目的本文建立食品中鲍鱼过敏源基因成分实时荧光PCR检测方法。方法采用蛋白酶K消化法提取鲍鱼肌肉组织中基因组DNA,针对鲍鱼管家基因16S r RNA基因设计特异性引物和探针,确定实时荧光PCR反应体系和反应条件,建立了鲍鱼过敏源基因成分实...目的本文建立食品中鲍鱼过敏源基因成分实时荧光PCR检测方法。方法采用蛋白酶K消化法提取鲍鱼肌肉组织中基因组DNA,针对鲍鱼管家基因16S r RNA基因设计特异性引物和探针,确定实时荧光PCR反应体系和反应条件,建立了鲍鱼过敏源基因成分实时荧光PCR快速检测方法。选用鱿鱼、海参等海产品进行特异性试验;采用添加方法制备灵敏度试验样品,分别制备了鲍鱼过敏源基因成分含量分别为100%、10%、1%、0.1%、0.01%、0.001%的样品。结果对非鲍鱼类食品进行检测,结果显示出良好的特异性;灵敏度试验表明,本文建立方法的最低检测下限为0.01%。结论本文建立了特异性好,灵敏度高的鲍鱼过敏源基因成分检测方法。展开更多
Products of plant resistance ( R ) genes Pto and Cf contain distinct domains, and have different cellular localization. It is intriguing to compare the development mechanisms of resistance conferred by the t...Products of plant resistance ( R ) genes Pto and Cf contain distinct domains, and have different cellular localization. It is intriguing to compare the development mechanisms of resistance conferred by the two R genes. In the present report, two hypersensitive response (HR) initiation systems were employed to study the time_course expression induced by Avr / Cf interaction of the genes encoding Pti4, Pti5 and Pti6 which interact directly with Pto: (1) Seeds of tomato (Lycopersicon esculentum Mill.) containing complementary gene pairs Avr 4/ Cf _4 and Avr 9/ Cf _9 were obtained through crossing. Their seedlings developed HR under room temperature. (2) Avr / Cf seedlings grew normally at 33 ℃. When the temperature was shifted down to 25 ℃, HR occurred within hours in the seedlings. Results of both experiments showed that expression of Pti4, Pti5 and Pti6 was induced upon development of hypersensitive necrosis in Avr / Cf seedlings. However, the expression levels and patterns of these Pti s differed. This finding indicated that these Pti s function complementarily, and might be involved in regulation of both Pto and Cf _conferred resistance.展开更多
Celiac disease(CD) is one of the most common diseases,resulting from both environmental(gluten) and genetic factors [human leukocyte antigen(HLA) and nonHLA genes].The prevalence of CD has been estimated to approximat...Celiac disease(CD) is one of the most common diseases,resulting from both environmental(gluten) and genetic factors [human leukocyte antigen(HLA) and nonHLA genes].The prevalence of CD has been estimated to approximate 0.5%-1% in different parts of the world.However,the population with diabetes,autoimmune disorder or relatives of CD individuals have even higher risk for the development of CD,at least in part,because of shared HLA typing.Gliadin gains access to the basal surface of the epithelium,and interact directly with the immune system,via both trans-and para-cellular routes.From a diagnostic perspective,symptoms may be viewed as either "typical" or "atypical".In both positive serological screening results suggestive of CD,should lead to small bowel biopsy followed by a favourable clinical and serological response to the gluten-free diet(GFD) to confirm the diagnosis.Positive anti-tissue transglutaminase antibody or antiendomysial antibody during the clinical course helps to confirm the diagnosis of CD because of their over 99% specificities when small bowel villous atrophy is present on biopsy.Currently,the only treatment available for CD individuals is a strict life-long GFD.A greater understanding of the pathogenesis of CD allows alternative future CD treatments to hydrolyse toxic gliadin peptide,prevent toxic gliadin peptide absorption,blockage of selective deamidation of specific glutamine residues by tissue,restore immune tolerance towards gluten,modulation of immune response to dietary gliadin,and restoration of intestinal architecture.展开更多
文摘目的本文建立食品中鲍鱼过敏源基因成分实时荧光PCR检测方法。方法采用蛋白酶K消化法提取鲍鱼肌肉组织中基因组DNA,针对鲍鱼管家基因16S r RNA基因设计特异性引物和探针,确定实时荧光PCR反应体系和反应条件,建立了鲍鱼过敏源基因成分实时荧光PCR快速检测方法。选用鱿鱼、海参等海产品进行特异性试验;采用添加方法制备灵敏度试验样品,分别制备了鲍鱼过敏源基因成分含量分别为100%、10%、1%、0.1%、0.01%、0.001%的样品。结果对非鲍鱼类食品进行检测,结果显示出良好的特异性;灵敏度试验表明,本文建立方法的最低检测下限为0.01%。结论本文建立了特异性好,灵敏度高的鲍鱼过敏源基因成分检测方法。
文摘Products of plant resistance ( R ) genes Pto and Cf contain distinct domains, and have different cellular localization. It is intriguing to compare the development mechanisms of resistance conferred by the two R genes. In the present report, two hypersensitive response (HR) initiation systems were employed to study the time_course expression induced by Avr / Cf interaction of the genes encoding Pti4, Pti5 and Pti6 which interact directly with Pto: (1) Seeds of tomato (Lycopersicon esculentum Mill.) containing complementary gene pairs Avr 4/ Cf _4 and Avr 9/ Cf _9 were obtained through crossing. Their seedlings developed HR under room temperature. (2) Avr / Cf seedlings grew normally at 33 ℃. When the temperature was shifted down to 25 ℃, HR occurred within hours in the seedlings. Results of both experiments showed that expression of Pti4, Pti5 and Pti6 was induced upon development of hypersensitive necrosis in Avr / Cf seedlings. However, the expression levels and patterns of these Pti s differed. This finding indicated that these Pti s function complementarily, and might be involved in regulation of both Pto and Cf _conferred resistance.
文摘Celiac disease(CD) is one of the most common diseases,resulting from both environmental(gluten) and genetic factors [human leukocyte antigen(HLA) and nonHLA genes].The prevalence of CD has been estimated to approximate 0.5%-1% in different parts of the world.However,the population with diabetes,autoimmune disorder or relatives of CD individuals have even higher risk for the development of CD,at least in part,because of shared HLA typing.Gliadin gains access to the basal surface of the epithelium,and interact directly with the immune system,via both trans-and para-cellular routes.From a diagnostic perspective,symptoms may be viewed as either "typical" or "atypical".In both positive serological screening results suggestive of CD,should lead to small bowel biopsy followed by a favourable clinical and serological response to the gluten-free diet(GFD) to confirm the diagnosis.Positive anti-tissue transglutaminase antibody or antiendomysial antibody during the clinical course helps to confirm the diagnosis of CD because of their over 99% specificities when small bowel villous atrophy is present on biopsy.Currently,the only treatment available for CD individuals is a strict life-long GFD.A greater understanding of the pathogenesis of CD allows alternative future CD treatments to hydrolyse toxic gliadin peptide,prevent toxic gliadin peptide absorption,blockage of selective deamidation of specific glutamine residues by tissue,restore immune tolerance towards gluten,modulation of immune response to dietary gliadin,and restoration of intestinal architecture.
