[Objective] The aim was to research effects of high temperature stress on pepper yield by cultivation of peppers in different genotypes and provide theoretical references for pepper breeding and high-yield cultivation...[Objective] The aim was to research effects of high temperature stress on pepper yield by cultivation of peppers in different genotypes and provide theoretical references for pepper breeding and high-yield cultivation. [Method] Four pepper va- rieties were studied with varied genotypes to explore effects of temperatures on pepper fruiting and yield in the whole growth stage. [Result] The optimal-temperatre term for pepper blooming and fruiting were shorter. For example, the periods from June 16 to July 15 and from August 16 to September 15 would be the best, with temperature ranging from 20.70 ℃ to 30.74 ℃. In the stage from July 16 to August 15, the temperature range of 24.22 ℃-32.17 ℃ would severely affect pepper growth and yield. Pepper No. 1 and pepper No. 1-1＇s yields were just 38.21% and 51.74% of the yields in the stage 1st and 52.01% and 62.35% in the stage 3rd and eady No. 1 and late No. 1 were 48.1% and 72.38%, respectively. Under high tem- perature stress in summer, pepper No. 1, pepper No. 1-1, and late No. 1 showed extremely significant differences with early No. 1 （P〈0.01）. The yield ratios of pep- per No. 1 and pepper No. 1-1 in stage 1st （May 25-July 15） and the later three stages were 42.34：57.66 and 39.50：60.50; the ratio was 47.99：52.21 of early No. 1; the ratio of late No. 1 was 20.25：79.75. [Conclusion] The cultivation approaches should vary upon pepper variety, necessitating the focus on pepper management, fertilization, and irrigation, and locating peak-blooming term in moderate-temperature stage to accelerate pepper growth.展开更多
Objective To investigate the regulatory effects of nerve growth factor （NGF） on basal and capsaicin-induced release of neuropeptide substance P （SP） in primary cultured embryonic rat dorsal root ganglion （DRG） n...Objective To investigate the regulatory effects of nerve growth factor （NGF） on basal and capsaicin-induced release of neuropeptide substance P （SP） in primary cultured embryonic rat dorsal root ganglion （DRG） neurons. Methods DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF （10 ng/mL, 30 ng/mL, or 100 ng/mL） for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 （VR1） in the DRG neurons. The SP basal and capsaicin （100 nmol/L）-induced release in the culture were measured by radioimmunoassay （RIA）. Results SP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF. Conclusion NGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.展开更多
基金Supported by Jiangsu Agricultural Science and Technology Self-raising Fund(00-05-10-30)~~
文摘[Objective] The aim was to research effects of high temperature stress on pepper yield by cultivation of peppers in different genotypes and provide theoretical references for pepper breeding and high-yield cultivation. [Method] Four pepper va- rieties were studied with varied genotypes to explore effects of temperatures on pepper fruiting and yield in the whole growth stage. [Result] The optimal-temperatre term for pepper blooming and fruiting were shorter. For example, the periods from June 16 to July 15 and from August 16 to September 15 would be the best, with temperature ranging from 20.70 ℃ to 30.74 ℃. In the stage from July 16 to August 15, the temperature range of 24.22 ℃-32.17 ℃ would severely affect pepper growth and yield. Pepper No. 1 and pepper No. 1-1＇s yields were just 38.21% and 51.74% of the yields in the stage 1st and 52.01% and 62.35% in the stage 3rd and eady No. 1 and late No. 1 were 48.1% and 72.38%, respectively. Under high tem- perature stress in summer, pepper No. 1, pepper No. 1-1, and late No. 1 showed extremely significant differences with early No. 1 （P〈0.01）. The yield ratios of pep- per No. 1 and pepper No. 1-1 in stage 1st （May 25-July 15） and the later three stages were 42.34：57.66 and 39.50：60.50; the ratio was 47.99：52.21 of early No. 1; the ratio of late No. 1 was 20.25：79.75. [Conclusion] The cultivation approaches should vary upon pepper variety, necessitating the focus on pepper management, fertilization, and irrigation, and locating peak-blooming term in moderate-temperature stage to accelerate pepper growth.
文摘Objective To investigate the regulatory effects of nerve growth factor （NGF） on basal and capsaicin-induced release of neuropeptide substance P （SP） in primary cultured embryonic rat dorsal root ganglion （DRG） neurons. Methods DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF （10 ng/mL, 30 ng/mL, or 100 ng/mL） for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 （VR1） in the DRG neurons. The SP basal and capsaicin （100 nmol/L）-induced release in the culture were measured by radioimmunoassay （RIA）. Results SP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF. Conclusion NGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.
