Sox genes are transcription factors that ubiquitously exist in animal species, and share a conserved high mobility group(HMG) box. They play important biological roles in diverse developmental processes, such as sex d...Sox genes are transcription factors that ubiquitously exist in animal species, and share a conserved high mobility group(HMG) box. They play important biological roles in diverse developmental processes, such as sex determination and differentiation, chondrogenesis, neurogenesis, and early embryonic development. In this study, we identified 25 sox genes from genome and transcriptome of Japanese flounder Paralichthys olivaceus. These s ox genes could be mainly classified into seven subfamilies(B1, B2, C, D, E, F, and K), and each subfamily exhibited a relatively conserved gene structure. Besides, subfamilies A and G were found exclusively in human and mouse, and sox 32 in subfamily K only existed in teleosts. Compared with other mammals, some s ox genes in teleosts had two duplicates. The loss, duplication, and divergence of sox genes during evolution provided an evidence for whole-genome duplication that occurred in the radiation of teleosts. The expression of Japanese flounder sox genes was also analyzed by FPKM value. Our results showed that certain s ox genes exhibited obviously tissue-specific and spatio-temproal expression. Especially, gonal-basied expression analysis uncovered that s ox7 and s ox2 were ovary-biased, and s ox8 b was testis-biased. Moreover, sox10 a was expressed specifically in ovary, and sox8 a in testis. Therefore this study provide a solid foundation for future functional and evolutionary analysis of sox genes in Japanese flounder.展开更多
Genetic engineering has created many genetically modified （GM） crop varieties that express the cry toxin from the bacterium Bacillus thuringiensis （Bt）. The cry toxin, synthesized during plant growth, has insectic...Genetic engineering has created many genetically modified （GM） crop varieties that express the cry toxin from the bacterium Bacillus thuringiensis （Bt）. The cry toxin, synthesized during plant growth, has insecticidal properties, and can be expressed anywhere in the plant. This study aimed to ascertain the richness and species diversity of edaphic Formicidae and Coleoptera in GM cotton fields compared with the conventional non-transformed cotton crop. We analyzed data from commercial cotton fields located in the municipality of Maracaju, Mato Grosso do Sul, Brazil. The experiment was conducted during the reproductive period of cotton, employed two treatments： Bt cotton and non-Bt cotton. Samples were collected with pitfall traps. Formicidae species richness in the Bt area was lower than in the non-Bt area, but species composition did not differ between the two treatments. Species composition of Coleoptera communities also differed between the treatments because some species were more abundant in the Bt cotton area. On the other hand, the species richness of this group was similar in both areas.展开更多
The achievements of genetical y modified （GM） maize in herbicide toler-ance, insect resistance, disease resistance and alkali resistance were reviewed in this paper so as to provide some reference for the researches...The achievements of genetical y modified （GM） maize in herbicide toler-ance, insect resistance, disease resistance and alkali resistance were reviewed in this paper so as to provide some reference for the researches on molecular breed-ing of maize in China.展开更多
基金Supported by the National Natural Science Foundation of China(No.31672646)the Fundamental Research Funds for the Central Universities(No.201762016)
文摘Sox genes are transcription factors that ubiquitously exist in animal species, and share a conserved high mobility group(HMG) box. They play important biological roles in diverse developmental processes, such as sex determination and differentiation, chondrogenesis, neurogenesis, and early embryonic development. In this study, we identified 25 sox genes from genome and transcriptome of Japanese flounder Paralichthys olivaceus. These s ox genes could be mainly classified into seven subfamilies(B1, B2, C, D, E, F, and K), and each subfamily exhibited a relatively conserved gene structure. Besides, subfamilies A and G were found exclusively in human and mouse, and sox 32 in subfamily K only existed in teleosts. Compared with other mammals, some s ox genes in teleosts had two duplicates. The loss, duplication, and divergence of sox genes during evolution provided an evidence for whole-genome duplication that occurred in the radiation of teleosts. The expression of Japanese flounder sox genes was also analyzed by FPKM value. Our results showed that certain s ox genes exhibited obviously tissue-specific and spatio-temproal expression. Especially, gonal-basied expression analysis uncovered that s ox7 and s ox2 were ovary-biased, and s ox8 b was testis-biased. Moreover, sox10 a was expressed specifically in ovary, and sox8 a in testis. Therefore this study provide a solid foundation for future functional and evolutionary analysis of sox genes in Japanese flounder.
文摘Genetic engineering has created many genetically modified （GM） crop varieties that express the cry toxin from the bacterium Bacillus thuringiensis （Bt）. The cry toxin, synthesized during plant growth, has insecticidal properties, and can be expressed anywhere in the plant. This study aimed to ascertain the richness and species diversity of edaphic Formicidae and Coleoptera in GM cotton fields compared with the conventional non-transformed cotton crop. We analyzed data from commercial cotton fields located in the municipality of Maracaju, Mato Grosso do Sul, Brazil. The experiment was conducted during the reproductive period of cotton, employed two treatments： Bt cotton and non-Bt cotton. Samples were collected with pitfall traps. Formicidae species richness in the Bt area was lower than in the non-Bt area, but species composition did not differ between the two treatments. Species composition of Coleoptera communities also differed between the treatments because some species were more abundant in the Bt cotton area. On the other hand, the species richness of this group was similar in both areas.
基金Supported by Major Science and Technology Special Project of Henan Province(121100110300)~~
文摘The achievements of genetical y modified （GM） maize in herbicide toler-ance, insect resistance, disease resistance and alkali resistance were reviewed in this paper so as to provide some reference for the researches on molecular breed-ing of maize in China.
