This article describes experimental evaluations of the authors＇ previous studies on the knowledge-based system （KBS） aimed at capturing and managing CNC （Computer Numerical Control） operator knowledge. The propos...This article describes experimental evaluations of the authors＇ previous studies on the knowledge-based system （KBS） aimed at capturing and managing CNC （Computer Numerical Control） operator knowledge. The proposed KBS follow thinking steps of CNC operators when they assess machining parameters described in the CAM （Computer Aided Manufacturing） file before proceeding to CNC machining processes. Also, the decision support system equipped with expert system （ES） has been proposed to realize efficient knowledge capturing system and effective usability of captured knowledge, and to recommend actions and decisions. From the viewpoint of providing useful information, the KBS should be aware of context or constraints in which the user has to deal with. In this study, the usefulness of DSS （Decision Support System） and ES is experimentally evaluated using real cases. Comparing the results of the testing participants with and without the DSS and ES, the effectiveness and usefulness were demonstrated. In addition, it was shown that the proposed system is also useful to narrow the discrepancies between CAM and CNC operators, focusing on CNC milling operations.展开更多
To investigate the role of CD4 + helper T (Th) cells in the memory CTL-mediated anti-tumor immunity, the RAG-1 gene knock out mice were adoptively transferred with OT-1 cells to generate the memory CTL, the C57BL/6 mi...To investigate the role of CD4 + helper T (Th) cells in the memory CTL-mediated anti-tumor immunity, the RAG-1 gene knock out mice were adoptively transferred with OT-1 cells to generate the memory CTL, the C57BL/6 mice immunized with the epitope peptide of OVA specific Th cells and with different adjuvants were adoptively transferred with these memory-CTLs, and then the animals were challenged with tumor cells EG7. It was found that although the simple immunization of mice with the epitope peptide of the OVA specific Th cells could generate more effect CTL, but this effect was not so strong enough to resist completely the challenges with tumor cells. Nevertheless, the memory CTL-mediated anti-tumor immune effect required the helps of Th1 and Th2 cells. The cross-regulation between Th1 and Th2 cells seemed to be beneficial for the host to generate more effector CTL for mounting an efficient anti-tumor response. It concluded that the interaction between Th1 and Th2 cells might be more important than the single subset of Th cells in the memory CTL-mediated anti-tumor immune response. More attention should be paid in this regard for the future studies.展开更多
文摘This article describes experimental evaluations of the authors＇ previous studies on the knowledge-based system （KBS） aimed at capturing and managing CNC （Computer Numerical Control） operator knowledge. The proposed KBS follow thinking steps of CNC operators when they assess machining parameters described in the CAM （Computer Aided Manufacturing） file before proceeding to CNC machining processes. Also, the decision support system equipped with expert system （ES） has been proposed to realize efficient knowledge capturing system and effective usability of captured knowledge, and to recommend actions and decisions. From the viewpoint of providing useful information, the KBS should be aware of context or constraints in which the user has to deal with. In this study, the usefulness of DSS （Decision Support System） and ES is experimentally evaluated using real cases. Comparing the results of the testing participants with and without the DSS and ES, the effectiveness and usefulness were demonstrated. In addition, it was shown that the proposed system is also useful to narrow the discrepancies between CAM and CNC operators, focusing on CNC milling operations.
文摘To investigate the role of CD4 + helper T (Th) cells in the memory CTL-mediated anti-tumor immunity, the RAG-1 gene knock out mice were adoptively transferred with OT-1 cells to generate the memory CTL, the C57BL/6 mice immunized with the epitope peptide of OVA specific Th cells and with different adjuvants were adoptively transferred with these memory-CTLs, and then the animals were challenged with tumor cells EG7. It was found that although the simple immunization of mice with the epitope peptide of the OVA specific Th cells could generate more effect CTL, but this effect was not so strong enough to resist completely the challenges with tumor cells. Nevertheless, the memory CTL-mediated anti-tumor immune effect required the helps of Th1 and Th2 cells. The cross-regulation between Th1 and Th2 cells seemed to be beneficial for the host to generate more effector CTL for mounting an efficient anti-tumor response. It concluded that the interaction between Th1 and Th2 cells might be more important than the single subset of Th cells in the memory CTL-mediated anti-tumor immune response. More attention should be paid in this regard for the future studies.
