In this work,a rotary pump based micromixer for on-chip rapid mixing and liquid transportation is demonstrated and characterized.Both pumping and mixing are realized using a microfluidic chip with a single structural ...In this work,a rotary pump based micromixer for on-chip rapid mixing and liquid transportation is demonstrated and characterized.Both pumping and mixing are realized using a microfluidic chip with a single structural polydimethylsiloxane layer and a portable electric control system.The rotary pump consists of an annular channel and is driven by a motor and magnets.The flow field caused by the peristaltic movement of the channel membrane of the rotary pump is simulated and analyzed.By statistically calculating and comparing the normalized standard deviations of the flow velocity components in a microchannel,it is revealed that up-and-down mixing is the fastest,followed by segment mixing and parallel mixing.Two mixing styles,segment mixing and parallel mixing,were experimentally demonstrated using the chip.The pump achieved 90% of the mixing index in 1 s for the segment mixing type.As for the parallel mixing type,the mixing index was up to 90% after 5 s,which is more than 100-fold improvement compared to conventional mixing by interfacial diffusion.The mixing speeds in both directions were improved prominently by increasing the rotational speed of the pump.展开更多
OBJECTIVE:To investigate the molecular effect of Socheongryong Tang(SCRT,Xiaoqinglong Tang in Chinese) on whole genome level in asthma mouse model by microarray technology.METHODS:Asthma was induced by intranasal inst...OBJECTIVE:To investigate the molecular effect of Socheongryong Tang(SCRT,Xiaoqinglong Tang in Chinese) on whole genome level in asthma mouse model by microarray technology.METHODS:Asthma was induced by intranasal instillation of ovalbumin in mouse.After administration of SCRT on asthma-induced mouse,the expression of genes in lung tissue was measured using whole genome microarray.The functional implication of differentially expressed genes was performed using ontological analysis and the similarity of promoter structure of genes was also analyzed.RESULTS:Treatment of SCRT restored expression level of many up- or down-regulated genes in asthma model,and this recovery rate means SCRT could regulate a set of genes having specific TFBS binding sites.CONCLUSION:In this study,we identified a set of genes subjected to similar regulation by SCRT in asthma model in mice.展开更多
基金supported by the Major State Basic Research Development Program of China ((Grant No. 2007CB310504)the National Natural Science Foundation of China (Grant No. 50730009)
文摘In this work,a rotary pump based micromixer for on-chip rapid mixing and liquid transportation is demonstrated and characterized.Both pumping and mixing are realized using a microfluidic chip with a single structural polydimethylsiloxane layer and a portable electric control system.The rotary pump consists of an annular channel and is driven by a motor and magnets.The flow field caused by the peristaltic movement of the channel membrane of the rotary pump is simulated and analyzed.By statistically calculating and comparing the normalized standard deviations of the flow velocity components in a microchannel,it is revealed that up-and-down mixing is the fastest,followed by segment mixing and parallel mixing.Two mixing styles,segment mixing and parallel mixing,were experimentally demonstrated using the chip.The pump achieved 90% of the mixing index in 1 s for the segment mixing type.As for the parallel mixing type,the mixing index was up to 90% after 5 s,which is more than 100-fold improvement compared to conventional mixing by interfacial diffusion.The mixing speeds in both directions were improved prominently by increasing the rotational speed of the pump.
文摘OBJECTIVE:To investigate the molecular effect of Socheongryong Tang(SCRT,Xiaoqinglong Tang in Chinese) on whole genome level in asthma mouse model by microarray technology.METHODS:Asthma was induced by intranasal instillation of ovalbumin in mouse.After administration of SCRT on asthma-induced mouse,the expression of genes in lung tissue was measured using whole genome microarray.The functional implication of differentially expressed genes was performed using ontological analysis and the similarity of promoter structure of genes was also analyzed.RESULTS:Treatment of SCRT restored expression level of many up- or down-regulated genes in asthma model,and this recovery rate means SCRT could regulate a set of genes having specific TFBS binding sites.CONCLUSION:In this study,we identified a set of genes subjected to similar regulation by SCRT in asthma model in mice.
