[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds w...[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.展开更多
Rice is the source of important food in the world. The demand of rice tends to increase every year, thus research to increase genetic variation of rice by gamma irradiation has been conducted. This research aimed to s...Rice is the source of important food in the world. The demand of rice tends to increase every year, thus research to increase genetic variation of rice by gamma irradiation has been conducted. This research aimed to study the influence of gamma irradiation doses on the vegetative growth, yield and quality. The randomized completely block design was used with two factors --gamma irradiation doses and varieties. Two rice varieties were Ciherang and Cempo Ireng, while doses of gamma irradiation consisted of six levels： 0, 100, 200, 300, 400 and 500 Gy. The results indicated that the treatment of 200 Gy gamma irradiation to Ciherang could improve the number of grains/panicle, protein content, degraded plant height and amylose contents. Gamma irradiation to Cempo Ireng at dose of 200 Gy could improve the number of grains/panicle, 1,000 grain weight, while it decreased days to 50% heading and plant harvest age.展开更多
基金Supported by National Natural Science Foundation of China(308200204)Natural Science Research Program in Education Department of Henan Province(2009B180019)~~
文摘[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.
文摘Rice is the source of important food in the world. The demand of rice tends to increase every year, thus research to increase genetic variation of rice by gamma irradiation has been conducted. This research aimed to study the influence of gamma irradiation doses on the vegetative growth, yield and quality. The randomized completely block design was used with two factors --gamma irradiation doses and varieties. Two rice varieties were Ciherang and Cempo Ireng, while doses of gamma irradiation consisted of six levels： 0, 100, 200, 300, 400 and 500 Gy. The results indicated that the treatment of 200 Gy gamma irradiation to Ciherang could improve the number of grains/panicle, protein content, degraded plant height and amylose contents. Gamma irradiation to Cempo Ireng at dose of 200 Gy could improve the number of grains/panicle, 1,000 grain weight, while it decreased days to 50% heading and plant harvest age.
