AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma ...AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.展开更多
Common bean （Phaseolus vulgaris L.） is a staple food and income crop in eastern Africa, especially within the Uganda-Rwanda boarder region where it constitutes a major component of food security. Unfortunately, bush...Common bean （Phaseolus vulgaris L.） is a staple food and income crop in eastern Africa, especially within the Uganda-Rwanda boarder region where it constitutes a major component of food security. Unfortunately, bush beans are severely affected by frequent water-logging which persists over a considerable period of the year. In spite of this, farmers have continued to cultivate bush bean though with miserable yields. The study aimed to identify farmers＇ indigenous coping mechanisms to the climatic predicament, as a foundation for nurturing and subsequently designing appropriate management strategies for improved bean production in the region. A household survey was conducted in Kisoro district, in a location representative of the ecological conditions of Uganda-Rwanda region. 96 respondents were selected randomly at village level from a list of 500 households provided by the extension workers. Data were collected using semi-structured interviews. Findings showed that farmers possess vital coping mechanisms including construction of ridges, fertiliser application and chemical control of pests and diseases to expedite plant growth, and construction of drainage channels. It is clear that farmers＇ indigenous coping mechanisms need further nurturing and refining to improve their performance in dealing with water-logging crisis in the region.展开更多
The study explored the various performances of oral narratives in the Teso communities. In-depth interviews carried out with 68 respondents from 2009 to 2013, in six selected Teso districts in Uganda and Teso and Busi...The study explored the various performances of oral narratives in the Teso communities. In-depth interviews carried out with 68 respondents from 2009 to 2013, in six selected Teso districts in Uganda and Teso and Busia districts in Kenya, before and after performances, provided the data. In placing value and assessing the unquantifiable feelings of narrators and audiences, the study chose the methods of qualitative research and ethno-methodological philosophical analyses. Various levels of perceptions emerged from both the audiences and performers as they journeyed into both self and society. The study showed that the communities yearned for the communicative avenues of harnessing resources for solving various issues as they look into the future. Oral narratives motivated audiences through experiences of self discovery which spurred them to analogies of societal issues that haunted them. Both value and virtue were experienced at individual and group levels with a cultural identity and exposure to ethnic ties that bound them together in the struggle for a brighter tomorrow. The study recommends that a new society can be realized with movement from analogue to digital strategies for communication.展开更多
On today＇s clothes market and international fashion stage, the dress adornment elements of ethnic minorities have always deeply impressed design masters, received great attention and, are constantly surprising fashio...On today＇s clothes market and international fashion stage, the dress adornment elements of ethnic minorities have always deeply impressed design masters, received great attention and, are constantly surprising fashion designers. China is a country with multiple ethnic minorities, which have extremely rich ethnic minority dresses and adornment elements. On studying the major dress adornment elements of the Daur, a typical ethnic minority in northern China, we have discovered their strong ethnic features. The major dress adornment elements of the Daur were with the purpose to explore and continue traditions. Combining the modern faskion design with the delicate decorative art that the Daur people sew various laces and embroideries on their dresses, was to discover and use the essence of Chinese ethnic dress and adornments, and demonstrate the talent of ethnic minorities in clothes design. Given what have been founded, combined with the application of modern design methods, the adornment elements of ethnic dresses can represent their ethnic characteristics as well as reflecting the sense of the modern age.展开更多
This paper reveals the law of non-agriculture shift of the vulnerable groups in underdeveloped rural areas in china, and put forward the ＂second shift＂ category, namely the vulnerable groups is bound to work in citi...This paper reveals the law of non-agriculture shift of the vulnerable groups in underdeveloped rural areas in china, and put forward the ＂second shift＂ category, namely the vulnerable groups is bound to work in cities; return to participate in local non-agricultural activities; then begin second non-agriculture shift. The paper discusses the ＂second shift＂ feature; Finally, draw policy Implications of the ＂second shift＂.展开更多
The segmentation of the social protection system in China renders the system not only inequitable but inefficient.Those employed in the public sector,particularly civil servants,are＂overprotected＂,while workers in t...The segmentation of the social protection system in China renders the system not only inequitable but inefficient.Those employed in the public sector,particularly civil servants,are＂overprotected＂,while workers in the non-public sector,especially farmers and rural migrant workers,are＂under-protected＂.The segmented pension system impedes labor mobility while increasing administrative costs.Policies aimed at developing the new rural cooperative medical system and new rural old-age insurance scheme,as well as policies aimed at encouraging migrant workers to participate in urban social insurance programs,help improve the equity of social protection.Howere,competition between different local governments to win the ＂welfae crown＂ and the heavy brden placed on local finance caused by welface overspending has been hurting the sustainability of social protection programs.The following measures are suggested to address these problems;First,cease using social insurance coverage as an indicator of work performance for government officials;second,enhance the responsibility of the central government in social expenditure while increasing public transfers to less-developed regions in order to improve social assistance and merit goods provision;third,integrate or reorganized various sgements into one entity under each insurance program,with the purpose of eventually eliminating the segmentation problem in China＇s social protection system.展开更多
基金Supported by The National Natural Science Foundation of China, No. 30972898
文摘AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.
文摘Common bean （Phaseolus vulgaris L.） is a staple food and income crop in eastern Africa, especially within the Uganda-Rwanda boarder region where it constitutes a major component of food security. Unfortunately, bush beans are severely affected by frequent water-logging which persists over a considerable period of the year. In spite of this, farmers have continued to cultivate bush bean though with miserable yields. The study aimed to identify farmers＇ indigenous coping mechanisms to the climatic predicament, as a foundation for nurturing and subsequently designing appropriate management strategies for improved bean production in the region. A household survey was conducted in Kisoro district, in a location representative of the ecological conditions of Uganda-Rwanda region. 96 respondents were selected randomly at village level from a list of 500 households provided by the extension workers. Data were collected using semi-structured interviews. Findings showed that farmers possess vital coping mechanisms including construction of ridges, fertiliser application and chemical control of pests and diseases to expedite plant growth, and construction of drainage channels. It is clear that farmers＇ indigenous coping mechanisms need further nurturing and refining to improve their performance in dealing with water-logging crisis in the region.
文摘The study explored the various performances of oral narratives in the Teso communities. In-depth interviews carried out with 68 respondents from 2009 to 2013, in six selected Teso districts in Uganda and Teso and Busia districts in Kenya, before and after performances, provided the data. In placing value and assessing the unquantifiable feelings of narrators and audiences, the study chose the methods of qualitative research and ethno-methodological philosophical analyses. Various levels of perceptions emerged from both the audiences and performers as they journeyed into both self and society. The study showed that the communities yearned for the communicative avenues of harnessing resources for solving various issues as they look into the future. Oral narratives motivated audiences through experiences of self discovery which spurred them to analogies of societal issues that haunted them. Both value and virtue were experienced at individual and group levels with a cultural identity and exposure to ethnic ties that bound them together in the struggle for a brighter tomorrow. The study recommends that a new society can be realized with movement from analogue to digital strategies for communication.
文摘On today＇s clothes market and international fashion stage, the dress adornment elements of ethnic minorities have always deeply impressed design masters, received great attention and, are constantly surprising fashion designers. China is a country with multiple ethnic minorities, which have extremely rich ethnic minority dresses and adornment elements. On studying the major dress adornment elements of the Daur, a typical ethnic minority in northern China, we have discovered their strong ethnic features. The major dress adornment elements of the Daur were with the purpose to explore and continue traditions. Combining the modern faskion design with the delicate decorative art that the Daur people sew various laces and embroideries on their dresses, was to discover and use the essence of Chinese ethnic dress and adornments, and demonstrate the talent of ethnic minorities in clothes design. Given what have been founded, combined with the application of modern design methods, the adornment elements of ethnic dresses can represent their ethnic characteristics as well as reflecting the sense of the modern age.
文摘This paper reveals the law of non-agriculture shift of the vulnerable groups in underdeveloped rural areas in china, and put forward the ＂second shift＂ category, namely the vulnerable groups is bound to work in cities; return to participate in local non-agricultural activities; then begin second non-agriculture shift. The paper discusses the ＂second shift＂ feature; Finally, draw policy Implications of the ＂second shift＂.
文摘The segmentation of the social protection system in China renders the system not only inequitable but inefficient.Those employed in the public sector,particularly civil servants,are＂overprotected＂,while workers in the non-public sector,especially farmers and rural migrant workers,are＂under-protected＂.The segmented pension system impedes labor mobility while increasing administrative costs.Policies aimed at developing the new rural cooperative medical system and new rural old-age insurance scheme,as well as policies aimed at encouraging migrant workers to participate in urban social insurance programs,help improve the equity of social protection.Howere,competition between different local governments to win the ＂welfae crown＂ and the heavy brden placed on local finance caused by welface overspending has been hurting the sustainability of social protection programs.The following measures are suggested to address these problems;First,cease using social insurance coverage as an indicator of work performance for government officials;second,enhance the responsibility of the central government in social expenditure while increasing public transfers to less-developed regions in order to improve social assistance and merit goods provision;third,integrate or reorganized various sgements into one entity under each insurance program,with the purpose of eventually eliminating the segmentation problem in China＇s social protection system.
