营养条件对转生长激素基因鲤生长和性腺发育的影响

运用形态学和组织学方法对转生长激素基因鲤在不同营养条件下的生长、饲料占有情况和性腺发育进程进行了研究。结果表明:正常投饲营养充分的池塘中转基因鲤与对照鲤的体重增长率分别是99.6%和68.2%;而在半饥饿饲料短缺池塘中的转基因鲤和对照鲤体重增长率分别为51.3%和49.8%。说明正常投喂饲料条件下转基因鲤的生长速度明显快于对照鲤;半饥饿饲料短缺条件下转基因鲤的体重增长速度与对照鲤无显著性差异。在7、8、9月份,对不同时期性腺发育状况检验结果表明,饲养在不同营养条件下的两种鱼的雌、雄性腺发育速度不同,但差异均不显著(p>0.05)。

转大麻哈鱼生长激素基因鲤食用安全毒性分析

以转基因鲤和非转基因鲤鱼肉(受试物)作为大鼠的饲料基础蛋白成分,从毒性方面开展转基因鲤的食用安全检测分析。急性经口毒性检测结果表明,实验期间,受试动物未见明显的中毒表现,无死亡,尸检中主要脏器亦未见异常,说明转大麻哈鱼生长激素基因鲤食用无毒。将转基因鲤以不同质量分数(10%、5%、2.5%)掺入饲料喂饲大鼠90d,检测结果表明,受试动物活动自如,被毛有光泽,鼻、眼、口腔无异常分泌物。一般毒性检测指标与阴性对照组比较,无显著性差异;末见该受试物对大鼠的血液学指标、血生化指标、尿常规和生化指标、脏器系数、病理组织学指标有不良影响。据此评价转大麻哈鱼生长激素基因鲤在大鼠实验阶段属无毒。

The integration characteristics of the exogenous growth hormone gene in a transgenic common carp(Cyprinus carpio L.) with fast-growth performance

The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an ‘‘all-fish'' growth hormone(GH)transgenic common carp family that exhibits fast growth.In this present study, genome walking, real-time quantitative polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an ATrich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.