水平均显著降低(P<0.05),干扰素-γ(IFN-γ)和白细胞介素-12(IL-12)水平显著升高(P<0.05),且治疗组上述肿瘤标志物和细胞因子水平明显优于对照组(P<0.05)。治疗期间,治疗组不良反应发生率明显低于对照组,两组比较差异具有统计学意义(P<0.05)。结论人参多糖注射液联合表柔比星治疗乳腺癌可有效降低机体肿瘤标志物水平和改善机体细胞因子表达水平,具有一定的临床推广应用价值。展开更多
文摘目的:本研究从细胞因子角度出发探讨白介素-13(Interleukin-13,IL-13)、转化生子因子-β1(Transforming growthfactor-β1,TGF-β1)、血管内皮生子因子(Vascular endothelial growth factor,VEGF)血清浓度是否在哮喘患者的气道慢性炎症及重塑的发病机制中起着调控作用及其可能途径。方法:选择哮喘急性发作期患者36例(中重度发作20例、轻度发作16例)、缓解期36例、健康体检者36例。取肘静脉血,用ELISA方法测定血清中IL-13、TGF-β1、VEGF水平;所有入试者均作肺功能检查,并对结果进行统计学分析。结果:血清IL-13、TGF-β1、VEGF的表达量在哮喘患者中存在显著的正相关性;同时三者血清浓度越高,肺功能检查指标第1 s用力呼气容积(Forced expiratory volume in the 1st s,FEV1)、第1 s用力呼气容积占用力肺活量的比值(Ratio of forced expiratory volume in 1 s to forced vital capacity,FEV1/FVC%)越小,提示哮喘的病情程度越重,血清IL-13、TGF-β1、VEGF的表达量与FEV1、FEV1/FVC%呈负相关性。结论:IL-13、TGF-β1、VEGF网络失衡是哮喘发病的主要机制之一,三者在哮喘发病中相互影响,相互促进,共同参与哮喘的气道炎症与重塑的过程;联合检测血清IL-13、TGF-β1、VEGF水平对判断哮喘患者病情严重程度及预后、辅助诊断及治疗具有重要价值。
文摘Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explants from 10-day-old rats were cultured with TGFβ1 or TGFβ2 in the presence of FGF for 5 days, then were examined by light and electron microscopy, and by immunolocal- ization of smooth muscle(α-sm) actin and type I collagen. Results. In TGFβ/FGF-treated explants,extensive proliferation occured, with formation of spindle and star-shaped cells. These cells showed ultrastructure and biochemical features of fibroblast or myofibroblast. Prominent Golgi apparatus and rough endoplaic reticulum were observed in some cells. Intracellular micro- filaments with cytoplasmic dense babies and membrane associated dense bodies, features of smooth muscle cells, were also observed. Some cells showed reactivity to -sin actin antibody. TGFβ/FGF-treated ex- plants were strongly stained with type I collagen antibody. Condusion. In the presence of FGF, TGFβ1 and TGFβ2 induced lens epithelial cell (LEC ) proliferation and transformation into fibroblast or myofibroblast-like cells, with producing of abundant collagen matrix in the explants. The changes are similar to the metaplasia that occurrs in subcapsular opacification of the lens. The findings suggest that TGFβ and FGF plays a role in the pathogenesis of subcapsular opacification of the lens.
基金supported by the Zhejiang University Special Fund for Fundamental Researchthe Fundamental Research Funds for the Central Universities(R14C070002)the Netherlands Organization of Scientific Research grant(MW-NWO 918.66.606),from the Cancer Genomics Centre Netherlands and the Centre for Biomedical Genetics
文摘The tumor-promoting arm of transforming growth factor beta(TGF-β)receptor signaling contributes to advanced cancer progression and is considered a master regulator of breast cancer metastasis.In mammals,there are six distinct members in the tumor-necrosis factor receptor(TNFR)-associated factor(TRAF)family(TRAF1–TRAF6),with the function of TRAF4 not being extensively studied in the past decade.Although numerous studies have suggested that there is elevated TRAF4 expression in human cancer,it is still unknown in which oncogenic pathway TRAF4 is mainly implicated.This review highlights TGF-β-induced SMAD-dependent signaling and non-SMAD signaling as the major pathways regulated by TRAF4 involved in breast cancer metastasis.
文摘目的探讨人参多糖注射液联合表柔比星治疗乳腺癌的临床疗效。方法选取2016年2月—2017年2月在临沂市肿瘤医院治疗的乳腺癌患者78例,根据治疗用药的差别分为对照组(39例)和治疗组(39例)。对照组静脉滴注注射用盐酸表柔比星,每个疗程第1天给予90 mg/m2,1次/3周;治疗组在对照组基础上静脉滴注人参多糖注射液,4 m L/次,2次/d。两组均治疗6周。观察两组患者临床疗效,同时比较治疗前后两组患者肿瘤标志物水平、血清细胞因子水平和不良反应情况。结果治疗后,对照组客观缓解率(ORR)和临床获益率(CBR)分别为35.90%和69.23%,均分别明显低于治疗组的58.97%和87.18%,两组比较差异具有统计学意义(P<0.05)。治疗后,两组血清糖抗原125(CA125)、糖抗原153(CA153)、癌胚抗原(CEA)、肿瘤坏死因子-α(TNF-α)、可溶性CD105(S-CD105)和转化生子因子-β1(TGF-β1)水平均显著降低(P<0.05),干扰素-γ(IFN-γ)和白细胞介素-12(IL-12)水平显著升高(P<0.05),且治疗组上述肿瘤标志物和细胞因子水平明显优于对照组(P<0.05)。治疗期间,治疗组不良反应发生率明显低于对照组,两组比较差异具有统计学意义(P<0.05)。结论人参多糖注射液联合表柔比星治疗乳腺癌可有效降低机体肿瘤标志物水平和改善机体细胞因子表达水平,具有一定的临床推广应用价值。
