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水稻miRNA应答低能N^+束辐照的基因芯片分析
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作者 周晓君 刘旭昊 +1 位作者 郭向萌 押辉远 《安徽农业科学》 CAS 北大核心 2011年第5期2567-2569,共3页
[目的]探讨水稻应答N+束辐照机制中相关miRNA的表达差异。[方法]各选取3组N+束辐照处理水稻种子和未处理的水稻种子,提取其在30℃发芽96 h幼苗总RNA,采用Agilent单通道水稻基因表达谱芯片筛选2组水稻幼苗差异表达基因。芯片中含有miRNA... [目的]探讨水稻应答N+束辐照机制中相关miRNA的表达差异。[方法]各选取3组N+束辐照处理水稻种子和未处理的水稻种子,提取其在30℃发芽96 h幼苗总RNA,采用Agilent单通道水稻基因表达谱芯片筛选2组水稻幼苗差异表达基因。芯片中含有miRNA探针510个,分析其在样品间表达差异2倍左右作为域值范围。[结果]辐照组与对照组相比表达差异显著的miRNA分子有14个,且均为下调。[结论]水稻N+束辐照组和对照组中存在差异表达的miRNA分子,可能调控某些基因的表达来应答离子辐照,从而表现出与对照不一样的生理生化和表型差异。 展开更多
关键词 N+束 辐照水稻 MIRNA 差异表达 基因芯片
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辐照改良水稻淀粉特性的研究 被引量:17
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作者 段智英 吴殿星 +2 位作者 沈圣泉 韩娟英 夏英武 《核农学报》 CAS CSCD 北大核心 2003年第4期249-254,共6页
以相似AAC的不同类型水稻 (籼稻、粳稻和杂交稻 )和不同AAC早籼稻品种为材料 ,研究了辐照对淀粉粘滞性、理化品质和淀粉颗粒的影响。研究表明 ,最高粘度 (PKV)、热浆粘度 (HPV)、冷胶粘度 (CPV)、消减值 (SBV)和回复值 (CSV)等 5个RVA... 以相似AAC的不同类型水稻 (籼稻、粳稻和杂交稻 )和不同AAC早籼稻品种为材料 ,研究了辐照对淀粉粘滞性、理化品质和淀粉颗粒的影响。研究表明 ,最高粘度 (PKV)、热浆粘度 (HPV)、冷胶粘度 (CPV)、消减值 (SBV)和回复值 (CSV)等 5个RVA谱特征参数随剂量增加均显著下降。辐照明显降低了低AAC品种、糯稻和中等AAC品种的AAC ,但对高AAC品种的AAC影响不显著。辐照对碱消值 (ASV)无影响 ,但最高粘度时间 (PKT)随剂量增加而下降。胶稠度尤其是高AAC的胶稠度 (GC)随剂量增加明显提高。胚乳内淀粉颗粒经辐照处理也有所变形。结果表明 ,辐照可提高稻米食用和蒸煮品质。 展开更多
关键词 辐照改良水稻 淀粉特性 粘滞性 理化品质 淀粉颗粒
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Gene Chip Analysis for Rice MicroRNA Response to Low Energy N^+ Beam Radiation
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作者 周晓君 刘旭昊 +1 位作者 郭向萌 押辉远 《Agricultural Science & Technology》 CAS 2010年第9期47-49,共3页
[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N+ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds w... [Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N+ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences. 展开更多
关键词 Nitrogen ion beam Irradiated rice MiRNA Differential expression Gene chip
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Improvement of Rice (Oryza sativa L.) var. Ciherang and Cempo Ireng Productivity Using Gamma Irradiation
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作者 Fitri Masruroh Samanhudi +1 位作者 Sulanjari Ahmad Yunus 《Journal of Agricultural Science and Technology(B)》 2016年第5期289-294,共6页
Rice is the source of important food in the world. The demand of rice tends to increase every year, thus research to increase genetic variation of rice by gamma irradiation has been conducted. This research aimed to s... Rice is the source of important food in the world. The demand of rice tends to increase every year, thus research to increase genetic variation of rice by gamma irradiation has been conducted. This research aimed to study the influence of gamma irradiation doses on the vegetative growth, yield and quality. The randomized completely block design was used with two factors --gamma irradiation doses and varieties. Two rice varieties were Ciherang and Cempo Ireng, while doses of gamma irradiation consisted of six levels: 0, 100, 200, 300, 400 and 500 Gy. The results indicated that the treatment of 200 Gy gamma irradiation to Ciherang could improve the number of grains/panicle, protein content, degraded plant height and amylose contents. Gamma irradiation to Cempo Ireng at dose of 200 Gy could improve the number of grains/panicle, 1,000 grain weight, while it decreased days to 50% heading and plant harvest age. 展开更多
关键词 Gamma irradiation rice (Oryza sativa L.) PRODUCTIVITY
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