AIM:To identify the prevalence of hepatitis B e antigen (HBeAg) and to assess the association of hepatitis B virus (HBV) core promoter mutations and viral load in Indonesian patients.METHODS:Sixty-four patients with c...AIM:To identify the prevalence of hepatitis B e antigen (HBeAg) and to assess the association of hepatitis B virus (HBV) core promoter mutations and viral load in Indonesian patients.METHODS:Sixty-four patients with chronic hepatitis,65 with liver cirrhosis and 50 with hepatocellular carcinoma were included in this study.HBeAg and hepatitis B e antibody (HBeAb) tests were performed using enzyme-linked immunosorbent assay and the mutations were analyzed by sequencing.Viral load was measured by real-time polymerase chain reaction.RESULTS:Of 179 patients,108 (60.3%) were HBeAg(-) and 86 (79.6%) of these HBeAg(-) patients had been seroconverted.The A1896 mutation was not found in HBeAg(+) patients,however,this mutation was detected in 70.7% of HBeAg(-) patients.This mutation was frequently found when HBeAg was not expressed (87.7%),compared to that found in HBeAg seroconverted patients (65.1%).The A1899 mutation was also more prevalent in HBeAg(-) than in HBeAg(+) patients (P=0.004).The T1762/A1764 mutation was frequently found in both HBeAg(+) and HBeAg(-) patients,however,the prevalence of this mutation did not significantly differ among the two groups (P=0.054).In HBeAg(+) patients,the T1762/A1764 mutation was correlated with lower HBV DNA (P < 0.001).The A1899 mutation did not correlate with HBV DNA (P=0.609).In HBeAg(-) patients,the T1762/A1764 mutation alone was not correlated with HBV DNA (P=0.095),however,the presence of either the T1762/A1764 or A1896 mutations was associated with increased HBV DNA (P < 0.001).CONCLUSION:The percentage of HBeAg(-) patients is high in Indonesia,and most of the HBeAg(-) patients had been seroconverted.The A1896 mutation was most likely the major cause of HBeAg loss.The T1762/A1764 mutation alone was associated with lower viral loads in HBeAg(+) patients,but not in HBeAg(-) patients.展开更多
The current study aims to evaluate the dynamic response of stabilized cohesive soil using an enzymatic preparation in terms of resilient modulus.We ran a series of resilient modulus testing according to AASHTO T307 on...The current study aims to evaluate the dynamic response of stabilized cohesive soil using an enzymatic preparation in terms of resilient modulus.We ran a series of resilient modulus testing according to AASHTO T307 on three types of cohesive soil treated with an enzymatic preparation to investigate its potential on roads construction.The results show significant improvement in the resilient modulus values,estimated at 1.4 to 4.4 times that observed for the untreated soil.Because of the complexity in conducting the resilient modulus measurement,we did a regression analysis to produce reliable correlation formula to predict the resilient modulus for untreated and stabilised soil samples involving stress state.The resilient modulus values for the subgrade materials at the anticipated field stresses were determined using a universal model.The enzymatic preparation was applied in pavement of a sample road and evaluated using the plate load test.SEM analysis for soil samples shows improvement in the soil compaction via reduction of voids between soil particles.XRD analysis shows no major structural changes in the treated soils.The enzymatic preparation contains 43 mg/mL of proteins.We used the SDS-PAGE(sodium dodecyl sulphate polyacrylamide gel electrophoresis)technique to identify the main protein components;however,the presence of interfering materials(surfactants)hinders the separation.展开更多
基金Supported by MRIN Funding (Budget No.cc041/2009)
文摘AIM:To identify the prevalence of hepatitis B e antigen (HBeAg) and to assess the association of hepatitis B virus (HBV) core promoter mutations and viral load in Indonesian patients.METHODS:Sixty-four patients with chronic hepatitis,65 with liver cirrhosis and 50 with hepatocellular carcinoma were included in this study.HBeAg and hepatitis B e antibody (HBeAb) tests were performed using enzyme-linked immunosorbent assay and the mutations were analyzed by sequencing.Viral load was measured by real-time polymerase chain reaction.RESULTS:Of 179 patients,108 (60.3%) were HBeAg(-) and 86 (79.6%) of these HBeAg(-) patients had been seroconverted.The A1896 mutation was not found in HBeAg(+) patients,however,this mutation was detected in 70.7% of HBeAg(-) patients.This mutation was frequently found when HBeAg was not expressed (87.7%),compared to that found in HBeAg seroconverted patients (65.1%).The A1899 mutation was also more prevalent in HBeAg(-) than in HBeAg(+) patients (P=0.004).The T1762/A1764 mutation was frequently found in both HBeAg(+) and HBeAg(-) patients,however,the prevalence of this mutation did not significantly differ among the two groups (P=0.054).In HBeAg(+) patients,the T1762/A1764 mutation was correlated with lower HBV DNA (P < 0.001).The A1899 mutation did not correlate with HBV DNA (P=0.609).In HBeAg(-) patients,the T1762/A1764 mutation alone was not correlated with HBV DNA (P=0.095),however,the presence of either the T1762/A1764 or A1896 mutations was associated with increased HBV DNA (P < 0.001).CONCLUSION:The percentage of HBeAg(-) patients is high in Indonesia,and most of the HBeAg(-) patients had been seroconverted.The A1896 mutation was most likely the major cause of HBeAg loss.The T1762/A1764 mutation alone was associated with lower viral loads in HBeAg(+) patients,but not in HBeAg(-) patients.
基金Project supported by the Academy of Scientific Research and Technology,ASRT,Cairo,Egypt
文摘The current study aims to evaluate the dynamic response of stabilized cohesive soil using an enzymatic preparation in terms of resilient modulus.We ran a series of resilient modulus testing according to AASHTO T307 on three types of cohesive soil treated with an enzymatic preparation to investigate its potential on roads construction.The results show significant improvement in the resilient modulus values,estimated at 1.4 to 4.4 times that observed for the untreated soil.Because of the complexity in conducting the resilient modulus measurement,we did a regression analysis to produce reliable correlation formula to predict the resilient modulus for untreated and stabilised soil samples involving stress state.The resilient modulus values for the subgrade materials at the anticipated field stresses were determined using a universal model.The enzymatic preparation was applied in pavement of a sample road and evaluated using the plate load test.SEM analysis for soil samples shows improvement in the soil compaction via reduction of voids between soil particles.XRD analysis shows no major structural changes in the treated soils.The enzymatic preparation contains 43 mg/mL of proteins.We used the SDS-PAGE(sodium dodecyl sulphate polyacrylamide gel electrophoresis)technique to identify the main protein components;however,the presence of interfering materials(surfactants)hinders the separation.
