期刊文献+
共找到42篇文章
< 1 2 3 >
每页显示 20 50 100
武汉造船工程学会学轮机学组委会召开“船机技术政策”座谈会
1
作者 祥荣 《船海工程》 1980年第3期175-176,共2页
武汉造船工程学会学委会船机技术政策的座谈会于六月二十四日在武汉水运工程学院召开。长航科技处、长航科研所、长航机务处、长航青山船厂、长江船舶设计院、长江航道局、海军工程学院、华中工学院、武汉水运工程学院及武汉造船学会学... 武汉造船工程学会学委会船机技术政策的座谈会于六月二十四日在武汉水运工程学院召开。长航科技处、长航科研所、长航机务处、长航青山船厂、长江船舶设计院、长江航道局、海军工程学院、华中工学院、武汉水运工程学院及武汉造船学会学委会秘书组等十个单位的二十余位专家学者出席了会议。中国造船学会轮机学术委员会主任委员、上海交通大学教授李铭慰同志也出席了会议并讲了话。 展开更多
关键词 武汉水运工程 座谈会 轮机学 武汉造船工程 委会 船机 长江船舶设计院 长航 技术政策
下载PDF
Kinematical system of breadth cam profile design 被引量:1
2
作者 SHIN Joong-ho 李琳 +1 位作者 YANG Hyun-dai KWON Soon-man 《Journal of Central South University》 SCIE EI CAS 2011年第2期451-457,共7页
The design solutions for breadth cam mechanism was presented. The main topics of the shape design for breadth cam was to calculate the coordinate at each contact point to determine the cam profile. The proposed method... The design solutions for breadth cam mechanism was presented. The main topics of the shape design for breadth cam was to calculate the coordinate at each contact point to determine the cam profile. The proposed method according to velocity and geometric relationships of instant velocity centers can easily determine each contact point at any instant moment. The cam profile was defined by contouring of the contact points. And also a program was developed by using Microsoft Visual C++ program,which can quickly and easily draw a 2D cam profile through the displacement diagram. Finally,the program was used to confirm the accuracy on the breadth cam profile design by computer animation graphically. 展开更多
关键词 cam profile breadth cam reciprocating follower oscillating follower instant velocity center
下载PDF
Thermodynamic Analysis of Alternative Marine Fuels for Marine Gas Turbine Power Plants 被引量:1
3
作者 Mohamed M. El Gohary Nader R. Ammar 《Journal of Marine Science and Application》 CSCD 2016年第1期95-103,共9页
The marine shipping industry faces challenges to reduce engine exhaust emissions and greenhouse gases (GHGs) from ships, and in particular, carbon dioxide. International regulatory bodies such as the International M... The marine shipping industry faces challenges to reduce engine exhaust emissions and greenhouse gases (GHGs) from ships, and in particular, carbon dioxide. International regulatory bodies such as the International Maritime Organization and National Environmental Agencies of many countries have issued rules and regulations to drastically reduce GHG and emissions emanating from marine sources. This study investigates the possibility of using natural gas and hydrogen as alternative fuels to diesel oil for marine gas turbines and uses a mathematical model to assess the effect of these alternative fuels on gas turbine thermodynamic performance. Results show that since natural gas is categorized as a hydrocarbon fuel, the thermodynamic performance of the gas turbine cycle using natural gas was close to that of the diesel case. However, the gas turbine thermal efficiency was found to be slightly lower for natural gas and hydrogen fuels compared to diesel fuel. 展开更多
关键词 ship emissions natural gas HYDROGEN gas turbine thermodynamic analysis gas turbine power plants greenhouse gases (GHGs)
下载PDF
Numerical Study of a Novel Procedure for Installing the Tower and Rotor Nacelle Assembly of Offshore Wind Turbines Based on the Inverted Pendulum Principle 被引量:10
4
作者 Wilson Guachamin Acero Zhen Gao Torgeir Moan 《Journal of Marine Science and Application》 CSCD 2017年第3期243-260,共18页
Current installation costs of offshore wind turbines(OWTs) are high and profit margins in the offshore wind energy sector are low, it is thus necessary to develop installation methods that are more efficient and pract... Current installation costs of offshore wind turbines(OWTs) are high and profit margins in the offshore wind energy sector are low, it is thus necessary to develop installation methods that are more efficient and practical. This paper presents a numerical study(based on a global response analysis of marine operations) of a novel procedure for installing the tower and Rotor Nacelle Assemblies(RNAs) on bottom-fixed foundations of OWTs. The installation procedure is based on the inverted pendulum principle. A cargo barge is used to transport the OWT assembly in a horizontal position to the site, and a medium-size Heavy Lift Vessel(HLV) is then employed to lift and up-end the OWT assembly using a special upending frame. The main advantage of this novel procedure is that the need for a huge HLV(in terms of lifting height and capacity) is eliminated. This novel method requires that the cargo barge is in the leeward side of the HLV(which can be positioned with the best heading) during the entire installation. This is to benefit from shielding effects of the HLV on the motions of the cargo barge, so the foundations need to be installed with a specific heading based on wave direction statistics of the site and a typical installation season. Following a systematic approach based on numerical simulations of actual operations, potential critical installation activities, corresponding critical events, and limiting(response) parameters are identified. In addition, operational limits for some of the limiting parameters are established in terms of allowable limits of sea states. Following a preliminary assessment of these operational limits, the duration of the entire operation, the equipment used, and weather-and water depth-sensitivity, this novel procedure is demonstrated to be viable. 展开更多
关键词 offshore wind turbine installation crane vessel shielding effects critical events limiting parameters inverted pendulum allowable sea states
下载PDF
Parametric geometry representations for wind turbine blade shapes 被引量:3
5
作者 Chen Jin Zhang Shiqiang +1 位作者 Hou Yi Eecen Peter 《High Technology Letters》 EI CAS 2011年第3期325-332,共8页
Based on the Joukowsky transformation and Theodorsen method, a novel parametric function (shape function) for wind turbine airfoils has been developed. The airfoil design space and shape control equations also have ... Based on the Joukowsky transformation and Theodorsen method, a novel parametric function (shape function) for wind turbine airfoils has been developed. The airfoil design space and shape control equations also have been studied. Results of the analysis of a typical wind turbine airfoil are shown to illustrate the evaluation process and to demonstrate the rate of convergence of the geometric characteristics. The coordinates and aerodynamic performance of approximate airfoils is rapidly close to the baseline airfoil corresponding to increasing orders of polynomial. Comparison of the RFOIL prediction and experimental results for the baseline airfoil generally show good agreement. A universal method for three-dimensional blade integration-" Shape function/Distribution function" is presented. By changing the parameters of shape function and distribution functions, a three dimensional blade can be designed and then transformed into the physical space in which the actual geometry is defined. Application of this method to a wind turbine blade is presented and the differences of power performance between the represented blade and original one are less than 0. 5%. This method is particularly simple and convenient for bodies of streamline forms. 展开更多
关键词 wind turbine AIRFOILS aerodynamic convergence shape function/ distribution function orders of polynomial (OP)
下载PDF
Coupling simulation of fluid structure interaction in the stirred vessel with a pitched blade turbine
6
作者 Yangyang Liang Zhengming Gao +2 位作者 Dai'en Shi Wanli Zhao Ziqi Cai 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2018年第5期922-929,共8页
The interaction between fluid and a down-pumping pitched blade turbine fixed with a flexible shaft in the stirred vessel, as a typical fluid structure interaction phenomenon, was simulated by coupling the Computationa... The interaction between fluid and a down-pumping pitched blade turbine fixed with a flexible shaft in the stirred vessel, as a typical fluid structure interaction phenomenon, was simulated by coupling the Computational Fluid Dynamics and Computational Structural Dynamics. Based on the verification of the simulated impeller torque and dimensionless shaft bending moment with experimental result, the dimensionless shaft bending moment and various loads acting on impeller(including lateral force, axial force and bending moment) were discussed in detail. By separating and extracting the fluid and structural components from those loads, the results show that the shaft bending moment mainly results from the lateral force on impeller although the axial force on impeller is much larger. The impeller mass imbalance increases the shaft bending moment and the lateral force on impeller, but has little influence on the axial force and bending moment acting on impeller. The dominant frequencies of impeller forces are macro-frequency, speed frequency and blade passing frequency, and are associated with the impeller mass imbalance. 展开更多
关键词 Fluid structure interaction Shaft bending moment Impeller lateral force Impeller axial force Bending moment on impeller
下载PDF
Direct and Inverse Kinematic Analysis of a Leg-wheeled Passive Wheel Mobile Robot - Ice-skater Robot
7
作者 宋立博 张家梁 +1 位作者 吕恬生 陆顺寿 《Journal of Donghua University(English Edition)》 EI CAS 2003年第3期27-31,共5页
A new passive wheel type of leg-wheeled mobile robot based on rolling principle was introduced. To enhance the stability and maintain vertical to the ground of wheels, four passive wheels were installed at the end of ... A new passive wheel type of leg-wheeled mobile robot based on rolling principle was introduced. To enhance the stability and maintain vertical to the ground of wheels, four passive wheels were installed at the end of four legs respectively and parallel mechanisms were used as legs. And an inertia coordinate system and a robot coordinate system were established, the related kinematic equation of the robot was gotten according to some assumptions after the configuration or the posture of wheels and legs was analyzed. At the same time, the turning conditions of the robot were also obtained. Based on the motion principle, the VSS-based logic control system was designed and the skating straight experiments and the turning experiments were conducted. And some conclusions were drawn. 展开更多
关键词 Ice-skater Robot hybrid mobile robot kinematic analysis VSS-based logic control
下载PDF
Modeling and Optimization of the Steam Turbine Network of an Ethylene Plant 被引量:4
8
作者 李泽秋 赵亮 +1 位作者 杜文莉 钱锋 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第5期520-528,共9页
In this paper,we developed a hybrid model for the steam turbines of a utility system,which combines an improved neural network model with the thermodynamic model.Then,a nonlinear programming(NLP) model of the steam tu... In this paper,we developed a hybrid model for the steam turbines of a utility system,which combines an improved neural network model with the thermodynamic model.Then,a nonlinear programming(NLP) model of the steam turbine network is formulated by utilizing the developed steam turbine models to minimize the total steam cost for the whole steam turbine network.Finally,this model is applied to optimize the steam turbine network of an ethylene plant.The obtained results demonstrate that this hybrid model can accurately estimate and evaluate the performance of steam turbines,and the significant cost savings can be made by optimizing the steam turbine network operation at no capital cost. 展开更多
关键词 hybrid model steam turbine network utility system economic objective
下载PDF
Numerical Simulation and Kinetic Analysis of Turbine Sail
9
作者 Hu Yihuai Wang Taiyou Luan Yongli 《Journal of Energy and Power Engineering》 2017年第2期127-134,共8页
This paper firstly introduces the structure and working principle of turbine sail. Numerical model of a turbine sail is established with Gambit software. The aerodynamic characteristics of the turbine sail are describ... This paper firstly introduces the structure and working principle of turbine sail. Numerical model of a turbine sail is established with Gambit software. The aerodynamic characteristics of the turbine sail are described with RNG k-e turbulence model and the numerical simulation is carded out with Fluent software. The influence of sail's structure is analyzed including plate, separation type and height/width ratio. The lift coefficients and drag coefficients of the simulated turbine sail are calculated under different rotation angles, suction intensity and separation plate position. The calculated results are compared with the wind tunnel experimental results, which verifies the feasibility of the numerical results and establishes a foundation for the optimal design of turbine sails. 展开更多
关键词 Turbine sail aerodynamic characteristics fluent calculation wind tunnel test.
下载PDF
Numerical Hydrodynamics Study Around Turbine Array of Tidal Stream Farm in Zhoushan, China 被引量:1
10
作者 YU Zhiwen ZHANG Jisheng +2 位作者 ZHAI Yanyan ZHANG Tiantian ZHENG Jinhai 《Journal of Ocean University of China》 SCIE CAS CSCD 2017年第4期703-708,共6页
In recent decades,great efforts have been made to efficiently explore tidal stream energy due to its unique advantages of easy prediction and great potential.China recently launched a national tidal stream farm demons... In recent decades,great efforts have been made to efficiently explore tidal stream energy due to its unique advantages of easy prediction and great potential.China recently launched a national tidal stream farm demonstration project in the waterway between Putuoshan and Hulu Islands in the Zhoushan area.Before deployment of the turbine array,it is necessary to understand the hydrodynamic changes associated with the construction of a turbine array.In this study,we developed a depth-averaged hydrodynamics model that solves the shallow water governing equations to simulate the tidal hydrodynamics around the Zhoushan Archipelago.The simulation results agree with field data in terms of the water elevation and stream velocity.We considered two types of turbine arrays in this study and investigated their impacts on the local hydrodynamics.In general,the stream velocity in the northern and southern areas is reduced due to the power take-off of the turbine array,whereas stream velocity in the western and eastern areas is slightly increased due to the blockage impact of the turbine array. 展开更多
关键词 tidal stream farm turbine array tidal hydrodynamics numerical simulation demonstration project
下载PDF
Adsorption Characteristics of Activated Carbon Derived from Scrap Tires for Malachite Green:Influence of Small Organics 被引量:2
11
作者 李丽 刘玉静 +2 位作者 王佳平 刘双喜 朱坦 《Transactions of Tianjin University》 EI CAS 2013年第6期425-429,共5页
The influence of small organics on the adsorption characteristics of activated carbon produced from industrial pyrolytic tire char(APTC)for malachite green(MG) was investigated by a batch method. Phenol was chosen as ... The influence of small organics on the adsorption characteristics of activated carbon produced from industrial pyrolytic tire char(APTC)for malachite green(MG) was investigated by a batch method. Phenol was chosen as the representative of small organics. The effects of phenol on adsorption equilibrium, kinetics and thermodynamics were studied systematically. The results indicate that APTC is a potential adsorbent for MG. The presence of phenol decreases the adsorption capacity of APTC for MG, but improves the rate of adsorption, while the adsorption characteristics, such as equilibrium, kinetics and thermodynamics are not affected by phenol. The adsorption equilibrium data follow Langmuir isotherm and the kinetic data are well described by the pseudo-second-order kinetic model. The adsorption process follows intra-particle diffusion model and the adsorption rate is determined by more than one process. Thermodynamic study shows that the adsorption is an endothermic and spontaneous physisorption process. 展开更多
关键词 activated carbon scrap tire pyrolytic tire char adsorption malachite green phenol
下载PDF
Impeller modeling and analysis based on UG NX/KF and Fluent 被引量:5
12
作者 王凌云 黄红辉 Rae W.West 《Journal of Central South University》 SCIE EI CAS 2012年第12期3430-3434,共5页
Parametric modeling of the impeller which drove a small wind device was built by knowledge fusion technology.NACA2410 airfoil blade was created by KF language.Using technology of UG/KF secondary development for the au... Parametric modeling of the impeller which drove a small wind device was built by knowledge fusion technology.NACA2410 airfoil blade was created by KF language.Using technology of UG/KF secondary development for the automatic modeling of wind turbine blade,the program can read in the airfoil data files automatically and the impeller model entity can be generated automatically.In order to modify the model,the aerodynamic characteristics of the impeller were analyzed for getting aerodynamic parameters by Fluent.The maximum force torch and best parameters of impeller were calculated.A physical prototype impeller was manufactured and the correctness of the design was verified,and the error of force torch between simulation and experimental results is about 10%.Parameterization design of the impeller model greatly improves the efficiency of modeling and flexibility of the CAD system. 展开更多
关键词 knowledge fusion airfoil wind turbine blade parametric design CAD/CAE
下载PDF
Research on pattern recognition for marine steam turbine rotor axis orbit
13
作者 ZHANG Yan, YANG Zhi-da, XIA Hong School of Power and Nuclear Energy Engineering, Harbin Engineering University,Harbin 150001, China 《Journal of Marine Science and Application》 2003年第1期45-48,52,共5页
The structure,function and recognition method of an axis orbit auto-recognizing system are presented in this paper.In order to make the best use of information of format and dynamic characteristics of marine steam tur... The structure,function and recognition method of an axis orbit auto-recognizing system are presented in this paper.In order to make the best use of information of format and dynamic characteristics of marine steam turbine axis orbit,the structure and functions or neural network are applied to this system,which can be used to auto-recognize axis orbit of the system turbine rotor using BP neural network. 展开更多
关键词 axis orbit pattern recognition neural network
下载PDF
Fluent-based numerical simulation of flow centrifugal fan 被引量:2
14
作者 LI Xian-zhang 《Journal of Coal Science & Engineering(China)》 2011年第2期198-200,共3页
Testing centrifugal fan flow field by physical laboratory is difficult because the testing system is complex and the workload is heavy, and the results observed by naked-eye deviates far from the actual value. To addr... Testing centrifugal fan flow field by physical laboratory is difficult because the testing system is complex and the workload is heavy, and the results observed by naked-eye deviates far from the actual value. To address this problem, the computational fluid dynamics software FLUENT was applied to establish three-dimensional model of the centrifugal fan. The numeral model was verified by comparing simulation data to experimental data. The pressure centrifugal fan and the speed changes in distribution in centrifugal fan was simulated by computational fluid dynamics soft-ware FLUENT. The simulation results show that the gas flow velocity in the impeller increases with impeller radius increase. Static pressure gradually increases when gas from the fan access is imported through fan impeller leaving fans. 展开更多
关键词 centrifugal fan numerical simulation flow field FLUENT software
下载PDF
Mathematical model of slotting manufacture and simulation for cosine gears
15
作者 王建 罗善明 徐攀 《Journal of Central South University》 SCIE EI CAS 2014年第3期933-941,共9页
A slotting method for cosine gears was proposed by using the involute cutter based on the conjugate theories of digital surface and gear meshing. The slotting model between the cosine tooth surface represented by disc... A slotting method for cosine gears was proposed by using the involute cutter based on the conjugate theories of digital surface and gear meshing. The slotting model between the cosine tooth surface represented by discrete points and the cutter figuration determined by analytic function was built for their geometrical relationship and conjugate motion during the machining process. Conjugate points in the cutter section corresponding to the discrete points on the cosine gear tooth surface and the conjugate movements were solved. By using the flexible control of the CNC procedure, the active control of the cutting trace of the slotting cutter was accomplished and the envelop forming of the cosine gear was obtained. Based on the software C++ and Matlab, the computer aided manufacturing(CAM) system of the cosine gear was developed, and the simulation of the slotting process was accomplished. Several examples were provided to illustrate the approach. The result of the simulation demonstrates that the proposed slotting method is feasible. 展开更多
关键词 cosine gear mathemattical model computer simulation slotting manufacture CAM
下载PDF
Improving cam profile design optimization based on classical splines and dynamic model 被引量:8
16
作者 夏必忠 刘新成 +1 位作者 尚欣 任世远 《Journal of Central South University》 SCIE EI CAS CSCD 2017年第8期1817-1825,共9页
Cam profiles play an important part in the performance of cam mechanisms. Syntheses of cam profile designs and dynamics of cam designs are studied at first. Then, a cam profile design optimization model based on the s... Cam profiles play an important part in the performance of cam mechanisms. Syntheses of cam profile designs and dynamics of cam designs are studied at first. Then, a cam profile design optimization model based on the six order classical spline and single DOF(degree of freedom) dynamic model of single-dwell cam mechanisms is developed. And dynamic constraints such as jumps and vibrations of followers are considered. This optimization model, with many advantages such as universalities of applications, conveniences to operations and good performances in improving kinematic and dynamic properties of cam mechanisms, is good except for the discontinuity of jerks at the end knots of cam profiles which will cause vibrations of cam systems. However, the optimization is improved by combining the six order classical spline with general polynomial spline which is the so-called "trade-offs". Finally, improved optimization is proven to have a better performance in designing cam profiles. 展开更多
关键词 cam profile design spline optimization trade-off
下载PDF
A New Control Strategy for Modeling Wind Energy Systems Using Fuzzy Cognitive Maps
17
作者 Peter Groumpos Vaia Gkountroumani 《Journal of Energy and Power Engineering》 2014年第11期1859-1868,共10页
Wind energy is currently a fast-growing interdisciplinary field that encompasses many different branches of engineering and science. Modeling and controlling wind energy systems are difficult and challenging problems.... Wind energy is currently a fast-growing interdisciplinary field that encompasses many different branches of engineering and science. Modeling and controlling wind energy systems are difficult and challenging problems. The basic structure of wind turbines and some wind control system methods are briefly reviewed. The need for using advanced theories from fuzzy and intelligent systems in studying wind energy systems is identified and justified. FCMs (fuzzy cognitive maps) are used to model wind energy systems. Simulation studies are performed and obtained results are discussed. A new mathematical approach has been proposed to model dynamical complex systems, the DYFUKN (dynamic fuzzy knowledge networks). Many open problems in the areas of modeling and controlling wind energy systems are outlined. 展开更多
关键词 MODELING CONTROL energy systems wind generators fuzzy cognitive maps.
下载PDF
Measurement and Simulation of the Flow Field around a Triangular Lattice Meteorological Mast
18
作者 Matthew Stickland Thomas Scanlon +2 位作者 Sylvie Fabre Andrew Oldroyd Detlef Kindler 《Journal of Energy and Power Engineering》 2013年第10期1934-1939,共6页
The international standard IEC 61400-12-1 Wind turbines--Part 12-1: Power performance measurements of electricity producing wind turbines" aims to provide a uniform methodology that will ensure consistency, accuracy... The international standard IEC 61400-12-1 Wind turbines--Part 12-1: Power performance measurements of electricity producing wind turbines" aims to provide a uniform methodology that will ensure consistency, accuracy and reproducibility in the measurement and analysis of power performance by wind turbines. Annex G of this standard provides a methodology for the appropriate arrangement of instruments on the meteorological mast to ensure accurate measurement. For cup anemometers it provides recommendations about their location relative to the mast so that the effect of mast and boom interference on their output may be minimised. These recommendations are given for both tubular masts and lattice masts. This paper compares the flow distortion predicted by the IEC standard and the results of a 3D CFD (computational fluid dynamics) simulation of a triangular lattice mast. Based on the results of wind tunnel and CFD simulation it was found that the flow distortion surrounding the lattice mast was overpredicted by the method suggested in appendix G oflEC61400-12-1. Using the CFD data it was possible to determine, for a range of flow directions and mast heights, the distance from the mast that anemometers would need to be in order to be outside the flow distortion field. 展开更多
关键词 Meteorological mast INTERFERENCE lattice tower flow distortion.
下载PDF
Influence of Adjusting Control Accuracy on Pressure Probe Measurements in Turbo Machines
19
作者 Tobias W. Zimmermanna Oliver Curkovic +1 位作者 Manfred Wirsum Andrew Fowler 《Journal of Energy and Power Engineering》 2016年第9期522-533,共12页
This paper presents a new design of a probe adjusting device intended to position pressure and temperature probes in a flow field. 5-hole, 3-hole and temperature probes can be moved in radial direction and freely rota... This paper presents a new design of a probe adjusting device intended to position pressure and temperature probes in a flow field. 5-hole, 3-hole and temperature probes can be moved in radial direction and freely rotated about their axis. The high actuation accuracy of 3.9 ktm in radial direction and 0.09~ in angular position is validated in a 2-stage-turbine test rig which is installed at the Institute of Power Plant Technology, Steam and Gas Turbines, RWTH Aachen University. To meet the challenge to calculate the efficiency of a turbo machine which is mainly influenced by the temperature, all probe adjusting devices are positioned simultaneously and controlled by the MAS (measuring acquisition system) so that the same radial position in each stage is measured at the same time. For this purpose a new program has been developed to synchronize actuation and measurement. The slim design of 60 mm width allows measurement between the stages of turbo machines with small axial distances between vane and blade. In addition a CFD/FEA shows how the design and combination of materials compensate the thermal expansion of the engine during operation. This allows a minimal safety distance of 0.2 mm between rotor and probe to enable measurement as close to the physical boundary as possible. The actuation accuracy is demonstrated with pressure, temperature and angle distribution plots. It is also shown that the resolution of the measuring points, and therefore the actuation distances, has a large impact on the flow field analysis and should be set as high as possible. However the measuring time has to be taken into account. 展开更多
关键词 PROBE measurement ACCURACY
下载PDF
The expression of Bcl-2 and Bax produced by sub-chronic intoxication with the cyanotoxin Microcystin-LR
20
作者 Zhijian Hu Hua Chen +3 位作者 Jinfa Xue Xiaoyang Zhang Xishun Shi Hong Zou 《The Chinese-German Journal of Clinical Oncology》 CAS 2010年第2期68-72,共5页
Objective:We explored the role and molecular mechanism of Microcystin-LR(MC-LR) in hepatocarcinogenesis.Methods:The two-stage-medium-term theory was applied to the establishment of the animal model.The promoting effec... Objective:We explored the role and molecular mechanism of Microcystin-LR(MC-LR) in hepatocarcinogenesis.Methods:The two-stage-medium-term theory was applied to the establishment of the animal model.The promoting effect of MC-LR on liver tumor was evaluated with the Albert γ-GT methods.During the tumor-promoting course,the effects of MC-LR on the regulation and expression of the Bcl-2 and Bax genes were studied with immunohistochemical technique and RT-PCR.Results:MCLR could enhance the positive reaction of Albert γ-GT(GGT),a preneoplasm marker.The positive reaction rate of GGT in diethylnitrosamine(DEN) + MC group was significantly higher than that in the DEN control group.The protein and RNA expression of Bcl-2 was significantly increased by MC-LR,and that the protein expression of Bax was decreased simultaneously.Conclusion:These results indicate that the MC-LR can inhabit apoptosis through the Bcl-2 and Bax genes.Therefore,we conclude that the expression change of Bcl-2 and Bax genes possibly plays an important role in the promotion of liver tumor by MC-LR. 展开更多
关键词 MICROCYSTIN-LR Albert γ-GT BCL-2 BAX liver cancer
下载PDF
上一页 1 2 3 下一页 到第
使用帮助 返回顶部