Cp G ODN可增强疫苗特异性免疫反应,但是它在动物体内易降解和吞噬率低的特性降低其作为免疫佐剂的效果。本研究尝试用葡聚糖-精胺(DSP)包裹Cp G ODN,保护其免受核酸酶降解,提高细胞吞噬率。所合成DSP包裹Cp G ODN形成DSP-Cp G ODN复合...Cp G ODN可增强疫苗特异性免疫反应,但是它在动物体内易降解和吞噬率低的特性降低其作为免疫佐剂的效果。本研究尝试用葡聚糖-精胺(DSP)包裹Cp G ODN,保护其免受核酸酶降解,提高细胞吞噬率。所合成DSP包裹Cp G ODN形成DSP-Cp G ODN复合物,体外刺激脾脏淋巴细胞,ELISA法测量细胞培养上清中白介素12(IL-12)含量来分析复合物是否可更有效地激活免疫细胞。结果显示所合成DSP可完全包裹Cp G ODN形成有效粒径为500 nm的颗粒并降低核酸酶对Cp G ODN降解速率。在最佳包裹条件下DSP-Cp G ODN复合物所刺激的脾脏淋巴细胞IL-12分泌水平相比单独Cp G ODN组有15.5倍增加(p<0.001)。本研究表明DSP可望作为Cp G ODN输送载体,增强佐剂效果。展开更多
通过体外细胞实验,研究透明质酸钠/植物鞘氨醇/三肽-1经皮共输送纳米载体对皮肤屏障的修复作用。采用细胞实验考察透明质酸钠/植物鞘氨醇/三肽-1纳米载体对角质形成细胞(Ha Ca T)的增殖能力,细胞迁移能力,细胞分泌丝聚蛋白、水通道蛋白...通过体外细胞实验,研究透明质酸钠/植物鞘氨醇/三肽-1经皮共输送纳米载体对皮肤屏障的修复作用。采用细胞实验考察透明质酸钠/植物鞘氨醇/三肽-1纳米载体对角质形成细胞(Ha Ca T)的增殖能力,细胞迁移能力,细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平的影响。结果表明,与游离活性物比较,透明质酸钠/植物鞘氨醇/三肽-1纳米载体能显著提高Ha Ca T细胞增殖能力(P <0.05),增加Ha Ca T细胞迁移能力(P <0.01),且能显著促进HaCaT细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平(P <0.05)。说明透明质酸钠/植物鞘氨醇/三肽-1纳米载体具有修复皮肤屏障的作用,在新型高性能皮肤屏障修复护肤品领域具有良好的应用前景。展开更多
Hyaluronan (HA), the consistent glycosaminoglycans in extracellular matrix, is a kind of biomaterials with wonderful biocompatibility. To develop drug release system (DDS) with HA as drug carrier is a new hotspot in t...Hyaluronan (HA), the consistent glycosaminoglycans in extracellular matrix, is a kind of biomaterials with wonderful biocompatibility. To develop drug release system (DDS) with HA as drug carrier is a new hotspot in the field of pharmaceutics. In this paper, we applied technique of ultrosound and reversed phase (Water/Oil) emulsification to develop dexamethasone (DEX)-HA-STMP cross-linking microspheres (DEX-HA MS) with STMP as cross-linker. DEX-HA MS has a wonderful shape and property of dispersion. There is a negative correlation between diameter of DEX-HA MS and the content of cross-linker, or the content of emulsifier, and a positive correlation between the diameter and CHA . When CHA≤1%,DEX/HA≤1/10 (g/g),there is a positive correlation between the factors mentioned below and drug loading (DL%)/loading efficiency (LE%),the content of STMP, the content of emulsifier,CHA and the content of DEX. DEX-HA-MS can realize function of slow release. In vitro drug release experiment shows that cumulative release (CR%) of DEX-HA MS fits in with pervasion-corrosion equation, and there is a negative correlation between the content of STMP, CHA and CR%, a positive correlation between emulsifier and CR%. When DEX/HA≤1/5 (g/g) there is a negative correlation between the content of DEX and CR%.展开更多
文摘Cp G ODN可增强疫苗特异性免疫反应,但是它在动物体内易降解和吞噬率低的特性降低其作为免疫佐剂的效果。本研究尝试用葡聚糖-精胺(DSP)包裹Cp G ODN,保护其免受核酸酶降解,提高细胞吞噬率。所合成DSP包裹Cp G ODN形成DSP-Cp G ODN复合物,体外刺激脾脏淋巴细胞,ELISA法测量细胞培养上清中白介素12(IL-12)含量来分析复合物是否可更有效地激活免疫细胞。结果显示所合成DSP可完全包裹Cp G ODN形成有效粒径为500 nm的颗粒并降低核酸酶对Cp G ODN降解速率。在最佳包裹条件下DSP-Cp G ODN复合物所刺激的脾脏淋巴细胞IL-12分泌水平相比单独Cp G ODN组有15.5倍增加(p<0.001)。本研究表明DSP可望作为Cp G ODN输送载体,增强佐剂效果。
文摘通过体外细胞实验,研究透明质酸钠/植物鞘氨醇/三肽-1经皮共输送纳米载体对皮肤屏障的修复作用。采用细胞实验考察透明质酸钠/植物鞘氨醇/三肽-1纳米载体对角质形成细胞(Ha Ca T)的增殖能力,细胞迁移能力,细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平的影响。结果表明,与游离活性物比较,透明质酸钠/植物鞘氨醇/三肽-1纳米载体能显著提高Ha Ca T细胞增殖能力(P <0.05),增加Ha Ca T细胞迁移能力(P <0.01),且能显著促进HaCaT细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平(P <0.05)。说明透明质酸钠/植物鞘氨醇/三肽-1纳米载体具有修复皮肤屏障的作用,在新型高性能皮肤屏障修复护肤品领域具有良好的应用前景。
文摘Hyaluronan (HA), the consistent glycosaminoglycans in extracellular matrix, is a kind of biomaterials with wonderful biocompatibility. To develop drug release system (DDS) with HA as drug carrier is a new hotspot in the field of pharmaceutics. In this paper, we applied technique of ultrosound and reversed phase (Water/Oil) emulsification to develop dexamethasone (DEX)-HA-STMP cross-linking microspheres (DEX-HA MS) with STMP as cross-linker. DEX-HA MS has a wonderful shape and property of dispersion. There is a negative correlation between diameter of DEX-HA MS and the content of cross-linker, or the content of emulsifier, and a positive correlation between the diameter and CHA . When CHA≤1%,DEX/HA≤1/10 (g/g),there is a positive correlation between the factors mentioned below and drug loading (DL%)/loading efficiency (LE%),the content of STMP, the content of emulsifier,CHA and the content of DEX. DEX-HA-MS can realize function of slow release. In vitro drug release experiment shows that cumulative release (CR%) of DEX-HA MS fits in with pervasion-corrosion equation, and there is a negative correlation between the content of STMP, CHA and CR%, a positive correlation between emulsifier and CR%. When DEX/HA≤1/5 (g/g) there is a negative correlation between the content of DEX and CR%.
