软肝再生颗粒联合恩替卡韦抗肝纤维化效果观察

目的评价软肝再生颗粒联合恩替卡韦抗肝纤维化的临床疗效。方法采用开放、非随机的方法纳入慢性乙型肝炎合并肝纤维化患者123例,分恩替卡韦单药组(A组)、软肝再生颗粒联合恩替卡韦组(B组)、复方鳖甲软肝片联合恩替卡韦组(C组)3组。观察治疗前后各组肝功能、凝血功能、肝纤维化指标、肝脏超声(门静脉、脾静脉宽度,脾脏厚度)、Fibro Scan肝脏硬度值(LSM)等指标的变化。结果治疗后,各组患者谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、血清白蛋白(ALB)、凝血酶原时间(PT)均有明显改善;治疗后24周和48周,B组、C组上述指标恢复情况均优于A组(P<0.05,P<0.01)。治疗48周,脾脏厚度缩小程度B组、C组均优于A组(P<0.05)。治疗48周,B组、C组肝纤维化指标及LSM改善程度明显优于A组(P<0.01)。治疗48周,B组与C组各个观察指标比较,差异均无统计学意义(P>0.05)。结论软肝再生颗粒联合恩替卡韦治疗可显著改善患者肝功能,对于慢性乙型肝炎导致的肝纤维化有明显疗效,可抑制肝纤维化及脾肿大进展,与复方鳖甲软肝片联合恩替卡韦效果相当。

辨证与辨病应用软肝再生颗粒治疗慢乙肝肝纤维化疗效对比观察

目的:探讨辨证论治与单纯辨病应用软肝再生颗粒联合恩替卡韦抗肝纤维化的疗效差异。方法:采用开放、非随机的方法纳入慢性乙型肝炎合并肝纤维化患者98例,经中医师辨证为瘀血阻络证(兼或可有肝郁脾虚或肝肾阴虚证)的47例患者作为辨证治疗组(简称辨证组);西医门诊接诊的单纯辨病治疗的患者51例作为辨病治疗组(简称辨病组)。两组患者均采用保肝、对症及综合支持治疗,抗乙肝病毒(HBV)采用恩替卡韦胶囊0.5mg,qd,均在抗病毒治疗的基础上服用我院制剂软肝再生颗粒12g,tid。疗程为48周,分别于治疗前、第24周、第48周比较两组肝功能和凝血功能、肝脏硬度值(LSM)、测量门静脉直径、脾静脉宽度和脾脏厚度,并记录患者的不良反应。结果:治疗后,两组患者ALT、AST、TBIL、ALB、PT均有明显改善;治疗第24周和48周,辨证组上述指标恢复情况均优于辨病组(P<0.01或P<0.05)。治疗48周,门静脉直径、脾静脉宽度和脾脏厚度缩小程度辨证组均优于辨病组(P<0.05)。治疗48周,辨证组肝纤维化指标及LSM改善程度明显优于辨病组(P<0.01)。两组不良反应比较,辨证组明显具有优势。结论:软肝再生颗粒联合恩替卡韦可显著改善患者肝功能,对于慢性乙型肝炎导致的肝纤维化有明显疗效,可抑制肝纤维化及脾肿大进展,辨证论治比辨病治疗疗效更好,不良反应更少,两者比较差异有显著性。

软肝再生颗粒剂药效学研究

目的 :研究软肝再生颗粒剂 ( RGZS)防治慢性肝炎、肝炎肝硬化的作用。方法 :运用大鼠实验性肝硬化模型观察 RGZS对大鼠血清谷丙转氨酶 ( AL T)、谷草转氨酶 ( AST)活性及肝组织中羟脯氨酸 ( Hyp)含量的影响 ,同时观察其对大鼠肝组织形态学影响。结果 :对肝硬化大鼠 AL T、AST及肝组织中 Hyp含量有明显的降低效应。光镜可见能抑制胶原纤维增生、刺激肝细胞修复和再生 ,减轻肝细胞变性和坏死的程度 ,电镜下可见

软肝再生颗粒治疗慢性乙型肝炎肝纤维化临床研究

目的 研究软肝再生颗粒对慢性乙型肝炎血清肝纤维化指标的影响。方法 给予86例慢性乙型肝炎患者软肝再生颗粒(12.5 g/包),1包/次 tid,疗程12个月,动态测定HA、LN、PCⅢ、ⅣC及肝功能。结果 4项血清肝纤维化指标随着治疗时间的延长不断下降,第12个月最明显(与治疗前相比,均P<0.01),与对照组同期相比,均P<0.01,且与肝功能复常率具有很好的相关性。结论 软肝再生颗粒具有很好的保肝、抗纤维化作用。

软肝再生颗粒对慢性肝病患者肝纤维化指标的影响

目的 :观察软肝再生颗粒对慢性肝病患者肝纤维化指标的疗效。方法 :选择慢性乙型肝炎、肝炎肝硬变患者 135例为观察对象 ,治疗组 76例 ,服软肝再生颗粒每次 12 g ,每日 3次 ,对照组 5 9例 ,常规保肝治疗 ,3个月为 1疗程 ,1疗程结束后检测患者肝纤维化指标。结果 :软肝再生颗粒对慢性肝炎、肝硬变肝纤维化指标均有明显下降 ,慢性肝炎HA、LN、PCⅢ分别为 15 6 .1± 99.7ng/ml 117.9± 4 0 .6ng/ml 12 3.7± 38.6ng/ml,与对照组治疗后比较HA、LN P <0 .0 5 ,PCⅢ P <0 .0 1,肝硬变HA、LN、ⅣC、PCⅢ分别为 2 98.3± 94 .6ng/ml 139.3± 5 6 .9ng/ml 78.3± 2 9.4ng/ml 14 6 .1± 75 .2ng/ml,与对照组治疗后比较LN、ⅣC P <0 .0 5 ,HA、PCⅢ P <0 .0 1。结论

软肝再生颗粒治疗26例慢性肝炎肝纤维化的疗效观察

目的:观察软肝再生颗粒治疗慢性肝炎肝纤维化的疗效。方法:随机将50例慢性肝炎肝纤维化患者分为治疗组26例,对照组24例,治疗组给予软肝再生颗粒,每次12 g,3次/d;对照组给予护肝片,每次4片、3次/d,疗程均为3个月。观察治疗前后的临床症状、体征的变化、及肝脾影象学的变化,肝功能、肝纤维化等指标的变化和药物的不良反应。结果:软肝再生颗粒临床使用安全、可靠,在肝功能改善、症状缓解,有一定的效果;治疗组26例,与对照组24例比较,脾厚测量平均值明显缩小,肝纤维化指标HA和IV-C明显下降,LN无明显改变。结论:软肝再生颗粒治疗肝硬化肝纤维化临床应用安全、可靠,在保肝、抗肝纤维化方面具有一定的疗效。

意大利IMF树脂砂再生设备的结构、原理及特点

介绍意大利IMF公司的树脂砂再生设备 ,该设备采用塔式联合结构和震动软再生旧砂的方法 ,具有结构紧凑、全封闭、占地面积小、布线灵活等特点 ;再生能力强、再生砂品质好、没有粉尘污染 ;借鉴其经验 ,对国产树脂砂再生设备的开发、研制提出建议。

基于袋状可吸收生物膜行上颌窦瘘软、硬组织同期修补术的临床应用及操作规范

上颌窦瘘是由于多种因素造成的异常口腔-上颌窦交通,是口腔颌面外科较为常见的一种疾病。传统上对于上颌窦的修补为基于各种软组织瓣的转移修复,在修补上颌窦瘘的同时,缺少骨组织的同期修复。基于袋状可吸收生物膜技术,单纯利用生物医用材料,在不用自体骨的情况下可完成上颌窦瘘区域软、硬组织的同期修复与再生。该治疗技术具有创伤小、手术难度适中,临床效果可靠,易于推广等特点,越来越受到医师和患者的青睐。但由于口腔医师临床技术水平参差不齐,为规范该技术临床操作标准、保障治疗效果、降低术后并发症,在此提出上颌窦瘘软、硬组织同期修补术的临床应用及操作规范,旨在为广大口腔医师在应用该项技术时提供参考。

浓缩生长因子在上颌前牙区引导骨再生术中的临床应用

目的:评价浓缩生长因子(concentrated growth factors,CGF)在上颌前牙区引导骨再生术中的临床疗效。方法:选取上颌单个前牙缺失伴唇侧牙槽骨缺损患者63例,随机分为实验组(32例)和对照组(31例)。实验组植入CGF+Bio-Oss骨粉,表面覆盖CGF膜,对照组植入Bio-Oss骨粉,表面覆盖海奥生物膜。分别于术前、术后即刻和术后6个月拍摄锥形束CT(cone beam computed tomography,CBCT),并于术前、术后6个月测量术区角化龈宽度,比较两组手术前后CBCT和临床数据的变化。结果:实验组与对照组水平骨宽度术后较术前均有明显增加,差异有统计学意义(P<0.01),实验组术后6个月水平骨增量显著高于对照组(P<0.05),实验组的骨吸收率明显低于对照组,差异有统计学意义(P<0.05)。实验组术后6个月角化龈宽度较术前显著增加,差异有统计学意义(P<0.05),对照组无明显变化(P>0.05)。结论:CGF应用于上颌前牙引导骨再生术中表现出了较强的软硬组织再生能力,是一种较理想的骨增量技术。

软肝再生颗粒与恩替卡韦合用对乙肝患者肝纤维化的影响

目的观察软肝再生颗粒联合恩替卡韦治疗对慢性乙型肝炎血清肝纤维化指标的影响,评估此方案抗肝纤维化及肝功能复常的疗效。方法 148例患者随机分为治疗组76例,对照组72例;治疗组给予软肝再生颗粒12 g/包,3次/天,恩替卡韦0.5 mg/d,对照组给予恩替卡韦0.5 mg/d,疗程均为96周。于48周、96周观察两组患者的肝功能、肝纤维化指标、超声门静脉主干内径、脾脏厚度、Fibscan检测肝脏硬度值。结果治疗48周时,治疗组肝功能、肝纤维化指标、肝脏硬度值较治疗前相比差异有统计学意义(P<0.05),治疗96周时差异更明显(P<0.01)。治疗96周时,与对照组相比,肝功能恢复、肝纤维化指标、肝脏硬度值、门静脉主干内径、脾脏厚度下降值均优于对照组(P<0.05)。结论软肝再生颗粒联合恩替卡韦治疗可较好地改善慢性乙型肝炎肝纤维化指标,提示两药合用具有较好的抗纤维化效果。

后危机时代 大陆企业如何经营

历经2008年、2009年的世纪金融大风暴，我国的企业犹如大病初愈，不少企业元气大伤，身体虚弱。若遭到再次的风雨袭击，其后果将令人担忧。

富血小板纤维蛋白在口腔科的临床应用进展

富血小板纤维蛋白（Platelet-rich Fibrin，PRF）被誉为第二代血小板浓缩物，是自体血液经离心而获得，其分子结构类似天然血凝块，其内含有多种生长因子，可对组织修复过程进行调控及免疫调节，其纤维成分可起到三维生物支架作用，有利于新生组织的爬行替代及新生血管的长入，其在组织再生中发挥着重要的作用。本文就PRF应用于口腔软硬组织再生的临床应用进展做一综述。

软-硬复配温拌再生沥青混合料机理及性能

软-硬复配温拌再生沥青混合料是先采用高标号基质沥青对再生混合料预拌,后采用硬质天然沥青复拌而开发的温拌再生混合料。通过微观机理分析,揭示了沥青老化及软-硬复配再生机理;开展了不同RAP掺量温拌再生沥青混合料与70号热拌沥青混合料、SBS改性沥青混合料的高温性能、低温性能、水稳定性以及疲劳性能的对比研究。结果表明:软-硬复配温拌再生技术可行,110号基质沥青∶TLA湖沥青=7∶3温拌再生沥青混合料路用性能良好,20%RAP掺量为最佳;相比于70号热拌沥青混合料和SBS改性沥青混合料,施工温度可分别降低15℃、30℃～35℃。

Repair of articular cartilage defects in minipigs by microfracture surgery and BMSCs transplantation

Objective:To investigate the feasibility of minimal invasive repair of cartilage defect by arthroscope-aided microfracture surgery and autologous transplantation of mesenchymal stem cells. Methods: Bone marrow of minipigs was taken out and the bone marrow derived mesenchymal stem cells (BMSCs) were isolated and cultured to passage 3. Then 6 minipigs were randomly divided into 2 groups with 6 knees in each group. After the articular cartilage defect was induced in each knee, the left defect received microfracture surgery and was injected with 2.5 ml BMSCs cells at a concentration of 3×107 cells/ml into the articular cavity; while right knee got single microfracture or served as blank control group. The animals were killed at 8 or 16 weeks, and the repair tissue was histologically and immunohistochemically examined for the presence of type Ⅱ collagen and glycosaminoglycans (GAGs) at 8 and 16 weeks. Results: Eight weeks after the surgery, the overlying articular surface of the cartilage defect showed normal color and integrated to adjacent cartilage. And 16 weeks after surgery, hyaline cartilage was observed at the repairing tissues and immunostaining indicated the diffuse presence of this type Ⅱ collagen and GAGs throughout the repair cartilage in the treated defects. Single microfracture group had the repairing of fibrocartilage, while during the treatment, the defects of blank group were covered with fewer fiber tissues, and no blood capillary growth or any immunological rejection was observed. Conclusion: Microfracture technique and BMSCs transplantation to repair cartilage defect is characterized with minimal invasion and easy operation, and it will greatly promote the regeneration repair of articular cartilage defect.

Optimal Configuration for Distributed Generations in Micro-grid System Considering Diesel as the Main Control Source

This paper proposes an optimal configuration of the distributed hybrid renewable generations based on the stand-alone micro-grid system, considering the diesel as the main control source. Due to the natural sources and load of user changes randomly and the non-tinearity of the power output by renewable generations, an intelligent optimization method based on the improvement of the genetic algorithm and the control strategy are discussed. The instance analysis is compared with the optimization result of the hybrid system based on HOMER （hybrid optimization of multiple energy resources） and GA （genetic algorithm） method on Matlab software. The simulation result of the optimal configuration showed the new hybrid renewable system and would improve the power supply situation which decreased the cost of energy greatly compared with the conventional form of power supply system which was operated only by diesel. The conclusion of the comparing result between HOMER and GA method shows the advantages of the strategy for the diesel as main control sources.

Modeling and Simulation of Hydrogen Storage Device for Fuel Cell Plant

The article reviews a brief literature on the modeling of hydrogen storage device for fuel cell. Different dimensional approaches in modeling hydrogen absorption/desorption in a metal hydride reactor for use in fuel cell are summarized. Mathematical modeling equations involved are also stated. The effect of various operating parameters such as temperature, concentration, viscosity, thermal conductivity and time on the gas is also verified. The importance of various simulation software with reference to their major functions is also identified. The review concludes on the opportunities and challenges with the use of hydrogen as an alternative renewable energy.

Whole meniscus regeneration using polymer scaffolds oaded with fibrochondrocytes

Objective： To study the feasibility of regenerating a whole menisci using poly-（3- hydroxybutyrate-co-3-hydroxyvalerate） （PHBV） scaffolds loaded with meniscal cells in rabbits undergoing total meniscectomy, and to explore its protective effect on carti- lage degeneration. Methods： A solvent casting and particulate leaching technique was employed to fabricate biodegradable PHBV scaffolds into a meniscal shape. The proliferated meniscal cells were seeded onto the polymer scaffolds, transplanted into rabbit knee joints whose lateral menisci had been removed. Eight to 18 weeks after transplantation, the rege- nerated neomenisci were evaluated by gross and histologi- cal observations. Cartilage Mankin score. degeneration was assessed by Results： Eighteen weeks after transplantation, the implants formed neomenisci. Hematoxylin and eosin （HE） staining of the neomenisci sections revealed regeneration of fibrocartilage. Type I collagen in the neomenisci was also proved similar to normal meniscal tissue by immunohis-tochemical analysis and Sirius scarlet trinitrophenol staining. Articular cartilage degeneration was observed 8 weeks af- ter implantation. It was less severe as compared with that in total meniscectomy controls and no further degeneration was observed at 18 weeks. At that time, the regenerated neomenisci strongly resembled normal meniscal fibrocarti- lage in gross and histological appearance, and its mechani- cal property was also close to that of normal meniscus. Conclusions： The present study demonstrates the feasibility of tissue-engineering a whole meniscal structure in total meniscectomy rabbit models using biodegradable PHBV scaffolds together with cultured allogeneic meniscal cells. Cartilage degeneration is decreased. But long-term in vivo investigations on the histological structure and cartilage degeneration of the neomenisci regenerated by this method are still necessary to determine the clinical potential of this tissue engineering avenue.

4-Axis printing microfibrous tubular scaffold and tracheal cartilage application

Long-segment defects remain a major problem in clinical treatment of tubular tissue reconstruction.The design of tubular scaffold with desired structure and functional properties suitable for tubular tissue regeneration remains a great challenge in regenerative medicine.Here,we present a reliable method to rapidly fabricate tissueengineered tubular scaffold with hierarchical structure via 4-axis printing system.The fabrication process can be adapted to various biomaterials including hydrogels,thermoplastic materials and thermosetting materials.Using polycaprolactone(PCL)as an example,we successfully fabricated the scaffolds with tunable tubular architecture,controllable mesh structure,radial elasticity,good flexibility,and luminal patency.As a preliminary demonstration of the applications of this technology,we prepared a hybrid tubular scaffold via the combination of the 4-axis printed elastic poly(glycerol sebacate)(PGS)bio-spring and electrospun gelatin nanofibers.The scaffolds seeded with chondrocytes formed tubular mature cartilage-like tissue both via in vitro culture and subcutaneous implantation in the nude mouse,which showed great potential for tracheal cartilage reconstruction.