As members of a super gene family, WRKY transcription factors are widely distributed in higher plants. ln this study, bioinformatic analysis of WRKY35, a member of the WRKY gene family, was carried out. Results indica...As members of a super gene family, WRKY transcription factors are widely distributed in higher plants. ln this study, bioinformatic analysis of WRKY35, a member of the WRKY gene family, was carried out. Results indicated that tran-scription factor WRKY35 harbors a WRKYGQK core domain and a Cys2His2 or Cys2His/Cys zinc finger in the 5’ end without transmembrane domain. After PCR amplification and restriction digestion, WRKY35 gene fragment was ligated to prokaryotic expression vector PET28. This study provided basis for expression anal-ysis of WRKY35 protein and subsequent functional identification of WRKY35 gene.展开更多
Fe3O4 magnetic nanoparticles were prepared by the aqueous co-precipitation of FeCl3-6H2O and FeCl2-4H2O with addition of ammonium hydroxide. The conditions for the preparation of Fe3O4 magnetic nanoparticles were opti...Fe3O4 magnetic nanoparticles were prepared by the aqueous co-precipitation of FeCl3-6H2O and FeCl2-4H2O with addition of ammonium hydroxide. The conditions for the preparation of Fe3O4 magnetic nanoparticles were optimized, and Fe3O4 magnetic nanoparticles obtained were characterized systematically by means of transmission electron microscope （TEM）, dynamic laser scattering analyzer （DLS） and X-ray diffraction （XRD）. The results revealed that the magnetic nanoparticles were cubic shaped and dispersive, with narrow size distribution and average diameter of 11.4 nm. It was found that the homogeneous variation of pH value in the solution via the control on the dropping rate of aqueous ammonia played a critical role in size distribution. The magnetic response of the product in the magnetic field was also analyzed and evaluated carefully. A 32.6 mT magnetic field which is produced by four ferromagnets was found to be sufficient to excite the dipole moments of 0.05 g Fe3O4 powder 2 cm far away from the ferromagnets. In conclusion, the Fe3O4 magnetic nanoparticles with excellent properties were competent for the magnetic carders of targeted-drug in future application.展开更多
By analyzing the correlation between modal calculations and modal experiments of a typical supporter, an effective finite element analysis( FEA)model of the actual aerospace supporter is created. According to the anal...By analyzing the correlation between modal calculations and modal experiments of a typical supporter, an effective finite element analysis( FEA)model of the actual aerospace supporter is created. According to the analysis of constrained viscoelastic damping, the strategies of PVC have been worked out, and the correlation between modal calculations and modal experiments of the supporter has also been computed, and then, an experiment has been designed based on the calculation results. The results of experiments verify that the PVC strategy can effectively suppress vibration.展开更多
In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad(Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction(PCR),and ob...In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad(Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction(PCR),and obtained a transcript of 1 075 bp consisting of 1 37 bp 5′ untranslated region(UTR),515 bp 3′ UTR and a 423 bp open reading frame(ORF) encoding a polypeptide of 140 amino acid residues(GenBank accession number: KF359945).Homolog analysis showed a 70%–96% homology with sterol carrier protein-2(SCP-2) present in other animals,which is implicated in lipid metabolism of other organisms.The gene SCP-2 of Chinese toad(B.gargarizans) was cloned from a first strand cDNA of Bufo skin(GenBank accession number: KF381341) via PCR,whose encoding polypeptide has only one amino acid difference from that of Japanese toad.Tissue distribution analysis showed that SCP-2 expressed in all organs tested,though in the liver and spleen it manifested lower expression than in other organs.These findings might indicate SCP-2 being one of the active ingredients in toad skin.These findings may in turn have implications for further drug development from traditional Chinese medicine sources.展开更多
VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability...VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore, anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.展开更多
Currently,the WLAN indoor positioning system attracts a lot of interests,not only because of the cheap implementation but also because of the high positioning accuracy comparing with other indoor positioning systems.T...Currently,the WLAN indoor positioning system attracts a lot of interests,not only because of the cheap implementation but also because of the high positioning accuracy comparing with other indoor positioning systems.The WLAN indoor positioning system contains two phases,which are offline phase and online phase.In the online phase,the WLAN equipment user(UE) has to access to the WLAN for the latest radio map and positioning software.Due to during the network allocation vector(NAV) duration,the WLAN channel is only reserved for one WLAN UE,others UEs' carrier accessing will be blocked.In addition,the blocked UE will make a retrial accessing,which will definitely introduce more traffic blocking to the WLAN.So In this paper,based on the analysis of the WLAN indoor positioning system architecture,a proper queuing model by using of the Extended Erlang B formula is proposed,which takes the retrial calling percentage into consideration in the proposed model.The simulation results show that the proposed method is more accurate and performs well to predict the blocking probability.展开更多
AIM:To investigate the suppressive activity of MUTYH variant proteins against mutations caused by oxidative lesion,8-hydroxyguanine(8OHG),in human cells.METHODS:p.R154H,p.M255V,p.L360P,and p.P377L MUTYH variants,which...AIM:To investigate the suppressive activity of MUTYH variant proteins against mutations caused by oxidative lesion,8-hydroxyguanine(8OHG),in human cells.METHODS:p.R154H,p.M255V,p.L360P,and p.P377L MUTYH variants,which were previously found in patients with colorectal polyposis and cancer,were selected for use in this study.Human H1299 cancer cell lines inducibly expressing wild-type(WT) MUTYH(type 2) or one of the 4 above-mentioned MUTYH variants were established using the piggyBac transposon vector system,enabling the genomic integration of the transposon sequence for MUTYH expression.MUTYH expression was examined after cumate induction using Western blotting analysis and immunofluorescence analysis.The intracellular localization of MUTYH variants tagged with FLAG was also immunofluorescently examined.Next,the mutation frequency in the supF of the shuttle plasmid pMY189 containing a single 8OHG residue at position 159 of the supF was compared between empty vector cells and cells expressing WT MUTYH or one of the 4 MUTYH variants using a supF forward mutation assay.RESULTS:The successful establishment of human cell lines inducibly expressing WT MUTYH or one of the 4 MUTYH variants was concluded based on the detection of MUTYH expression in these cell lines after treatment with cumate.All of the MUTYH variants and WT MUTYH were localized in the nucleus,and nuclear localization was also observed for FLAG-tagged MUTYH.The mutation frequency of supF was 2.2 × 10-2 in the 8OHG-containing pMY189 plasmid and 2.5 × 10-4 in WT pMY189 in empty vector cells,which was an 86-fold increase with the introduction of 8OHG.The mutation frequency(4.7 × 10-3) of supF in the 8OHG-containing pMY189 plasmid in cells overexpressing WT MUTYH was significantly lower than in the empty vector cells(P < 0.01).However,the mutation frequencies of the supF in the 8OHG-containing pMY189 plasmid in cells overexpressing the p.R154H,p.M255V,p.L360P,or p.P377L MUTYH variant were 1.84 × 10-2,1.55 × 10-2,1.91 × 10-2,and 1.96 × 10-2,respectively,meaning that no significant difference was observed in the mutation frequency between the empty vector cells and cells overexpressing MUTYH mutants.CONCLUSION:The suppressive activities of p.R154H,p.M255V,p.L360P,and p.P377L MUTYH variants against mutations caused by 8OHG are thought to be severely impaired in human cells.展开更多
Stress analysis of cylindrical grid-stiffened composite shells was conducted under transverse loading,pure bending,torsion and axial compression under clamped-free boundary condition.Electrical strain gauges were empl...Stress analysis of cylindrical grid-stiffened composite shells was conducted under transverse loading,pure bending,torsion and axial compression under clamped-free boundary condition.Electrical strain gauges were employed to measure the strains in transverse loading case to validate the finite element analysis which was conducted using ANSYS software.Good agreement was obtained between the two methods.It was observed that stiffening the composite shell with helical ribs decreased the average equivalent Von Mises stress on the shell.The reduction of the stress seemed to be higher in the intersection of two ribs.It was also seen that the stress reduction ratio was higher when the structure was under bending compared to torsion and axial compression.The reduction ratio was approximately 75% in pure bending in the intersection point of the ribs,while it was approximately 25% in torsion.Therefore,it is concluded that the presence of the ribs is more effective under bending.Failure analysis was done using Tsai-Wu criterion.The ribs were observed to result in maximum and minimum increase in the failure load of the structure under transverse bending and torsional loading,respectively.展开更多
The first decision we need to make in a structural load assessment is what approach should be applied, a linear approach or a non-linear one. The correct decision comes from understanding of the technology used in the...The first decision we need to make in a structural load assessment is what approach should be applied, a linear approach or a non-linear one. The correct decision comes from understanding of the technology used in the linear and non-linear approaches and also comes from the understanding of the problem to he analyzed. From engineering practice, it has been found that many non-linear effects can be taken into account in a linear model with appropriate approach. A study of hydrodynamic structural load on a stinger of a pipe-laying vessel is presented in this paper. The results of a non-linear analysis are compared to those of linear models with different approaches, and how the nonlinear effect can be involved in a linear model is discussed. The recommendations on how to estimate the non-linear effects in a linear structural load model is discussed.展开更多
Porous polymer supported palladium catalyst for cross coupling reactions with high activity has been successfully prepared by coordination of Pd 2+ species with Schiff bases functionalized porous polymer. The catalyst...Porous polymer supported palladium catalyst for cross coupling reactions with high activity has been successfully prepared by coordination of Pd 2+ species with Schiff bases functionalized porous polymer. The catalyst has been systemically investi-gated by a series of characterizations such as TEM, N 2 adsorption, NMR, IR, XPS, etc. TEM and N 2 isotherms show that the sample maintains the nanoporous structure after the modification and coordination. XPS results show that chemical state of palladium species in the catalyst is mainly +2. More importantly, the catalyst shows very high activities and excellent recycla-bility in a series of coupling reactions including Suzuki, Sonogashira, and Heck reactions. Hot filtration and poison of catalysts experiments have also been performed and the results indicate that soluble active species (mainly Pd(0) species) in-situ gener-ated from the catalyst under the reaction conditions are the active intermediates, which would redeposit to the supporter after the reactions.展开更多
基金Supported by National Natural Science Foundation of China "Functional analysis of transcription factor AtWRKY28 in development and morphogenesis of Orychophragmus violaceus"(31360262)Key Agricultural Science and Technology Project of Department of Science and Technology of Guizhou Province "Molecular Marker Development and Assisted Breeding of Recessive Epistatic Genic Male Sterile Lines of Rapeseed"(QKHNYZ[2012]3033)Special Fund of Guizhou Academy of Agricultural Sciences "BnaC.Tic40 (tic40) and BnRf (rf) Marker-assisted Breeding of Recessive Genic Male Sterile Three Lines of Rapeseed"(QNKYYZX[2012]002)~~
文摘As members of a super gene family, WRKY transcription factors are widely distributed in higher plants. ln this study, bioinformatic analysis of WRKY35, a member of the WRKY gene family, was carried out. Results indicated that tran-scription factor WRKY35 harbors a WRKYGQK core domain and a Cys2His2 or Cys2His/Cys zinc finger in the 5’ end without transmembrane domain. After PCR amplification and restriction digestion, WRKY35 gene fragment was ligated to prokaryotic expression vector PET28. This study provided basis for expression anal-ysis of WRKY35 protein and subsequent functional identification of WRKY35 gene.
基金the Technology Project of Jiangxi Provincial Education DepartmentJiangxi Provincial Science Department
文摘Fe3O4 magnetic nanoparticles were prepared by the aqueous co-precipitation of FeCl3-6H2O and FeCl2-4H2O with addition of ammonium hydroxide. The conditions for the preparation of Fe3O4 magnetic nanoparticles were optimized, and Fe3O4 magnetic nanoparticles obtained were characterized systematically by means of transmission electron microscope （TEM）, dynamic laser scattering analyzer （DLS） and X-ray diffraction （XRD）. The results revealed that the magnetic nanoparticles were cubic shaped and dispersive, with narrow size distribution and average diameter of 11.4 nm. It was found that the homogeneous variation of pH value in the solution via the control on the dropping rate of aqueous ammonia played a critical role in size distribution. The magnetic response of the product in the magnetic field was also analyzed and evaluated carefully. A 32.6 mT magnetic field which is produced by four ferromagnets was found to be sufficient to excite the dipole moments of 0.05 g Fe3O4 powder 2 cm far away from the ferromagnets. In conclusion, the Fe3O4 magnetic nanoparticles with excellent properties were competent for the magnetic carders of targeted-drug in future application.
基金Sponsored by the National 863 Project of China (Grant No. 863 -2 -416)
文摘By analyzing the correlation between modal calculations and modal experiments of a typical supporter, an effective finite element analysis( FEA)model of the actual aerospace supporter is created. According to the analysis of constrained viscoelastic damping, the strategies of PVC have been worked out, and the correlation between modal calculations and modal experiments of the supporter has also been computed, and then, an experiment has been designed based on the calculation results. The results of experiments verify that the PVC strategy can effectively suppress vibration.
基金Foundation items： This study was supported by the National Natural Science Foundation of China （31071181, 31372149） and the Students＇ Innovative Training Program of ZAFU （20120207, 20120213）
文摘In this study,to clarify the bioactive polypeptides included in the skins and secretions of Bufo,we screened the Japanese toad(Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction(PCR),and obtained a transcript of 1 075 bp consisting of 1 37 bp 5′ untranslated region(UTR),515 bp 3′ UTR and a 423 bp open reading frame(ORF) encoding a polypeptide of 140 amino acid residues(GenBank accession number: KF359945).Homolog analysis showed a 70%–96% homology with sterol carrier protein-2(SCP-2) present in other animals,which is implicated in lipid metabolism of other organisms.The gene SCP-2 of Chinese toad(B.gargarizans) was cloned from a first strand cDNA of Bufo skin(GenBank accession number: KF381341) via PCR,whose encoding polypeptide has only one amino acid difference from that of Japanese toad.Tissue distribution analysis showed that SCP-2 expressed in all organs tested,though in the liver and spleen it manifested lower expression than in other organs.These findings might indicate SCP-2 being one of the active ingredients in toad skin.These findings may in turn have implications for further drug development from traditional Chinese medicine sources.
文摘VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore, anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.
基金Sponsored by the National Natural Science Foundation and Civil Aviation Administration of China (Grant No. 61101122)the Fundamental Research Funds for the Central Universities (Grant No. HIT. NSRIF. 2010090)+1 种基金the China Postdoctoral Science Foundation (Grant No. 20100471079)the Heilongjiang Province Postdoctoral Science Foundation (Grant No. LBH-z10127)
文摘Currently,the WLAN indoor positioning system attracts a lot of interests,not only because of the cheap implementation but also because of the high positioning accuracy comparing with other indoor positioning systems.The WLAN indoor positioning system contains two phases,which are offline phase and online phase.In the online phase,the WLAN equipment user(UE) has to access to the WLAN for the latest radio map and positioning software.Due to during the network allocation vector(NAV) duration,the WLAN channel is only reserved for one WLAN UE,others UEs' carrier accessing will be blocked.In addition,the blocked UE will make a retrial accessing,which will definitely introduce more traffic blocking to the WLAN.So In this paper,based on the analysis of the WLAN indoor positioning system architecture,a proper queuing model by using of the Extended Erlang B formula is proposed,which takes the retrial calling percentage into consideration in the proposed model.The simulation results show that the proposed method is more accurate and performs well to predict the blocking probability.
基金Supported by Grants from the Ministry of Health,Labour and Welfare(21-1)the Japan Society for the Promotion of Science (22590356 and 22790378)+3 种基金the Hamamatsu Foundation for Science and Technology Promotion,the Ministry of Education, Culture,Sports,Science and Technology(221S0001)the Takeda Science Foundationthe Aichi Cancer Research Foundationthe Smoking Research Foundation
文摘AIM:To investigate the suppressive activity of MUTYH variant proteins against mutations caused by oxidative lesion,8-hydroxyguanine(8OHG),in human cells.METHODS:p.R154H,p.M255V,p.L360P,and p.P377L MUTYH variants,which were previously found in patients with colorectal polyposis and cancer,were selected for use in this study.Human H1299 cancer cell lines inducibly expressing wild-type(WT) MUTYH(type 2) or one of the 4 above-mentioned MUTYH variants were established using the piggyBac transposon vector system,enabling the genomic integration of the transposon sequence for MUTYH expression.MUTYH expression was examined after cumate induction using Western blotting analysis and immunofluorescence analysis.The intracellular localization of MUTYH variants tagged with FLAG was also immunofluorescently examined.Next,the mutation frequency in the supF of the shuttle plasmid pMY189 containing a single 8OHG residue at position 159 of the supF was compared between empty vector cells and cells expressing WT MUTYH or one of the 4 MUTYH variants using a supF forward mutation assay.RESULTS:The successful establishment of human cell lines inducibly expressing WT MUTYH or one of the 4 MUTYH variants was concluded based on the detection of MUTYH expression in these cell lines after treatment with cumate.All of the MUTYH variants and WT MUTYH were localized in the nucleus,and nuclear localization was also observed for FLAG-tagged MUTYH.The mutation frequency of supF was 2.2 × 10-2 in the 8OHG-containing pMY189 plasmid and 2.5 × 10-4 in WT pMY189 in empty vector cells,which was an 86-fold increase with the introduction of 8OHG.The mutation frequency(4.7 × 10-3) of supF in the 8OHG-containing pMY189 plasmid in cells overexpressing WT MUTYH was significantly lower than in the empty vector cells(P < 0.01).However,the mutation frequencies of the supF in the 8OHG-containing pMY189 plasmid in cells overexpressing the p.R154H,p.M255V,p.L360P,or p.P377L MUTYH variant were 1.84 × 10-2,1.55 × 10-2,1.91 × 10-2,and 1.96 × 10-2,respectively,meaning that no significant difference was observed in the mutation frequency between the empty vector cells and cells overexpressing MUTYH mutants.CONCLUSION:The suppressive activities of p.R154H,p.M255V,p.L360P,and p.P377L MUTYH variants against mutations caused by 8OHG are thought to be severely impaired in human cells.
文摘Stress analysis of cylindrical grid-stiffened composite shells was conducted under transverse loading,pure bending,torsion and axial compression under clamped-free boundary condition.Electrical strain gauges were employed to measure the strains in transverse loading case to validate the finite element analysis which was conducted using ANSYS software.Good agreement was obtained between the two methods.It was observed that stiffening the composite shell with helical ribs decreased the average equivalent Von Mises stress on the shell.The reduction of the stress seemed to be higher in the intersection of two ribs.It was also seen that the stress reduction ratio was higher when the structure was under bending compared to torsion and axial compression.The reduction ratio was approximately 75% in pure bending in the intersection point of the ribs,while it was approximately 25% in torsion.Therefore,it is concluded that the presence of the ribs is more effective under bending.Failure analysis was done using Tsai-Wu criterion.The ribs were observed to result in maximum and minimum increase in the failure load of the structure under transverse bending and torsional loading,respectively.
文摘The first decision we need to make in a structural load assessment is what approach should be applied, a linear approach or a non-linear one. The correct decision comes from understanding of the technology used in the linear and non-linear approaches and also comes from the understanding of the problem to he analyzed. From engineering practice, it has been found that many non-linear effects can be taken into account in a linear model with appropriate approach. A study of hydrodynamic structural load on a stinger of a pipe-laying vessel is presented in this paper. The results of a non-linear analysis are compared to those of linear models with different approaches, and how the nonlinear effect can be involved in a linear model is discussed. The recommendations on how to estimate the non-linear effects in a linear structural load model is discussed.
基金supported by the National Natural Science Foundation of China (20973079 & 21003107)State Basic Research Project of China(2009CB623507)Fundamental Research Funds for the Central Universities (2010QNA3035)
文摘Porous polymer supported palladium catalyst for cross coupling reactions with high activity has been successfully prepared by coordination of Pd 2+ species with Schiff bases functionalized porous polymer. The catalyst has been systemically investi-gated by a series of characterizations such as TEM, N 2 adsorption, NMR, IR, XPS, etc. TEM and N 2 isotherms show that the sample maintains the nanoporous structure after the modification and coordination. XPS results show that chemical state of palladium species in the catalyst is mainly +2. More importantly, the catalyst shows very high activities and excellent recycla-bility in a series of coupling reactions including Suzuki, Sonogashira, and Heck reactions. Hot filtration and poison of catalysts experiments have also been performed and the results indicate that soluble active species (mainly Pd(0) species) in-situ gener-ated from the catalyst under the reaction conditions are the active intermediates, which would redeposit to the supporter after the reactions.
