The focusing tomography is presented to reconstruct 3 dimensional irradiance distribution. A 3 dimensional luminous body could be considered as the combination of many 2 dimensional parallel luminous sections. Focu...The focusing tomography is presented to reconstruct 3 dimensional irradiance distribution. A 3 dimensional luminous body could be considered as the combination of many 2 dimensional parallel luminous sections. Focus on these discrete sections respectively by a single camera, a group of images would be captured to form governing equations of irradiance. After inversion procedure, the irradiance distribution of different sections could be decoded. In this experimentation two lightbulbs are used to simulate two luminous sections. Reasonable results demonstrate that this technique could be a useful method in irradiance reconstruction after further development.展开更多
Geranylgeranyl diphosphate synthase （GGPPS） plays a key role in the biosynthesis of antioxidative carotenoid from the extremely radioresistant bacterium Deinococcus radiodurans. In this work, the recombinantGGPPS ex...Geranylgeranyl diphosphate synthase （GGPPS） plays a key role in the biosynthesis of antioxidative carotenoid from the extremely radioresistant bacterium Deinococcus radiodurans. In this work, the recombinantGGPPS expressed in Escherichia coli by cloning and transforming the gene dr1395 of D. radiodurans was isolated rapidly by an immobilized metal affinity supermacroporous cryogel, i.e., Cu2＋-iminodiacetic acid （IDA）-cryogel. The properties of the Cu2＋-IDA-cryogel were characterized using capillary-based mathematical model and experi- mental measurements. The obtained protein samples were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The porosity of the present Cu2＋-IDA-cryogel is 90.4% and the water permeability is 5.04x10-12 m2. From the capillary-based model, this cryogel presents a slightly wide normal pore （capillary） size distribution with the mean diameter of 55.2 μm, the standard deviation of 28.0 μm and the half of skeleton wall thickness of 2.8 μm. The pore size distribute from about 10 to 141 μm and the effective tortuosity of these capillary pores increases from 2.60 to 9.05. The isolation of the GGPPS from cell homogenate can be achieved at the flow velocity of 3.40x 10-4μm. s-1 by the Cu2＋-IDA-cryogel bed. High-purity GGPPS （about 91.4%） is obtained according to the SDS-PAGE analysis of the elution samples, indicating that the present method is a promising, simple and ef- fective atmroach to isolate GGPPS from cell homoenate of engineering, strains.展开更多
An unknown state of a quantum system S is usually determined by repeatedly measuring a set of non-commuting observables. The state can also be obtained from the repeated measurements of a single separable observable w...An unknown state of a quantum system S is usually determined by repeatedly measuring a set of non-commuting observables. The state can also be obtained from the repeated measurements of a single separable observable when the system S interacts with an assistant system A in a known state. In this paper, we study the quantum state tomography of a three-level atom （the system S） interacting with two radiation fields as the assistant system A. We obtain the initial state of S by repeatedly measuring a separable observable O = Sz n1 n2, in which Sz is the atom operator, and hi and h2 are the photon number operators of the two radiation fields. We achieve the one-to-one mapping M between the initial density matrix of the system S and the measured results of the single separable observable. We also give a concrete numerical example.展开更多
文摘The focusing tomography is presented to reconstruct 3 dimensional irradiance distribution. A 3 dimensional luminous body could be considered as the combination of many 2 dimensional parallel luminous sections. Focus on these discrete sections respectively by a single camera, a group of images would be captured to form governing equations of irradiance. After inversion procedure, the irradiance distribution of different sections could be decoded. In this experimentation two lightbulbs are used to simulate two luminous sections. Reasonable results demonstrate that this technique could be a useful method in irradiance reconstruction after further development.
基金Supported by the National Natural Science Foundation of China （30830006, 20876145, 21036005）, the International Science ＆ Technology Cooperation Program from the Ministry of Science and Technology of China （1017）, the Special Fund for Agroscientific Research in the Public Interest （201103007）, the Fundamental Research Funds for the Central Universities and the Natural Science Foundation of Zhejiang Province （Y4080326, Y407366）.
文摘Geranylgeranyl diphosphate synthase （GGPPS） plays a key role in the biosynthesis of antioxidative carotenoid from the extremely radioresistant bacterium Deinococcus radiodurans. In this work, the recombinantGGPPS expressed in Escherichia coli by cloning and transforming the gene dr1395 of D. radiodurans was isolated rapidly by an immobilized metal affinity supermacroporous cryogel, i.e., Cu2＋-iminodiacetic acid （IDA）-cryogel. The properties of the Cu2＋-IDA-cryogel were characterized using capillary-based mathematical model and experi- mental measurements. The obtained protein samples were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. The porosity of the present Cu2＋-IDA-cryogel is 90.4% and the water permeability is 5.04x10-12 m2. From the capillary-based model, this cryogel presents a slightly wide normal pore （capillary） size distribution with the mean diameter of 55.2 μm, the standard deviation of 28.0 μm and the half of skeleton wall thickness of 2.8 μm. The pore size distribute from about 10 to 141 μm and the effective tortuosity of these capillary pores increases from 2.60 to 9.05. The isolation of the GGPPS from cell homogenate can be achieved at the flow velocity of 3.40x 10-4μm. s-1 by the Cu2＋-IDA-cryogel bed. High-purity GGPPS （about 91.4%） is obtained according to the SDS-PAGE analysis of the elution samples, indicating that the present method is a promising, simple and ef- fective atmroach to isolate GGPPS from cell homoenate of engineering, strains.
基金the National Natural Science Foundation of China (Grant Nos. 60978009 and 91121023)the National Basic Research Program of China (Grant No. 2011CBA00200)
文摘An unknown state of a quantum system S is usually determined by repeatedly measuring a set of non-commuting observables. The state can also be obtained from the repeated measurements of a single separable observable when the system S interacts with an assistant system A in a known state. In this paper, we study the quantum state tomography of a three-level atom （the system S） interacting with two radiation fields as the assistant system A. We obtain the initial state of S by repeatedly measuring a separable observable O = Sz n1 n2, in which Sz is the atom operator, and hi and h2 are the photon number operators of the two radiation fields. We achieve the one-to-one mapping M between the initial density matrix of the system S and the measured results of the single separable observable. We also give a concrete numerical example.
