添加磷对水稻-油菜轮作土壤N2O排放影响

我国南方红壤区域普遍属于缺磷土壤,种植作物需要施用较多的磷肥,但添加磷对水稻-油菜轮作土壤中N2O的排放影响并不明确.以潜江、咸宁两处水稻-油菜轮作模式下的土壤为研究对象,添加不同浓度的磷(0、15和30 mg·kg^-1)和不同浓度的氮(0和100 mg·kg^-1)进行室内培养实验,探究添加磷对水稻-油菜轮作土壤N2O排放的影响.结果表明,添加磷对土壤中N2O的排放有较为显著的影响,但影响的方式有所不同:在土壤本身氮比较少的情况下,添加磷会促进土壤中微生物对氮的固定,降低N2O的排放;在土壤中有充足的氮情况下,添加较少的磷会促进土壤中硝化微生物的活动,促进N2O的排放,但添加较多的磷同样会促进土壤中微生物对氮的固定,相比于添加较少的磷处理会抑制N2O的排放;在土壤本身磷的含量较为充足的情况下,无论土壤中的氮源是否充足,添加磷仅对土壤中N2O的排放起抑制作用.

推广土壤轮作控制蔬菜病害

合理轮作,不但能提高作物本身的抗逆能力,而且能使潜藏在地里的病原物经过一定的期限后大量减少或丧失侵染能力,达到使寄主作物轻微发病或不发病的目的。因此,合理轮作是蔬菜病害综合防治中的重要农防措施之一。随着农村经济体制的改革,以生产队为单位,成块成片地作物轮作已成为过去。菜农由于受地理条件、种植习惯和当地品种资源的限制,往往在有限的承包地上年年种植某几个蔬菜品种,无法进行合理轮作。由此而引起病原物逐年积累,寄主作物年年发病,杀菌剂越用越多,导致病害重、产量低、

花生不同连作年限应用微生物菌剂效果研究

在花生连作5年和轮作土壤上分别进行了3种微生物菌剂的应用试验,通过对花生主要生育期生长发育、叶片叶绿素含量和土壤微生物数量的观测,明确微生物菌剂的应用效果。结果表明,由黄孢平革菌、细黄链霉菌、多粘芽孢杆菌等组成的微生物菌剂3在连作土壤中效果最明显;轮作土壤中微生物菌剂1和2作用比较明显,其中以根瘤菌为主的菌剂1对产量的促进效果更显著。微生物菌剂对株高、茎叶鲜重、叶绿素含量以及产量等有促进作用,并且多个指标之间有较好的吻合性。

长期施用新鲜与腐熟畜禽粪肥土壤溶解性有机质与微生物群落的特征及其关联性

【目的】研究新鲜与腐熟鸡/猪粪长期施用下土壤溶解性有机质(DOM)的光学组分特征及其与微生物群落多样性和组成间的内在联系,为红壤区绿色循环农业实施策略的制定提供土壤生态方向的理论依据。【方法】基于国家土壤质量广州红壤观测试验站连续11年(2011—2022)的玉米-玉米-包菜轮作定位试验,包括5个处理,分别为不施肥对照、腐熟鸡粪、新鲜鸡粪、腐熟猪粪和新鲜猪粪。采集表层土壤样品,利用Illumina MiSeq高通量测序测定微生物群落,同时分析土壤DOM紫外-吸收特征及荧光吸收特征,测定相关化学性质,基于多元分析解析主要影响因子。【结果】腐熟粪肥显著提高了土壤有机质含量(122.5%—354.8%),腐熟鸡粪能有效提高土壤速效磷(1697.2%—3455.3%)和全磷(587.5%—812.5%)含量,腐熟猪粪主要提高土壤碱解氮(286.6%—311.3%)和全氮(326.4%—373.6%)含量。畜禽粪肥施用均显著提高了土壤DOM含量(60.3%—227.8%),其中猪粪处理对有色溶解性有机质(CDOM)含量提升效果最佳(118.1%—231.7%),鸡粪处理则增加土壤荧光溶解性有机质(FDOM)含量(293.4%—834.9%),且腐熟粪肥效果更佳(834.9%)。FDOM特征指标中,施肥处理的自生源指数均低于对照组(33.2%—39.2%),但腐殖化指数均高于对照组(40.3%—43.3%)。经平行因子分析拆分出4个荧光组分,施肥处理均主要富集C3(含富里酸和胡敏酸的中分子类腐殖质)和C4组分(含类色氨酸的大分子类腐殖质),促进FDOM类蛋白质成分转化为类腐殖质物质,且腐熟粪肥处理土壤C3与C4组分的最大荧光强度更高。腐熟鸡粪处理土壤具有更高微生物群落丰富度(Chao 1指数:19065.6)和多样性(Shannon指数:5.6—6.0),在提高微生物群落α多样性方面更具优势。不同处理对土壤微生物群落组成的影响存在差异,鸡粪处理以富营养类群Proteobacteria(31.2%—33.0%)、Gemmatimonadetes(4.1%)为主,猪粪处理以寡营养高效碳利用类群Acidobacteria(21.0%—21.6%)和硝化细菌类群Nitrospirae(2.6%—3.4%)为主。门与科水平共线性网络均以正关联为主,其中关联数最多的菌属为Rhodobacteraceae。冗余分析及随机森林模型预测均显示微生物群落主要受速效钾和DOM中C3组分影响,其中氮循环相关微生物类群的响应较为明显。【结论】长期施用不同来源粪肥主要带来养分和有机物输入介导的腐熟成分差异,腐熟粪肥可更好地提升土壤DOM腐殖化程度,其中土壤速效养分和DOM腐殖化组分是影响土壤微生物群落结构的主要影响因素,氮循环相关微生物类群对其响应尤为明显,在长期施用畜禽粪肥的土壤中应加以关注。

Effects of Different Nutrient Management Systems and Cultivation Methods on Crop Yield and Soil Fertility

[Objective] The aim was to provide scientific basis for improving the middle and low yielding fields fertility and farmland productivity. [Method] A field experiment was carried out to study the effects of different management practices （including nutrient management systems and cultivation methods） on crop yield and soil fertility in winter wheat/summer maize rotation system. [Result] The crop yield in the treatment of the high yield and high efficiency system was remarkably higher than farmer conventional management practice. After five crop seasons experiment, the contents of soil organic matter for high yield and high efficiency system increased 2.72-3.01 g/kg, and that of soil total nitrogen increased 0.12-0.16 g/kg, the soil Olsen-P increased 5.2 mg/kg and the soil available K （NH4OAC-K） increased about 37.8 mg/kg. [Conclusion] Considering the yield and soil fertility comprehensively, the management system of high yield and high efficiency could effectively increase the crop yield and improve the soil fertility.

Effects of Different Cropping Systems on Soil Quality

[Objective] The aim was to explore the soil properties change in the process of field crops into greenhouse vegetables and their possible adverse effects on the environment.[Method] Fertilization,irrigation,crop growth conditions and soil quality changes in the major cropping systems（greenhouse monoculture,greenhouse rotation and open field rotation）in Chongming Island,Shanghai were investigated from 2007 to 2009.[Result] Different cropping systems significantly affected soil quality.Soil organic carbon,mineral nitrogen,available phosphorus and exchangeable potassium content increased in greenhouse cultivation compared to open field rotation due to excessive application of irrigation and fertilizers.Soil pH values decreased by 0.6 and 0.4 in the greenhouse monoculture and greenhouse rotation,respectively,while did not significantly change in open field rotation.Meanwhile,greenhouse cultivation significantly increased soil EC,and NO-3 and Cl-contents,which showed an increasing trend with the planting years increase.However,the salt content changes were not significant in open field rotation.[Conclusion] High cropping index,excessive fertilizer input and inappropriate fertilization and irrigation resulted in greenhouse soil quality degradation due to weak farmer＇s skill and local extension services.

Effect of Different Fertilization Practices on Yield of a Wheat-Maize Rotation and Soil Fertility

A 15-year field experiment was carried out in Henan Province, China, to study the effects of different fertilization practices on yield of a wheat-maize rotation. Fertilizers tested contained N alone (N), N plus P (NP) or plus P and K (NPK), all with or without manure (M). Different long-term fertilization practices affected the yields under the rotation system of wheat and maize differently and the effects on yields was in a general trend of MNPK＞MNP＞MN＞NPK＞NP＞M＞N＞the control. The average contribution rate of soil fertility to the highest yield was 37.9%, and the rest 62.1% came from fertilizer applications. The yield effects of the chemical fertilizers were in the order of N＞P＞K and were increased by application of manure.Balanced fertilization with multielement chemical fertilizers and manure can be effective in maintaining growth in agricultural production. Combined application of chemical fertilizer and organic manure also increased the content of soil organic matter.

Combining Ridge with No-Tillage in Lowland Rice-Based Cropping System: Long-Term Effect on Soil and Rice Yield

A tillage method of combining ridge with no-tillage (RNT) was employed in lowland rice-based cropping system to study the long-term effects of RNT on soil profile pattern, soil water stable aggregate distribution, nutrients stratification and yields of rice and post-rice crops. After flooded paddy field (FPF) was practiced with RNT for a long time, soil profile changed from G to A-P-G, and horizon G was shifted to a deeper position in the profile. Also the proportion of macroaggregate (> 2 mm) increased, whereas the proportion of silt and clay (< 0.053 mm) decreased under RNT, indicating a better soil structure that will prevent erosion. RNT helped to control leaching and significantly improved total N, P, K and organic matter in soil. The highest crop yields were found under RNT system every year, and total crop yields were higher under conventional paddy-upland rotation tillage (CR) than under FPF, except in 2003 and 2006 when serious drought occurred. RNT was proven to be a better tillage method for lowland rice-based cropping system.

Effect of potassium on soil conservation and productivity of maize/cowpea based crop rotations in the north-west Indian Himalayas

Plots under conservation tillage may require higher amount of potassium(K) application for augmenting productivity due to its stratification in upper soil layers, thereby reducing K supplying capacity in a medium or long-term period. To test this hypothesis, a field experiment was performed in 2002-2003 and 2006-2007 to study the effect of K and several crop rotations on yield, water productivity, carbon sequestration, grain quality, soil K status and economic benefits derived in maize(Zea mays L)/cowpea(Vigna sinensis L.) based cropping system under minimum tillage(MT). All crops recorded higher grain yield with a higher dose of K(120 kg K2 O ha-1) than recommended K(40 kg K2 O ha-1). The five years' average yield data showed that higher K application(120 kg K2 O ha-1) produced 16.4%(P<0.05)more maize equivalent yield. Cowpea based rotation yielded 14.2%(P<0.05) higher production than maize based rotation. The maximum enhancement was found in cowpea-mustard rotation. Relationship between yield and sustainable indices revealed that only agronomic efficiency of fertilizer input was significantly correlated with yield. Similarly, higherdoses of K application not only increased the water use efficiency(WUE) of all crops, but also reduced runoff and soil loss by 16.5% and 15.8% under maize and 23.3% and 19.7% under cowpea cover, respectively. This study also revealed that on an average 16.5% of left over carbon input contributed to soil organic carbon(SOC). Here, cowpea based rotation with the higher K application increased carbon sequestration in soil. Potassium fertilization also significantly improved the nutritional value of harvested grain by increasing the protein content for maize(by 9.5%) and cowpea(by 10.6%). The oil content in mustard increased by 5.0% and 6.0% after maize and cowpea, respectively. Net return also increased with the application of the higher K than recommended K and the trend was similar to yield. Hence, the present study demonstrated the potential yield and profit gains along with resource conservation in the Indian Himalayas due to annual additions of higher amount of K than the recommended dose. The impact of high K application was maximum in the cowpea-mustard rotation.

Fertilization for High Yields in Corn-Sweet Potato-Wheat Rotation: A Systematic Approach to Nutrient Limiting Factors of Soils in Chongqing, China

A systematic approach was adopted to investigate the nutrient limiting factors in gray-brown purple soils and yellow soils derived from limestone in Chongqing, China, to study balanced fertilization for corn, sweet potato and wheat in rotation. The results showed that N, P and K were deficient in both soils, Cu, Mn, S andZn in the gray-brown purple soils and Ca, Mg, Mo and Zn for the yellow soils. Balanced fertilizer application increased yields of corn, sweet potato and wheat by 28.4%, 28.7% and 4.4%, respectively, as compared to the local farmers' practice. The systematic approach can be considered as one of the most efficient and reliable methods in fertility study.

The Effect of Crop Rotation on Soil Nematode Community Composition in a Greenhouse

[Objective] The aim was to identify changes in a nematode community in response to crop rotation and to determine the appropriate catch crop for a greenhouse. [Method] The experiment was carried out in a typical 6-year-old greenhouse,in which cucumber crops were cultivated twice each year(in spring and autumn),and catch crops were planted in summer. The total number of nematodes was counted and nematode community indices were calculated after collecting soil samples in different stages. [Result] Total nematode abundance was decreased in the soils of catch crop in contrast with former crops(cucumber crops). The abundance of the nematode community was reduced in the treatment of crop rotation compared to the soils of catch crop. In addition, the number of nematode taxa was significantly reduced by the treatment of crown daisy compared to the treatments of following crops. Crop rotation regulated the functional composition of the nematode community by increasing the omnivores-predators functional group and decreasing the relative abundance of root herbivores. [Conclusion] These results indicate that crop rotation affects the nematode community in abundance, diversity and functional composition of the nematode community and crown daisy can be served as the most appropriate catch crop in the greenhouse.

Numerical simulation of tire/soil interaction using a verified 3D finite element model

The compaction and stress generation on terrain were always investigated based on empirical approaches or testing methods for tire/soil interaction.However,the analysis should be performed for various tires and at different soil strengths.With the increasing capacity of numerical computers and simulation software,finite element modeling of tire/terrain interaction seems a good approach for predicting the effect of change on the parameters.In this work,an elaborated 3D model fully complianning with the geometry of radial tire 115/60R13 was established,using commercial code Solidwork Simulation.The hyper-elastic and incompressible rubber as tire main material was analyzed by Moony-Rivlin model.The Drucker-Prager yield criterion was used to model the soil compaction.Results show that the model realistically predicts the laboratory tests outputs of the modeled tire on the soft soil.

Nitrogen Recoveries and Yields Improvement in Cowpea sorghum and Fallow sorghum Rotations in West Africa Savannah

The effects of previous cowpea （Vignaunguiculata） and annual fallow on N recoveries, succeeding sorghum yields and soil properties were studied using a 5-year-old （1995-1999） field experiment at Kouar6 （11°59′ North, 0°19′ West and 850 m altitude） in Burkina Faso. A 3 4 factorial design in a split plot arrangement with three rotation treatments and four fertilizer treatments was used. Total N uptake by succeeding sorghum increased from 26 kg N ha~ in mono cropping of sorghum to 31 and 48 kg N ha~ when sorghum was rotated with fallow or cowpea respectively. Nitrogen derived from fertilizer increased from 10% in mono cropping of sorghum to 22% and 26% when sorghum was rotated with fallow or cowpea respectively. While fallow did not increase N derived from soil, cowpea doubled the quantity of N derived from soil （Ndfs）. Sorghum grain yields increased from 75% and 100% when sorghum was rotated with fallow or cowpea respectively. All rotations treatments decreased soil organic C and N but soil organic C was the highest in fallow-sorghum rotation. It was concluded that cowpea-sorghum rotation was more effective than fallow-sorghum rotation and five management options were suggested to improve traditional system productivity.

保护地生产条件下的土壤退化问题及其防治对策

通过对保护地土壤退化问题的综合分析可知,退化的根本原因在于:①迄今为止保护地生产上尚未建立完善的耕作制度,因此不能很好地解决保护地轮休时的土壤环境问题。②市场的需求与保护地的轮作没有统一起来,连作造成营养元素的失衡、土传病虫害的发生与蔓延。③肥料的盲目过量使用降低了肥料的利用率,在土壤中积累了大量的盐分,加剧了土壤的次生盐渍化。④多数保护地至今仍沿用大水漫灌的灌溉方法,这样不仅浪费大量的水资源,也是土壤次生盐渍化产生的重要原因之一。针对上述问题,提出对策如下:首先在保护地轮休前大力推行机械方耕、客土、排土等耕作方法,使土壤得到充分的修复。其次,将市场需求与保护地轮作有机地结合起来,建立适宜于保护地的土壤轮作制度。第三,以有机食品生产的技术标准为依据,实施因土、因作物、因设施的运行情况,统一调配,科学施肥。第四,建立健全保护地的节水灌溉制度和节水技术体系,将节约水资源与改善保护地环境统一起来。通过实施上述综合措施来提高保护地的持续生产能力。

Soil Enzyme Activities and Organic Matter Composition Affected by 26 Years of Continuous Cropping

The study was to determine the long-term effects of subtropical monoculture and rotational cropping systems and fertilization on soil enzyme activities and soil C, N, and P levels. Cropping systems included continuous sorghum(Sorghum bicolor L.), cotton(Gossypium hirsutum L.), corn(Zea mays L.), and cotton/sorghum rotations after 26 years of treatment imposition. Soil under continuous sorghum and continuous corn had 15% and 11%, respectively, greater C concentrations than soil under continuous cotton.Organic C was 10% higher at 0–7.5 cm than at 7.5–15 cm. Total N followed similar trends with soil depth as organic C. Continuous sorghum had 19% higher total N than other crop species and rotations. With fertilization, continuous cotton had the highest total P at 0–7.5 cm and sorghum had the highest at 7.5–15 cm. Soil total P was 14% higher at 0–7.5 than at 7.5–15 cm, and fertilization increased 15% total P compared to unfertilized soil. Arylsulfatase, alkaline phosphatase, and β-d-glucosidase activity were the highest for sorghum and the lowest for cotton. Rotation increased enzyme activities compared to continuous cotton but not for continuous sorghum. Of all crop species and rotations, continuous cotton generally showed the lowest levels of organic matter and enzyme activities after 26 years. Fertilization significantly increased the yields for all cropping systems, but rotation had no significant effect on either sorghum or cotton lint yield compared to each crop grown in monoculture. Long-term cropping did not increase soil organic matter levels beyond short-term gains, indicating the difficulty in promoting C sequestration in subtropical soils.

Sorption and Leaching Potential of Isoproturon and Atrazine in Low Organic Carbon Soil of Pakistan Under a Wheat-Maize Rotation

Pesticide leaching is a great threat in low organic carbon soils when subjected to improper irrigation scheduling.Limited data are available on the sorption and leaching potential of pesticides in agricultural soils of Pakistan with low soil organic carbon(SOC).Lysimeter and field studies were conducted with and without manure application at two irrigation levels in a wheat-fallowmaize rotation in Faisalabad,Punjab,Pakistan.Isoproturon was applied to wheat 55 d after sowing at 1 kg active ingredient(a.i.)ha^(-1),while atrazine was sprayed on maize 30 d after sowing at 0.774 kg a.i.ha^(-1).Soil was sampled from three depths(0-35,35-70,and 70-110 cm) for the field study and four depths(0-35,35-70,70-115,and 115-160 cm) for the lysimeter study,280 and 65 d after application of isoproturon and atrazine,respectively.The soil-water partition coefficients(K_d) of isoproturon and atrazine ranged from 0.3 to 1.2 and 0.4 to 1.5 L kg^(-1),respectively,and increased linearly with increase in SOC contents.The organic carbon-normalized soil-water partition coefficient(K_(oc)) of isoproturon and atrazine averaged 246.1 and 184.9 L kg^(-1),respectively,being higher with low spiking concentration.Isoproturon residues measured 280 d after application ranged from 2.1% to 3.6% of the applied mass in the lysimeter study and from 1.5% to 3.1% under field conditions.Atrazine residues 65 d after application ranged from only 0.62% to 0.78% and from 0.88% to 0.82% in the lysimeter and field studies,respectively.The lowest levels of residues for both pesticides were observed with frequent irrigation applied to manure-amended soil.A pesticide leaching risk screening tool,the ground water ubiquity score(GUS),indicated that in the absence of manure under both irrigation levels,isoproturon has a leaching potential(GUS = 2.8),while with the application of manure it has a very low leaching risk.Atrazine GUS ranged from 1.7 to 1.9,indicating a very low risk of leaching.

Effect of Atmospheric CO_2 Enrichment on Soil Respiration in Winter Wheat Growing Seasons of a Rice-Wheat Rotation System

Studies on the effect of elevated CO2 on C dynamics in cultivated croplands are critical to a better understanding of the C cycling in response to climate change in agroecosystems. To evaluate the effects of elevated CO2 and different N fertilizer application levels on soil respiration, winter wheat （Triticum aestivum L. cv. Yangmai 14） plants were exposed to either ambient CO2 or elevated CO2 （ambient [CO2] ＋ 200 μmol mol-1）, under N fertilizer application levels of 112.5 and 225 kg N ha-1 （as low N and normal N subtreatments, respectively）, for two growing seasons （2006-2007 and 2007-2008） in a rice-winter wheat rotation system typical in China. A split-plot design was adopted. A root exclusion method was used to partition soil respiration （RS） into heterotrophic respiration （RH） and autotrophic respiration （RA）. Atmospheric CO2 enrichment increased seasonal cumulative RS by 11.8% at low N and 5.6% at normal N when averaged over two growing seasons. Elevated CO2 significantly enhanced （P 〈 0.05） RS （12.7%）, mainly due to the increase in RH （caused by decomposition of larger amounts of rice residue under elevated CO2） during a relative dry season in 2007-2008. Higher N supply also enhanced RS under ambient and elevated CO2. In the 2007-2008 season, normal N treatment had a significant positive effect （P 〈 0.01） on seasonal cumulative RS relative to low N treatment when averaged across CO2 levels （16.3%）. A significant increase in RA was mainly responsible for the enhanced RS under higher N supply. The correlation （r2） between RH and soil temperature was stronger （P 〈 0.001） than that between RS and soil temperature when averaged across all treatments in both seasons. Seasonal patterns of RA may be more closely related to the plant phenology than soil temperature. The Q10 （the multiplier to the respiration rate for a 10 ℃ increase in soil temperature） values of RS and RH were not affected by elevated CO2 or higher N supply. These results mainly suggested that the increase in RS at elevated CO2 depended on the input of rice residue, and the increase in RS at higher N supply was due to stimulated root growth and concomitant increase in RA during the wheat growing portion of a rice-winter wheat rotation system.

Soil Microbiological and Biochemical Properties as Affected by Different Long-Term Banana-Based Rotations in the Tropics

Soil microbiological and biochemical properties under various field crop rotations such as grains, pastures and vegetables have been studied intensively under short-term period. However, there is limited information about the influence of banana-based rotations on soil organic C, total N(TN), microbial biomasses and enzyme activities under long-term crop rotations. A field experiment arranged in a randomized complete block design with three replicates was carried out at the Wanzhong Farm in Ledong(18?37′–18?38′N, 108?46′–108?48′E), Hainan Province, China, to compare the responses of these soil parameters to long-term(10-year) banana(Musa paradisiaca)-pineapple(Ananas) rotation(AB), banana-papaya(Carica) rotation(BB) and banana monoculture(CK) in a conventional tillage system in the Hainan Island. Soil p H, total organic C(TOC), dissolved organic C(DOC), TN, total P(TP) and available P(AP) were found to be significantly higher(P < 0.01) in AB and BB than CK at 0–30 cm soil depth. Microbial biomass C(MBC) and N(MBN) were observed 18.0%–35.2% higher in AB and 8.6%–40.5% higher in BB than CK at 0–30 cm. The activities of urease(UA), invertase(IA), dehydrogenase(DA) and acid phosphatase(APA) showed a mean of 21.5%–59.6% increase in AB and 26.7%–66.1% increase in BB compared with CK at 0–30 cm. Higher p H, TOC and DOC at 0–10 and 10–20 cm than at 20–30 cm were obtained despite of the rotations. Soil MBC and MBN and activities of UA, IA and DA decreased markedly(P < 0.01) with increasing soil depth in the different rotation soils as well as the monoculture soil. In general, soil microbial biomass and enzymatic activities were more sensitive to changes in banana-based rotations than soil chemical properties, and consequently they were well-established as early indicators of changes due to crop rotations in the tropics.

Responses of Soil Nematode Abundance and Diversity to Long-Term Crop Rotations in Tropical China

A field experiment was carried out from 2003 to 2013 in the Wanzhong Farm of the Hainan Island, China, to determine the effects of two long-term banana rotations on the abundance and trophic groups of soil nematode communities in the island. The experiment was set out as a randomized complete block design with three replications of three treatments： banana-pineapple rotation （AB）, banana-papaya rotation （BB） and banana monoculture （CK） in a conventional tillage system. Soil samples were taken at depths of 0-10, 10-20 and 20-30 cm, and nematodes were extracted by a modified cotton-wool filter method and identified to the genus level. Nematode ecological indices of Shannon-Weaver diversity （Ht）, dominance index （A）, maturity index （MI）, plant parasite index （PPI）, structure index （SI）, enrichment index （EI）, and channel index （CI） were calculated. A total of 28 nematode genera with relative abundance over 0.1% were identified, among which Tylenchus and Paratylenchus in the AB, Thonus in the BB, Tylenchus and Helicotylenchus in the CK were the dominant genera. The rotation soils favored bacterivores, fungivores and omnivores-predators with high colonizer-persister （c-p） values. Soil food web in the rotation systems was highly structured, mature and enriched as indicated by SI, MI and EI values, respectively. Higher abundance of bacterivores and lower values of CI suggested that the soil food web was dominated by a bacterial decomposition pathway in rotation soils. Nematode diversity was much higher after a decade of rotation. Soil depth had significant effects on the abundance of soil nematodes, but only on two nematode ecological indices （λ and MI）.

Fertilization regimes affect the soil biological characteristics of a sudangrass and ryegrass rotation system

The sudangrass (Sorghum sudanense) and ryegrass (Lolium multiflorum L.) rotation is an intensive and new cropping system in Central China.Nutrient management practices in this rotation system may influence soil fertility,the important aspects of which are soil biological properties and quality.As sensitive soil biological properties and quality indicators,soil microbial community activity,microbial biomass,enzyme activities,soil organic matter (SOM) and total N resulting from different fertilization regimes in this rotation system were studied through a four-year field experiment from April 2005 to May 2009.Treatments included control (CK),fertilizer phosphorus and potassium (PK),fertilizer nitrogen and potassium (NK),fertilizer nitrogen and phosphorus (NP) and a fertilizer nitrogen,phosphorus and potassium combination (NPK).Soil microbial community activities in the NK,NP and NPK treatments were significantly lower than those in the CK and PK treatments after the sudangrass and ryegrass trial.The highest microbial biomass C,microbial biomass N,SOM,total N,sucrase and urease activities were found in the NPK treatment,and these soil quality indicators were significantly higher in the NK,NP and NPK treatments than in the PK and CK treatments.Soil microbial biomass and enzyme activities were positively associated with SOM in the sudangrass and ryegrass rotation system,indicating that fertilization regimes,especially N application,reduced microbial community activity in the soil.Proper fertilization regimes will increase microbial biomass,enzyme activity and SOM and improve soil fertility.