Maize chlorotic dwarf virus (MCDV) is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification (R...Maize chlorotic dwarf virus (MCDV) is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was estab- lished in this study. Based on the sequence of MCDV coat protein coding gene, specific primers were designed and similar sensitivities were observed between RT- LAMP and RT-PCR, except that RT-LAMP was quicker, and the reaction could be finished within 1 h. In addition, the presence or absence of the fluorescent display in daylight allows naked easy detection of the amplification of MCDV genomic RNA using calcein. The RT-LAMP assay was applied successfully to detect MCDV in maize seeds, and the result by the addition of calcein was consistent with the result detected by the real time turbidimeter.展开更多
The design and development of a timer based revolution per minute(RPM)measurement system were described in this paper.The rotating shaft of a dc motor was used to measure the RPM and timer integrated circuit(IC)555 wa...The design and development of a timer based revolution per minute(RPM)measurement system were described in this paper.The rotating shaft of a dc motor was used to measure the RPM and timer integrated circuit(IC)555 was used in astable mode.The frequency of timer output waveform measured by a digital storage oscilloscope(DSO)is almost linearly proportional to the RPM of rotating shaft,and the RPM also linearly varies with the change of the external input voltage level.Hence the linear relationship between the frequency of timer output waveform and the RPM can be obtained.The main advantages of this developed system are linear input-output relationship,small size,easy to carry and cost effective.展开更多
The instantaneous speed of a hydraulic system contains a wealth of operational information,and its accurate extraction is the basis for condition monitoring and fault diagnosis.In order to solve the problem of high ha...The instantaneous speed of a hydraulic system contains a wealth of operational information,and its accurate extraction is the basis for condition monitoring and fault diagnosis.In order to solve the problem of high hardware requirement for instantaneous speed measurement based on data acquisition card,a new method of high precision measurement is proposed.In this method,the time-displacement information of each tooth is obtained from the pulsed square wave signal of the gear disk collected by magnetoelectric sensors.The time-displacement curve is interpolated by the cubic spline interpolation method,and then the instantaneous speed is calculated by the five-point digital differential formula.The experimental results show that the method improves the speed measurement resolution and reduces the quantization error.The high precision instantaneous speed signal can also be acquired by hardware devices with less teeth and low sampling frequency.The related research results provide a theoretical basis and a method for improving the accuracy of instantaneous speed measurement.展开更多
For the test of rotation speed of high spinning projectile, the general formula of the motional electromotive force (MEMF) of planar magnetic induction coil (PMIC) is derived in case of 3D rotation in a stable mag...For the test of rotation speed of high spinning projectile, the general formula of the motional electromotive force (MEMF) of planar magnetic induction coil (PMIC) is derived in case of 3D rotation in a stable magnetic field. Under a reasona-ble assumption, the MEMF of PMIC is simplified after the aforementioned general formula is used to calculate high spinning PMIC in the geomagnetic field environment. The determination approach of half-cycle is discussed and the method of rotation speed test is studied, and a test is conducted in the paper. The rotation speed curve obtained by the approach in this paper is consistent with the curve by telemetry.展开更多
In order to study the transmission efficiency of engine and optimize the structure of driving wheel,the rotational speed storage test device of driving wheel in tracked vehicle based on magnetoelectric sensor was desi...In order to study the transmission efficiency of engine and optimize the structure of driving wheel,the rotational speed storage test device of driving wheel in tracked vehicle based on magnetoelectric sensor was designed.The device consisted of a mounting bracket,a sensor and a tester.The mounting bracket was installed in vehicle body after fixing the tester and sensor to mounting bracket beside the driving wheel.Using the storage test instrument,the wireless trigger technology was applied to synchronously record and stored the rotational speed data of the driving wheel in tracked vehicle.After the experiment was finished,the data was read out through the upper computer.Both valid data and satisfactory results were obtained through both simulated and actual vehicle tests.展开更多
This paper presents the composition, control scheme and signal processing method of frequency conversion motor speed testing system based on $7-200 PLC, and design the hardware circuit, the trapezoidal chart procedure...This paper presents the composition, control scheme and signal processing method of frequency conversion motor speed testing system based on $7-200 PLC, and design the hardware circuit, the trapezoidal chart procedure and touch screen display program. The debugging test shows that the system is simple, practical, and related design work has laid the foundation for the future.展开更多
Rotor time constant is an important parameter for the indirect lleld oraentateO control of mauc- tion motor. Incorrect rotor tittle constant value will cause the flux observer generating a wrong angu- lar orientation ...Rotor time constant is an important parameter for the indirect lleld oraentateO control of mauc- tion motor. Incorrect rotor tittle constant value will cause the flux observer generating a wrong angu- lar orientation of the rotor field. A new approach serves for rotor time constant on-line adaptation by setting the stator current to be zero for a short period. A smooth eorrector is designed to prevent ab- normal detection result from making adaptation. Impact of zero current duration on detection error and rotor speed is analyzed by experiments.展开更多
Ordinary columns of jet grouting are circular or fan-shaped. But in recent years, such shapes as candy-shaped columns were put to practical use. They are constructed by switching the rotational speed of the stem in on...Ordinary columns of jet grouting are circular or fan-shaped. But in recent years, such shapes as candy-shaped columns were put to practical use. They are constructed by switching the rotational speed of the stem in one rotation. We applied this method to swing jet grouting. That is, we changed the rotational speeds in the swing jet grouting to make elongated columns near rectangle. The test construction was succeeded and the columns’ shapes conformed to those designed. In addition, the quality was confirmed to be as good as ordinary columns. Radii (distances from center to outer ends) were 1.03 to 1.21 times as large as those designed. Improvement ratios were 95% or more in any position. Strengths were varied by location, but they satisfied design strength 1.5 kN/m2 at any position. We concluded that this new column can be applied to practice in the same way as ordinary columns.展开更多
To increase the machine accuracy by improving the stiffness of bearings,a preload was applied to bearings.A variable preload technology was necessary to perform machining processes in both low and high speed regions.A...To increase the machine accuracy by improving the stiffness of bearings,a preload was applied to bearings.A variable preload technology was necessary to perform machining processes in both low and high speed regions.An automatic variable preload device was fabricated using an eccentric mass.By installing the fabricated device on a spindle,the effect of the automatic variable preload device on the performance of the spindle was analyzed.In the results of the vibration measurement of the spindle,the vibration is increased by 20%-37% according to measurement points at the maximum rotation speed of 5 000 r/min.And,in the results of the noise measurement of the spindle,the spindle rotation speed is increased by about 1.9% and 1.5% at the front and side of the spindle,respectively.Based on the results of this analysis,an improved method that reduces such effects on the performance of the spindle is proposed.展开更多
Effects of ball-milling parameter on structures and properties of sintered Mg-l.5Zr (mass fraction, %) alloy were researched by metallographic analysis, mechanical properties tests and DMA technology. The results in...Effects of ball-milling parameter on structures and properties of sintered Mg-l.5Zr (mass fraction, %) alloy were researched by metallographic analysis, mechanical properties tests and DMA technology. The results indicate that with 310 r/min rotation speed, the microstructure of the sintered alloy is greatly refined, and Zr-phase distributes uniformly. The micro-hardness, bending strength and damping capacities are the greatest under 310 r/min rotation speed. The damping peak of sintered Mg-l.5Zr alloy increases with increasing frequency under the testing conditions. The relaxation time meets the Arrhenius relationship, and shows the characteristics of relaxation damping.展开更多
In order to meet the technical requirements of grinding the circumferential cutting edge of indexable inserts, thermo-mechanical properties of bowl-shaped grinding wheel in high speed grinding process and the influenc...In order to meet the technical requirements of grinding the circumferential cutting edge of indexable inserts, thermo-mechanical properties of bowl-shaped grinding wheel in high speed grinding process and the influence of dimension variations of the grinding wheel on machining accuracy were investigated. Firstly, the variation trends of the dimension due to centrifugal force generated in different wheel speeds were studied and the effect of stress stiffening and spin softening was presented. Triangular heat flux distribution model was adopted to determine temperature distribution in grinding process. Temperature field cloud pictures were obtained by the finite element software. Then, dimension variation trends of wheel structure were acquired by considering the thermo-mechanical characteristic under combined action of centrifugal force and grinding heat at different speeds. A method of online dynamic monitoring and automatic compensation for dimension error of indexable insert was proposed. By experimental verification, the precision of the inserts satisfies the requirement of processing.展开更多
We study the effects of electrolyte temperature on DNA molecule translocation experimentally without and with a temperature gradient across nanopore membranes.The same temperatures on both electrolyte chambers are fir...We study the effects of electrolyte temperature on DNA molecule translocation experimentally without and with a temperature gradient across nanopore membranes.The same temperatures on both electrolyte chambers are first considered.The DNA molecule translocation time is measured to be 2.44 ms at 2°C in both chambers,which is 1.57 times longer than at 20°C.Then the temperature difference effect is characterized in both chambers.The results show that the DNA translocation speed can be slowed down as long as one side temperature is lowered,irrespective of the temperature gradient direction.This indicates that the thermophoretic driving force generated by a temperature gradient has no obvious effect on the threading speed of DNA molecules,while the main reason for the slowed DNA translocation speed is the increased viscosity.Interestingly,the capture rate of DNA molecules is enhanced under a temperature gradient condition,and the capture rate during DNA translocation from hot side at 21°C to cold one at 2°C is 1.7 times larger than that under the condition of both chambers at 20°C.Finally,an optimized configuration is proposed to acquire higher capture rates and lower DNA translocation speeds.展开更多
Brush scrubber cleaning is widely used for post chemical mechanical polishing(CMP)cleaning in semiconductor manufacturing.In this study,an experimental system based on fluorescence technique and particle-tracking velo...Brush scrubber cleaning is widely used for post chemical mechanical polishing(CMP)cleaning in semiconductor manufacturing.In this study,an experimental system based on fluorescence technique and particle-tracking velocimetry(PTV)technique was employed to characterize the particle removal displacement and velocity in the interface between a transparent copper film and a porous polyvinyl alcohol(PVA)brush during the cleaning process.Several different cleaning conditions including rotation speeds,loading pressure and cleaning agent were examined and the particle removal rate was compared.Elastic and friction removal,hydrodynamic removal and mixed-type removal are the three types of particle removal.Particles with an arc trace and uniform velocity curves were removed by friction and elastic force which were related to the brush load.Particles with a random trace and fluctuant velocity curves were removed by hydrodynamic force which was determined by the brush rotation speed.The increase of particle removal rate(PRR)with brush rotation speed is a logistic function.It is easier to improve PRR by increasing the brush load or by adding surfactant than by increasing the brush rotation speed.展开更多
The entry-glide guidance strategy for hypersonic vehicles that can satisfy both terminal and path constraints is investigated in this paper.We propose a quasi-equilibrium glide adaptive guidance methodology based on t...The entry-glide guidance strategy for hypersonic vehicles that can satisfy both terminal and path constraints is investigated in this paper.We propose a quasi-equilibrium glide adaptive guidance methodology based on the quasi-equilibrium glide condition(QEGC),which innovatively utilizes the quasi-equilibrium glide phenomenon in lifting entry.With the aid of QEGC,both range and terminal velocity can be predicted analytically with high precision.The path constraints are converted into angle of attack constraints,which has been difficult to realize by using traditional predictive guidance methods.The algorithm is independent of the standard trajectory.All the guidance commands,including the bank angle and the angle of attack,are calculated analytically in real time,which endows the algorithm with sufficient adapbility.The results of a CAV-H vehicle guidance test show that the algorithm leads the vehicle along a quasi-equilibrium glide trajectory satisfying both the terminal and path constraints and has sufficient flexibility for occasional mission changes.Furthermore,the robustness of the guidance algorithm under disturbances is validated through a Monte Carlo simulation.展开更多
The changes of numeraire can be used as a very powerful tool in pricing contingent claims in the context of a complete market.By using the method of numeraire changes to evaluate convertible bonds when the value of fi...The changes of numeraire can be used as a very powerful tool in pricing contingent claims in the context of a complete market.By using the method of numeraire changes to evaluate convertible bonds when the value of firm,and those of zero-coupon bonds follow general adapted stochastic processes in this paper,using Ito theorem and Gisanov theorem.A closed-form solution is derived under the stochastic volatility by using fast Fourier transforms.展开更多
文摘Maize chlorotic dwarf virus (MCDV) is a quarantine pest as approved by Chinese government. A rapid, sensitive and specific MCDV detection method using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was estab- lished in this study. Based on the sequence of MCDV coat protein coding gene, specific primers were designed and similar sensitivities were observed between RT- LAMP and RT-PCR, except that RT-LAMP was quicker, and the reaction could be finished within 1 h. In addition, the presence or absence of the fluorescent display in daylight allows naked easy detection of the amplification of MCDV genomic RNA using calcein. The RT-LAMP assay was applied successfully to detect MCDV in maize seeds, and the result by the addition of calcein was consistent with the result detected by the real time turbidimeter.
文摘The design and development of a timer based revolution per minute(RPM)measurement system were described in this paper.The rotating shaft of a dc motor was used to measure the RPM and timer integrated circuit(IC)555 was used in astable mode.The frequency of timer output waveform measured by a digital storage oscilloscope(DSO)is almost linearly proportional to the RPM of rotating shaft,and the RPM also linearly varies with the change of the external input voltage level.Hence the linear relationship between the frequency of timer output waveform and the RPM can be obtained.The main advantages of this developed system are linear input-output relationship,small size,easy to carry and cost effective.
基金National Natural Science Foundation of China(No.51275375)Scientific Research Program Project of Shaanxi Provincial Education Department(No.19JK0464)
文摘The instantaneous speed of a hydraulic system contains a wealth of operational information,and its accurate extraction is the basis for condition monitoring and fault diagnosis.In order to solve the problem of high hardware requirement for instantaneous speed measurement based on data acquisition card,a new method of high precision measurement is proposed.In this method,the time-displacement information of each tooth is obtained from the pulsed square wave signal of the gear disk collected by magnetoelectric sensors.The time-displacement curve is interpolated by the cubic spline interpolation method,and then the instantaneous speed is calculated by the five-point digital differential formula.The experimental results show that the method improves the speed measurement resolution and reduces the quantization error.The high precision instantaneous speed signal can also be acquired by hardware devices with less teeth and low sampling frequency.The related research results provide a theoretical basis and a method for improving the accuracy of instantaneous speed measurement.
基金National Key Lab for Electronic Measurement and Technology,North University of China(No.9140C120401080C12)
文摘For the test of rotation speed of high spinning projectile, the general formula of the motional electromotive force (MEMF) of planar magnetic induction coil (PMIC) is derived in case of 3D rotation in a stable magnetic field. Under a reasona-ble assumption, the MEMF of PMIC is simplified after the aforementioned general formula is used to calculate high spinning PMIC in the geomagnetic field environment. The determination approach of half-cycle is discussed and the method of rotation speed test is studied, and a test is conducted in the paper. The rotation speed curve obtained by the approach in this paper is consistent with the curve by telemetry.
基金Cultivation Programs for Young Scientific Research Personnel of Higher Education Institutions in Shanxi Province(No.2019QPJH18)Fund for“1331 Project”Key Innovative Research Team of Shanxi Province(No.1331KIPT)。
文摘In order to study the transmission efficiency of engine and optimize the structure of driving wheel,the rotational speed storage test device of driving wheel in tracked vehicle based on magnetoelectric sensor was designed.The device consisted of a mounting bracket,a sensor and a tester.The mounting bracket was installed in vehicle body after fixing the tester and sensor to mounting bracket beside the driving wheel.Using the storage test instrument,the wireless trigger technology was applied to synchronously record and stored the rotational speed data of the driving wheel in tracked vehicle.After the experiment was finished,the data was read out through the upper computer.Both valid data and satisfactory results were obtained through both simulated and actual vehicle tests.
文摘This paper presents the composition, control scheme and signal processing method of frequency conversion motor speed testing system based on $7-200 PLC, and design the hardware circuit, the trapezoidal chart procedure and touch screen display program. The debugging test shows that the system is simple, practical, and related design work has laid the foundation for the future.
基金Supported by the National Natural Science Foundation of China(No.51276016)the Fundamental Research Funds for the Central University(No.FRF-TP-12-059A)
文摘Rotor time constant is an important parameter for the indirect lleld oraentateO control of mauc- tion motor. Incorrect rotor tittle constant value will cause the flux observer generating a wrong angu- lar orientation of the rotor field. A new approach serves for rotor time constant on-line adaptation by setting the stator current to be zero for a short period. A smooth eorrector is designed to prevent ab- normal detection result from making adaptation. Impact of zero current duration on detection error and rotor speed is analyzed by experiments.
文摘Ordinary columns of jet grouting are circular or fan-shaped. But in recent years, such shapes as candy-shaped columns were put to practical use. They are constructed by switching the rotational speed of the stem in one rotation. We applied this method to swing jet grouting. That is, we changed the rotational speeds in the swing jet grouting to make elongated columns near rectangle. The test construction was succeeded and the columns’ shapes conformed to those designed. In addition, the quality was confirmed to be as good as ordinary columns. Radii (distances from center to outer ends) were 1.03 to 1.21 times as large as those designed. Improvement ratios were 95% or more in any position. Strengths were varied by location, but they satisfied design strength 1.5 kN/m2 at any position. We concluded that this new column can be applied to practice in the same way as ordinary columns.
基金Project(2011-0027035) supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Education,Science and Technology,Korea
文摘To increase the machine accuracy by improving the stiffness of bearings,a preload was applied to bearings.A variable preload technology was necessary to perform machining processes in both low and high speed regions.An automatic variable preload device was fabricated using an eccentric mass.By installing the fabricated device on a spindle,the effect of the automatic variable preload device on the performance of the spindle was analyzed.In the results of the vibration measurement of the spindle,the vibration is increased by 20%-37% according to measurement points at the maximum rotation speed of 5 000 r/min.And,in the results of the noise measurement of the spindle,the spindle rotation speed is increased by about 1.9% and 1.5% at the front and side of the spindle,respectively.Based on the results of this analysis,an improved method that reduces such effects on the performance of the spindle is proposed.
基金Project(20080430221) supported by China Postdoctoral Science Foundation Project(2008ZF52058) supported by the Aeronautical Science Foundation of ChinaProject(2009A610026) supported by the Natural Science Foundation of Ningbo City,China
文摘Effects of ball-milling parameter on structures and properties of sintered Mg-l.5Zr (mass fraction, %) alloy were researched by metallographic analysis, mechanical properties tests and DMA technology. The results indicate that with 310 r/min rotation speed, the microstructure of the sintered alloy is greatly refined, and Zr-phase distributes uniformly. The micro-hardness, bending strength and damping capacities are the greatest under 310 r/min rotation speed. The damping peak of sintered Mg-l.5Zr alloy increases with increasing frequency under the testing conditions. The relaxation time meets the Arrhenius relationship, and shows the characteristics of relaxation damping.
基金Project(2010ZX04001-162)supported by the National Science and Technology Major Project of China
文摘In order to meet the technical requirements of grinding the circumferential cutting edge of indexable inserts, thermo-mechanical properties of bowl-shaped grinding wheel in high speed grinding process and the influence of dimension variations of the grinding wheel on machining accuracy were investigated. Firstly, the variation trends of the dimension due to centrifugal force generated in different wheel speeds were studied and the effect of stress stiffening and spin softening was presented. Triangular heat flux distribution model was adopted to determine temperature distribution in grinding process. Temperature field cloud pictures were obtained by the finite element software. Then, dimension variation trends of wheel structure were acquired by considering the thermo-mechanical characteristic under combined action of centrifugal force and grinding heat at different speeds. A method of online dynamic monitoring and automatic compensation for dimension error of indexable insert was proposed. By experimental verification, the precision of the inserts satisfies the requirement of processing.
基金supported by the National Basic Research Program of China("973"Project)(Grant Nos.2011CB707601 and 2011CB707605)the National Natural Science Foundation of China(Grants Nos.50925519 and 51375092)+1 种基金supported by the Scientific Research Foundation of Graduate School of Southeast University(Grant No.YBJJ1004)supported by the Fundamental Research Funds for the Central Universities and the Innovative Project for Graduate Students of Jiangsu Province(Grant No.KYLX_0100)
文摘We study the effects of electrolyte temperature on DNA molecule translocation experimentally without and with a temperature gradient across nanopore membranes.The same temperatures on both electrolyte chambers are first considered.The DNA molecule translocation time is measured to be 2.44 ms at 2°C in both chambers,which is 1.57 times longer than at 20°C.Then the temperature difference effect is characterized in both chambers.The results show that the DNA translocation speed can be slowed down as long as one side temperature is lowered,irrespective of the temperature gradient direction.This indicates that the thermophoretic driving force generated by a temperature gradient has no obvious effect on the threading speed of DNA molecules,while the main reason for the slowed DNA translocation speed is the increased viscosity.Interestingly,the capture rate of DNA molecules is enhanced under a temperature gradient condition,and the capture rate during DNA translocation from hot side at 21°C to cold one at 2°C is 1.7 times larger than that under the condition of both chambers at 20°C.Finally,an optimized configuration is proposed to acquire higher capture rates and lower DNA translocation speeds.
基金supported by the National Natural Science Foundation of China(Grant No.51205006)the Tribology Science Fund of State Key Laboratory of Tribology and the Program for Excellent Talents by the Beijing Ministry of Organization
文摘Brush scrubber cleaning is widely used for post chemical mechanical polishing(CMP)cleaning in semiconductor manufacturing.In this study,an experimental system based on fluorescence technique and particle-tracking velocimetry(PTV)technique was employed to characterize the particle removal displacement and velocity in the interface between a transparent copper film and a porous polyvinyl alcohol(PVA)brush during the cleaning process.Several different cleaning conditions including rotation speeds,loading pressure and cleaning agent were examined and the particle removal rate was compared.Elastic and friction removal,hydrodynamic removal and mixed-type removal are the three types of particle removal.Particles with an arc trace and uniform velocity curves were removed by friction and elastic force which were related to the brush load.Particles with a random trace and fluctuant velocity curves were removed by hydrodynamic force which was determined by the brush rotation speed.The increase of particle removal rate(PRR)with brush rotation speed is a logistic function.It is easier to improve PRR by increasing the brush load or by adding surfactant than by increasing the brush rotation speed.
基金supported by the National Natural Science Foundation of China (Grant No. 61104200)
文摘The entry-glide guidance strategy for hypersonic vehicles that can satisfy both terminal and path constraints is investigated in this paper.We propose a quasi-equilibrium glide adaptive guidance methodology based on the quasi-equilibrium glide condition(QEGC),which innovatively utilizes the quasi-equilibrium glide phenomenon in lifting entry.With the aid of QEGC,both range and terminal velocity can be predicted analytically with high precision.The path constraints are converted into angle of attack constraints,which has been difficult to realize by using traditional predictive guidance methods.The algorithm is independent of the standard trajectory.All the guidance commands,including the bank angle and the angle of attack,are calculated analytically in real time,which endows the algorithm with sufficient adapbility.The results of a CAV-H vehicle guidance test show that the algorithm leads the vehicle along a quasi-equilibrium glide trajectory satisfying both the terminal and path constraints and has sufficient flexibility for occasional mission changes.Furthermore,the robustness of the guidance algorithm under disturbances is validated through a Monte Carlo simulation.
基金supported by the National key scientific instrument and Equipment Development Program of China under Grant No.2012YQ220119Anhui Provincial Natural Science Foundation under Grant No.1308085 MF93+1 种基金the Fundamental Research Foundation for the Central Universities under Grant No.2013HGXJ0223National Natural Science Foundations of China under Grant No.11201108
文摘The changes of numeraire can be used as a very powerful tool in pricing contingent claims in the context of a complete market.By using the method of numeraire changes to evaluate convertible bonds when the value of firm,and those of zero-coupon bonds follow general adapted stochastic processes in this paper,using Ito theorem and Gisanov theorem.A closed-form solution is derived under the stochastic volatility by using fast Fourier transforms.