B7-1基因转染对小鼠前胃癌细胞增殖活性的影响

目的 观察B7- 1基因转染对小鼠前胃癌细胞 (MFC)增殖活性的影响。方法 通过脂质体介导将B7- 1基因转染MFC细胞 ,G4 18筛选阳性克隆后 ,用MTT法和流式细胞仪 (FCM )分别测定转染然细胞增殖活性的变化 ,并以空载体 pcDNA3转染MFC细胞作为对照。 结果 转染B7- 1基因的MFC细胞增殖活性受到抑制。结论 转染B7- 1基因的MFC细胞增殖活性受到抑制 ,提示B7-

B7-1基因转染小鼠EL-4淋巴瘤细胞的致瘤性研究

目的研究Ｂ７１基因转染小鼠ＥＬ４淋巴瘤细胞的致瘤性，为制备基因转染瘤苗提供实验依据。方法将Ｂ７１基因转染小鼠ＥＬ４淋巴瘤细胞后，用Ｇ４１８选择和克隆的方法获得高表达Ｂ７１分子的ＥＬ４Ｂ７１＋细胞，然后进行小鼠的体内致瘤试验。结果ＥＬ４Ｂ７１＋细胞接种小鼠后，与野生型ＥＬ４（ＥＬ４Ｗｔ）相比，肿瘤结节形成时间和荷瘤小鼠存活时间明显延长，ＥＬ４Ｂ７１＋细胞协同ＩＬ２接种小鼠后，效果更加显著。结论Ｂ７１基因转染小鼠ＥＬ４淋巴瘤细胞的致瘤性比野生型ＥＬ４（ＥＬ４Ｗｔ）低。

转B_7基因在泌尿系肿瘤治疗中的应用前景

转Ｂ７基因在泌尿系肿瘤治疗中的应用前景邢念增俞莉章郭应禄作者单位：１０００３４北京医科大学第一医院，泌尿外科研究所人类的基因治疗是指将目的基因导入人体细胞，以达到治疗疾病的方法［１］。近十年随着ＤＮＡ重组技术的发展与对疾病分子生物学机制认识的深入，使...

B_7基因转染治疗原发性肝癌的实验研究

Ｂ７基因转染治疗原发性肝癌的实验研究袁爱力张智翔肖丽萍侯淑琴肿瘤细胞缺乏主要组织相容性复合体（ＭＨＣ）－Ⅰ类抗原表达是肿瘤患者免疫监视功能低下的重要原因。实验研究已证实将ＭＨＣ－Ⅰ类基因Ｈ－２Ｋ导入到鼠白血病细胞中后不仅可以表达，而且杀伤活性亦增强［...

Therapeutic antitumor response to cervical cancer in mice immunized with U14 vaccines transfected with costimulatory B7 gene

Objective To investigate the effect of U14 vaccine transfected with the B7 gene in inducing antitumor immune response to murine cervical carcinoma in Chinese 615-strain mice.Methods A recombinant retroviral plasmid vector expressing mouse B7-1 gene (pLNSX-mB7) was transfected into 615-strain mouse cervical carcinoma cell line No. 14 (U14) by electroporation to set up a highly-expressed mB7-1 U14 cell clonal strain (B7+U14). In vivo experiments: (1) B7+U14 vaccine was primed to protect the 615-strain mice against U14 re-challenge. (2) B7+U14 vaccine was injected into tumor-bearing mice with different tumor sizes. Lifetimes and tumor sizes were recorded. In vitro cytotoxicity assay: Mice were immunized with B7+U14 or U14 vaccine and 2 weeks later, spleen cells of those mice were cultured for 2 days. The cytotoxicity of these cells against U14 was detected by 5-diphenyl tetrazolium bromide assay.Results We obtained several B7-1 high expression clonal U14 lines. In vivo experiment, we did not find tumor growing in 3 of the 6 mice primed by B7+U14 vaccine during their entire life after re-challenge with U14. The other 3 mice developed tumors and their average survival time was longer than that of the control group (P<0.01). All 6 mice grew tumors in the control group. When the transplanted tumors became palpable, the mice were randomly divided into 3 groups to be injected with B7+U14 vaccine. It was effective for tumor-bearing mice only when the tumor diameters were <3?mm. When the diameters were ≥3?mm, it was not efficacious to inject B7+U14 vaccine (P<0.05). In vitro cytotoxicity assay, cytotoxic T lymphocytes induced by B7+U14 vaccine had a higher cytotoxicity against U14 than that induced by U14 vaccine (F=310.8, P<0.001).Conclusions Vaccines of cervical cancer cells transfected with the costimulatory molecule B7 gene can induce antitumor immune protection in host mice against U14 re-challenge. This treatment may cure part of the tumor-bearing mice but be restricted by tumor size. The results suggest that transfecting the B7 gene into cervical cancer as a cell vaccine may be an efficient supplementary method to treat cervical cancer after operation.

骨骼

survtvin在多发性骨髓瘤患者骨髓细胞中的表达及其意义；B7-1基因转染对骨肉瘤细胞生物学行为的影响；唑来膦酸治疗恶性肿瘤骨转移疼痛的临床研究；FND方案治疗难治性、复发性骨髓瘤的初步报告；注射用唑来膦酸治疗恶性肿瘤溶骨性骨转移疼痛的Ⅱ期临床试验；应用反义TGF-β1基因进行骨肉瘤免疫基因治疗的研究。