For rigid-flexible coupling multi-body with variable topology,such as the system of internally carried air-launched or heavy cargo airdrop,in order to construct a dynamic model with unified form,avoid redundancy in th...For rigid-flexible coupling multi-body with variable topology,such as the system of internally carried air-launched or heavy cargo airdrop,in order to construct a dynamic model with unified form,avoid redundancy in the modeling process and make the solution independent,a method based on the equivalent rigidization model was proposed.It divides a system into independent subsystems by cutting off the joints,of which types are changed with the operation process of the system.And models of different subsystems can be constructed via selecting suitable modeling methods.Subsystem models with flexible bodies are on the basis of the equivalent rigidization model which replaces the flexible bodies with the virtual rigid bodies.And the solution for sanction,which is based on the constraints force algorithm(CFA)and vector mechanics,can be independent on the state equations.The internally carried air-launched system was taken as an example for verifying validity and feasibility of the method and theory.The dynamic model of aircraft-rocket-parachute system in the entire phase was constructed.Comparing the modeling method with the others,the modeling process was programmed;and form of the model is unified and simple.The model,method and theory can be used to analyze other similar systems such as heavy cargo airdrop system and capsule parachute recovery system.展开更多
In cattle, dietary protein is gradually degraded into peptide-bound amino acids（PBAAs）, free amino acids（FAAs）, and ultimately into ammonia by the rumen microbes. Both PBAA and FAA are milk protein precursors, and...In cattle, dietary protein is gradually degraded into peptide-bound amino acids（PBAAs）, free amino acids（FAAs）, and ultimately into ammonia by the rumen microbes. Both PBAA and FAA are milk protein precursors, and the rumen and small intestines are the main sites where such precursors are produced and absorbed. This work was designed to investigate the expression of the peptide transporter Pep T1 and the AA transporters ASCT2, y＋LAT1, and ATB0,＋, and the concentrations of PBAA, FAA, and soluble protein in the rumen, omasum, and duodenum of dairy cows. Tissues and digesta were collected from six healthy Chinese Holstein dairy cows immediately after the animals were slaughtered. The expression of transporters was analyzed by real-time quantitative polymerase chain reaction（PCR）. The FAA concentration was assessed using an amino acid（AA） analyzer, PBAA concentration by quantification of AA before and after acid-hydrolysis by 6 mol/L HCl, and soluble protein concentration by quantification of the bicinchoninic acid content. The results showed that the relative abundance of m RNA of the transporters and the soluble non-ammonia nitrogen（SNAN） concentration of each fraction were greater in the duodenum than in the rumen or omasum. These results indicate that the duodenum is the predominant location within the nonmesenteric digestive tract for producing milk protein precursors. In addition, PBAA was the largest component of SNAN in the digesta from the rumen, omasum, and duodenum. In conclusion, the duodenum has the greatest concentrations of SNAN and PBAA, and the greatest potential for absorption of SNAN in the form of PBAA in the nonmesenteric gastrointestinal tissues of dairy cows.展开更多
Normally, cellular responses to modified siRNAs or new siRNA delivery systems have been studied in group cell behavior by PCR, western blotting and fluorescence microscopy. In this study, we present a novel high-conte...Normally, cellular responses to modified siRNAs or new siRNA delivery systems have been studied in group cell behavior by PCR, western blotting and fluorescence microscopy. In this study, we present a novel high-content screening （HCS） strategy to evaluate a novel delivery system （named CLD） of siRNA therapeutics, with which both the content of intracellular siRNAs and changes in protein expressing levels have been quantified in group cells and cellular population. We also observed that with the better cell uptake, CLD provided siRNA therapeutics （siBraf） better antitumor capability. This novel strategy was proved to be with efficiency, accuracy and high competency to adherent cell lines, thus making siRNA research more simplified.展开更多
文摘For rigid-flexible coupling multi-body with variable topology,such as the system of internally carried air-launched or heavy cargo airdrop,in order to construct a dynamic model with unified form,avoid redundancy in the modeling process and make the solution independent,a method based on the equivalent rigidization model was proposed.It divides a system into independent subsystems by cutting off the joints,of which types are changed with the operation process of the system.And models of different subsystems can be constructed via selecting suitable modeling methods.Subsystem models with flexible bodies are on the basis of the equivalent rigidization model which replaces the flexible bodies with the virtual rigid bodies.And the solution for sanction,which is based on the constraints force algorithm(CFA)and vector mechanics,can be independent on the state equations.The internally carried air-launched system was taken as an example for verifying validity and feasibility of the method and theory.The dynamic model of aircraft-rocket-parachute system in the entire phase was constructed.Comparing the modeling method with the others,the modeling process was programmed;and form of the model is unified and simple.The model,method and theory can be used to analyze other similar systems such as heavy cargo airdrop system and capsule parachute recovery system.
基金Project supported by the National Basic Research Program(973)of China(No.2011CB100801)
文摘In cattle, dietary protein is gradually degraded into peptide-bound amino acids（PBAAs）, free amino acids（FAAs）, and ultimately into ammonia by the rumen microbes. Both PBAA and FAA are milk protein precursors, and the rumen and small intestines are the main sites where such precursors are produced and absorbed. This work was designed to investigate the expression of the peptide transporter Pep T1 and the AA transporters ASCT2, y＋LAT1, and ATB0,＋, and the concentrations of PBAA, FAA, and soluble protein in the rumen, omasum, and duodenum of dairy cows. Tissues and digesta were collected from six healthy Chinese Holstein dairy cows immediately after the animals were slaughtered. The expression of transporters was analyzed by real-time quantitative polymerase chain reaction（PCR）. The FAA concentration was assessed using an amino acid（AA） analyzer, PBAA concentration by quantification of AA before and after acid-hydrolysis by 6 mol/L HCl, and soluble protein concentration by quantification of the bicinchoninic acid content. The results showed that the relative abundance of m RNA of the transporters and the soluble non-ammonia nitrogen（SNAN） concentration of each fraction were greater in the duodenum than in the rumen or omasum. These results indicate that the duodenum is the predominant location within the nonmesenteric digestive tract for producing milk protein precursors. In addition, PBAA was the largest component of SNAN in the digesta from the rumen, omasum, and duodenum. In conclusion, the duodenum has the greatest concentrations of SNAN and PBAA, and the greatest potential for absorption of SNAN in the form of PBAA in the nonmesenteric gastrointestinal tissues of dairy cows.
基金Ministry of Science and Technology of China(Grant No.2012CB720604)NSFC(Grant No.20932001)
文摘Normally, cellular responses to modified siRNAs or new siRNA delivery systems have been studied in group cell behavior by PCR, western blotting and fluorescence microscopy. In this study, we present a novel high-content screening （HCS） strategy to evaluate a novel delivery system （named CLD） of siRNA therapeutics, with which both the content of intracellular siRNAs and changes in protein expressing levels have been quantified in group cells and cellular population. We also observed that with the better cell uptake, CLD provided siRNA therapeutics （siBraf） better antitumor capability. This novel strategy was proved to be with efficiency, accuracy and high competency to adherent cell lines, thus making siRNA research more simplified.
