目的探讨胆盐载体MRP1、MRP2在妊娠期肝内胆汁淤积症(ICP)胎盘的表达,分析胎盘胆盐载体与ICP发病的关系。方法收集8例正常早孕妇女绒毛组织、7例正常中孕妇女胎盘组织、20例正常晚孕妇女(对照组)胎盘组织,以及20例ICP患者胎盘组织,检测...目的探讨胆盐载体MRP1、MRP2在妊娠期肝内胆汁淤积症(ICP)胎盘的表达,分析胎盘胆盐载体与ICP发病的关系。方法收集8例正常早孕妇女绒毛组织、7例正常中孕妇女胎盘组织、20例正常晚孕妇女(对照组)胎盘组织,以及20例ICP患者胎盘组织,检测收集组织中胆盐载体MRP1和MRP2 mRNA的表达。结果ICP和正常妊娠各期胎盘组织中均有MRP2 mRNA的表达,而MRP1 mRNA在大部分标本上有表达;ICP组MRP1 mRNA和MRP2 mRNA表达量与对照组相比(99.94±73.17 vs 99.20±68.65;95.78±56.50 vs 142.20±91.27)差异无统计学意义(P>0.05);ICP组未用地塞米松治疗胎盘MRP2 mRNA表达量低于对照组(91.82±48.08 vs 142.20±91.27,P<0.05)。结论在未用地塞米松治疗的ICP患者胎盘组织中,MRP2 mRNA的表达量降低,这可能是引起ICP患者胎盘胆汁酸转运障碍,从而引起胎儿体内胆汁淤积的机制之一。展开更多
Liposomes were prepared with natural soybean phospholipids by extrusion method after rotating-film evaporating technique. Transmission electron micrography was used to detect the appearances of the prepared liposomes,...Liposomes were prepared with natural soybean phospholipids by extrusion method after rotating-film evaporating technique. Transmission electron micrography was used to detect the appearances of the prepared liposomes, and the liposome diameter was also measured. The prepared liposomes were sphere in shape with the mean diameter of 217 nm and span of 0.838. The phospholipid bilayer structure, suitable for entrapping various effector molecules, could be seen clearly under transmission electron microscopy. The bile salts of sodium cholate and sodium deoxycholate were used as the surfactants to investigate their interaction with liposomes. The turbidities for the mixture of bile salts and liposomes were evaluated by the visible spectrometry method at the wavelength of 500 nm. And the diameter changes of liposomes were also tested to examine the effect of bile salts on liposomes. At the beginning, the diameters and turbidities of liposomes increased a little as the result of mixed micelles formation during the different stages for the structure changes of surfactant-liposomes micelles. The further added bile salts decreased the diameters and turbidities of liposomes. The liposome suspension underwent several rearrangements before small mixed micelles formed. And the diameter of liposomes changed regularly. The interaction of bile salts and liposomes is important for the further study of the behaviors of liposomes in vivo. The drug loaded and release properties of liposomes can also be well reflected by the interaction of liposomes and surfactants.展开更多
Novel hollow Fe3O4 nanoparticles for drug delivery were synthesized via a one-step template- free approach. These nanoparticles were obtained by modifing the Fe3O4 nanoparticles with 3-aminopropyltrimethoxy silane, an...Novel hollow Fe3O4 nanoparticles for drug delivery were synthesized via a one-step template- free approach. These nanoparticles were obtained by modifing the Fe3O4 nanoparticles with 3-aminopropyltrimethoxy silane, and then grafting alginate onto the surface of amine magnetic. The hollow structure of Fe3O4 spheres was characterized by TEM, XRD, and XPS. The M-H hysteresis loop indicated that the magnetic spheres exhibit snperparamagnetic characteristics at room temperature. Daunorubicin acting as a model drug was loaded into the carrier, and the maximum percent of envelop and load were 28.4% and 14.2% respectively. The drug controlled releasing behaviors of the carriers were compared in different pH media.展开更多
A porous Me2-CA-BTP/SiO2-P adsorbent was prepared to separate MA（III） from Ln（III） in high level liquid waste （HLLW）. The adsorption behavior of Me2-CA-BTP/SiO2-P toward 241Am（III） and Ln（III） in 0.01 M HNO3...A porous Me2-CA-BTP/SiO2-P adsorbent was prepared to separate MA（III） from Ln（III） in high level liquid waste （HLLW）. The adsorption behavior of Me2-CA-BTP/SiO2-P toward 241Am（III） and Ln（III） in 0.01 M HNO3-NaNO3 solution was studied. Me2-CA-BTP/SiO2-P showed high adsorption and selectivity toward 241Am（III） over Ln（III） fission products with the separation factor （SF） reaching to 557, 2355, 1952, 1082, 214, 105, 86, 14 for Y, La, Ce, Nd, Sm, Eu, Gd and Dy respectively in 0.01 M HNO3-0.99 M NaNO3 solution. The adsorption kinetics of both Dy（III） and Eu（III） on Me2-CA-BTP/SiO2-P was studied and followed pseudo-second-order rate equation indicating chemical sorption as the rate-limiting step of the adsorption, and the adsorption isotherm of Dy（III） and Eu（III） matched better with the Langmuir isotherm than the Freundlich isotherm with the adsorption amount around 0.22 and 0.20 mmol/g respectively. Thermodynamic study revealed that the adsorption of both Dy（III） and Eu（III） on Me2-CA-BTP/SiO2-P was spontaneous and endothermic processes with a positive entropy at 298, 308, 313 K.展开更多
文摘目的探讨胆盐载体MRP1、MRP2在妊娠期肝内胆汁淤积症(ICP)胎盘的表达,分析胎盘胆盐载体与ICP发病的关系。方法收集8例正常早孕妇女绒毛组织、7例正常中孕妇女胎盘组织、20例正常晚孕妇女(对照组)胎盘组织,以及20例ICP患者胎盘组织,检测收集组织中胆盐载体MRP1和MRP2 mRNA的表达。结果ICP和正常妊娠各期胎盘组织中均有MRP2 mRNA的表达,而MRP1 mRNA在大部分标本上有表达;ICP组MRP1 mRNA和MRP2 mRNA表达量与对照组相比(99.94±73.17 vs 99.20±68.65;95.78±56.50 vs 142.20±91.27)差异无统计学意义(P>0.05);ICP组未用地塞米松治疗胎盘MRP2 mRNA表达量低于对照组(91.82±48.08 vs 142.20±91.27,P<0.05)。结论在未用地塞米松治疗的ICP患者胎盘组织中,MRP2 mRNA的表达量降低,这可能是引起ICP患者胎盘胆汁酸转运障碍,从而引起胎儿体内胆汁淤积的机制之一。
文摘Liposomes were prepared with natural soybean phospholipids by extrusion method after rotating-film evaporating technique. Transmission electron micrography was used to detect the appearances of the prepared liposomes, and the liposome diameter was also measured. The prepared liposomes were sphere in shape with the mean diameter of 217 nm and span of 0.838. The phospholipid bilayer structure, suitable for entrapping various effector molecules, could be seen clearly under transmission electron microscopy. The bile salts of sodium cholate and sodium deoxycholate were used as the surfactants to investigate their interaction with liposomes. The turbidities for the mixture of bile salts and liposomes were evaluated by the visible spectrometry method at the wavelength of 500 nm. And the diameter changes of liposomes were also tested to examine the effect of bile salts on liposomes. At the beginning, the diameters and turbidities of liposomes increased a little as the result of mixed micelles formation during the different stages for the structure changes of surfactant-liposomes micelles. The further added bile salts decreased the diameters and turbidities of liposomes. The liposome suspension underwent several rearrangements before small mixed micelles formed. And the diameter of liposomes changed regularly. The interaction of bile salts and liposomes is important for the further study of the behaviors of liposomes in vivo. The drug loaded and release properties of liposomes can also be well reflected by the interaction of liposomes and surfactants.
文摘Novel hollow Fe3O4 nanoparticles for drug delivery were synthesized via a one-step template- free approach. These nanoparticles were obtained by modifing the Fe3O4 nanoparticles with 3-aminopropyltrimethoxy silane, and then grafting alginate onto the surface of amine magnetic. The hollow structure of Fe3O4 spheres was characterized by TEM, XRD, and XPS. The M-H hysteresis loop indicated that the magnetic spheres exhibit snperparamagnetic characteristics at room temperature. Daunorubicin acting as a model drug was loaded into the carrier, and the maximum percent of envelop and load were 28.4% and 14.2% respectively. The drug controlled releasing behaviors of the carriers were compared in different pH media.
基金supported by the National Natural Science of China(91126006,11305102)the Ministry of Education of Specialized Research Fund for the Doctoral Program of Higher Education (20130073110046)
文摘A porous Me2-CA-BTP/SiO2-P adsorbent was prepared to separate MA（III） from Ln（III） in high level liquid waste （HLLW）. The adsorption behavior of Me2-CA-BTP/SiO2-P toward 241Am（III） and Ln（III） in 0.01 M HNO3-NaNO3 solution was studied. Me2-CA-BTP/SiO2-P showed high adsorption and selectivity toward 241Am（III） over Ln（III） fission products with the separation factor （SF） reaching to 557, 2355, 1952, 1082, 214, 105, 86, 14 for Y, La, Ce, Nd, Sm, Eu, Gd and Dy respectively in 0.01 M HNO3-0.99 M NaNO3 solution. The adsorption kinetics of both Dy（III） and Eu（III） on Me2-CA-BTP/SiO2-P was studied and followed pseudo-second-order rate equation indicating chemical sorption as the rate-limiting step of the adsorption, and the adsorption isotherm of Dy（III） and Eu（III） matched better with the Langmuir isotherm than the Freundlich isotherm with the adsorption amount around 0.22 and 0.20 mmol/g respectively. Thermodynamic study revealed that the adsorption of both Dy（III） and Eu（III） on Me2-CA-BTP/SiO2-P was spontaneous and endothermic processes with a positive entropy at 298, 308, 313 K.
