A twin ovary mutant derived from the doubled haploid (DH) progeny of a cross,02428/Gui 630, was presumably related to the transposition of some transposable elements. Up to date, all reported the active transposable ...A twin ovary mutant derived from the doubled haploid (DH) progeny of a cross,02428/Gui 630, was presumably related to the transposition of some transposable elements. Up to date, all reported the active transposable elements in rice (Oryza sativa L.) are copia like retrotransposons. In the present study, the reverse transcriptase domains of copia like retrotransposons were amplified from the total DNA isolated from the mutant plants with the degenerated oligonucleotide primers for the domain. Three cloned insert DNAs, R33 1, R33 4 and R33 8, representing putative different copia like retrotransposons were screened out. Two of them displayed high polymorphism between “Zhaiyeqing 8” and “Jingxi 17”. Nine of the polymorphic bands were mapped on seven rice chromosomes. Sequencing analysis revealed that stop codons frequently occur in the sequence of R33 8, while both R33 1 and R33 4 contain a continuous coding region for 81 putative amino acid residues. No significant variation in hybridization patterns was found between indica and japonica rice or among 26 varieties of indica rice when R33 1 was used as a probe. Nevertheless, when R33 4 was used as a probe, high polymorphisms were detected both between indica and japonica rice and among 26 indica varieties, implying that this element might still be active in rice genomes.展开更多
Characterization of energy-transduction on die chloroplast thylakoid membranes from spinach (Spinacia oleracca L.) after thermal pretreatment was investigated. The related reactions of energy-transduction in chloropla...Characterization of energy-transduction on die chloroplast thylakoid membranes from spinach (Spinacia oleracca L.) after thermal pretreatment was investigated. The related reactions of energy-transduction in chloroplasts were seriously affected by thermal pretreatment. The results were obtained as following: (1) The rate of cyclic photophosphorylation declined when the pretreatment temperature increased in the range of 25 to 45 degreesC. (2) The thermal pretreatment led to a decrease of the activity of thylakoid membrane-bounded ATPase. (3) Proton uptake of chloroplasts acid the fluorescence quenching of 9-aminoacridine (9-AA) in thylakoid membrane decreased after the thermal pretreatment, but addition of dicyclohexylcarbodiimide (DCCD) could partially restore the fluorescence quenching of 9-AA. (4) Both the rates of fast phase in electrochroism absorption change at 515 nm and the millisecond delayed light emission (ms-DLE) of chloroplast showed a progressive decrease upon raising the temperature of pretreatment. (5) Immunbloting analysis showed that the thermal pretreatment caused the changes of protein content and the electrophoresis mobility of thylakoid membrane-bound ATPase and its alpha -subunit. (6) If the temperature of pretreatment were higher than 33 degreesC, oxygen uptake of PS I -mediated in the samples was rapidly inhibited, but addition of sinapine into the reaction medium could partially restore the ability of oxygen uptake in the samples. These results are briefly discussed in relation to the change of permeability of thylakoid membranes, the dissociation of coupling factor complex as well as accumulation of the radicals in the thylakoid membranes after thermal pretreatment.展开更多
Chemical looping combustion (CLC) and chemical looping reforming (CLR) are innovative technologies for clean and efficient hydrocarbon conversion into power, fuels, and chemicals through cyclic redox reac- tions. ...Chemical looping combustion (CLC) and chemical looping reforming (CLR) are innovative technologies for clean and efficient hydrocarbon conversion into power, fuels, and chemicals through cyclic redox reac- tions. Metal oxide materials play an essential role in the chemical looping redox processes. During reduc- tion, the oxygen carriers donate the required amount of oxygen ions for hydrocarbon conversion and product synthesis. In the oxidation step, the depleted metal oxide oxygen carriers are replenished with molecular oxygen from the air while heat is released. In recent years, there have been significant advances in oxygen carrier materials for various chemical looping applications. Among these metal oxide materials, iron-based oxygen carriers are attractive due to their high oxygen-carrying capacity, cost ben- efits, and versatility in applications for chemical looping reactions. Their reactivity can also be enhanced via structural design and modification. This review discusses recent advances in the development of oxy- gen carrier materials and the mechanisms of hydrocarbon conversion over these materials. These advances will facilitate the development of oxygen carrier materials for more efficient chemical looping technology applications.展开更多
The direct synthesis of C2 hydrocarbons (ethylene, acetylene and ethane) from methane is one of the most important task in C1 chemistry. Higher conversion of methane and selectivity to C2 hydrocarbons can be real-iz...The direct synthesis of C2 hydrocarbons (ethylene, acetylene and ethane) from methane is one of the most important task in C1 chemistry. Higher conversion of methane and selectivity to C2 hydrocarbons can be real-ized through plasma reaction. In order to explore the reaction process and mechanism, the possible reaction paths (1)—(4) were proposed on coupling reaction of methane through plasma and studied theoretically using semi-PM3 method [PM3 is parameterization method of modified neglect of diatomic overlap (MNDO)] including determining the transition state, calculating the activation energy and thermodynamic state functions and analyzing the bond or-der and intrinsic reaction coordinate. The reaction heat results indicate that the reactions (2) and (4) are exothermic, while reactions of (1) and (3) are endothermic. The activation energy results show that activation energy for reac-tions (1) and (2) was much lower than that of reaction paths (3) and (4). Therefore, paths (1) and (2) is the favorable reaction path energetically. More interestingly by comparing the intrinsic reaction coordinated (IRC) of the reaction paths (1) and (2), it is found that the variations of bond lengths in reaction path (1) has a crucial effect on the poten-tial energy, while in reaction path (2), the adjustment of the system geometry also contributes to the whole potential energy of the system.展开更多
When the motion of a particle is constrained, excess terms exist using hermitian form of Cartesian momentum pi (i=1, 2,3) in usual kinetic energy (1/2/μ)∑ pi^2 , and the correct kinetic energy turns out to be (...When the motion of a particle is constrained, excess terms exist using hermitian form of Cartesian momentum pi (i=1, 2,3) in usual kinetic energy (1/2/μ)∑ pi^2 , and the correct kinetic energy turns out to be (1/2μ) ∑1/ fipi f ipi, where fi are dummy factors in classical mechanics and nontrivial in quantum mechanics. In this paper the explicit form of the dummy functions fi is given for a charged rigid planar rotator in the uniform magnetic field with different gauge chosen. Under different gauges, we have different sets of dummy factors. It means that these factors do not have direct observable effect.展开更多
Valeo, involved in engine cooling fan system design for many years, is interested in noise prediction tools for axial fans. Thus, this paper describes a two-part study of tonal noise computation. The first part deals ...Valeo, involved in engine cooling fan system design for many years, is interested in noise prediction tools for axial fans. Thus, this paper describes a two-part study of tonal noise computation. The first part deals with the prediction of tonal noise using analytical models. As for the second part, it describes a hybrid approach for predicting tonal noise where the sources are extracted from an Unsteady Reynolds-Averaged Naviers-Stocks (URANS) simulation and then propagated into the far, free field using the Ffowcs Williams and Hawkings' acoustic analogy. The computational domain is meshed with 46 million polyhedral elements and the simulation takes into account the exact geometry of the rotor blades, the stator blades and the shroud. The results from the first part show that analytical models can be used for comparisons between different fan geometries, but are unable to provide accurate noise predictions compared to experimental results. The simulation shows non-periodic blade loading over a whole fan revolution, and different blade loading between the blades. This introduces some bias in the assessment of the acoustic performance of the fan. Overall, the results from the hybrid method are in accordance with the experimental results.展开更多
A novel twin-rotor piston engine (TRPE) mechanism with high volumetric output and power density was introduced. This new engine comprises an energy conversion system and a differential velocity drive mechanism (DVD...A novel twin-rotor piston engine (TRPE) mechanism with high volumetric output and power density was introduced. This new engine comprises an energy conversion system and a differential velocity drive mechanism (DVDM). Two special geared four-bar mechanisms, DVDM-1 and DVDM-2, were utilized and compared. Based on the closed loop vector method, a mathematical model for position, velocity, and acceleration of the two mechanisms was established. Numerical examples illustrate that the kinematic characteristics were presented. Expression of the displacement and compression ratio of the two engine mechanisms were derived and compared. It is concluded that both DVDM-1 and DVDM-2 adopted in the proposed TRPE with six vane pistons create thirty-six power strokes per revolution of the output shaft, and the summation of two angles covered by each rocker is always 2x/N as the output shaft rotates an angle of x/N. In DVDM-1, the span angle of a vane piston should be designed to be 10.2°, and the compression ratio should be equal to 10; in DVDM-2, the span angle of a vane piston should be designed to be 10.6°, and the compression ratio should be equal to 4.3.展开更多
Structural analogues of bicyclic peroxides belonging to the G factors family have been synthesised under Mannieh type conditions, followed by an autoxidation step furnishing exclusively the peroxide. As electron trans...Structural analogues of bicyclic peroxides belonging to the G factors family have been synthesised under Mannieh type conditions, followed by an autoxidation step furnishing exclusively the peroxide. As electron transfer from heine or free iron to peroxide is the first step in the mode of action ofartemisinin -like compound, Fe(fl) induced reduction was studied and the reactivity of the intermediate C-centered radical formed was correlated to the antimalarial activity. Dissociative electron transfer was studied by electrochemistry and allowed the evaluation of the standard reduction potentials and other pertinent thermochemical information. These bicyclic peroxides were evaluated in vitro against Plasmodium falciparum and revealed moderate to good activity.展开更多
文摘A twin ovary mutant derived from the doubled haploid (DH) progeny of a cross,02428/Gui 630, was presumably related to the transposition of some transposable elements. Up to date, all reported the active transposable elements in rice (Oryza sativa L.) are copia like retrotransposons. In the present study, the reverse transcriptase domains of copia like retrotransposons were amplified from the total DNA isolated from the mutant plants with the degenerated oligonucleotide primers for the domain. Three cloned insert DNAs, R33 1, R33 4 and R33 8, representing putative different copia like retrotransposons were screened out. Two of them displayed high polymorphism between “Zhaiyeqing 8” and “Jingxi 17”. Nine of the polymorphic bands were mapped on seven rice chromosomes. Sequencing analysis revealed that stop codons frequently occur in the sequence of R33 8, while both R33 1 and R33 4 contain a continuous coding region for 81 putative amino acid residues. No significant variation in hybridization patterns was found between indica and japonica rice or among 26 varieties of indica rice when R33 1 was used as a probe. Nevertheless, when R33 4 was used as a probe, high polymorphisms were detected both between indica and japonica rice and among 26 indica varieties, implying that this element might still be active in rice genomes.
文摘Characterization of energy-transduction on die chloroplast thylakoid membranes from spinach (Spinacia oleracca L.) after thermal pretreatment was investigated. The related reactions of energy-transduction in chloroplasts were seriously affected by thermal pretreatment. The results were obtained as following: (1) The rate of cyclic photophosphorylation declined when the pretreatment temperature increased in the range of 25 to 45 degreesC. (2) The thermal pretreatment led to a decrease of the activity of thylakoid membrane-bounded ATPase. (3) Proton uptake of chloroplasts acid the fluorescence quenching of 9-aminoacridine (9-AA) in thylakoid membrane decreased after the thermal pretreatment, but addition of dicyclohexylcarbodiimide (DCCD) could partially restore the fluorescence quenching of 9-AA. (4) Both the rates of fast phase in electrochroism absorption change at 515 nm and the millisecond delayed light emission (ms-DLE) of chloroplast showed a progressive decrease upon raising the temperature of pretreatment. (5) Immunbloting analysis showed that the thermal pretreatment caused the changes of protein content and the electrophoresis mobility of thylakoid membrane-bound ATPase and its alpha -subunit. (6) If the temperature of pretreatment were higher than 33 degreesC, oxygen uptake of PS I -mediated in the samples was rapidly inhibited, but addition of sinapine into the reaction medium could partially restore the ability of oxygen uptake in the samples. These results are briefly discussed in relation to the change of permeability of thylakoid membranes, the dissociation of coupling factor complex as well as accumulation of the radicals in the thylakoid membranes after thermal pretreatment.
文摘Chemical looping combustion (CLC) and chemical looping reforming (CLR) are innovative technologies for clean and efficient hydrocarbon conversion into power, fuels, and chemicals through cyclic redox reac- tions. Metal oxide materials play an essential role in the chemical looping redox processes. During reduc- tion, the oxygen carriers donate the required amount of oxygen ions for hydrocarbon conversion and product synthesis. In the oxidation step, the depleted metal oxide oxygen carriers are replenished with molecular oxygen from the air while heat is released. In recent years, there have been significant advances in oxygen carrier materials for various chemical looping applications. Among these metal oxide materials, iron-based oxygen carriers are attractive due to their high oxygen-carrying capacity, cost ben- efits, and versatility in applications for chemical looping reactions. Their reactivity can also be enhanced via structural design and modification. This review discusses recent advances in the development of oxy- gen carrier materials and the mechanisms of hydrocarbon conversion over these materials. These advances will facilitate the development of oxygen carrier materials for more efficient chemical looping technology applications.
基金Supported by the National Natural Science Foundation of China (No.20606023).
文摘The direct synthesis of C2 hydrocarbons (ethylene, acetylene and ethane) from methane is one of the most important task in C1 chemistry. Higher conversion of methane and selectivity to C2 hydrocarbons can be real-ized through plasma reaction. In order to explore the reaction process and mechanism, the possible reaction paths (1)—(4) were proposed on coupling reaction of methane through plasma and studied theoretically using semi-PM3 method [PM3 is parameterization method of modified neglect of diatomic overlap (MNDO)] including determining the transition state, calculating the activation energy and thermodynamic state functions and analyzing the bond or-der and intrinsic reaction coordinate. The reaction heat results indicate that the reactions (2) and (4) are exothermic, while reactions of (1) and (3) are endothermic. The activation energy results show that activation energy for reac-tions (1) and (2) was much lower than that of reaction paths (3) and (4). Therefore, paths (1) and (2) is the favorable reaction path energetically. More interestingly by comparing the intrinsic reaction coordinated (IRC) of the reaction paths (1) and (2), it is found that the variations of bond lengths in reaction path (1) has a crucial effect on the poten-tial energy, while in reaction path (2), the adjustment of the system geometry also contributes to the whole potential energy of the system.
基金The project supported by the Program for New Century Excellent Talents in Universities, Ministry of Education, and the Key Teaching Reform Program of Hunan Province
文摘When the motion of a particle is constrained, excess terms exist using hermitian form of Cartesian momentum pi (i=1, 2,3) in usual kinetic energy (1/2/μ)∑ pi^2 , and the correct kinetic energy turns out to be (1/2μ) ∑1/ fipi f ipi, where fi are dummy factors in classical mechanics and nontrivial in quantum mechanics. In this paper the explicit form of the dummy functions fi is given for a charged rigid planar rotator in the uniform magnetic field with different gauge chosen. Under different gauges, we have different sets of dummy factors. It means that these factors do not have direct observable effect.
文摘Valeo, involved in engine cooling fan system design for many years, is interested in noise prediction tools for axial fans. Thus, this paper describes a two-part study of tonal noise computation. The first part deals with the prediction of tonal noise using analytical models. As for the second part, it describes a hybrid approach for predicting tonal noise where the sources are extracted from an Unsteady Reynolds-Averaged Naviers-Stocks (URANS) simulation and then propagated into the far, free field using the Ffowcs Williams and Hawkings' acoustic analogy. The computational domain is meshed with 46 million polyhedral elements and the simulation takes into account the exact geometry of the rotor blades, the stator blades and the shroud. The results from the first part show that analytical models can be used for comparisons between different fan geometries, but are unable to provide accurate noise predictions compared to experimental results. The simulation shows non-periodic blade loading over a whole fan revolution, and different blade loading between the blades. This introduces some bias in the assessment of the acoustic performance of the fan. Overall, the results from the hybrid method are in accordance with the experimental results.
基金Project(7131109) supported the by National Defense Pre-research Foundation of ChinaProject(50975278) supported by National the Natural Science Foundation of China
文摘A novel twin-rotor piston engine (TRPE) mechanism with high volumetric output and power density was introduced. This new engine comprises an energy conversion system and a differential velocity drive mechanism (DVDM). Two special geared four-bar mechanisms, DVDM-1 and DVDM-2, were utilized and compared. Based on the closed loop vector method, a mathematical model for position, velocity, and acceleration of the two mechanisms was established. Numerical examples illustrate that the kinematic characteristics were presented. Expression of the displacement and compression ratio of the two engine mechanisms were derived and compared. It is concluded that both DVDM-1 and DVDM-2 adopted in the proposed TRPE with six vane pistons create thirty-six power strokes per revolution of the output shaft, and the summation of two angles covered by each rocker is always 2x/N as the output shaft rotates an angle of x/N. In DVDM-1, the span angle of a vane piston should be designed to be 10.2°, and the compression ratio should be equal to 10; in DVDM-2, the span angle of a vane piston should be designed to be 10.6°, and the compression ratio should be equal to 4.3.
文摘Structural analogues of bicyclic peroxides belonging to the G factors family have been synthesised under Mannieh type conditions, followed by an autoxidation step furnishing exclusively the peroxide. As electron transfer from heine or free iron to peroxide is the first step in the mode of action ofartemisinin -like compound, Fe(fl) induced reduction was studied and the reactivity of the intermediate C-centered radical formed was correlated to the antimalarial activity. Dissociative electron transfer was studied by electrochemistry and allowed the evaluation of the standard reduction potentials and other pertinent thermochemical information. These bicyclic peroxides were evaluated in vitro against Plasmodium falciparum and revealed moderate to good activity.