A Review] CD4+ T cells can be divided into Th1/Th2 subsets. Th1/Th2 imbalance participates many disease processes. A stable surface marker distinguishing Th1 and Th2 will greatly facilitate the investigation of Th1/Th...A Review] CD4+ T cells can be divided into Th1/Th2 subsets. Th1/Th2 imbalance participates many disease processes. A stable surface marker distinguishing Th1 and Th2 will greatly facilitate the investigation of Th1/Th2 interaction.Several surface molecules have been reported to be differentialy expressed between Th1 and Th2 cells.LAG-3,active ligands for P- and E-selectin ,IL-18R, IL-12Rβ2,CC chemokine receptor (CCR5) were shown to be dominantly expressed on Th1 cells,whereas expression of CD30,ST2L,CRTH2,CCR3,CCR4 was reported to be preferential to Th2 cells. In this review, several surface molecules were mainly discussed.展开更多
Pemphigus vulgaris (PV) is a blistering skin disorder mediated by autoantibodies targeting the epidermal adhesion molecule desmoglein 3 (Dsg3). As Th2-associated cytokines are necessary for directing antibody producti...Pemphigus vulgaris (PV) is a blistering skin disorder mediated by autoantibodies targeting the epidermal adhesion molecule desmoglein 3 (Dsg3). As Th2-associated cytokines are necessary for directing antibody production, it is hypothesized that Dsg3-specific Th2 activity is associated with active disease. We used cell-surface-matrix technology in combination with flow cytometry to characterize the Dsg3-reactive T-cell population using peripheral blood mononucleocytes sampled from PV patients stratified by active (n=9) or remittent disease (n=6),and healthy human leucocyte antigen-matched controls (n=5). We evaluated interferon-γ-producingCD4+cells (Th1) and interleukin (IL)-10-or IL-4-producing CD4+cells (Th2). The mean frequency of Th2 CD4+T cells was significantly elevated for five of nine PV patients with active disease. No significant Th2 responses were detected for patients with remittent disease or controls. There was a significant association of Th2 activity with active disease compared with remittent and control groups (P=0.026 and P = 0.012, respectively), and Th2 activity was significantly correlated with anti-Dsg3 IgG titre (P=0.044).One patient with remittent disease converted from a Th2-negative to a Th2-positive response with the initiation of disease activity. An antigen-specific CD4-lymphocyte response was detected in five PV patients (36%), and was shown to correlate closely with the CD8+population. These results are consistent with the hypothesis that Th2 response directs autoantibody production and is therefore associated with disease activity in PV.展开更多
基金国家自然科学基金! (No 3980 0 0 5 9)广东省自然科学基金! (No 980 46 0 )+1 种基金广东省医学科研课题!(A19982 33)广州市科委基础项
文摘A Review] CD4+ T cells can be divided into Th1/Th2 subsets. Th1/Th2 imbalance participates many disease processes. A stable surface marker distinguishing Th1 and Th2 will greatly facilitate the investigation of Th1/Th2 interaction.Several surface molecules have been reported to be differentialy expressed between Th1 and Th2 cells.LAG-3,active ligands for P- and E-selectin ,IL-18R, IL-12Rβ2,CC chemokine receptor (CCR5) were shown to be dominantly expressed on Th1 cells,whereas expression of CD30,ST2L,CRTH2,CCR3,CCR4 was reported to be preferential to Th2 cells. In this review, several surface molecules were mainly discussed.
文摘Pemphigus vulgaris (PV) is a blistering skin disorder mediated by autoantibodies targeting the epidermal adhesion molecule desmoglein 3 (Dsg3). As Th2-associated cytokines are necessary for directing antibody production, it is hypothesized that Dsg3-specific Th2 activity is associated with active disease. We used cell-surface-matrix technology in combination with flow cytometry to characterize the Dsg3-reactive T-cell population using peripheral blood mononucleocytes sampled from PV patients stratified by active (n=9) or remittent disease (n=6),and healthy human leucocyte antigen-matched controls (n=5). We evaluated interferon-γ-producingCD4+cells (Th1) and interleukin (IL)-10-or IL-4-producing CD4+cells (Th2). The mean frequency of Th2 CD4+T cells was significantly elevated for five of nine PV patients with active disease. No significant Th2 responses were detected for patients with remittent disease or controls. There was a significant association of Th2 activity with active disease compared with remittent and control groups (P=0.026 and P = 0.012, respectively), and Th2 activity was significantly correlated with anti-Dsg3 IgG titre (P=0.044).One patient with remittent disease converted from a Th2-negative to a Th2-positive response with the initiation of disease activity. An antigen-specific CD4-lymphocyte response was detected in five PV patients (36%), and was shown to correlate closely with the CD8+population. These results are consistent with the hypothesis that Th2 response directs autoantibody production and is therefore associated with disease activity in PV.
