Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To deter...Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25℃.展开更多
With the high availability of agricultural wastes in Malaysia, green energy is practical to be conducted. Thus, a research on the modification of the physical conditions of the solid state culture system, specifically...With the high availability of agricultural wastes in Malaysia, green energy is practical to be conducted. Thus, a research on the modification of the physical conditions of the solid state culture system, specifically different initial moisture content, fermentation temperature, inoculums size and different percentage of carbon and nitrogen sources were conducted to compare the usage of two different lignocellulosic materials which were oil palm frond and banana petiole respectively. After using a conventional method （one factor at a time）, the optimum fermentation conditions would be a combination of 70% initial moisture content （v/w）, at 28 ~C fermentation temperature with supplementation of 1% sucrose （w/w） and 1% peptone using 1 mL of inoculum in order to achieve highest xylanase production which was 1,711.87 U/mL and 1,579.60 U/mL for oil palm frond and banana petiole respectively. It was suggested that oil palm frond showed higher xylanase production compared to banana petiole.展开更多
基金Supported by Doctoral Initial Fund of Ludong University (No.43304)
文摘Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25℃.
文摘With the high availability of agricultural wastes in Malaysia, green energy is practical to be conducted. Thus, a research on the modification of the physical conditions of the solid state culture system, specifically different initial moisture content, fermentation temperature, inoculums size and different percentage of carbon and nitrogen sources were conducted to compare the usage of two different lignocellulosic materials which were oil palm frond and banana petiole respectively. After using a conventional method （one factor at a time）, the optimum fermentation conditions would be a combination of 70% initial moisture content （v/w）, at 28 ~C fermentation temperature with supplementation of 1% sucrose （w/w） and 1% peptone using 1 mL of inoculum in order to achieve highest xylanase production which was 1,711.87 U/mL and 1,579.60 U/mL for oil palm frond and banana petiole respectively. It was suggested that oil palm frond showed higher xylanase production compared to banana petiole.
