目的研究不同剂量电离辐射作用后多柔比星(阿霉素)对大肠癌多药耐药细胞株HCT-8细胞毒活性的影响,进一步探讨逆转大肠癌多药耐药性的方法。方法体外培养大肠癌细胞株HCT-8,以400 ng/m l阿霉素作为HCT-8细胞耐药模型刺激浓度制备细胞耐...目的研究不同剂量电离辐射作用后多柔比星(阿霉素)对大肠癌多药耐药细胞株HCT-8细胞毒活性的影响,进一步探讨逆转大肠癌多药耐药性的方法。方法体外培养大肠癌细胞株HCT-8,以400 ng/m l阿霉素作为HCT-8细胞耐药模型刺激浓度制备细胞耐药模型。实验模型分为5组:假照射组;大剂量组(2 G y);0.05 G y+2G y组;0.1 G y+2 G y组;0.2 G y+2 G y组。采用M TT法测定给予阿霉素后大肠癌多药耐药细胞株HCT-8的存活率。结果与假照射组相比,2 G y大剂量照射组及0.2 G y+2 G y组照射后HCT-8细胞存活率明显降低(P<0.05),先给予低剂量照射(0.05 G y,0.1 G y)后,再给予大剂量照射,HCT-8细胞存活率降低更明显(P<0.01)。与单纯2 G y大剂量照射组比较,0.1 G y+2 G y组HCT-8细胞存活率明显降低(P<0.05)。结论先给予低剂量照射(0.1 G y)后,再给予大剂量照射,可提高大肠癌多药耐药细胞株HCT-8对阿霉素的敏感性。展开更多
The Dark Matter Particle Explorer(DAMPE) is being constructed as a scientific satellite to observe high energy cosmic rays in space.As a crucial detector of DAMPE,the BGO calorimeter consists of 1848 PMT dynode signal...The Dark Matter Particle Explorer(DAMPE) is being constructed as a scientific satellite to observe high energy cosmic rays in space.As a crucial detector of DAMPE,the BGO calorimeter consists of 1848 PMT dynode signals which bring difficulties in front-end electronics on the space-limited and power-limited satellite platform.To overcome the challenge,a low-noise,low-power and high-integration ASIC chip,named VA32HDR14.2,is taken into account.In order to evaluate the radiation tolerance of the chip in space radiation environment,both single event effect(SEE) and total ionizing dose(TID) tests were performed.The SEE test result shows that the effective linear energy transfer(LET) threshold of single event latch-up(SEL) of the chip is around 23.0 MeVcm^2/mg,which is relatively sensitive,thus protection methods must be taken in the electronics design.The TID test result shows that the TID performance of the chip is higher than 25 Krad(Si),which satisfies the design specification.展开更多
文摘目的研究不同剂量电离辐射作用后多柔比星(阿霉素)对大肠癌多药耐药细胞株HCT-8细胞毒活性的影响,进一步探讨逆转大肠癌多药耐药性的方法。方法体外培养大肠癌细胞株HCT-8,以400 ng/m l阿霉素作为HCT-8细胞耐药模型刺激浓度制备细胞耐药模型。实验模型分为5组:假照射组;大剂量组(2 G y);0.05 G y+2G y组;0.1 G y+2 G y组;0.2 G y+2 G y组。采用M TT法测定给予阿霉素后大肠癌多药耐药细胞株HCT-8的存活率。结果与假照射组相比,2 G y大剂量照射组及0.2 G y+2 G y组照射后HCT-8细胞存活率明显降低(P<0.05),先给予低剂量照射(0.05 G y,0.1 G y)后,再给予大剂量照射,HCT-8细胞存活率降低更明显(P<0.01)。与单纯2 G y大剂量照射组比较,0.1 G y+2 G y组HCT-8细胞存活率明显降低(P<0.05)。结论先给予低剂量照射(0.1 G y)后,再给予大剂量照射,可提高大肠癌多药耐药细胞株HCT-8对阿霉素的敏感性。
基金Supported by the Strategic Priority Research Program on Space Science,the Chinese Academy of Sciences(No.XDA04040202-4)
文摘The Dark Matter Particle Explorer(DAMPE) is being constructed as a scientific satellite to observe high energy cosmic rays in space.As a crucial detector of DAMPE,the BGO calorimeter consists of 1848 PMT dynode signals which bring difficulties in front-end electronics on the space-limited and power-limited satellite platform.To overcome the challenge,a low-noise,low-power and high-integration ASIC chip,named VA32HDR14.2,is taken into account.In order to evaluate the radiation tolerance of the chip in space radiation environment,both single event effect(SEE) and total ionizing dose(TID) tests were performed.The SEE test result shows that the effective linear energy transfer(LET) threshold of single event latch-up(SEL) of the chip is around 23.0 MeVcm^2/mg,which is relatively sensitive,thus protection methods must be taken in the electronics design.The TID test result shows that the TID performance of the chip is higher than 25 Krad(Si),which satisfies the design specification.
