Objective To observe the influence of acupuncture on hypothalamus-pituitary-ovary axis （HPOA） in rats with primary dysmenorrhea, and to explore its mechanisms. Methods Effects of acupuncture on the concentration lev...Objective To observe the influence of acupuncture on hypothalamus-pituitary-ovary axis （HPOA） in rats with primary dysmenorrhea, and to explore its mechanisms. Methods Effects of acupuncture on the concentration level of β-endorphin （β-EP）, hypothalamic hormone （gonadotropin-releasing hormone, GnRH）, pituitary hormone （GnRH receptor GnRH-R, follicle stimulating hormone FSH, luteinizing hormone LH） and ovarian hormone （estrodiol E2, estrodiol receptor ER, progesterone P and progesterone receptor PR） were observed in dysmenorrheic rats. Results After receiving acupuncture treatment, the levels of 13- EP, GnRH, GnRH-R, FSH, LH, E2, ER, P and PR were changed, and this suggested that acupuncture had a regulative action on HPOA in dysmenorrheic rats. Conclusion The mechanism of acupuncture acting on the HPOA with primary dysmenorrhea might be its regulating role to the gonadal hormone of HPOA and its corresponding receptor expression.展开更多
We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed...We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed dormancy.The seeds of P.anomala subsp.veitchii were treated with different concentrations of GA_(3)(0,100,200,300,and 400 mg/L)under dark conditions at 16℃,and then the rooting indicators including rooting rate,rooting vigor,rooting index and mold rot rate were determined.The treatment with 400 mg/L GA_(3) for 4 h showed the best performance in breaking the hypocotyl dormancy.Specifically,the treatment shortened the rooting time to 12 d,increased the rooting rate,rooting vigor,and rooting index to 84.00%±5.29%,80.67%±5.03%,and 2.25±0.16 respectively,and decreased the mold rot rate to 0.67%±1.16%.To sum up,there were germination inhibitors in the seeds of P.anomala subsp.veitchii,while the addition of exogenous hormone GA_(3) can release the inhibitory effect on the hypocotyl,which indicated that GA_(3) can promote the breaking of hypocotyl dormancy.展开更多
The heterotrimeric guanine nucleotide-binding protein （G-protein） has been demonstrated to mediate various signaling pathways in plants. However, its role in phytochrome A （phyA） signaling remains elusive. In this...The heterotrimeric guanine nucleotide-binding protein （G-protein） has been demonstrated to mediate various signaling pathways in plants. However, its role in phytochrome A （phyA） signaling remains elusive. In this study, we discover a new phyA-mediated phenotype designated far-red irradiation （FR） preconditioned cell death, which occurs only in the hypocotyls of FR-grown seedlings following exposure to white light （WL）. The cell death is mitigated in the Gα mutant gpal but aggravated in the Gβ mutant agbl in comparison with the wild type （WT）, indicative of antagonistic roles of GPA1 and AGBI in the phyA-mediated cell-death pathway. Further investigation indicates that FR-induced accumulation of nonphotoconvertible protochlorophyllide （Pchlide^633）, which generates reactive oxygen species （ROS） on exposure to WL, is required for FR-preconditioned cell death. Moreover, ROS is mainly detected in chloroplasts using the fluorescent probe. Interestingly, the application of H2O2 to dark-grown seedlings results in a phenotype similar to FR-preconditioned cell death. This reveals that ROS is a critical mediator for the ceil death. In addition, we observe that agb1 is more sensitive to H2O2 than WT seedlings, indicating that the G-protein may also modify the sensitivity of the seedlings to ROS stress. Taking these results together, we infer that the G-protein may be involved in the phyA signaling pathway to regulate FR-preconditioned cell death ofArabidopsis hypocotyls. A possible mechanism underlying the involvement of the G-protein in phyA signaling is discussed in this study.展开更多
基金National Basic Research Program of China(973 program,No:2006CB504502)Chinese Na-tional Foundation of Natural Sciences.(No.90209009)
文摘Objective To observe the influence of acupuncture on hypothalamus-pituitary-ovary axis （HPOA） in rats with primary dysmenorrhea, and to explore its mechanisms. Methods Effects of acupuncture on the concentration level of β-endorphin （β-EP）, hypothalamic hormone （gonadotropin-releasing hormone, GnRH）, pituitary hormone （GnRH receptor GnRH-R, follicle stimulating hormone FSH, luteinizing hormone LH） and ovarian hormone （estrodiol E2, estrodiol receptor ER, progesterone P and progesterone receptor PR） were observed in dysmenorrheic rats. Results After receiving acupuncture treatment, the levels of 13- EP, GnRH, GnRH-R, FSH, LH, E2, ER, P and PR were changed, and this suggested that acupuncture had a regulative action on HPOA in dysmenorrheic rats. Conclusion The mechanism of acupuncture acting on the HPOA with primary dysmenorrhea might be its regulating role to the gonadal hormone of HPOA and its corresponding receptor expression.
文摘We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed dormancy.The seeds of P.anomala subsp.veitchii were treated with different concentrations of GA_(3)(0,100,200,300,and 400 mg/L)under dark conditions at 16℃,and then the rooting indicators including rooting rate,rooting vigor,rooting index and mold rot rate were determined.The treatment with 400 mg/L GA_(3) for 4 h showed the best performance in breaking the hypocotyl dormancy.Specifically,the treatment shortened the rooting time to 12 d,increased the rooting rate,rooting vigor,and rooting index to 84.00%±5.29%,80.67%±5.03%,and 2.25±0.16 respectively,and decreased the mold rot rate to 0.67%±1.16%.To sum up,there were germination inhibitors in the seeds of P.anomala subsp.veitchii,while the addition of exogenous hormone GA_(3) can release the inhibitory effect on the hypocotyl,which indicated that GA_(3) can promote the breaking of hypocotyl dormancy.
文摘The heterotrimeric guanine nucleotide-binding protein （G-protein） has been demonstrated to mediate various signaling pathways in plants. However, its role in phytochrome A （phyA） signaling remains elusive. In this study, we discover a new phyA-mediated phenotype designated far-red irradiation （FR） preconditioned cell death, which occurs only in the hypocotyls of FR-grown seedlings following exposure to white light （WL）. The cell death is mitigated in the Gα mutant gpal but aggravated in the Gβ mutant agbl in comparison with the wild type （WT）, indicative of antagonistic roles of GPA1 and AGBI in the phyA-mediated cell-death pathway. Further investigation indicates that FR-induced accumulation of nonphotoconvertible protochlorophyllide （Pchlide^633）, which generates reactive oxygen species （ROS） on exposure to WL, is required for FR-preconditioned cell death. Moreover, ROS is mainly detected in chloroplasts using the fluorescent probe. Interestingly, the application of H2O2 to dark-grown seedlings results in a phenotype similar to FR-preconditioned cell death. This reveals that ROS is a critical mediator for the ceil death. In addition, we observe that agb1 is more sensitive to H2O2 than WT seedlings, indicating that the G-protein may also modify the sensitivity of the seedlings to ROS stress. Taking these results together, we infer that the G-protein may be involved in the phyA signaling pathway to regulate FR-preconditioned cell death ofArabidopsis hypocotyls. A possible mechanism underlying the involvement of the G-protein in phyA signaling is discussed in this study.
