A cDNA library was successfully constructed from Anip973, a human lung adenocarcinoma cell line with high metastatic potential. NIH3T3 cells were stably transfected using this cDNA library and screened for morphologic...A cDNA library was successfully constructed from Anip973, a human lung adenocarcinoma cell line with high metastatic potential. NIH3T3 cells were stably transfected using this cDNA library and screened for morphological changes in a soft agar assay. Genomic DNA was isolated from putative clones and the integrated sequence was retrieved by PCR and sequencing. Three known genes, ribosomal protein L23, hypothetical protein FLJ22104, and serine protease inhibitor, kazal type 6 and a number of 5'-terminally truncated sequences were identified. Furthermore, cells transfected with ribosomal protein L23 was highly invasive compared with the empty vector as control (P 〈 0.02). These results indicate that the expression cloning of cDNA libraries in NIH3T3 cells and subsequent screening for loss of contact inhibition in soft agar is a viable tool for identifying tumor-related genes and ribosomal protein L23 gene plays a role in cell movement and metastasis.展开更多
Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing sect...Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing section.This system studied the 2charge-coupled device(CCD)camera(AD-080CL)of the JAI company.Fusion algorithm of visible light and near infrared images was designed for the fluorescence imaging system with wavelet transform image fusion algorithm.In order to enhance the fluorescent moiety of the fusion image,the luminance value of the green component of the color image was changed.And using microsoft foundation classes(MFC)application architecture,the supporting software system was bulit in VS2010 environment.展开更多
This paper presents a tool for managing, reusing and analysing C software code based on database techniques. The abstract information of entire software code is stored in a program database that is the conceptual sche...This paper presents a tool for managing, reusing and analysing C software code based on database techniques. The abstract information of entire software code is stored in a program database that is the conceptual scheme of the entire software, whereas the reuse component is a subscheme. Relational algebra can be conveniently used to manage, analyse and reuse C code. In the tool, we can manage, analyse and reuse any components in the program database and rapidly extract source code of any components or construct the program code of a new system. The rule system is introduced in reusing source code.展开更多
There exists an inherent difficulty in the original algorithm for the construction of Dwarf, which prevents it from constructing true Dwarfs. We explained when and why it introduces suffix redundancies into the Dwarf ...There exists an inherent difficulty in the original algorithm for the construction of Dwarf, which prevents it from constructing true Dwarfs. We explained when and why it introduces suffix redundancies into the Dwarf structure. To solve this problem, we proposed a completely new algorithm called PID. It bottom-up computes partitions of a fact table, and inserts them into the Dwarf structure. If a partition is an MSV partition, coalesce its sub-Dwarf; otherwise create necessary nodes and cells. Our performance study showed that PID is efficient. For further condensing of Dwarf, we proposed Condensed Dwarf, a more com- pressed structure, combining the strength of Dwarf and Condensed Cube. By eliminating unnecessary stores of “ALL” cells from the Dwarf structure, Condensed Dwarf could effectively reduce the size of Dwarf, especially for Dwarfs of the real world, which was illustrated by our experiments. Its query processing is still simple and, only two minor modifications to PID are required for the construction of Condensed Dwarf.展开更多
There has not been life cycle assessment (LCA) software applicable to China's products. To faciliate the development of LCA software system in China, we built an object-oriented LCA software system plantform based...There has not been life cycle assessment (LCA) software applicable to China's products. To faciliate the development of LCA software system in China, we built an object-oriented LCA software system plantform based on LCA international standards ISO 14040 to ISO 14043 and the commonness of diverse products. The system includes six modules: disassembly module, data collection module, arithmetic module, drawing module, database module and control module. It evaluates all economic and environmental impacts during the whole life cycle of a product. The integrated life cycle inventory database system allows dynamic expansion, which makes the effort required for data collection reduce with the increase of the system's application. With the improvement of human environmental-awareness, the demand of environment-friendly products is increasing, and ecological design of products has become a critical part in products development. Consummation of the LCA software system will provide a powerful tool for designing and developing Chinese ecological products.展开更多
To describe the dynamic process of flood routing intuitively and realistically when storm surge disaster occurs,a method for ArcGIS data and Google Earth(GE) data integration is proposed,which realizes the importing a...To describe the dynamic process of flood routing intuitively and realistically when storm surge disaster occurs,a method for ArcGIS data and Google Earth(GE) data integration is proposed,which realizes the importing and integrating of basic geographic information into GE. Based on SketchUp and AutoCAD software,threedimension(3D) visualization of seawall and other tidal defense structures is achieved. By employing Microsoft Foundation Class Library(MFC),the related system modules and storm surge flood routing dynamic visualization system are developed. Therefore,dynamic visualization of flood routing process and interactive query of submerged area and inundated depth are implemented. A practical application case study of Tianjin Binhai New Area provides decision-making support for coastal seawall planning and storm surge disaster prevention and reduction.展开更多
The Wind Among the Reeds, written from 1889 to 1939, is regarded as one of the most remarkable poetry collections of William Butler Yeats. It altogether includes 80 poems touching upon several themes such as love, rel...The Wind Among the Reeds, written from 1889 to 1939, is regarded as one of the most remarkable poetry collections of William Butler Yeats. It altogether includes 80 poems touching upon several themes such as love, religion, dignity, and life. Yeats is one of the most distinguished Irish poets throughout the world, whose works perfectly embody the incorporation of romanticism, modernism, and occultism. It is noteworthy that in some of his poems, animals are portrayed frequently or even taken as the title of a poem, such as bird, fish, swan and so on. Therefore, this essay attempts to study the meaning of animal images of this poetry anthology in terms of different writing phases of Yeats. Firstly, the author builds the corpus of The Wind Among the Reeds and employs corpus search software Ant Cone to check the number and distribution of the animal image. Next, the author focuses on certain prominent images and investigates them further by analyzing the concordance lines of them. Thirdly, according to the result of distribution information, the author also attaches importance to the phenomenon of image combination in the poetry and then explores its function and effect. To conclude, by exploring the animal image in The WindAmong the Reeds, a deeper understanding of the poetry and the writing style of the poet will be gained on another level. What is more, a more direct and objective data is provided through the method of corpus and its relevant software, thereupon a new research approach is introduced.展开更多
The Vision Earth System is a interactive system by employing B/S model.The system has the function of query display and mutually displays relevant geologic information,integrating image information of one outcrop and ...The Vision Earth System is a interactive system by employing B/S model.The system has the function of query display and mutually displays relevant geologic information,integrating image information of one outcrop and realizing 3D geologic visualization.In this system,the basis is effective store,transmitting,display and quick query of enormous images and their properties data.From Java technology,this essay researches the elementary realization of Vision Earth System by adopting store formality of enormous images database,quick display image of website and quick image storage method.展开更多
This paper analyzes current status of teachers' speaking test, applies presently mature C/S system structure and oilers a network way for teachers' speaking exam. We provide the whole process of design and developme...This paper analyzes current status of teachers' speaking test, applies presently mature C/S system structure and oilers a network way for teachers' speaking exam. We provide the whole process of design and development in online speaking exam, illustrate several functional designs including item bank construction, auto-generating examination paper and test paper analysis and discuss various network programming development technology during teachers' speaking network examination intensively. Directed by software engineering theory, the system is finally designed and realized through feasible research, requirement analysis, general design, detailed design, and tests with popular system development language and powerful database management.展开更多
For modeling and simulation of distillation process, there are lots of special purpose simulators along with their model libraries, such as Aspen Plus and HYSYS. However, the models in these tools lack of flexibility ...For modeling and simulation of distillation process, there are lots of special purpose simulators along with their model libraries, such as Aspen Plus and HYSYS. However, the models in these tools lack of flexibility and are not open to the end-user. Models developed in one tool can not be conveniently used in others because of the barriers among these simulators. In order to solve those problems, a flexible and extensible distillation system model library is constructed in this study, based on the Modelica and Modelica-supported platform MWorks, by the object-oriented technology and level progressive modeling strategy. It supports the reuse of knowledge on different granularities: physical phenomenon, unit model and system model. It is also an interface-friendly, accurate, fast PC-based and easily reusable simulation tool, which enables end-user to customize and extend the framework to add new functionality or adapt the simulation behavior as required. It also allows new models to be composed programmatically or graphically to form more complex models by invoking the existing components. A conventional air distillation column model is built and calculated using the library, and the results agree well with that simulated in Anen Plus.展开更多
This paper introduces the Scientific Electronic Library Online (SciELO) program and its three main objectives. Then the paper presents the methodology of open access to scientific information by the SciELO. At last, i...This paper introduces the Scientific Electronic Library Online (SciELO) program and its three main objectives. Then the paper presents the methodology of open access to scientific information by the SciELO. At last, it shows the perspectives of open access in developing countries.展开更多
Exceptionally preserved faunas have greatly improved our understanding of animal origin and evolution,and systematic investigations of Cambrian sediments around the world during the last two decades have led to a dram...Exceptionally preserved faunas have greatly improved our understanding of animal origin and evolution,and systematic investigations of Cambrian sediments around the world during the last two decades have led to a dramatic increase in the num-bers of Konservat-Lagerstatten[1].展开更多
基金This work was supported by Returning Scholars Fund of Heilongjiang Province (No. LC04C02) the Department of Education Overseas Researcher Fund of Heilongjiang Province (No. 1054HZ013).
文摘A cDNA library was successfully constructed from Anip973, a human lung adenocarcinoma cell line with high metastatic potential. NIH3T3 cells were stably transfected using this cDNA library and screened for morphological changes in a soft agar assay. Genomic DNA was isolated from putative clones and the integrated sequence was retrieved by PCR and sequencing. Three known genes, ribosomal protein L23, hypothetical protein FLJ22104, and serine protease inhibitor, kazal type 6 and a number of 5'-terminally truncated sequences were identified. Furthermore, cells transfected with ribosomal protein L23 was highly invasive compared with the empty vector as control (P 〈 0.02). These results indicate that the expression cloning of cDNA libraries in NIH3T3 cells and subsequent screening for loss of contact inhibition in soft agar is a viable tool for identifying tumor-related genes and ribosomal protein L23 gene plays a role in cell movement and metastasis.
基金National Natural Science Foundation of China(No.61171177)National Major Scientific Equipment Development Projects of China(No.2013YQ240803)+1 种基金Natural Science Foundation for Young Scientists of Shanxi Province(No.2012021011-1)Scientific and Technological Project in Shanxi Province(No.20140321010-02)
文摘Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing section.This system studied the 2charge-coupled device(CCD)camera(AD-080CL)of the JAI company.Fusion algorithm of visible light and near infrared images was designed for the fluorescence imaging system with wavelet transform image fusion algorithm.In order to enhance the fluorescent moiety of the fusion image,the luminance value of the green component of the color image was changed.And using microsoft foundation classes(MFC)application architecture,the supporting software system was bulit in VS2010 environment.
文摘This paper presents a tool for managing, reusing and analysing C software code based on database techniques. The abstract information of entire software code is stored in a program database that is the conceptual scheme of the entire software, whereas the reuse component is a subscheme. Relational algebra can be conveniently used to manage, analyse and reuse C code. In the tool, we can manage, analyse and reuse any components in the program database and rapidly extract source code of any components or construct the program code of a new system. The rule system is introduced in reusing source code.
基金Project (No. 20030487032) supported by the Specialized Research Fund for the Doctoral Program of Higher Education, China
文摘There exists an inherent difficulty in the original algorithm for the construction of Dwarf, which prevents it from constructing true Dwarfs. We explained when and why it introduces suffix redundancies into the Dwarf structure. To solve this problem, we proposed a completely new algorithm called PID. It bottom-up computes partitions of a fact table, and inserts them into the Dwarf structure. If a partition is an MSV partition, coalesce its sub-Dwarf; otherwise create necessary nodes and cells. Our performance study showed that PID is efficient. For further condensing of Dwarf, we proposed Condensed Dwarf, a more com- pressed structure, combining the strength of Dwarf and Condensed Cube. By eliminating unnecessary stores of “ALL” cells from the Dwarf structure, Condensed Dwarf could effectively reduce the size of Dwarf, especially for Dwarfs of the real world, which was illustrated by our experiments. Its query processing is still simple and, only two minor modifications to PID are required for the construction of Condensed Dwarf.
文摘There has not been life cycle assessment (LCA) software applicable to China's products. To faciliate the development of LCA software system in China, we built an object-oriented LCA software system plantform based on LCA international standards ISO 14040 to ISO 14043 and the commonness of diverse products. The system includes six modules: disassembly module, data collection module, arithmetic module, drawing module, database module and control module. It evaluates all economic and environmental impacts during the whole life cycle of a product. The integrated life cycle inventory database system allows dynamic expansion, which makes the effort required for data collection reduce with the increase of the system's application. With the improvement of human environmental-awareness, the demand of environment-friendly products is increasing, and ecological design of products has become a critical part in products development. Consummation of the LCA software system will provide a powerful tool for designing and developing Chinese ecological products.
基金State Programs of Science and Technology Development of China(No.2013CB035902)Foun-dation for Innovative Research Groups of National Natural Science Foundation of China(No.51021004)Na-tional Natural Science Foundation of China(No.51079096)
文摘To describe the dynamic process of flood routing intuitively and realistically when storm surge disaster occurs,a method for ArcGIS data and Google Earth(GE) data integration is proposed,which realizes the importing and integrating of basic geographic information into GE. Based on SketchUp and AutoCAD software,threedimension(3D) visualization of seawall and other tidal defense structures is achieved. By employing Microsoft Foundation Class Library(MFC),the related system modules and storm surge flood routing dynamic visualization system are developed. Therefore,dynamic visualization of flood routing process and interactive query of submerged area and inundated depth are implemented. A practical application case study of Tianjin Binhai New Area provides decision-making support for coastal seawall planning and storm surge disaster prevention and reduction.
文摘The Wind Among the Reeds, written from 1889 to 1939, is regarded as one of the most remarkable poetry collections of William Butler Yeats. It altogether includes 80 poems touching upon several themes such as love, religion, dignity, and life. Yeats is one of the most distinguished Irish poets throughout the world, whose works perfectly embody the incorporation of romanticism, modernism, and occultism. It is noteworthy that in some of his poems, animals are portrayed frequently or even taken as the title of a poem, such as bird, fish, swan and so on. Therefore, this essay attempts to study the meaning of animal images of this poetry anthology in terms of different writing phases of Yeats. Firstly, the author builds the corpus of The Wind Among the Reeds and employs corpus search software Ant Cone to check the number and distribution of the animal image. Next, the author focuses on certain prominent images and investigates them further by analyzing the concordance lines of them. Thirdly, according to the result of distribution information, the author also attaches importance to the phenomenon of image combination in the poetry and then explores its function and effect. To conclude, by exploring the animal image in The WindAmong the Reeds, a deeper understanding of the poetry and the writing style of the poet will be gained on another level. What is more, a more direct and objective data is provided through the method of corpus and its relevant software, thereupon a new research approach is introduced.
基金Supported by National Oil-gas Project : No XQ-2004-07
文摘The Vision Earth System is a interactive system by employing B/S model.The system has the function of query display and mutually displays relevant geologic information,integrating image information of one outcrop and realizing 3D geologic visualization.In this system,the basis is effective store,transmitting,display and quick query of enormous images and their properties data.From Java technology,this essay researches the elementary realization of Vision Earth System by adopting store formality of enormous images database,quick display image of website and quick image storage method.
文摘This paper analyzes current status of teachers' speaking test, applies presently mature C/S system structure and oilers a network way for teachers' speaking exam. We provide the whole process of design and development in online speaking exam, illustrate several functional designs including item bank construction, auto-generating examination paper and test paper analysis and discuss various network programming development technology during teachers' speaking network examination intensively. Directed by software engineering theory, the system is finally designed and realized through feasible research, requirement analysis, general design, detailed design, and tests with popular system development language and powerful database management.
基金Supported by the Major State Basic Research Development Program of China (2011CB706502)
文摘For modeling and simulation of distillation process, there are lots of special purpose simulators along with their model libraries, such as Aspen Plus and HYSYS. However, the models in these tools lack of flexibility and are not open to the end-user. Models developed in one tool can not be conveniently used in others because of the barriers among these simulators. In order to solve those problems, a flexible and extensible distillation system model library is constructed in this study, based on the Modelica and Modelica-supported platform MWorks, by the object-oriented technology and level progressive modeling strategy. It supports the reuse of knowledge on different granularities: physical phenomenon, unit model and system model. It is also an interface-friendly, accurate, fast PC-based and easily reusable simulation tool, which enables end-user to customize and extend the framework to add new functionality or adapt the simulation behavior as required. It also allows new models to be composed programmatically or graphically to form more complex models by invoking the existing components. A conventional air distillation column model is built and calculated using the library, and the results agree well with that simulated in Anen Plus.
文摘This paper introduces the Scientific Electronic Library Online (SciELO) program and its three main objectives. Then the paper presents the methodology of open access to scientific information by the SciELO. At last, it shows the perspectives of open access in developing countries.
基金the National Natural Science Foundation of China(41720104002,41890844,41425008 and 41621003)the Strategic Priority Research Program of Chinese Academy of Sciences(XDB26000000)+2 种基金the Program of Introducing Talents of Discipline to Universities(D17013)Research of L.E.Holmer supported by the Swedish Research Council(VR Project No.2018-03390)a Zhongjian Yang Scholarship from the Department of Geology,Northwest University,China。
文摘Exceptionally preserved faunas have greatly improved our understanding of animal origin and evolution,and systematic investigations of Cambrian sediments around the world during the last two decades have led to a dramatic increase in the num-bers of Konservat-Lagerstatten[1].