In multiloci-based genetic association studies of complex diseases, a powerful and high efficient tool for analyses oflinkage disequilibrium (LD) between markers, haplotype distributions and many chi-square/p values w...In multiloci-based genetic association studies of complex diseases, a powerful and high efficient tool for analyses oflinkage disequilibrium (LD) between markers, haplotype distributions and many chi-square/p values with a large numberof samples has been sought for long. In order to achieve the goal of obtaining meaningful results directly from raw data,we developed a robust and user-friendly software platform with a series of tools for analysis in association study withhigh efficiency. The platform has been well evaluated by several sets of real data.展开更多
Top structure and basement will confront the risk of being damaged on account of large stress and strain fields incurred by differential uplift and settlement between inner column and diaphragm wall in top-down method...Top structure and basement will confront the risk of being damaged on account of large stress and strain fields incurred by differential uplift and settlement between inner column and diaphragm wall in top-down method. Top-down excavation of the Metro Line 10 in Shanghai was modeled with finite element analysis software ABAQUS and parameters of subsoil were obtained by inverse analysis. Based on the finite element model and parameters, changes in the following factors were made to find more effective methods to restrain differential uplift and settlement: length of diaphragm wall, thickness of jet-grouting reinforcement layer, ways of subsoil reinforcement, sequence of pit excavation, connection between slabs and diaphragm wall or column and width of pit. Several significant results are acquired. The longer the diaphragm wall is, the greater the differential uplift between column and diaphragm wall is. Rigidity of roof slab is in general not strong enough to keep diaphragm wall and column undergoing the same uplift during excavation; Uplift at head of column and differential uplift between column and diaphragm wall decrease when subsoil from-16.6 to-43 m in pit is reinforced through jet-grouting. But, as excavation proceeds to a lower level, benefit from soil reinforcement diminishes. During the process applying vertical load, the larger the depth of diaphragm wall is, the smaller the settlement is at head of column and diaphragm wall, and the greater the differential settlement is between column and diaphragm wall. When friction connection is implemented between column, diaphragm wall and floor slabs, uplifts at head of column and diaphragm wall are larger than those of the case when tie connection is implemented, and so does differential uplift between column and diaphragm wall. The maximum deflection of diaphragm wall decreases by 58% on account of soil reinforcement in pit. The maximum deflection of diaphragm wall decreases by 61.2% when friction connection is implemented instead of tie connection.展开更多
Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, on...Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, only one deiodinase has been identified in the scallop C hlamys farreri. Here, two deiodinases were cloned in the Pacific oyster C rassostrea gigas( Cg Dx and C g Dy). The characteristic in-frame TGA codons and selenocysteine insertion sequence elements in the oyster deiodinase c DNAs supported the activity of them. Furthermore, seven orthologs of deiodinases were found by a tblastn search in the mollusk Lottia gigantea and the annelid C apitella teleta. A phylogenetic analysis revealed that the deiodinase gene originated from an common ancestor and a clade-specific gene duplication occurred independently during the differentiation of the mollusk, annelid, and vertebrate lineages. The distinct spatiotemporal expression patterns implied functional divergence of the two deiodinases. The expression of C g Dx and Cg Dy was influenced by L-thyroxine T4, and putative thyroid hormone responsive elements were found in their promoters, which suggested that the oyster deiodinases were feedback regulated by TH. Epinephrine stimulated the expression level of C g Dx and Cg Dy, suggesting an interaction effect between different hormones. This study provides the first evidence for the existence of a conserved TH feedback regulation mechanism in mollusks, providing insights into TH evolution.展开更多
Based on elastoplastic model, 2D and 3D finite element method （FEM） are used to calculate the stress and displacement distribution in the soft clay slope under gravity and uniform load at the slope top. Stability an...Based on elastoplastic model, 2D and 3D finite element method （FEM） are used to calculate the stress and displacement distribution in the soft clay slope under gravity and uniform load at the slope top. Stability analyses indicate that 3D boundary effect varies with the stress level of the slope. When the slope is stable, end effect of 3D space is not remarkable. When the stability decreases, end effect occurs; when the slope is at limit state, end effect reaches maximum. The energy causing slope failure spreads preferentially along y-z section, and when the failure resistance capability reaches the limit state, the energy can extend along x-axis direction. The 3D effect of the slope under uniform load on the top is related to the ratio of load influence width to slope height, and the effect is remarkable with the decrease of the ratio.展开更多
In the current theory of bridge foundation design,all of the loads above the cap are loaded by the pile,and the bearing capacity of the soil among piles is not taken into account.In order to analyze the bearing capaci...In the current theory of bridge foundation design,all of the loads above the cap are loaded by the pile,and the bearing capacity of the soil among piles is not taken into account.In order to analyze the bearing capacity of the soil among piles in bridge pile foundation,a model of pile foundation is established based on a bridge foundation which is under construction,and by the finite element analysis software ANSYS.According to the results of finite element analysis(FEA)and current bridge foundation design theory,a feasible composite pile foundation which can be applied in the design of bridge foundation,is recommended.Additionally,a number of modifications are made to the original design.It was confirmed that these modifications derived from numerical simulations can improve the performance of the foundation.展开更多
To obtain the vertical earth pressure on a soft foundation box culvert and investigate the interaction of the soil-culvert-foundation system, both a centrifugal model test and a numerical simulation were conducted and...To obtain the vertical earth pressure on a soft foundation box culvert and investigate the interaction of the soil-culvert-foundation system, both a centrifugal model test and a numerical simulation were conducted and the comparisons with the current methods to determine the load on a culvert were completed. The results of the model test and numerical analysis are in satisfactory agreement, which shows that the direction of the shear stress between the culvert and the adjacent embankment depends on the differential settlement between them. A vertical earth pressure concentration appears on the culvert with a rigid piles foundation because of a downward shear stress. The ratio of the load on a soft foundation culvert and the overburden pressure above the culvert raises first and then decreases as the backfill height increases. In order to reduce the load on a culvert, it is suggested to limit the stiffness difference of the foundations under the culvert and embankment and to use a light backfill over the culvert.展开更多
Objective： This study was to investigate whether PTEN mutations play a role in the carcinogenesis of soft tissue sarcomas （STS）. Methods： Polymerase chain reaction-single strand conformation polymorphism （PCR-SSC...Objective： This study was to investigate whether PTEN mutations play a role in the carcinogenesis of soft tissue sarcomas （STS）. Methods： Polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to amplify 4 exons of PTEN and to analyze the conformation polymorphism, then DNA sequencing methods was used to detect point mutation of PTEN gene four exons of abnormal single strand conformation in soft tissue sarcomas. Results： Two of 86 cases showed 130th condon G→A missense mutation in the exon 8 of PTEN gene, and this mutation made Arg to change to Gin in PTEN protein structure 334th condon A→T missense mutation in the exon 8 of PTEN gene, and this mutation made Asn to change to Lys in PI-EN protein structure. Conclusion： These data indicated the existence of PTEN mutation in soft tissue sarcomas, but PTEN gene mutation rate is very low. PTEN mutation may prays an less important role in the development and malignant transformation of soft tissue sarcomas.展开更多
Behavior-based malware analysis is an important technique for automatically analyzing and detecting malware, and it has received considerable attention from both academic and industrial communities. By considering how...Behavior-based malware analysis is an important technique for automatically analyzing and detecting malware, and it has received considerable attention from both academic and industrial communities. By considering how malware behaves, we can tackle the malware obfuscation problem, which cannot be processed by traditional static analysis approaches, and we can also derive the as-built behavior specifications and cover the entire behavior space of the malware samples. Although there have been several works focusing on malware behavior analysis, such research is far from mature, and no overviews have been put forward to date to investigate current developments and challenges. In this paper, we conduct a survey on malware behavior description and analysis considering three aspects： malware behavior description, behavior analysis methods, and visualization techniques. First, existing behavior data types and emerging techniques for malware behavior description are explored, especially the goals, prin- ciples, characteristics, and classifications of behavior analysis techniques proposed in the existing approaches. Second, the in- adequacies and challenges in malware behavior analysis are summarized from different perspectives. Finally, several possible directions are discussed for future research.展开更多
基金This work was supported by the Major State Basic Research Development program of Chinathe National High Technology Research and Development Program of China.
文摘In multiloci-based genetic association studies of complex diseases, a powerful and high efficient tool for analyses oflinkage disequilibrium (LD) between markers, haplotype distributions and many chi-square/p values with a large numberof samples has been sought for long. In order to achieve the goal of obtaining meaningful results directly from raw data,we developed a robust and user-friendly software platform with a series of tools for analysis in association study withhigh efficiency. The platform has been well evaluated by several sets of real data.
基金Projects(51208071,51108312) supported by the National Natural Science Foundation of China
文摘Top structure and basement will confront the risk of being damaged on account of large stress and strain fields incurred by differential uplift and settlement between inner column and diaphragm wall in top-down method. Top-down excavation of the Metro Line 10 in Shanghai was modeled with finite element analysis software ABAQUS and parameters of subsoil were obtained by inverse analysis. Based on the finite element model and parameters, changes in the following factors were made to find more effective methods to restrain differential uplift and settlement: length of diaphragm wall, thickness of jet-grouting reinforcement layer, ways of subsoil reinforcement, sequence of pit excavation, connection between slabs and diaphragm wall or column and width of pit. Several significant results are acquired. The longer the diaphragm wall is, the greater the differential uplift between column and diaphragm wall is. Rigidity of roof slab is in general not strong enough to keep diaphragm wall and column undergoing the same uplift during excavation; Uplift at head of column and differential uplift between column and diaphragm wall decrease when subsoil from-16.6 to-43 m in pit is reinforced through jet-grouting. But, as excavation proceeds to a lower level, benefit from soil reinforcement diminishes. During the process applying vertical load, the larger the depth of diaphragm wall is, the smaller the settlement is at head of column and diaphragm wall, and the greater the differential settlement is between column and diaphragm wall. When friction connection is implemented between column, diaphragm wall and floor slabs, uplifts at head of column and diaphragm wall are larger than those of the case when tie connection is implemented, and so does differential uplift between column and diaphragm wall. The maximum deflection of diaphragm wall decreases by 58% on account of soil reinforcement in pit. The maximum deflection of diaphragm wall decreases by 61.2% when friction connection is implemented instead of tie connection.
基金Supported by the National Natural Science Foundation of China(Nos.31372515,31402285)the National Basic Research Program of China(973 Program)(No.2010CB126401)the National High Technology Research and Development Program(863 Program)(No.2012AA10A405)
文摘Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, only one deiodinase has been identified in the scallop C hlamys farreri. Here, two deiodinases were cloned in the Pacific oyster C rassostrea gigas( Cg Dx and C g Dy). The characteristic in-frame TGA codons and selenocysteine insertion sequence elements in the oyster deiodinase c DNAs supported the activity of them. Furthermore, seven orthologs of deiodinases were found by a tblastn search in the mollusk Lottia gigantea and the annelid C apitella teleta. A phylogenetic analysis revealed that the deiodinase gene originated from an common ancestor and a clade-specific gene duplication occurred independently during the differentiation of the mollusk, annelid, and vertebrate lineages. The distinct spatiotemporal expression patterns implied functional divergence of the two deiodinases. The expression of C g Dx and Cg Dy was influenced by L-thyroxine T4, and putative thyroid hormone responsive elements were found in their promoters, which suggested that the oyster deiodinases were feedback regulated by TH. Epinephrine stimulated the expression level of C g Dx and Cg Dy, suggesting an interaction effect between different hormones. This study provides the first evidence for the existence of a conserved TH feedback regulation mechanism in mollusks, providing insights into TH evolution.
文摘Based on elastoplastic model, 2D and 3D finite element method （FEM） are used to calculate the stress and displacement distribution in the soft clay slope under gravity and uniform load at the slope top. Stability analyses indicate that 3D boundary effect varies with the stress level of the slope. When the slope is stable, end effect of 3D space is not remarkable. When the stability decreases, end effect occurs; when the slope is at limit state, end effect reaches maximum. The energy causing slope failure spreads preferentially along y-z section, and when the failure resistance capability reaches the limit state, the energy can extend along x-axis direction. The 3D effect of the slope under uniform load on the top is related to the ratio of load influence width to slope height, and the effect is remarkable with the decrease of the ratio.
文摘In the current theory of bridge foundation design,all of the loads above the cap are loaded by the pile,and the bearing capacity of the soil among piles is not taken into account.In order to analyze the bearing capacity of the soil among piles in bridge pile foundation,a model of pile foundation is established based on a bridge foundation which is under construction,and by the finite element analysis software ANSYS.According to the results of finite element analysis(FEA)and current bridge foundation design theory,a feasible composite pile foundation which can be applied in the design of bridge foundation,is recommended.Additionally,a number of modifications are made to the original design.It was confirmed that these modifications derived from numerical simulations can improve the performance of the foundation.
基金Project(2012AA112504) supported by the National High Technology Research and Development Program of ChinaProjects(51108048,51478054) supported by the National Natural Science Foundation of China
文摘To obtain the vertical earth pressure on a soft foundation box culvert and investigate the interaction of the soil-culvert-foundation system, both a centrifugal model test and a numerical simulation were conducted and the comparisons with the current methods to determine the load on a culvert were completed. The results of the model test and numerical analysis are in satisfactory agreement, which shows that the direction of the shear stress between the culvert and the adjacent embankment depends on the differential settlement between them. A vertical earth pressure concentration appears on the culvert with a rigid piles foundation because of a downward shear stress. The ratio of the load on a soft foundation culvert and the overburden pressure above the culvert raises first and then decreases as the backfill height increases. In order to reduce the load on a culvert, it is suggested to limit the stiffness difference of the foundations under the culvert and embankment and to use a light backfill over the culvert.
基金Supported by a grant from the National Natural Science Foundation of China (No. 30560169)
文摘Objective： This study was to investigate whether PTEN mutations play a role in the carcinogenesis of soft tissue sarcomas （STS）. Methods： Polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to amplify 4 exons of PTEN and to analyze the conformation polymorphism, then DNA sequencing methods was used to detect point mutation of PTEN gene four exons of abnormal single strand conformation in soft tissue sarcomas. Results： Two of 86 cases showed 130th condon G→A missense mutation in the exon 8 of PTEN gene, and this mutation made Arg to change to Gin in PTEN protein structure 334th condon A→T missense mutation in the exon 8 of PTEN gene, and this mutation made Asn to change to Lys in PI-EN protein structure. Conclusion： These data indicated the existence of PTEN mutation in soft tissue sarcomas, but PTEN gene mutation rate is very low. PTEN mutation may prays an less important role in the development and malignant transformation of soft tissue sarcomas.
基金Project supported by the National Natural Science Foundation of China(No.61472437)
文摘Behavior-based malware analysis is an important technique for automatically analyzing and detecting malware, and it has received considerable attention from both academic and industrial communities. By considering how malware behaves, we can tackle the malware obfuscation problem, which cannot be processed by traditional static analysis approaches, and we can also derive the as-built behavior specifications and cover the entire behavior space of the malware samples. Although there have been several works focusing on malware behavior analysis, such research is far from mature, and no overviews have been put forward to date to investigate current developments and challenges. In this paper, we conduct a survey on malware behavior description and analysis considering three aspects： malware behavior description, behavior analysis methods, and visualization techniques. First, existing behavior data types and emerging techniques for malware behavior description are explored, especially the goals, prin- ciples, characteristics, and classifications of behavior analysis techniques proposed in the existing approaches. Second, the in- adequacies and challenges in malware behavior analysis are summarized from different perspectives. Finally, several possible directions are discussed for future research.
