近年来,研究真核基因的转录调控已成为分子生物学的热点之一。为了研究 DNA 元件或蛋白质因子在转录过程中的作用,建立无细胞体外转录体系是十分必要的。到目前为止,发现至少有六种必需的蛋白质因子参与 RNA 聚合酶Ⅱ的起始转录。绝大部...近年来,研究真核基因的转录调控已成为分子生物学的热点之一。为了研究 DNA 元件或蛋白质因子在转录过程中的作用,建立无细胞体外转录体系是十分必要的。到目前为止,发现至少有六种必需的蛋白质因子参与 RNA 聚合酶Ⅱ的起始转录。绝大部分RNA 聚合酶Ⅱ的无细胞转录体系来自哺乳动物或果蝇的组织培养细胞的提取物。本文研究了人的白介素-2受体α链基因(IL-2Rα)在 HeLa 细胞提取物中的转录。展开更多
The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In...The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited the vpu gene expression; the other one over-expressed the tetherin. Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses. The retroviral vector plasmids＇ functions of tetherin over-expression or vpu-RNAi were detected at the cell level. Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3TOV, and then mixed retroviruses were harvested. The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bi cells. The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.展开更多
Recently, we have analyzed expressional transcriptome and proteome for a number of cancer cell lines, including 8 hepatocellular carcinoma （HCC） ones [1,2]. These 8 HCC cell lines consist of 6 metastatic （all with ...Recently, we have analyzed expressional transcriptome and proteome for a number of cancer cell lines, including 8 hepatocellular carcinoma （HCC） ones [1,2]. These 8 HCC cell lines consist of 6 metastatic （all with mutant p53） and 2 non-metastatic （with a mutant and a lost p53） ones, being all HBV traceable for relatively close genetic-backgrounds. We have analyzed the related dataset and obtained transcrip- tome （16353 genes） and proteome （7861 proteins） of these 8 HCC cell lines, and explored a group of up-regulated and down-regulated genes [3].展开更多
文摘近年来,研究真核基因的转录调控已成为分子生物学的热点之一。为了研究 DNA 元件或蛋白质因子在转录过程中的作用,建立无细胞体外转录体系是十分必要的。到目前为止,发现至少有六种必需的蛋白质因子参与 RNA 聚合酶Ⅱ的起始转录。绝大部分RNA 聚合酶Ⅱ的无细胞转录体系来自哺乳动物或果蝇的组织培养细胞的提取物。本文研究了人的白介素-2受体α链基因(IL-2Rα)在 HeLa 细胞提取物中的转录。
基金National Natural Science Foundation of China(81101245,30970162)The Fundamental Research Funds for the Central Universities(65011871)National Training Programs of Innovation for Undergraduates(111005505)
文摘The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited the vpu gene expression; the other one over-expressed the tetherin. Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses. The retroviral vector plasmids＇ functions of tetherin over-expression or vpu-RNAi were detected at the cell level. Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3TOV, and then mixed retroviruses were harvested. The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bi cells. The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.
基金the International S&T Cooperation Program of China (2014DFB30020)the National Basic Research Program of China (2013CB910502, 2011CB910600)+1 种基金the National High Technology Research and Development Program of China(2012AA020200)the National Science and Technology Major Project (2012ZX1002012)
文摘Recently, we have analyzed expressional transcriptome and proteome for a number of cancer cell lines, including 8 hepatocellular carcinoma （HCC） ones [1,2]. These 8 HCC cell lines consist of 6 metastatic （all with mutant p53） and 2 non-metastatic （with a mutant and a lost p53） ones, being all HBV traceable for relatively close genetic-backgrounds. We have analyzed the related dataset and obtained transcrip- tome （16353 genes） and proteome （7861 proteins） of these 8 HCC cell lines, and explored a group of up-regulated and down-regulated genes [3].
