AIM: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. METHODS: A 19 200 gene/expressed sequence tag gen...AIM: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. METHODS: A 19 200 gene/expressed sequence tag gene chip was used to examine expression of genes after infection of Caco-2 cells with strains of normal flora E. coli,Lactobacillus plantarum,and a combination of the two. RESULTS: The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli . L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection,27 genes were up-regulated and 59 were down-regulated,with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. CONCLUSION: The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription,protein biosynthesis,metabolism,cell adhesion,ubiquitination,and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.展开更多
An ultra-wideband impulse radar was studied for the detection of buried life in coal mines. An improved Empirical Mode Decomposition (EMD) method based on a cross-correlation filter was proposed for reduction of multi...An ultra-wideband impulse radar was studied for the detection of buried life in coal mines. An improved Empirical Mode Decomposition (EMD) method based on a cross-correlation filter was proposed for reduction of multipath and noise interference. Multipath interference was first removed by cross-corre- lation filtering. Then the delays of each pulse in every echo were summed. An EMD algorithm was used for noise reduction for the total delay of each echo. The corresponding EMD results of every echo were then summed and averaged. Finally, evidence for the existence of buried life and their position were obtained from amplitude-frequency curves of the averaged EMD results. Detailed simulation experi- ments are presented to validate the effectiveness of this proposed method. The experimental results show that this method can efficiently eliminate multipath interference and reduce noise interference in echoes, which makes detection and location of buried life in coal mines more accurate.展开更多
Low-cost multistoried rental housing （rumah susun sederhana sewa, abbreviated to rusunawa） recently has become the main policy for the Indonesian government to provide adequate housing for low income people in the m...Low-cost multistoried rental housing （rumah susun sederhana sewa, abbreviated to rusunawa） recently has become the main policy for the Indonesian government to provide adequate housing for low income people in the metropolitan areas. In line with the policy, the government in the year 2005 has raised the technical life of building for low-cost multistoried rental housing from 30 years to 50 years. This study is conducted to assess variables that affect the technical life of architectural components, using Delphi study method involving stakeholders who have participated in the planning, design, construction, operation and maintenance of low-cost multistoried rental housing in Jakarta. Research findings contribute significantly to the assurance of technical life of architectural components in low-cost multistoried rental housing in Indonesia.展开更多
基金Supported by the Department of Pediatrics and GCRC (M01- RR-16500), University of Maryland Baltimore, with partial funding from NIH grants UO1 HD 40574 and RO1 HD 053719
文摘AIM: To investigate the change in eukaryotic gene expression profile in Caco-2 cells after infection with strains of Escherichia coli and commensal probiotic bacteria. METHODS: A 19 200 gene/expressed sequence tag gene chip was used to examine expression of genes after infection of Caco-2 cells with strains of normal flora E. coli,Lactobacillus plantarum,and a combination of the two. RESULTS: The cDNA microarray revealed up-regulation of 155 and down-regulation of 177 genes by E. coli . L. plantarum up-regulated 45 and down-regulated 36 genes. During mixed infection,27 genes were up-regulated and 59 were down-regulated,with nullification of stimulatory/inhibitory effects on most of the genes. Expression of several new genes was noted in this group. CONCLUSION: The commensal bacterial strains used in this study induced the expression of a large number of genes in colonocyte-like cultured cells and changed the expression of several genes involved in important cellular processes such as regulation of transcription,protein biosynthesis,metabolism,cell adhesion,ubiquitination,and apoptosis. Such changes induced by the presence of probiotic bacteria may shape the physiologic and pathologic responses they trigger in the host.
基金support forour work by the National Science and Technology Support Project of China (No. 2006BAK03B00)
文摘An ultra-wideband impulse radar was studied for the detection of buried life in coal mines. An improved Empirical Mode Decomposition (EMD) method based on a cross-correlation filter was proposed for reduction of multipath and noise interference. Multipath interference was first removed by cross-corre- lation filtering. Then the delays of each pulse in every echo were summed. An EMD algorithm was used for noise reduction for the total delay of each echo. The corresponding EMD results of every echo were then summed and averaged. Finally, evidence for the existence of buried life and their position were obtained from amplitude-frequency curves of the averaged EMD results. Detailed simulation experi- ments are presented to validate the effectiveness of this proposed method. The experimental results show that this method can efficiently eliminate multipath interference and reduce noise interference in echoes, which makes detection and location of buried life in coal mines more accurate.
文摘Low-cost multistoried rental housing （rumah susun sederhana sewa, abbreviated to rusunawa） recently has become the main policy for the Indonesian government to provide adequate housing for low income people in the metropolitan areas. In line with the policy, the government in the year 2005 has raised the technical life of building for low-cost multistoried rental housing from 30 years to 50 years. This study is conducted to assess variables that affect the technical life of architectural components, using Delphi study method involving stakeholders who have participated in the planning, design, construction, operation and maintenance of low-cost multistoried rental housing in Jakarta. Research findings contribute significantly to the assurance of technical life of architectural components in low-cost multistoried rental housing in Indonesia.
