Objective To investigate the regulatory effects of nerve growth factor （NGF） on basal and capsaicin-induced release of neuropeptide substance P （SP） in primary cultured embryonic rat dorsal root ganglion （DRG） n...Objective To investigate the regulatory effects of nerve growth factor （NGF） on basal and capsaicin-induced release of neuropeptide substance P （SP） in primary cultured embryonic rat dorsal root ganglion （DRG） neurons. Methods DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF （10 ng/mL, 30 ng/mL, or 100 ng/mL） for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 （VR1） in the DRG neurons. The SP basal and capsaicin （100 nmol/L）-induced release in the culture were measured by radioimmunoassay （RIA）. Results SP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF. Conclusion NGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.展开更多
Bolaxinhongxiu is a new cayenne pepper cultivar produced by crossing SJ008-5-2-1 (female parent) with 0622-1-3-2-1-1 (male parent). It can be used in its fresh form or as a processed product. Bolaxinhongxiu is a mid-m...Bolaxinhongxiu is a new cayenne pepper cultivar produced by crossing SJ008-5-2-1 (female parent) with 0622-1-3-2-1-1 (male parent). It can be used in its fresh form or as a processed product. Bolaxinhongxiu is a mid-maturing variety. Its first flower normally inserts in the 13th section;the plants are about 75 cm tall and 58 cm wide;the fruits are a group of tapering and mostly skinny peppers which colored jade green first and then turn tored-colored when ripe;they are about 28.5 cm long and 1.6 cm wide, often with bright but somewhat rippled skin;each pepper weighs around 28.7 g. Whether eaten raw or processed into chopped peppers or chili sauces, the fruits taste good with a strong pungent flavor. The plant has a vigorous root system and strong resistance to high temperature and drought;it is also highlyresistant to anthracnose, blight, and viral diseases. It is a cultivar with outstanding fruiting-rate and high yields, suitable for cultivation in mountains or the open fields along the Yangtze River Basin in spring and summer.展开更多
We prepared the Fe3O4/g‐C3N4nanoparticles(NPs)through a simple electrostatic self‐assembly method with a3:97weight ratio to investigate their Fenton,photo‐Fenton and oxidative functionalities besides photocatalytic...We prepared the Fe3O4/g‐C3N4nanoparticles(NPs)through a simple electrostatic self‐assembly method with a3:97weight ratio to investigate their Fenton,photo‐Fenton and oxidative functionalities besides photocatalytic functionality.We observed an improvement of the Fenton and photo‐Fenton activities of the Fe3O4/g‐C3N4nanocomposites.This improvement was attributed to efficient charge transfer between Fe3O4and g‐C3N4at the heterojunctions,inhibition of electron‐hole recombination,a high surface area,and stabilization of Fe3O4against leaching by the hydrophobic g‐C3N4.The obtained NPs showed a higher degradation potential for rhodamine B(RhB)dye than those of Fe3O4and g‐C3N4.As compared to photocatalysis,the efficiency of RhB degradation in the Fenton and photo‐Fenton reactions was increased by20%and90%,respectively.Additionally,the horseradish peroxidase(HRP)activity of the prepared nanomaterials was studied with3,3,5,5‐tetramethylbenzidinedihydrochloride(TMB)as a substrate.Dopamine oxidation was also examined.Results indicate that Fe3O4/g‐C3N4nanocomposites offers more efficient degradation of RhB dye in a photo‐Fenton system compared with regular photocatalytic degradation,which requires a long time.Our study also confirmed that Fe3O4/g‐C3N4nanocomposites can be used as a potential material for mimicking HRP owing to its high affinity for TMB.These findings suggest good potential for applications in biosensing and as a catalyst in oxidation reactions.展开更多
文摘Objective To investigate the regulatory effects of nerve growth factor （NGF） on basal and capsaicin-induced release of neuropeptide substance P （SP） in primary cultured embryonic rat dorsal root ganglion （DRG） neurons. Methods DRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF （10 ng/mL, 30 ng/mL, or 100 ng/mL） for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 （VR1） in the DRG neurons. The SP basal and capsaicin （100 nmol/L）-induced release in the culture were measured by radioimmunoassay （RIA）. Results SP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF. Conclusion NGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.
文摘Bolaxinhongxiu is a new cayenne pepper cultivar produced by crossing SJ008-5-2-1 (female parent) with 0622-1-3-2-1-1 (male parent). It can be used in its fresh form or as a processed product. Bolaxinhongxiu is a mid-maturing variety. Its first flower normally inserts in the 13th section;the plants are about 75 cm tall and 58 cm wide;the fruits are a group of tapering and mostly skinny peppers which colored jade green first and then turn tored-colored when ripe;they are about 28.5 cm long and 1.6 cm wide, often with bright but somewhat rippled skin;each pepper weighs around 28.7 g. Whether eaten raw or processed into chopped peppers or chili sauces, the fruits taste good with a strong pungent flavor. The plant has a vigorous root system and strong resistance to high temperature and drought;it is also highlyresistant to anthracnose, blight, and viral diseases. It is a cultivar with outstanding fruiting-rate and high yields, suitable for cultivation in mountains or the open fields along the Yangtze River Basin in spring and summer.
基金supported by the National Natural Science Foundation of China(51572253,21771171)Scientific Research Grant of Hefei Science Center of CAS(2015SRG-HSC048)+1 种基金cooperation between NSFC and Netherlands Organization for Scientific Research(51561135011)CAS-TWAS Scholarship Program~~
文摘We prepared the Fe3O4/g‐C3N4nanoparticles(NPs)through a simple electrostatic self‐assembly method with a3:97weight ratio to investigate their Fenton,photo‐Fenton and oxidative functionalities besides photocatalytic functionality.We observed an improvement of the Fenton and photo‐Fenton activities of the Fe3O4/g‐C3N4nanocomposites.This improvement was attributed to efficient charge transfer between Fe3O4and g‐C3N4at the heterojunctions,inhibition of electron‐hole recombination,a high surface area,and stabilization of Fe3O4against leaching by the hydrophobic g‐C3N4.The obtained NPs showed a higher degradation potential for rhodamine B(RhB)dye than those of Fe3O4and g‐C3N4.As compared to photocatalysis,the efficiency of RhB degradation in the Fenton and photo‐Fenton reactions was increased by20%and90%,respectively.Additionally,the horseradish peroxidase(HRP)activity of the prepared nanomaterials was studied with3,3,5,5‐tetramethylbenzidinedihydrochloride(TMB)as a substrate.Dopamine oxidation was also examined.Results indicate that Fe3O4/g‐C3N4nanocomposites offers more efficient degradation of RhB dye in a photo‐Fenton system compared with regular photocatalytic degradation,which requires a long time.Our study also confirmed that Fe3O4/g‐C3N4nanocomposites can be used as a potential material for mimicking HRP owing to its high affinity for TMB.These findings suggest good potential for applications in biosensing and as a catalyst in oxidation reactions.
