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局灶性脑缺血大鼠CREB1活性调节转导子的相关研究
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作者 刘玲玲 焦清海 +3 位作者 赵晓梅 马兰 陈惠英 王力娜 《脑与神经疾病杂志》 2014年第1期46-49,共4页
目的探讨磷酸化cAMP反应元件结合蛋白(p-CREB)、CREB1活性调节转导子(TORC1)和脑源性神经影响因子(BDNF)在局灶性脑缺血大鼠脑皮质中的表达规律及意义。方法用线栓法分别制作雄性SD大鼠右侧大脑中动脉闭塞(MCAO)后3 h、6 h、12 h、24 h... 目的探讨磷酸化cAMP反应元件结合蛋白(p-CREB)、CREB1活性调节转导子(TORC1)和脑源性神经影响因子(BDNF)在局灶性脑缺血大鼠脑皮质中的表达规律及意义。方法用线栓法分别制作雄性SD大鼠右侧大脑中动脉闭塞(MCAO)后3 h、6 h、12 h、24 h、48 h及72h模型,应用蛋白印迹法分别检测TORC1、BDNF和p-CREB在局灶性脑缺血皮质的蛋白表达水平,用聚合酶链反应技术检测TORC1和BDNF mRNA的相对表达水平。结果与正常组相比,TORC1的核累积和细胞表达的两个高峰为术后3 h和48 h,而p-CREB和BDNF的两个高峰分别为3 h和72 h,差异有统计学意义(P<0.05)。结论 TORC1可能在大鼠局灶性脑缺血后48 h前参与促进了顶叶皮质神经元细胞CREB/BDNF通路的活化,对皮质神经元具有保护作用。 展开更多
关键词 CREB1活性调节转导子 脑源性神经影响因子 环单磷酸腺苷反应元件结合蛋白 大脑中动脉闭塞
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支气管哮喘豚鼠气道上皮细胞信号转导子与转录活化因子1表达及其对气道炎症的调控 被引量:13
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作者 李国平 熊瑛 +1 位作者 刘志刚 钟南山 《中华结核和呼吸杂志》 CAS CSCD 北大核心 2004年第5期306-310,共5页
目的 研究信号转导子与转录活化子 1(STAT 1)在支气管哮喘 (简称哮喘 )中的表达及STAT 1对哮喘气道炎症调控 ,以探讨STAT 1信号传导途径在哮喘发病机制中的作用。方法 4 8只豚鼠随机分为正常对照组 (A组 ,8只 )、哮喘组 (B组 )和地塞... 目的 研究信号转导子与转录活化子 1(STAT 1)在支气管哮喘 (简称哮喘 )中的表达及STAT 1对哮喘气道炎症调控 ,以探讨STAT 1信号传导途径在哮喘发病机制中的作用。方法 4 8只豚鼠随机分为正常对照组 (A组 ,8只 )、哮喘组 (B组 )和地塞米松预防组 (C组 ,8只 ) ,哮喘组又随机分为B1、B2 、B3 、B4组 (每组各 8只 )。B组与C组用 10 %卵蛋白 (OVA)腹腔注射与雾化吸入诱发豚鼠哮喘发作 ,在激发后 0h、2 4h、72h、5d通过免疫组化测定豚鼠哮喘模型肺组织中STAT 1与胞间黏附分子 1(ICAM 1)表达 ,免疫印迹检测STAT 1磷酸化 ,测定豚鼠哮喘模型支气管肺泡灌洗液 (BALF)中细胞总数、嗜酸粒细胞计数 ,用酶联免疫吸附法检测γ干扰素 (IFN γ)的浓度。结果 A组气道上皮细胞STAT 1与ICAM 1积分吸光度分别为 13± 7、2 1± 8,而哮喘组 (B1~ 4组 )豚鼠在激发各个时间点 (0h、2 4h、72h、5d)其气道上皮细胞STAT 1与ICMA 1的积分吸光度分别为 5 7± 9、136± 14、95± 2 1、6 7±30 ;75± 10、16 6± 17、113± 14、87± 2 1。B1~ 4组与A组比较差异有显著性 (P <0 0 1) ;气道上皮细胞STAT 1积分吸光度变化与嗜酸粒细胞动态变化呈正相关 (r=0 6 5 2 ,P <0 0 1) ;STAT 1积分吸光度与ICMA 1积分吸光度呈正相关 (r=0 5 展开更多
关键词 支气管哮喘 豚鼠 气道上皮细胞信号转导子 转录活化因子1 表达 气道炎症 调控
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盐诱导激酶对TORC-CREB复合体的调节及其与高血压、糖尿病的关系 被引量:2
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作者 刘瑜 李静 +1 位作者 金大庆 唐向东 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2009年第3期274-279,共6页
环腺苷酸反应元件结合蛋白(cyclic AMP responsiveelement-binding protein,CREB)是受cAMP和Ca2+共同激活的转录因子,其目的基因产物涉及广泛的生理过程,如细胞增殖与存活、糖与脂类代谢、类固醇激素合成、学习与记忆等.新近发现的CREB... 环腺苷酸反应元件结合蛋白(cyclic AMP responsiveelement-binding protein,CREB)是受cAMP和Ca2+共同激活的转录因子,其目的基因产物涉及广泛的生理过程,如细胞增殖与存活、糖与脂类代谢、类固醇激素合成、学习与记忆等.新近发现的CREB活性调节转导子(transducer of regulated CREB activity,TORC)通过核-质穿梭调节CREB的活性而控制目的基因的转录与表达.盐诱导激酶(salt-inducible kinase,SIK)是一组丝氨酸/苏氨酸激酶,包含SIK1、SIK2和SIK3.这些蛋白激酶通过影响TORC的磷酸化水平,改变其在核-质中的分布,间接影响CREB目的基因的转录与表达.在某些器官与组织中,SIK(SIK1)也是CREB目的基因之一,因此SIK与TORC-CREB复合体形成一个完整的负反馈调节环路.TORC-CREB复合体广泛存在于多种器官与组织,如胰岛β-细胞、肝脏、肾上腺皮质和骨骼肌中,与胰岛β-细胞存活、肝脏糖异生、类固醇激素合成、骨骼肌线粒体增生与脂肪酸β氧化密切相关.将重点讨论SIK对TORC-CREB复合体的反馈调节及其与高血压、糖尿病发生的关系. 展开更多
关键词 环腺苷酸反应元件结合蛋白(CREB) CREB活性调节转导子(TORC) 盐诱导激酶(SIK)
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CD36介导氧化低密度脂蛋白对巨噬细胞内质网应激的诱导作用 被引量:1
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作者 姚树桐 桑慧 +5 位作者 杨娜娜 康莉 田华 张颖 宋国华 秦树存 《中国病理生理杂志》 CAS CSCD 北大核心 2010年第A10期1971-1971,共1页
关键词 CD36 巨噬细胞 phospho 清道夫受体 油红 磷酸化水平 RAW 免疫印迹法 转导子 体外培养
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丹参酮ⅡA对局灶性脑缺血大鼠急性期脑保护作用的相关研究 被引量:3
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作者 刘玲玲 刘红 +4 位作者 尹君玲 陈惠英 刘晓蕾 焦清海 武一平 《中国卒中杂志》 2014年第1期20-25,共6页
目的探讨丹参酮ⅡA(TashinoneⅡA,TSA)对局灶性脑缺血急性期大鼠顶叶皮质磷酸化环腺苷酸反应元件结合蛋白(phosphorylated cyclic adenosine monophosphate response element binding protein,p-CREB)和CREB1活性调节转导子(transducers... 目的探讨丹参酮ⅡA(TashinoneⅡA,TSA)对局灶性脑缺血急性期大鼠顶叶皮质磷酸化环腺苷酸反应元件结合蛋白(phosphorylated cyclic adenosine monophosphate response element binding protein,p-CREB)和CREB1活性调节转导子(transducers of regulated CREB1,TORC1)的蛋白表达和脑梗死体积的影响。方法采用成年健康雄性SD大鼠,随机分为假手术组、对照组、小剂量TSA组和大剂量TSA组,每组各12只。用线栓法制作右侧大脑中动脉闭塞模型,采用Longa评分判断动物模型成功与否。小剂量TSA组和大剂量TSA组在造模后立刻腹腔注射TSA溶液,剂量分别为:10 mg/kg及20 mg/kg,假手术组及对照组分别腹腔注射等量的生理盐水。造模成功后24 h将大鼠处死,采用2%2,3,5-三苯基四唑氮红染色计算梗死体积,应用Western blotting法检测各组手术侧顶叶皮质梗死灶神经细胞核TORC1和细胞核p-CREB以及细胞TORC1的蛋白表达。结果与对照组相比,大剂量TSA组大鼠脑组织梗死体积明显降低(P=0.004),细胞核TORC1表达显著增加(P<0.001);与对照组相比,小剂量TSA组大鼠脑组织梗死体积减小,无显著差异(P=0.148),细胞核TORC1蛋白表达升高,也无显著差异(P=0.083),细胞核p-CREB和细胞TORC1的蛋白表达水平无论在大剂量组(P均<0.001),还是小剂量组(P分别为0.002和0.001)均显著升高。结论 TSA可以上调脑梗死大鼠病灶侧顶叶皮质神经细胞TORC1-CREB信号通路的表达。 展开更多
关键词 环腺苷酸反应元件结合蛋白活性调节转导子 环腺苷酸反应元件结合蛋白 丹参酮 IIA 大脑中动脉闭塞
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STAT3通过上调Twist基因表达从而诱导肺腺癌细胞上皮-间质转化 被引量:1
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作者 李志伟 王凤云 +4 位作者 张倩倩 李丁楠 张纯慧 燕飞虎 张艳桥 《实用肿瘤学杂志》 CAS 2014年第4期310-314,共5页
目的探讨信号转导子与转录激活子3(STAT3)基因在肺腺癌细胞上皮-间质转化(Epi.thelial-mesenchymal transition,EMT)过程中的作用。方法应用STAT3-siRNA转染人肺腺癌A549细胞,下调STAT3基因表达。转染后观察细胞形态学变化,通过... 目的探讨信号转导子与转录激活子3(STAT3)基因在肺腺癌细胞上皮-间质转化(Epi.thelial-mesenchymal transition,EMT)过程中的作用。方法应用STAT3-siRNA转染人肺腺癌A549细胞,下调STAT3基因表达。转染后观察细胞形态学变化,通过Westernblot检测STAT3和Twist蛋白的表达变化。同时应用Transwell法和划痕实验检测细胞的侵袭和迁移能力。并应用免疫组化法验证STAT3和Twist蛋白在肺腺癌组织中表达的相关性。结果STAT3-siRNA转染人肺腺癌A549细胞后,STAT3基因表达下降,同时降低Twist的表达,并且降低肺癌细胞的增殖、侵袭和迁移能力,在肺腺癌组织中我们也观察到随着分化程度升高,两个蛋白在肺癌组织中的的表达均逐渐降低,并呈正相关。结论STAT3基因通过调节Twist的表达,参与EMT过程,从而在肺癌进展中发挥重要作用。 展开更多
关键词 肺腺癌 转导子与转录激活子3 TWIST 上皮-间质转化
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RNAi技术沉默STAT1基因对哮喘小鼠气道炎症的影响 被引量:4
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作者 刘春凤 任磊 熊瑛 《泸州医学院学报》 2015年第1期7-10,共4页
目的:探讨RNA干扰(RNA interference,RNAi)技术沉默转导子与转录活化子1(signal transducer and activator of transcription 1,STAT1)基因对支气管哮喘小鼠气道炎症的影响。方法 :将32只4周龄BALB/c雌鼠随机分为正常组、PBS组、空质粒... 目的:探讨RNA干扰(RNA interference,RNAi)技术沉默转导子与转录活化子1(signal transducer and activator of transcription 1,STAT1)基因对支气管哮喘小鼠气道炎症的影响。方法 :将32只4周龄BALB/c雌鼠随机分为正常组、PBS组、空质粒(随机片段RNAi)组和si RNA(p GSTAT1-2)组,利用RNAi沉默支气管哮喘小鼠中STAT1基因,采用免疫组织化学检测STAT1蛋白的表达;通过单肺灌洗留取肺泡灌洗液(BALF),应用酶联免疫吸附试验法(ELISA)检测气道炎症介质如γ干扰素(IFN-γ)和白细胞介素5(IL-5)的表达水平。结果 :si RNA组的IFN-γ浓度高于PBS组、空质粒组(P<0.05),而IL-5的表达水平低于PBS组、空质粒组(P<0.05),二者在PBS组和空质粒组组间均无差异(P>0.05);si RNA组BALF中嗜酸性粒细胞(EOS)数量和IL-5浓度较空质粒组和PBS组中均明显减低,而IFN-γ的浓度增高,IFN-γ与EOS呈负相关,IL-5与EOS呈正相关,si RNA组BALF中白细胞(WBC)数量较空质粒组和PBS组中均明显减低;STAT-1蛋白主要表达于气道上皮细胞,si RNA组STAT-1蛋白表达明显低于PBS组与空质粒组,正常组低于si RNA组、PBS组和空质粒组,有统计学意义(P<0.05)。结论 :si RNA能有效抑制哮喘小鼠气道上皮细胞STAT1蛋白的表达,减轻哮喘小鼠的气道炎症反应。 展开更多
关键词 哮喘 沉默转导子与转录活化子1 RNA干扰.
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Tunneling Electrons Triggered Energy Transfer between Coherently Coupled Donor-Acceptor Molecules
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作者 Huifang Wang 《Chinese Journal of Chemical Physics》 SCIE EI CAS CSCD 2024年第4期497-504,I0093,I0094,共10页
Energy transfer is ubiquitous in natural and artificial lightharvesting systems,and coherent energy transfer,a highly efficient energy transfer process,has been accepted to play a vital role in such systems.However,th... Energy transfer is ubiquitous in natural and artificial lightharvesting systems,and coherent energy transfer,a highly efficient energy transfer process,has been accepted to play a vital role in such systems.However,the energy oscillation of coherent energy transfer is exceedingly difficult to capture because of its evanescence due to the interaction with a thermal environment.Here a microscopic quantum model is used to study the time evolution of electrons triggered energy transfer between coherently coupled donoracceptor molecules in scanning tunneling microscope(STM).A series of topics in the plasmonic nanocavity(PNC)coupled donor-acceptor molecules system are discussed,including resonant and nonresonant coherent energy transfer,dephasing assisted energy transfer,PNC coupling strength dependent energy transfer,Fano resonance of coherently coupled donor-acceptor molecules,and polariton-mediated energy transfer. 展开更多
关键词 Energy transfer Coherent energy transfer Scanning tunneling microcopy induced luminescence Plasmonic nanocavity Quantum master equation DEPHASING Fano resonance Polariton-mediated energy transfer
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缺氧调控JAR细胞Stat3表达及细胞凋亡的研究 被引量:1
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作者 杜尚珂 石瑛 +4 位作者 岳宁 张琳琳 于海洋 贾莉婷 张展 《重庆医学》 CAS 北大核心 2016年第17期2312-2313,2318,共3页
目的探讨缺氧对JAR细胞信号转导子和转录激活子3(Stat3)和磷酸化Stat3(p-Stat3)蛋白表达及细胞凋亡的影响。方法在缺氧条件下培养JAR细胞,通过蛋白免疫印迹法(Western blot)检测Stat3和p-Stat3在缺氧前后蛋白表达的差异,采用流式细胞术... 目的探讨缺氧对JAR细胞信号转导子和转录激活子3(Stat3)和磷酸化Stat3(p-Stat3)蛋白表达及细胞凋亡的影响。方法在缺氧条件下培养JAR细胞,通过蛋白免疫印迹法(Western blot)检测Stat3和p-Stat3在缺氧前后蛋白表达的差异,采用流式细胞术检测细胞凋亡水平。结果缺氧培养JAR细胞48h后,细胞形态发生异常改变,Stat3和p-Stat3蛋白表达水平均明显降低(P<0.05),细胞凋亡水平明显增加(P<0.05)。结论缺氧使JAR细胞Stat3和p-Stat3蛋白表达水平降低,凋亡增加。 展开更多
关键词 细胞信号转导子和转录激活子3 缺氧 滋养细胞 凋亡
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MODELING OF SCG ROTOR DRIFT AND MEASURING SCHEME 被引量:1
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作者 汤继强 房建成 张延顺 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2007年第1期17-24,共8页
Based on micro-displacement measurement principles of the spherical differential capacitance sensor, the relationship between the capacitance variation and the micro-displacement of each pair of detecting electrodes f... Based on micro-displacement measurement principles of the spherical differential capacitance sensor, the relationship between the capacitance variation and the micro-displacement of each pair of detecting electrodes for the superconducting gyroscope (SCG) with eight detecting electrodes is analyzed. The model of the SCG rotor drift is established through dimensionless processing, linearization within micro-displacement and the least-square approach. Both the measurement scheme of the SCG rotor drift based on the model and its parameter relationship are presented. To guarantee the potential of the suspension rotor to be zero, the distributing scheme of four pairs of detecting electrodes is presented. The scheme can measure the magnitude and the direction of the rotor drift. The negative factors for affecting the measurement precision of .the SCG rotor drift and simulation results of the total effects are given. Simulation results show that the distributing capacitance of these differential capacitance sensors, the zero potential of the rotor and the model error are the major negative factors. The methods for eliminating those negative factors and the application range of the model are given. The model ensures the relationship between the output voltage and the rotor drift be linear. 展开更多
关键词 superconducting gyroscope suspension rotor DRIFT spherical differential capacitance MICRO-DISPLACEMENT
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Effect of linear carboxylic ester on low temperature performance of LiMn_2O_4-graphite cells 被引量:3
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作者 洪树 李劼 +2 位作者 王冠超 张治安 赖延清 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第1期206-210,共5页
To improve the low-temperature performances of Li-ion cells, three types of linear carboxylic ester-based electrolyte, such as EC/EMC/EA(1:1:2, mass ratio), EC/EMC/EP(1:1:2, mass ratio) and EC/EMC/EB(1:1:2,... To improve the low-temperature performances of Li-ion cells, three types of linear carboxylic ester-based electrolyte, such as EC/EMC/EA(1:1:2, mass ratio), EC/EMC/EP(1:1:2, mass ratio) and EC/EMC/EB(1:1:2, mass ratio), were prepared to substitute for industrial electrolyte(EC/EMC/DMC). Then, 18650-type Li Mn2O4-graphite cells(nominal capacity of 1150 mA ·h) were assembled and studied. Results show that the cells containing three types of electrolyte are able to undertake 5C discharging current with above 93% capacity retention at-20 °C. Electrochemical impedance spectra show that the discharge capacity fading of Li-ion cells at low temperature is mainly ascribed to the charge transfer resistance increasing with temperature decreasing. In comparison, the cells containing electrolyte of 1.0 mol/L LiPF6 in EC/EMC/EA(1:1:2, mass ratio) have the highest capacity retention of 90% at-40 °C and 44.41% at-60 °C, due to its lowest charge-transfer resistance. 展开更多
关键词 Li-ion cells low temperature performance ELECTROLYTE linear carboxylic ester ionic conductivity charge-transfer resistance
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Differential Interference Angle of Collision-induced Rotational Energy Transfer in Na_2(A^1∑_u^+,v=8-b^3∏_(0u),v=14)-Na System
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作者 李健 李永庆 +1 位作者 王伟丽 马凤才 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 北大核心 2006年第6期469-472,共4页
Here wc report calculation of the differential interference angles (including b≤p gild b≥p ) for singlet-triplet mixed states of Na2(A^1∑u^+,ν=8-b^3∏0u,ν=14) system in collision with Na, in order to study t... Here wc report calculation of the differential interference angles (including b≤p gild b≥p ) for singlet-triplet mixed states of Na2(A^1∑u^+,ν=8-b^3∏0u,ν=14) system in collision with Na, in order to study the collision- induced quantum interference on rotational energy transfer in an atom-diatom system. The calculation is based on the first-order Born approximation of time-dependent perturbation theory, and the anisotropic Lennard-Jones intcraction potentials are also employed, The relationships between differential interference angle and impact parameter, including collision diameter and velocity, are obtained, 展开更多
关键词 Rotational energy transfer Collision induced quantum interference Interference angle
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Cloning of Brassica napus EIN3 Gene and Its Expression Induced by Sclerotinia sclerotiorum 被引量:4
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作者 许李明 黄军艳 +2 位作者 刘学群 覃瑞 刘胜毅 《Agricultural Science & Technology》 CAS 2009年第2期33-36,共4页
[Objective] The study was to investigate roles of Brassica napus EINB in ( BnEIN3 ) resistance to Sclerotinia sclerotiorum. [ Methods] Genomic PCR and RT-PCR were carded out to isolate genomic DNA and cDNA sequences... [Objective] The study was to investigate roles of Brassica napus EINB in ( BnEIN3 ) resistance to Sclerotinia sclerotiorum. [ Methods] Genomic PCR and RT-PCR were carded out to isolate genomic DNA and cDNA sequences of BnEIN3 from oilseed rape, based on the highly conserved region of EIN3 gene from Arabidopsis thaliana and the homologous sequences of oilseed rape ESTs. Expression levels of BnEIN3 were detected in three varieties of oilseed rape inoculated with S. sclerotiorum by real-time quantitative PCR.[Results] A 1 947 bp DNA fragment was obtained from oilseed rape. The fragment shared 82% identity to A. thaliana EIIV3, encoded 614 amino acids containing an EIN3 domain, and was named as BnEIN3. Real-time PCR results showed that expression patterns of BnEIN3 were drastically different in different varieties. In highly resistant oilseed rape variety D083, BnEIN3 expression level was significantly increased 72 h after S. sclerotiorum inoculation whereas in middle resistant and susceptible varieties Zhongshuang 9 and 84039, BnEIN3 expression was suppressed. [ Conclusion ] BnEIIV3 may play an important role in oilseed rape resistance to S. sclerotiorum. 展开更多
关键词 Brassica napus L. EINB RESISTANCE Sclerotinia sclerotiorum Real time PCR
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Collisional Line Assignments and Hyperfine Structure Interpretation in Cs22^3△1g State
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作者 李丹 谢锋 +2 位作者 李丽 Ergin H.Ahmed A.Marjatta Lyyra 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2013年第1期13-19,I0003,共8页
Accurately known energy level structure of the A'∑u+b3 IIu complex of states from a recent global de-perturbation of these states has enabled additional assignments of 140 perturbation facilitated infrared-infrared... Accurately known energy level structure of the A'∑u+b3 IIu complex of states from a recent global de-perturbation of these states has enabled additional assignments of 140 perturbation facilitated infrared-infrared double resonance (PFIIDR) transitions to the 2^3△1g state from collisionally populated intermediate 1 + A Eu levels. Together with the 221 previously observed 2^3△1g←A1∑u+←X1∑g+ Eu X Eg double resonance lines [J. Chem. Phys. 128, 204313 (2008)], molecular constants and the Rydberg-Klein-Rees potential energy curve of the 23△1g state have been recalculated (excluding 54 perturbed levels). The centrifugal distortion constant has been determined and agrees well with the value calculated based on standard empirical formulas. The hyperfine structure of the 23△1g state, which has not resolved in our sub-Doppler excitation spectra of the 23△1g state, has been interpreted with a preliminary simulation. 展开更多
关键词 Cs22^3△1g state Collision-induced energy transfer Hyperfine structure
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Molecular mechanism of TNF signaling and beyond 被引量:25
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作者 Zheng-gangLIU 《Cell Research》 SCIE CAS CSCD 2005年第1期24-27,共4页
Tumor necrosis factor (TNF) is a proinflammatory cytokine that plays a critical role in diverse cellular events, including cell proliferation, differentiation and apoptosis. TNF is also involved in many types of disea... Tumor necrosis factor (TNF) is a proinflammatory cytokine that plays a critical role in diverse cellular events, including cell proliferation, differentiation and apoptosis. TNF is also involved in many types of diseases. In recent years, the molecular mechanisms of TNF functions have been intensively investigated. Studies from many laboratories have demonstrated that the TNF-mediated diverse biological responses are achieved through activating multiple signal- ing pathways. Especially the activation of transcription factors NF-κB and AP-1 plays a critical role in mediating these cellular responses. Several proteins, including FADD, the death domain kinase RIP and the TNF receptor associated factor TRAF2 have been identified as the key effectors of TNF signaling. Recently, we found that the effector mol- ecules of TNF signaling, such as RIP and TRAF2, are also involved in other cellular responses. These finding suggests that RIP and TRAF2 serve a broader role than as just an effector of TNF signaling. 展开更多
关键词 TNF ROS NECROSIS apoptosis JNK.
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Role of transforming growth factor-beta1-smad signal transduction pathway in patients with hepatocellular carcinoma 被引量:26
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作者 Guo-Zhong Ji Xue-Hao Wang +4 位作者 Lin Miao Zheng Liu Ping Zhang Fa-Ming Zhang Jian-Bing Yang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第4期644-648,共5页
AIM: To explore the role of transforming growth factorbeta1 (TGF-β1)-smad signal transduction pathway in patients with hepatocellular carcinoma. METHODS: Thirty-six hepatocellular carcinoma specimens were obtaine... AIM: To explore the role of transforming growth factorbeta1 (TGF-β1)-smad signal transduction pathway in patients with hepatocellular carcinoma. METHODS: Thirty-six hepatocellular carcinoma specimens were obtained from Qidong Liver Cancer Institute and Department of Pathology of the Second Affiliated Hospital of Nanjing Medical University. All primary antibodies (polyclonal antibodies) to TGF-β1, type H Transforming growth factor-beta receptor (TβR-Ⅱ), nuclear factor-kappaB (NF-KB), CD34, smad4 and smad7, secondary antibodies and immunohistochemical kit were purchased from Zhongshan Biotechnology Limited Company (Beijing, China). The expressions of TGF-β1, TβR-Ⅱ, NF- KB, smad4 and smad7 proteins in 36 specimens of hepatocellular carcinoma (HCC) and its adjacent tissue were separately detected by immunohistochemistry to observe the relationship between TGF-β1 and TβR-Ⅱ, between NF-KB and TGF-β1, between smad4 and smad7 and between TGF-β1 or TβR-Ⅱ and microvessel density (MVD). MVD was determined by labelling the vessel endothelial cells with CD34. RESULTS: The expression of TGF-β1, smad7 and MVD was higher in HCC tissue than in adjacent HCC tissue (P〈0.01, P〈0.05,P〈0.01 respectively). The expression of TβR-Ⅱ and smad4 was lower in HCC tissue than in its adjacent tissue (P〈0.01, P〈0.05 respectively). The expression of TGF-β1 protein and NF-KB protein was consistent in HCC tissue. The expression of TGF-β1 and MVD was also consistent in HCC tissue. The expression of TIER- Ⅱ was negatively correlated with that of MVD in HCC tissue. CONCLUSION: The expressions of TGF-IB1, TβR- Ⅱ, NF-KB, smad4 and smad7 in HCC tissue, which are major up and down stream factors of TGF-β1-smad signal transduction pathway, are abnormal. These factors are closely related with NVD and may play an important role in HCC angiogenesis. The inhibitory action of TGF-β1 is weakened in hepatic carcinoma cells because of abnormality of TGF-β1 receptors (such as TIBR- Ⅱ) and postreceptors (such as smad4 and smad7). NF-KB may cause activation and production of TGF-β1. 展开更多
关键词 TGF-β1 TβR-Ⅱ Smad4 Smad7 NF-kB MVD Hepatocellular carcinoma Signal transduction
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Study of signal transduction factors involved in mycoparasitic response of Trichoderma atroviride 被引量:1
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作者 Scala V Zeilinger S +7 位作者 Ambrosino P Brunner K Reithner B Mach R L Woo S L Cristilli M Scala F Lorito M 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2004年第4期451-451,共1页
Numerous Trichoderma spp. are mycoparasites and commercially applied as biological control agents against a large number of plant pathogenic fungi. The mycoparasitic interaction is host-specific and several research s... Numerous Trichoderma spp. are mycoparasites and commercially applied as biological control agents against a large number of plant pathogenic fungi. The mycoparasitic interaction is host-specific and several research strategies have been applied to identify the main genes and compounds involved in the antagonist-plant-pathogen three-way interaction. During mycoparasitism, signals from the host fungus are recognised by Trichoderma, stimulating antifungal activities that are accompanied by morphological changes and the secretion of hydrolytic enzymes and antibiotics. Interestingly some morphological changes appeared highly conserved in the strategy of pathogenicity within the fungal world, i.e. the formation of appressoria as well as the secretion of hydrolytic enzymes seem to be general mechanisms of attack both for plant pathogens and mycoparasitic antagonists. This knowledge is being used to identify receptors and key components of signalling pathways involved in fungus-fungus interaction. For this purpose we have cloned the first genes (tmk1, tga1, tga3) from T. atroviride showing a high similarity to MAP kinase and G protein subunits (see abstract by Zeilinger et al.), which have been found to have an important role in pathogenicity by Magnaporthe grisea. To identify the function and involvement of these factors in mycoparasitism by T. atroviride, tmk1, tga1, tga3 disruptant strains were produced. The knock-out mutants were tested by in vivo biocontrol assays for their ability to inhibit soil and foliar plant pathogens such as Rhizoctonia solani, Pythium ultimum and Botrytis cinerea . Disruption of these genes corresponded to a complete loss of biocontrol ability, suggesting a significant role in mycoparasitism. In particular, it has been suggested that tga3 regulates the expression of chitinase-encoding genes, the secretion of the corresponding enzymes and the process of conidiation. Comparative proteome analysis of wild type and disruptants supported this hypothesis, and indicated many changes in the protein profiles of T. atroviride in different interaction conditions with plants and pathogenic hosts. 展开更多
关键词 PATHOGENICITY MAP kinase G proteins
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Molecular Mechanism of Rice in Response to Salt Stress 被引量:1
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作者 E Zhi-guo ZHANG Li-jing WANG Lei 《Agricultural Science & Technology》 CAS 2011年第6期785-788,共4页
Rice is moderately sensitive to salinity,and the response to salt stress is a complex process,including the perception and transduction of salt stress signal,the activation of specific transcriptional factors and the ... Rice is moderately sensitive to salinity,and the response to salt stress is a complex process,including the perception and transduction of salt stress signal,the activation of specific transcriptional factors and the expression of downstream stress-responsive genes.The functions of Na+ transporters which are involved in the maintenance and reconstruction of the ion homeostasis,transcriptional regulators and osmotic regulation genes were reviewed.Salt tolerance of plants are enhanced by Na+ vacuolar compartmentation or efflux or high levels of osmoprotectants accumulation in cytoplasm. 展开更多
关键词 RICE Salt stress Transport protein Transcription factor Osmotic adjustment
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Fluorescence Enhancement of Polyamine Derivatives of 1,8-Naphthalimide with Transition Metal Ions
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作者 文国涛 朱满洲 +3 位作者 王卓 孟祥明 胡惠媛 郭庆祥 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 北大核心 2006年第6期506-510,共5页
A series of fluorescent chemosensors 1-3 were synthesized to detect transition metal ions. At the room temperature, fluorescence intensities of these chemosensors in acetonitrile without transition metal ions were fou... A series of fluorescent chemosensors 1-3 were synthesized to detect transition metal ions. At the room temperature, fluorescence intensities of these chemosensors in acetonitrile without transition metal ions were found to be very weak, due to the process of the efficient intramolecular photoinduced electron transfer (PET). However, after addition of the transition metal ions, the chemoscnsor 1-3 exhibits obvious fluorescence enhancement. Moreover, the intensity of the fluorescence emission of chemosensors increases significantly in the presence of Zn^2+ and Cd^2+. The fluorescent chemosensors with different polyamine as receptors show diverse affinity abilities to the transition metal ions and signal the receptor-metal ion interaction by the intensity change of fluorescence emission. 展开更多
关键词 Transition metal ion 1 8-naphthalimide Photoinduced electron transfer Fluorescence enhancement Fluorescent chemosensor
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One-Step Realization of SWAP Gate with Superconducting Quantum-Interference Devices and Atoms in Cavity QED 被引量:1
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作者 ZHAN Zhi-Ming 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第6期1603-1606,共4页
We put forward a simple scheme for one-step realization of a two-qubit SWAP gate with SQUIDs (superconducting quantum-interference devices) in cavity QED via Raman transition. In this scheme, the cavity field is onl... We put forward a simple scheme for one-step realization of a two-qubit SWAP gate with SQUIDs (superconducting quantum-interference devices) in cavity QED via Raman transition. In this scheme, the cavity field is only virtually excited and thus the cavity decay is suppressed. The SWAP gate is realized by using only two lower fiux states of the SQUID system and the excited state would not be excited. Therefore, the effect of decoherence caused from the levels of the SQUID system is possibly minimized. The scheme can also be used to implement the SWAP gate with atoms. 展开更多
关键词 SWAP gate SUPERCONDUCTING Raman transition
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