[Objective] This study aimed to perform the bioinformatics analysis of Zinc transporter （ZnT） from Baoding Alfalfa. [Method] Based on the amino acid sequence, the physical and chemical properties, hydrophilicity/hyd...[Objective] This study aimed to perform the bioinformatics analysis of Zinc transporter （ZnT） from Baoding Alfalfa. [Method] Based on the amino acid sequence, the physical and chemical properties, hydrophilicity/hydrophobicity, secondary structure of ZnT from Baoding alfalfa were predicted by a series of bioinformatics software. And the transmembrane domains were predicted by using different online tools. [Result] ZnT is a hydrophobic protein containing 408 amino acids with the theoretical pl of 5.94, and it has 7 potential transmembrane hydrophobic regions. In the sec- ondary structure, co-helix （Hh） accounted for 48.04%, extended strand （Ee） for 9.56%, random coil （Cc） for 42.40%, which was accored with the characteristic of transmembrane protein. [Conclusion] mZnT is a member of CDF family, responsible for transporting Zn^2＋ out of the cell membrane to reduce the concentration and toxicity of Zn^2＋.展开更多
AIM: To analyze the metastasis-related proteins in hepatocellular carcinoma (HCC) and discover the biomarker candidates for diagnosis and therapeutic intervention of HCC metastasis with bioinformatics tools. METHODS: ...AIM: To analyze the metastasis-related proteins in hepatocellular carcinoma (HCC) and discover the biomarker candidates for diagnosis and therapeutic intervention of HCC metastasis with bioinformatics tools. METHODS: Metastasis-related proteins were determined by stable isotope labeling and MS analysis and analyzed with bioinformatics resources, including Phobius, Kyoto encyclopedia of genes and genomes (KEGG), online mendelian inheritance in man (OMIM) and human protein reference database (HPRD). RESULTS: All the metastasis-related proteins were linked to 83 pathways in KEGG, including MAPK and p53 signal pathways. Protein-protein interaction network showed that all the metastasis-related proteins were categorized into 19 function groups, including cell cycle, apoptosis and signal transduction. OMIM analysis linked these proteins to 186 OMIM entries. CONCLUSION: Metastasis-related proteins provide HCC cells with biological advantages in cell proliferation,migration and angiogenesis, and facilitate metastasis of HCC cells. The bird's eye view can reveal a global characteristic of metastasis-related proteins and many differentially expressed proteins can be identified as candidates for diagnosis and treatment of HCC.展开更多
Focusing on the global food security and analyzing the challenges now confronting human being of global climate change,sustainable development of agriculture,energy and environment,plant scientists should take the soc...Focusing on the global food security and analyzing the challenges now confronting human being of global climate change,sustainable development of agriculture,energy and environment,plant scientists should take the social responsibility and play the role in solving the global problems like food security from the developmental status and potential of plant science and technology. Moreover,by working with agronomists, ecologists and environmental experts,plant scientists do provide novel ideas and technologies for developing low carbon agriculture,for breeding new crop varieties with high yield and quality and stress resistance,for developing new type of energy plants,for supplying healthy foods and drugs,reasonably using land and water resources,and for ameliorating environment,making better contribution to the sustainable development of the globe and human being ourselves.展开更多
基金Supported by the General Project of Qujing Normal University(2010MS007)~~
文摘[Objective] This study aimed to perform the bioinformatics analysis of Zinc transporter （ZnT） from Baoding Alfalfa. [Method] Based on the amino acid sequence, the physical and chemical properties, hydrophilicity/hydrophobicity, secondary structure of ZnT from Baoding alfalfa were predicted by a series of bioinformatics software. And the transmembrane domains were predicted by using different online tools. [Result] ZnT is a hydrophobic protein containing 408 amino acids with the theoretical pl of 5.94, and it has 7 potential transmembrane hydrophobic regions. In the sec- ondary structure, co-helix （Hh） accounted for 48.04%, extended strand （Ee） for 9.56%, random coil （Cc） for 42.40%, which was accored with the characteristic of transmembrane protein. [Conclusion] mZnT is a member of CDF family, responsible for transporting Zn^2＋ out of the cell membrane to reduce the concentration and toxicity of Zn^2＋.
基金National Basic Research Priorities Program No. 2001CB510202National Science and Technology Key Project No. 2002BAC11A11 and 2004BA711A19+2 种基金National Natural Science Foundation of China No. 20328508National High Technology Research Developing Program No. 02BAC11A11Shanghai Science and Technology Development Program No. 03DZ14024
文摘AIM: To analyze the metastasis-related proteins in hepatocellular carcinoma (HCC) and discover the biomarker candidates for diagnosis and therapeutic intervention of HCC metastasis with bioinformatics tools. METHODS: Metastasis-related proteins were determined by stable isotope labeling and MS analysis and analyzed with bioinformatics resources, including Phobius, Kyoto encyclopedia of genes and genomes (KEGG), online mendelian inheritance in man (OMIM) and human protein reference database (HPRD). RESULTS: All the metastasis-related proteins were linked to 83 pathways in KEGG, including MAPK and p53 signal pathways. Protein-protein interaction network showed that all the metastasis-related proteins were categorized into 19 function groups, including cell cycle, apoptosis and signal transduction. OMIM analysis linked these proteins to 186 OMIM entries. CONCLUSION: Metastasis-related proteins provide HCC cells with biological advantages in cell proliferation,migration and angiogenesis, and facilitate metastasis of HCC cells. The bird's eye view can reveal a global characteristic of metastasis-related proteins and many differentially expressed proteins can be identified as candidates for diagnosis and treatment of HCC.
文摘Focusing on the global food security and analyzing the challenges now confronting human being of global climate change,sustainable development of agriculture,energy and environment,plant scientists should take the social responsibility and play the role in solving the global problems like food security from the developmental status and potential of plant science and technology. Moreover,by working with agronomists, ecologists and environmental experts,plant scientists do provide novel ideas and technologies for developing low carbon agriculture,for breeding new crop varieties with high yield and quality and stress resistance,for developing new type of energy plants,for supplying healthy foods and drugs,reasonably using land and water resources,and for ameliorating environment,making better contribution to the sustainable development of the globe and human being ourselves.
