本文以α-葡萄糖转苷酶为研究对象,研究了啤酒酵母自溶物替代商业酵母膏作为培养基对发酵产酶的影响,通过单因素试验得到最适的酵母自溶物的添加量为120 m L/L。在此基础上,以发酵酶活力为指标,通过正交试验法对黑曲霉发酵产酶的条件进...本文以α-葡萄糖转苷酶为研究对象,研究了啤酒酵母自溶物替代商业酵母膏作为培养基对发酵产酶的影响,通过单因素试验得到最适的酵母自溶物的添加量为120 m L/L。在此基础上,以发酵酶活力为指标,通过正交试验法对黑曲霉发酵产酶的条件进行了优化,结果表明,产酶最佳条件为发酵温度30℃,发酵初始p H 6.5,发酵时间120 h,摇床转速200 r/min,通过验证试验,得出α-葡萄糖转苷酶的酶活力可达200 U/m L。展开更多
The mechanisms for fine-tuning ABA level related to grape berry ripening remain elusive. Here, transcription analysis showed that the mRNA expression level of 9-cis-epoxycarotenoid dioxygenase gene （VvNCED1） increas...The mechanisms for fine-tuning ABA level related to grape berry ripening remain elusive. Here, transcription analysis showed that the mRNA expression level of 9-cis-epoxycarotenoid dioxygenase gene （VvNCED1） increases first, rapidly in mesocarp before the onset of grape berry ripening. After VvNCED1 peaks its expression level, ABA content increases rapidly in mesocarp coupled with an increase in both soluble sugar content and pH value. On the onset of berry ripening, VvNCED1 transcripts decline rapidly to its lowest point, then increases slightly. Whereas, the mRNA expression level of B-glucosidase gene VvBGI, on the whole, increases constantly during grape berry ripening. During berry de-greening, ABA glucosyltransferase （VvGT） and ABA 8＇-hydroxylase （VvCYPI） equilibrate ABA level; during berry coloring-up, VvGT predominantly equilibrates ABA level, namely, the up-regulation of ABA level mainly leads from VvNCED1 and VvBG1 gene high expression; the down-regulation of ABA level leads mainly from VvCYP! transcript level both in ABA content- and developmental phase-dependence manner. In conclusion, our main results show that VvNCED1 and VvBG1 genes are closely related to grape berry ripening.展开更多
OBJECTIVE： To investigate the effect of Qiguiyish- en decoction （QGYS） on the severity of nephropa- thy. METHODS： Twenty-four rats were randomly divid- ed into four groups （ Ⅰ,Ⅱ,Ⅲ, IV） according to the random...OBJECTIVE： To investigate the effect of Qiguiyish- en decoction （QGYS） on the severity of nephropa- thy. METHODS： Twenty-four rats were randomly divid- ed into four groups （ Ⅰ,Ⅱ,Ⅲ, IV） according to the random number table. Group I as control group did not establish nephropathy model. Groups Ⅱ, Ⅲ, and IV were intravenously administered Adria- mycin （7.5 mg/kg） through the tail vein to establish nephropathy model. QGYS was prepared with the extracts of Huangqi （RadixAstragali Mongolici）, Dan- ggui （Radix Angelicae Sinensis）, Niuxi （Radix Achyran-this Bidentatae）, and Chuanxiong （Rhizoma Chuanx- iong）. Group IV was administered QGYS （2 mL. kg^-1· d^-1）, groupⅢ was administered benazepril （10 mL. kg^-1·d^-1）, and group ⅠⅡ was administered water （2 mL. kg·^-1. d^-1） once daily for eight weeks. RESULTS： QGYS reduced the excretion of urinary protein and N-acetyH3-D-glucosaminidase and alle- viated the accumulation of extracellular matrix （ECM） in renal tissue. Additionally, QGYS effectively regulated the levels of transforming growth factor, tissue inhibitor of matrix metalloproteinase, and matrix metalloproteinases in the kidney of the rats. CONCLUSION-＂ QGYS may reduce the accumulation of ECM in the kidneys of rats with Adriamycin-in- duced nephropathy.展开更多
文摘本文以α-葡萄糖转苷酶为研究对象,研究了啤酒酵母自溶物替代商业酵母膏作为培养基对发酵产酶的影响,通过单因素试验得到最适的酵母自溶物的添加量为120 m L/L。在此基础上,以发酵酶活力为指标,通过正交试验法对黑曲霉发酵产酶的条件进行了优化,结果表明,产酶最佳条件为发酵温度30℃,发酵初始p H 6.5,发酵时间120 h,摇床转速200 r/min,通过验证试验,得出α-葡萄糖转苷酶的酶活力可达200 U/m L。
基金This study is supported by China National Nattu-al Science Foundation （Project 31040006） and Beijing Natural Science Foundation and Scientific Research Key Program of Beijing Commission of Education （NO. KZ200910020001）.
文摘The mechanisms for fine-tuning ABA level related to grape berry ripening remain elusive. Here, transcription analysis showed that the mRNA expression level of 9-cis-epoxycarotenoid dioxygenase gene （VvNCED1） increases first, rapidly in mesocarp before the onset of grape berry ripening. After VvNCED1 peaks its expression level, ABA content increases rapidly in mesocarp coupled with an increase in both soluble sugar content and pH value. On the onset of berry ripening, VvNCED1 transcripts decline rapidly to its lowest point, then increases slightly. Whereas, the mRNA expression level of B-glucosidase gene VvBGI, on the whole, increases constantly during grape berry ripening. During berry de-greening, ABA glucosyltransferase （VvGT） and ABA 8＇-hydroxylase （VvCYPI） equilibrate ABA level; during berry coloring-up, VvGT predominantly equilibrates ABA level, namely, the up-regulation of ABA level mainly leads from VvNCED1 and VvBG1 gene high expression; the down-regulation of ABA level leads mainly from VvCYP! transcript level both in ABA content- and developmental phase-dependence manner. In conclusion, our main results show that VvNCED1 and VvBG1 genes are closely related to grape berry ripening.
基金Supported by the Natural Science Foundation of China,Through Cell Signaling Pathway to Treat Chronic Kidney Disease by Chinese Herbs Modified Jiaweidangguibuxue Decottion(No.81273723)Jiangsu Administration of Traditional Chinese Medicine(No.LZ13235)the Natural Science Foundation of Bureau of Science and Technology of Suzhou(No.SYS201016)
文摘OBJECTIVE： To investigate the effect of Qiguiyish- en decoction （QGYS） on the severity of nephropa- thy. METHODS： Twenty-four rats were randomly divid- ed into four groups （ Ⅰ,Ⅱ,Ⅲ, IV） according to the random number table. Group I as control group did not establish nephropathy model. Groups Ⅱ, Ⅲ, and IV were intravenously administered Adria- mycin （7.5 mg/kg） through the tail vein to establish nephropathy model. QGYS was prepared with the extracts of Huangqi （RadixAstragali Mongolici）, Dan- ggui （Radix Angelicae Sinensis）, Niuxi （Radix Achyran-this Bidentatae）, and Chuanxiong （Rhizoma Chuanx- iong）. Group IV was administered QGYS （2 mL. kg^-1· d^-1）, groupⅢ was administered benazepril （10 mL. kg^-1·d^-1）, and group ⅠⅡ was administered water （2 mL. kg·^-1. d^-1） once daily for eight weeks. RESULTS： QGYS reduced the excretion of urinary protein and N-acetyH3-D-glucosaminidase and alle- viated the accumulation of extracellular matrix （ECM） in renal tissue. Additionally, QGYS effectively regulated the levels of transforming growth factor, tissue inhibitor of matrix metalloproteinase, and matrix metalloproteinases in the kidney of the rats. CONCLUSION-＂ QGYS may reduce the accumulation of ECM in the kidneys of rats with Adriamycin-in- duced nephropathy.
