聚双(2-吡咯基乙氧基磷腈)的合成、氧化交联和导电性研究

A kind of reactive inorganic polymer-poly(dichloro)phosphazene was prepared by ring-opening polymerization at a high temperature. Then a new conductive polyphosphazene containing pyrrole groups was synthesized by nucleophilic reaction between the inorganic polymer and sodium salts of N-hydroxyethylpyrrole. The chemical structure and properties of the polymers were characterized by 1H, 31P NMR, FTIR, DSC, TGA and GPC. The experimental results indicate that the molecular weights of pyrrole-containing soluble polymer are M n=4.1×10 5, M w=9.2×10 5( M w/ M n=2.24) and the T g is 237 K. A high qulity film was obtained from the soluble polymer through spin-coating method. Using FeCl 3 as oxidiant, the polymer film underwent crosslinking reaction to form conjugated polypyrrole. After the oxidation reaction, the color of polymer film turned into light black from almost transparent and the conductivity was determined to be 1.7×10 -5 S/cm, it is 1×10 5 times greater than that of the original film. The crosslinked polymer shows a high thermal stability.

偶氮聚电解质的H-聚集和光响应行为

用偶氮聚电解质上的偶氮苯基团作为“探针” ,研究了侧链偶氮聚电解质聚 {丙烯酸 2 [4 (4′ 乙氧基苯基偶氮 )苯氧基 ]乙酯 co 丙烯酸 }(PEAPE)在水中的H 聚集及其对光响应性的影响 .研究发现 ,与在DMF溶液的紫外吸收光谱相比 ,偶氮聚电解质在水中的紫外吸收λmax 发生明显蓝移 ,表明在水溶液中偶氮生色团形成了H 聚集体 .溶解在DMF H2 O混合溶剂中的上述偶氮聚电解质也存在部分H 聚集 ,H 聚集程度与水和DMF的比例有关 .H 聚集体的形成使光异构化速率明显减慢 ,异构化效率显著降低 .同时 ,其光异构化动力学不符合一级指数衰减规律 ,说明该过程同时包含‘孤立的’偶氮生色团的光异构化反应和H

SPE-HPLC法测定马破伤风免疫球蛋白F(ab')_2中Triton X-100残留量

目的：建立固相萃取高效液相色谱（SPE-HPLC）法测定马破伤风免疫球蛋白F（ab’）2中灭活剂Triton X-100的残留量。方法：采用Oasis固相萃取小柱对样品进行预处理,以水和5%甲醇为淋洗剂,以甲醇为洗脱剂,收集甲醇洗脱物用于HPLC分析。采用ZORBAX Extend-C18色谱柱（4.6 mm×150 mm,5μm）,流动相为甲醇-水（80∶20）,流速1.0 mL·min-1,检测波长230 nm,柱温30℃,进样量10μL。结果：Triton X-100浓度在0.56～55.34μg·mL-1范围内,与峰面积线性关系良好（r=0.9998）;检测限（S/N=3）为2.03 ng,定量限（S/N=10）为4.06 ng;提取回收率为91.8%。结论：本方法操作简便、准确、重复性好,可用于Triton X-100的残留量测定。