Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing sect...Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing section.This system studied the 2charge-coupled device(CCD)camera(AD-080CL)of the JAI company.Fusion algorithm of visible light and near infrared images was designed for the fluorescence imaging system with wavelet transform image fusion algorithm.In order to enhance the fluorescent moiety of the fusion image,the luminance value of the green component of the color image was changed.And using microsoft foundation classes(MFC)application architecture,the supporting software system was bulit in VS2010 environment.展开更多
OBJECTIVE: To assess the feasibility of detecting SYT-SSX fusion transcripts in paraffin-embedded tissues of synovial sarcoma by reverse transcription-polymerase chain reaction (RT-PCR). METHODS: RT-PCR was used to am...OBJECTIVE: To assess the feasibility of detecting SYT-SSX fusion transcripts in paraffin-embedded tissues of synovial sarcoma by reverse transcription-polymerase chain reaction (RT-PCR). METHODS: RT-PCR was used to amplify the SYT-SSX fusion transcripts using archival formalin-fixed paraffin-embedded tumor specimens from a series of 37 synovial sarcoma cases. To investigate the specificity of the SYT-SSX fusion transcripts, a variety of non-synovial sarcoma tumors were included in the study as negative controls. The detected messages derived from fusion genes were confirmed by subsequent sequence analysis. RESULTS: SYT-SSX fusion transcripts were detected in 33 of 37 (89.2%) synovial sarcomas. None of the 34 cases of non-synovial sarcoma tumors showed amplified products of SYT-SSX fusion transcripts, although PBGD mRNA was detected in all specimens. Among 33 SYT-SSX-positive synovial sarcomas, 22 tumors had an SYT-SSX 1 fusion transcript, whereas 6 tumors had an SYT-SSX2 fusion transcript. Fusion types can not be distinguished in the remaining 5 cases. There was a significant relationship between SYT-SSX fusion type and histologic subtype. All 10 biphasic synovial sarcomas had the SYT-SSX1 fusion, whereas all tumors with SYT-SSX2 were of monophasic morphology (P展开更多
This paper reports on the preparation of Fe82.7-85.7Si2-4.9B9.2-11.2P1.5.2.7C0,8 soft magnetic amorphous alloys with a distinctly high Fe content of 93.5-95.5 wt.% by component design and composition adjustment. All a...This paper reports on the preparation of Fe82.7-85.7Si2-4.9B9.2-11.2P1.5.2.7C0,8 soft magnetic amorphous alloys with a distinctly high Fe content of 93.5-95.5 wt.% by component design and composition adjustment. All alloys can be readily fabricated into completely amorphous ribbon samples with good surface quality by the single copper roller melt-spinning method. These alloys show good bending ductility and excellent magnetic properties after annealing, i.e., low coercivity （He） of 3.3-5.9 A/m, high permeability （μe） of 5000-10000 and high flux saturation density （Bs） of 1.63-1.66 T. The mechanism of the good glass forming ability （GFA）and soft-magnetic properties are explored. The amorphous alloys with the high Fe content comparable to that of the desired high Si alloy can be promising candidates for the potential application in electric devices.展开更多
基金National Natural Science Foundation of China(No.61171177)National Major Scientific Equipment Development Projects of China(No.2013YQ240803)+1 种基金Natural Science Foundation for Young Scientists of Shanxi Province(No.2012021011-1)Scientific and Technological Project in Shanxi Province(No.20140321010-02)
文摘Infrared and visible light images can be obtained simultaneously by building fluorescence imaging system,which includes fluorescence excitation,images acquisition,mechanical part,image transmission and processing section.This system studied the 2charge-coupled device(CCD)camera(AD-080CL)of the JAI company.Fusion algorithm of visible light and near infrared images was designed for the fluorescence imaging system with wavelet transform image fusion algorithm.In order to enhance the fluorescent moiety of the fusion image,the luminance value of the green component of the color image was changed.And using microsoft foundation classes(MFC)application architecture,the supporting software system was bulit in VS2010 environment.
文摘OBJECTIVE: To assess the feasibility of detecting SYT-SSX fusion transcripts in paraffin-embedded tissues of synovial sarcoma by reverse transcription-polymerase chain reaction (RT-PCR). METHODS: RT-PCR was used to amplify the SYT-SSX fusion transcripts using archival formalin-fixed paraffin-embedded tumor specimens from a series of 37 synovial sarcoma cases. To investigate the specificity of the SYT-SSX fusion transcripts, a variety of non-synovial sarcoma tumors were included in the study as negative controls. The detected messages derived from fusion genes were confirmed by subsequent sequence analysis. RESULTS: SYT-SSX fusion transcripts were detected in 33 of 37 (89.2%) synovial sarcomas. None of the 34 cases of non-synovial sarcoma tumors showed amplified products of SYT-SSX fusion transcripts, although PBGD mRNA was detected in all specimens. Among 33 SYT-SSX-positive synovial sarcomas, 22 tumors had an SYT-SSX 1 fusion transcript, whereas 6 tumors had an SYT-SSX2 fusion transcript. Fusion types can not be distinguished in the remaining 5 cases. There was a significant relationship between SYT-SSX fusion type and histologic subtype. All 10 biphasic synovial sarcomas had the SYT-SSX1 fusion, whereas all tumors with SYT-SSX2 were of monophasic morphology (P
基金the National Natural Science Foundation of China(Grant No.51541106)Ningbo International Cooperation Projects(Grant No.2015D10022)+2 种基金Ningbo Major Project for Science and Technology(Grant No.201401B1003003)Ningbo Natural Science Foundations(Grant No.2015A610007)General Research Fund of Hong Kong(Grant No.CityU 102013)
文摘This paper reports on the preparation of Fe82.7-85.7Si2-4.9B9.2-11.2P1.5.2.7C0,8 soft magnetic amorphous alloys with a distinctly high Fe content of 93.5-95.5 wt.% by component design and composition adjustment. All alloys can be readily fabricated into completely amorphous ribbon samples with good surface quality by the single copper roller melt-spinning method. These alloys show good bending ductility and excellent magnetic properties after annealing, i.e., low coercivity （He） of 3.3-5.9 A/m, high permeability （μe） of 5000-10000 and high flux saturation density （Bs） of 1.63-1.66 T. The mechanism of the good glass forming ability （GFA）and soft-magnetic properties are explored. The amorphous alloys with the high Fe content comparable to that of the desired high Si alloy can be promising candidates for the potential application in electric devices.
