[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cl...[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cluster analysis and sub- ordinate function was conducted through determining related resistance indexes of 67 hot pepper germplasms 50 days after inoculated with M. incognita. [Result] The effects of M. incognita on related resistance indexes were significantly different am- ong the hot pepper germplasms. Egg index and gall index had abundant genetic variation with variation coefficients of 143.16% and 118.95%, respectively. Based on the gall indexes, cluster analysis of hot pepper germplasms was performed. The 67 hot pepper germplasms were divided into 4 groups （resistant, moderately resistant, susceptible and high susceptible）. The resistance intensity of the hot pepper germplasms were ranked according to the sum of subordinate function values of various resistance indexes. The total function values of Rela 2 and L506M were the largest （2.00）, indicating that these two germplasms were immune to M. incognita. The total function values of L287-2, L522-1M, L504M, L515-2, 13SM100-1, L512M, L292-1, L319, L316, L317, 13SM87-1 and Rela 5 were larger than 1.95, indicating that these germplasms were highly resistant to M. incognita. [Conclusion] This study could provide certain resistant resources for resistance breeding of hot pepper to M. incognita.展开更多
Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-res...Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-resistant pepper. [ Method ] With 110 pigment pepper seeds as the materials, their germination vigor under optimum temperature and suboptimal temperature were determined by means of roll rapid germination, and seeds with different genetic types were evaluated from aspects of germination vigor and its interval division. [ Result ] 37 pepper seeds with stronger low temperature resistance were screened. [ Conclusion]This study provides an important basis for screening low temperature-resistant pepper germplasm resources.展开更多
In this paper, the newly recorded species Ceratothripoides claratris （Shumsher） is described and illustrated. This species is an important vector of the Capsicum Chlorosis Virus. Slide-mounted specimens are deposite...In this paper, the newly recorded species Ceratothripoides claratris （Shumsher） is described and illustrated. This species is an important vector of the Capsicum Chlorosis Virus. Slide-mounted specimens are deposited at Yunnan Agricultural University.展开更多
[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Re...[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.展开更多
[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seawe...[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seaweed bio-organic fertilizer were prepared for seed soaking and pot incubation of cucumber, tomato and chili, to ob- serve the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of vegetables. [Result] Compared with the control, germination rate of cucumber and tomato seeds applied with 600-fold liquid seaweed bio-organic fer- tilizer varied significantly; germination rate of chili seeds applied with 400-fold liquid seaweed bio-organic fertilizer varied significantly; germination energy and germination index of chili seeds applied with different dilution concentrations of liquid seaweed bio-organic fertilizer presented no significant differences. In addition, 200-fold and 400-fold liquid seaweed bio-organic fertilizer significantly improved the root length, plant height, plant fresh weight, plant dry weight, chlorophyll content and leaf area of cucumber, tomato and chili seedlings; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content and leaf area of cucumber seedlings varied significantly compared with the control, but no significant differences were observed in plant height, plant fresh weight and plant dry weight; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content, plant height, plant fresh weight and plant dry weight of chili and tomato varied sig- nificantly compared with the control, but no significant differences were observed in leaf area. [Conclusion] Soaking vegetable seeds with liquid seaweed bio-organic fer- tilizer can significantly improve seed generation rate and seedling growth.展开更多
[Objective] This study aimed to predict the heterosis of hot pepper using SSR genetice distance. [Method] Twenty-five hybridized combinations between 10pepper parents were obtained through NCⅡ incomplete diallel cros...[Objective] This study aimed to predict the heterosis of hot pepper using SSR genetice distance. [Method] Twenty-five hybridized combinations between 10pepper parents were obtained through NCⅡ incomplete diallel cross to analyze the relationship between SSR genetic distance and heterosis. [Result] The genetic distance of the parents varied in a range from 0.13 to 0.33, and the average genetic distance was 0.25, indicating that the genetic difference was not obvious among the parents, namely, they shared a close genetic relationship. In addition, the SSR genetic distance was closely related to yield per plant and fruits per plant, but showed no obvious correlation with other traits. [Conclusion] This study proved that in a certain range the combinations between the parents with a distant genetic distance possessed stronger heterosis and a larger possibility to produce a high yield and vice versa.展开更多
[Objective] The aim was to research effects of high temperature stress on pepper yield by cultivation of peppers in different genotypes and provide theoretical references for pepper breeding and high-yield cultivation...[Objective] The aim was to research effects of high temperature stress on pepper yield by cultivation of peppers in different genotypes and provide theoretical references for pepper breeding and high-yield cultivation. [Method] Four pepper va- rieties were studied with varied genotypes to explore effects of temperatures on pepper fruiting and yield in the whole growth stage. [Result] The optimal-temperatre term for pepper blooming and fruiting were shorter. For example, the periods from June 16 to July 15 and from August 16 to September 15 would be the best, with temperature ranging from 20.70 ℃ to 30.74 ℃. In the stage from July 16 to August 15, the temperature range of 24.22 ℃-32.17 ℃ would severely affect pepper growth and yield. Pepper No. 1 and pepper No. 1-1＇s yields were just 38.21% and 51.74% of the yields in the stage 1st and 52.01% and 62.35% in the stage 3rd and eady No. 1 and late No. 1 were 48.1% and 72.38%, respectively. Under high tem- perature stress in summer, pepper No. 1, pepper No. 1-1, and late No. 1 showed extremely significant differences with early No. 1 （P〈0.01）. The yield ratios of pep- per No. 1 and pepper No. 1-1 in stage 1st （May 25-July 15） and the later three stages were 42.34：57.66 and 39.50：60.50; the ratio was 47.99：52.21 of early No. 1; the ratio of late No. 1 was 20.25：79.75. [Conclusion] The cultivation approaches should vary upon pepper variety, necessitating the focus on pepper management, fertilization, and irrigation, and locating peak-blooming term in moderate-temperature stage to accelerate pepper growth.展开更多
基金Supported by National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032014019,1630032015003)Key Research&Development Project of Hainan Province(ZDYF2016225)Key Technology Research and Demonstration Project of Farmland Improvement of Hainan Province(HNGDpz2015)
文摘[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cluster analysis and sub- ordinate function was conducted through determining related resistance indexes of 67 hot pepper germplasms 50 days after inoculated with M. incognita. [Result] The effects of M. incognita on related resistance indexes were significantly different am- ong the hot pepper germplasms. Egg index and gall index had abundant genetic variation with variation coefficients of 143.16% and 118.95%, respectively. Based on the gall indexes, cluster analysis of hot pepper germplasms was performed. The 67 hot pepper germplasms were divided into 4 groups （resistant, moderately resistant, susceptible and high susceptible）. The resistance intensity of the hot pepper germplasms were ranked according to the sum of subordinate function values of various resistance indexes. The total function values of Rela 2 and L506M were the largest （2.00）, indicating that these two germplasms were immune to M. incognita. The total function values of L287-2, L522-1M, L504M, L515-2, 13SM100-1, L512M, L292-1, L319, L316, L317, 13SM87-1 and Rela 5 were larger than 1.95, indicating that these germplasms were highly resistant to M. incognita. [Conclusion] This study could provide certain resistant resources for resistance breeding of hot pepper to M. incognita.
基金Supported by National Science and Technology Support Program"Key Technology for male-sterile breeding of main vegetable cropsintegration and seed industrialization"(2008BADB1B04)SeedProject of Vegetable Germplasm and Breeding in Shandong Province"Start-up Funding of High-level Talents in Qingdao Agricultureuniversity"(630912)~~
文摘Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-resistant pepper. [ Method ] With 110 pigment pepper seeds as the materials, their germination vigor under optimum temperature and suboptimal temperature were determined by means of roll rapid germination, and seeds with different genetic types were evaluated from aspects of germination vigor and its interval division. [ Result ] 37 pepper seeds with stronger low temperature resistance were screened. [ Conclusion]This study provides an important basis for screening low temperature-resistant pepper germplasm resources.
基金supported by the Special Fund for Agro-Scientific Research in the Public Interest(Nos.200803025 and 201103026)Science and the Technique Program of Yunnan Province(No.2006SG23)the Natural Science Fund of Yunnan Province(No.2007C054M)
文摘In this paper, the newly recorded species Ceratothripoides claratris （Shumsher） is described and illustrated. This species is an important vector of the Capsicum Chlorosis Virus. Slide-mounted specimens are deposited at Yunnan Agricultural University.
基金Supported by Excellent Team Training Program of Yunnan Academy of Agriculture Sciences(YAAS2014YY002)~~
文摘[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.
文摘[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seaweed bio-organic fertilizer were prepared for seed soaking and pot incubation of cucumber, tomato and chili, to ob- serve the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of vegetables. [Result] Compared with the control, germination rate of cucumber and tomato seeds applied with 600-fold liquid seaweed bio-organic fer- tilizer varied significantly; germination rate of chili seeds applied with 400-fold liquid seaweed bio-organic fertilizer varied significantly; germination energy and germination index of chili seeds applied with different dilution concentrations of liquid seaweed bio-organic fertilizer presented no significant differences. In addition, 200-fold and 400-fold liquid seaweed bio-organic fertilizer significantly improved the root length, plant height, plant fresh weight, plant dry weight, chlorophyll content and leaf area of cucumber, tomato and chili seedlings; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content and leaf area of cucumber seedlings varied significantly compared with the control, but no significant differences were observed in plant height, plant fresh weight and plant dry weight; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content, plant height, plant fresh weight and plant dry weight of chili and tomato varied sig- nificantly compared with the control, but no significant differences were observed in leaf area. [Conclusion] Soaking vegetable seeds with liquid seaweed bio-organic fer- tilizer can significantly improve seed generation rate and seedling growth.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(200903025)Key Technologies Research and Development Program of Guizhou Province[(2011)3028+2 种基金(2011)3029]Special Fund of Guizhou Academy of Agricultural Sciences[(2009)018]Special Fund of Guizhou Province[(2012)2195]~~
文摘[Objective] This study aimed to predict the heterosis of hot pepper using SSR genetice distance. [Method] Twenty-five hybridized combinations between 10pepper parents were obtained through NCⅡ incomplete diallel cross to analyze the relationship between SSR genetic distance and heterosis. [Result] The genetic distance of the parents varied in a range from 0.13 to 0.33, and the average genetic distance was 0.25, indicating that the genetic difference was not obvious among the parents, namely, they shared a close genetic relationship. In addition, the SSR genetic distance was closely related to yield per plant and fruits per plant, but showed no obvious correlation with other traits. [Conclusion] This study proved that in a certain range the combinations between the parents with a distant genetic distance possessed stronger heterosis and a larger possibility to produce a high yield and vice versa.
基金Supported by Jiangsu Agricultural Science and Technology Self-raising Fund(00-05-10-30)~~
文摘[Objective] The aim was to research effects of high temperature stress on pepper yield by cultivation of peppers in different genotypes and provide theoretical references for pepper breeding and high-yield cultivation. [Method] Four pepper va- rieties were studied with varied genotypes to explore effects of temperatures on pepper fruiting and yield in the whole growth stage. [Result] The optimal-temperatre term for pepper blooming and fruiting were shorter. For example, the periods from June 16 to July 15 and from August 16 to September 15 would be the best, with temperature ranging from 20.70 ℃ to 30.74 ℃. In the stage from July 16 to August 15, the temperature range of 24.22 ℃-32.17 ℃ would severely affect pepper growth and yield. Pepper No. 1 and pepper No. 1-1＇s yields were just 38.21% and 51.74% of the yields in the stage 1st and 52.01% and 62.35% in the stage 3rd and eady No. 1 and late No. 1 were 48.1% and 72.38%, respectively. Under high tem- perature stress in summer, pepper No. 1, pepper No. 1-1, and late No. 1 showed extremely significant differences with early No. 1 （P〈0.01）. The yield ratios of pep- per No. 1 and pepper No. 1-1 in stage 1st （May 25-July 15） and the later three stages were 42.34：57.66 and 39.50：60.50; the ratio was 47.99：52.21 of early No. 1; the ratio of late No. 1 was 20.25：79.75. [Conclusion] The cultivation approaches should vary upon pepper variety, necessitating the focus on pepper management, fertilization, and irrigation, and locating peak-blooming term in moderate-temperature stage to accelerate pepper growth.
