Injectable bovine collagen has been used clinically for years. But both the necessity of repeated injections to maintain corrections and the question of adverse allergic reactions developing from the use of a xenogeni...Injectable bovine collagen has been used clinically for years. But both the necessity of repeated injections to maintain corrections and the question of adverse allergic reactions developing from the use of a xenogenic collagen have been an area of serious concern. To overoome these adverse effects, we have developed injectable collagen preparations from human placenta. Gamma irradiation was used for sterilization and crosslinkins of the collagen.We observed the mouse immune respose to gamma-irradiated human placenta soluble and insoluble collagen following multiple injections. After six injections of these materials, no total IgG level increase was found, nor was antibody specifically directed against human collagen found.Mouse antibody levels were also observed following Zyderm II and Zyplast repetitive injections and following repetitive implantations of coated vicryl and chromic gut. No humoral immune response was found in this heterologous type system.展开更多
AIM: Irritable bowel syndrome (IBS) is a functional bowel disorder. Its major symptom is bowel dysmotility, yet the mechanism of the symptom is poorly understood. Since the neurokinin-1 receptor (NK1R)-mediated signal...AIM: Irritable bowel syndrome (IBS) is a functional bowel disorder. Its major symptom is bowel dysmotility, yet the mechanism of the symptom is poorly understood. Since the neurokinin-1 receptor (NK1R)-mediated signaling in the gut is important in the control of normal bowel motor function,we aimed to investigate whether the NK1R-mediated bowel motor function was altered in IBS, using a rat IBS model that was previously reported to show colonic dysmotility in response to restraint stress.METHODS: IBS symptoms were produced in male SpragueDawley rats by inducing colitis with acetic acid. Rats were left to recover from colitis for 6 d, and used for experiments 7 d post-induction of colitis. Motor activities of distal colon were recorded in vitro.RESULTS: The contractile sensitivity of isolated colon to a NK1R agonist [Sar9, Met(O2)11]-substance P (1-30 nmol/L)was higher in IBS rats than that in normal rats. After the enteric neurotransmission was blocked by tetrodotoxin (TTX, 1 μmol/L), the contractile sensitivity to the NK1R agonist was increased in normal colon but not in IBS rat colon. The NK1R agonist-induced contraction was not different between the two groups when the agonist was challenged to the TTX-treated colon or the isolated colonic myocytes. A nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100 μmol/L) augmented the NK1R agonist-induced contraction only in normal rat colon.CONCLUSION: These results suggest that the NK1R-meidated colonic motor response is increased in IBS rats, due to the decrease in the nitrergic inhibitory neural component.展开更多
Allergens are innocuous environmental antigens that cause lgE mediated hypersensitivity reactions. These antigens can be divided into several groups according to their nature like plant allergens, grasses, fungal alle...Allergens are innocuous environmental antigens that cause lgE mediated hypersensitivity reactions. These antigens can be divided into several groups according to their nature like plant allergens, grasses, fungal allergens, pollen grains and house dust mite. Skin response to such allergens is associated with each other either by positive relation （positive correlation） or negative （inverse） relation. No previous papers found to discuss such correlations. In order to investigate these correlations, skin prick test of standard allergens solutions was carried out in 500 patients suffering allergic reactions of the age 12-60 years of both sexes. The results showed that there is a high significance of positive and negative correlations in skin response to allergens in the same group and with allergens of different groups suggesting that there is an induction or suppression of immune response. In conclusion the similarity or dissimilarity in the primary structure of some allergens in addition to gene-environment interaction may reflect positive/negative correlations between certain allergens that can influence the related allergic reactions.展开更多
Cashew nut hypersensitivity can cause severe reactions even life threatening. In the past few years,there are more cases of cashew nut allergy than before in Beijing. Thirty patients with cashew nut allergywere analyz...Cashew nut hypersensitivity can cause severe reactions even life threatening. In the past few years,there are more cases of cashew nut allergy than before in Beijing. Thirty patients with cashew nut allergywere analyzed in this paper. Case history, skin test, specific IgE and oral blinded challenge were used inthe diagnosis.展开更多
OBJECTIVE: To investigate bronchial responsiveness to acetylcholine in allergic airway inflammation of SD rats. METHODS: SD rats were immunized and challenged by chicken ovalbumin (OVA). Airway responsiveness, acetylc...OBJECTIVE: To investigate bronchial responsiveness to acetylcholine in allergic airway inflammation of SD rats. METHODS: SD rats were immunized and challenged by chicken ovalbumin (OVA). Airway responsiveness, acetylcholine (Ach) provocation concentration needed to increase baseline airway resistance by 200% (PC(200)) were measured. RESULTS: The value of baseline airway resistance in asthma group was significantly higher than that in control group (2.282 +/- 0.128 vs 3.193 +/- 0.239; P展开更多
As a global pollutant process, the reduction of mercury （Hg） is especially important. One pathway is through an abiotic reduction with humic acids （HAs）, which is controlled by different factors, including initial...As a global pollutant process, the reduction of mercury （Hg） is especially important. One pathway is through an abiotic reduction with humic acids （HAs）, which is controlled by different factors, including initial Hg and HA concentrations, pH, temperature and light. In this study, three humic acids were selected to illustrate the Hg2＋ abiotic reduction mechanisms by HAs, and to identify the key limiting factors for reduction rates and amounts. In addition, the initial status of the HAs as a solid or in an aqueous solution were also compared, to help explain why HAs show different dominant characteristics （e.g. complexation or reduction） in the reaction process with Hg. Results indicated that HAs were able to reduce Hg abiotically. Higher initial Hg, higher HA concentrations and either high （8.1） or low （3.6） solution pH decreased the HA reduction capacity. In addition, Hg~ production rates increased with increasing temperature, and the same trend was observed with light exposure. Humic acids added as an aqueous solution resulted in significantly greater HgO production than addition as a bulk solid. Finally, the Hg reduction rate and capacity varied significantly （P 〈 0.05） with HAs from different sources. These findings helped to explain why HAs showed different dominant characteristics （e.g. complexation or reduction） in the reaction process with of Hg2＋ reduction, which indicated that humic substances in sink or a source for Hg. Hg, and evidentially demonstrated the existence of a possible pathway natural environments, especially in water bodies, could act either as a展开更多
文摘Injectable bovine collagen has been used clinically for years. But both the necessity of repeated injections to maintain corrections and the question of adverse allergic reactions developing from the use of a xenogenic collagen have been an area of serious concern. To overoome these adverse effects, we have developed injectable collagen preparations from human placenta. Gamma irradiation was used for sterilization and crosslinkins of the collagen.We observed the mouse immune respose to gamma-irradiated human placenta soluble and insoluble collagen following multiple injections. After six injections of these materials, no total IgG level increase was found, nor was antibody specifically directed against human collagen found.Mouse antibody levels were also observed following Zyderm II and Zyplast repetitive injections and following repetitive implantations of coated vicryl and chromic gut. No humoral immune response was found in this heterologous type system.
文摘AIM: Irritable bowel syndrome (IBS) is a functional bowel disorder. Its major symptom is bowel dysmotility, yet the mechanism of the symptom is poorly understood. Since the neurokinin-1 receptor (NK1R)-mediated signaling in the gut is important in the control of normal bowel motor function,we aimed to investigate whether the NK1R-mediated bowel motor function was altered in IBS, using a rat IBS model that was previously reported to show colonic dysmotility in response to restraint stress.METHODS: IBS symptoms were produced in male SpragueDawley rats by inducing colitis with acetic acid. Rats were left to recover from colitis for 6 d, and used for experiments 7 d post-induction of colitis. Motor activities of distal colon were recorded in vitro.RESULTS: The contractile sensitivity of isolated colon to a NK1R agonist [Sar9, Met(O2)11]-substance P (1-30 nmol/L)was higher in IBS rats than that in normal rats. After the enteric neurotransmission was blocked by tetrodotoxin (TTX, 1 μmol/L), the contractile sensitivity to the NK1R agonist was increased in normal colon but not in IBS rat colon. The NK1R agonist-induced contraction was not different between the two groups when the agonist was challenged to the TTX-treated colon or the isolated colonic myocytes. A nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100 μmol/L) augmented the NK1R agonist-induced contraction only in normal rat colon.CONCLUSION: These results suggest that the NK1R-meidated colonic motor response is increased in IBS rats, due to the decrease in the nitrergic inhibitory neural component.
文摘Allergens are innocuous environmental antigens that cause lgE mediated hypersensitivity reactions. These antigens can be divided into several groups according to their nature like plant allergens, grasses, fungal allergens, pollen grains and house dust mite. Skin response to such allergens is associated with each other either by positive relation （positive correlation） or negative （inverse） relation. No previous papers found to discuss such correlations. In order to investigate these correlations, skin prick test of standard allergens solutions was carried out in 500 patients suffering allergic reactions of the age 12-60 years of both sexes. The results showed that there is a high significance of positive and negative correlations in skin response to allergens in the same group and with allergens of different groups suggesting that there is an induction or suppression of immune response. In conclusion the similarity or dissimilarity in the primary structure of some allergens in addition to gene-environment interaction may reflect positive/negative correlations between certain allergens that can influence the related allergic reactions.
文摘Cashew nut hypersensitivity can cause severe reactions even life threatening. In the past few years,there are more cases of cashew nut allergy than before in Beijing. Thirty patients with cashew nut allergywere analyzed in this paper. Case history, skin test, specific IgE and oral blinded challenge were used inthe diagnosis.
文摘OBJECTIVE: To investigate bronchial responsiveness to acetylcholine in allergic airway inflammation of SD rats. METHODS: SD rats were immunized and challenged by chicken ovalbumin (OVA). Airway responsiveness, acetylcholine (Ach) provocation concentration needed to increase baseline airway resistance by 200% (PC(200)) were measured. RESULTS: The value of baseline airway resistance in asthma group was significantly higher than that in control group (2.282 +/- 0.128 vs 3.193 +/- 0.239; P
基金Supported by the National Natural Science Foundation of China(No.40971147/D010503)the China Postdoctoral Science Foundation(No.2013M542238)+1 种基金the Fundamental Research Funds for The Central Universities,China(No.XDJK2013C151)the Ph.D.Initiation Fund of Southwest University,China(No.SWU112098)
文摘As a global pollutant process, the reduction of mercury （Hg） is especially important. One pathway is through an abiotic reduction with humic acids （HAs）, which is controlled by different factors, including initial Hg and HA concentrations, pH, temperature and light. In this study, three humic acids were selected to illustrate the Hg2＋ abiotic reduction mechanisms by HAs, and to identify the key limiting factors for reduction rates and amounts. In addition, the initial status of the HAs as a solid or in an aqueous solution were also compared, to help explain why HAs show different dominant characteristics （e.g. complexation or reduction） in the reaction process with Hg. Results indicated that HAs were able to reduce Hg abiotically. Higher initial Hg, higher HA concentrations and either high （8.1） or low （3.6） solution pH decreased the HA reduction capacity. In addition, Hg~ production rates increased with increasing temperature, and the same trend was observed with light exposure. Humic acids added as an aqueous solution resulted in significantly greater HgO production than addition as a bulk solid. Finally, the Hg reduction rate and capacity varied significantly （P 〈 0.05） with HAs from different sources. These findings helped to explain why HAs showed different dominant characteristics （e.g. complexation or reduction） in the reaction process with of Hg2＋ reduction, which indicated that humic substances in sink or a source for Hg. Hg, and evidentially demonstrated the existence of a possible pathway natural environments, especially in water bodies, could act either as a
