The highly conserved domain （exon 5-8） of p53 gene in transformed rat tracheal epithelial （RTE） cells was analyzed by means of polymerase chain reaction and single strand con formation polymorphism （PCR-SSCP）. T...The highly conserved domain （exon 5-8） of p53 gene in transformed rat tracheal epithelial （RTE） cells was analyzed by means of polymerase chain reaction and single strand con formation polymorphism （PCR-SSCP）. The result showed that single strand of exon 8 gene had mobility shift in polyacrylamide nondenaturing gel. DNA sequencing proved the mutation was G→C transversion at condon 265.展开更多
文摘The highly conserved domain （exon 5-8） of p53 gene in transformed rat tracheal epithelial （RTE） cells was analyzed by means of polymerase chain reaction and single strand con formation polymorphism （PCR-SSCP）. The result showed that single strand of exon 8 gene had mobility shift in polyacrylamide nondenaturing gel. DNA sequencing proved the mutation was G→C transversion at condon 265.
